Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

11-cis retinol dehydrogenase

Gene

RDH5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all-trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP.3 Publications

Catalytic activityi

11-cis-retinol-[retinal-binding-protein] + NAD+ = 11-cis-retinal-[retinol-binding-protein] + NADH.3 Publications

Enzyme regulationi

Inhibited by 9-cis-, 13-cis- and all-trans-retinoic acids, with the most potent inhibitor being 13-cis-retinoic acid. Weakly inhibited by oleic acid (By similarity).By similarity

Pathwayi: retinol metabolism

This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631SubstrateBy similarity
Active sitei175 – 1751Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 5625NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.315. 908.
ReactomeiR-BTA-2453902. The canonical retinoid cycle in rods (twilight vision).
R-BTA-5365859. RA biosynthesis pathway.
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
11-cis retinol dehydrogenase (EC:1.1.1.315)
Short name:
11-cis RDH
Short name:
11-cis RoDH
Alternative name(s):
9-cis retinol dehydrogenase
Short name:
9cRDH
P32
Gene namesi
Name:RDH5
Synonyms:RDH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componentsi: Chromosome 5, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 31829511-cis retinol dehydrogenasePRO_0000054757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ27979.
PRIDEiQ27979.

Expressioni

Tissue specificityi

Expressed in retinal pigment epithelial cells. Expression detected in smooth muscle cells of the small arteries in liver, kidney, small intestine and heart, and in small intestine also in the muscularis mucosae and muscularis propria (at protein level; PubMed:9654122). Expressed in eye in retinal pigment epithelium but not in lens capsule, iris, retina, cornea epithelium and ciliary body. Not detected in adrenal gland, lung, testis, brain, muscle and spleen. Not detected in liver, kidney, heart and small intestine (PubMed:7544779 and PubMed:7836368).3 Publications

Gene expression databases

ExpressionAtlasiQ27979. baseline.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000056512.

Structurei

3D structure databases

ProteinModelPortaliQ27979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1610. Eukaryota.
ENOG410Y7FK. LUCA.
GeneTreeiENSGT00840000129713.
HOVERGENiHBG005482.
InParanoidiQ27979.
KOiK00061.
OMAiTLQACWA.
OrthoDBiEOG7FXZZX.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR032968. RDH5.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 1 hit.
PTHR24322:SF605. PTHR24322:SF605. 1 hit.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLPLLLGVL LWAALWLLRD RQCLPASDAF IFITGCDSGF GRLLALRLDQ
60 70 80 90 100
RGFRVLASCL TPSGAEDLQR VASSRLHTTL LDVTDPQSIR QAVKWVETHV
110 120 130 140 150
GEAGLFGLVN NAGVAGIIGP TPWQTREDFQ RVLNVNTLGP IGVTLALLPL
160 170 180 190 200
LLQARGRVIN ITSVLGRLAA NGGGYCVSKF GLEAFSDSLR RDVAPFGVRV
210 220 230 240 250
SIVEPGFFRT PVTNLETLED TLQACWARLP PATQALYGEA FLTKYLRVQQ
260 270 280 290 300
RIMNMICDPD LAKVSRCLEH ALTARHPRTR YSPGWDAKLL WLPASYLPAR
310
LVDAVLAWVL PKPAQTVY
Length:318
Mass (Da):35,037
Last modified:November 1, 1996 - v1
Checksum:i78B4A1E43E5FB351
GO

Sequence cautioni

The sequence AAA80694.1 differs from that shown. Reason: Frameshift at positions 2, 25 and 29. Curated
The sequence DAAA02013541 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36533 mRNA. Translation: AAA80694.1. Frameshift.
X82262 mRNA. Translation: CAA57715.1.
DAAA02013541 Genomic DNA. No translation available.
PIRiA55429.
I45845.
RefSeqiNP_776591.2. NM_174166.3.
XP_010803419.1. XM_010805117.2.
UniGeneiBt.4598.

Genome annotation databases

EnsembliENSBTAT00000065932; ENSBTAP00000056512; ENSBTAG00000011927.
GeneIDi281448.
KEGGibta:281448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36533 mRNA. Translation: AAA80694.1. Frameshift.
X82262 mRNA. Translation: CAA57715.1.
DAAA02013541 Genomic DNA. No translation available.
PIRiA55429.
I45845.
RefSeqiNP_776591.2. NM_174166.3.
XP_010803419.1. XM_010805117.2.
UniGeneiBt.4598.

3D structure databases

ProteinModelPortaliQ27979.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000056512.

Proteomic databases

PaxDbiQ27979.
PRIDEiQ27979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000065932; ENSBTAP00000056512; ENSBTAG00000011927.
GeneIDi281448.
KEGGibta:281448.

Organism-specific databases

CTDi5959.

Phylogenomic databases

eggNOGiKOG1610. Eukaryota.
ENOG410Y7FK. LUCA.
GeneTreeiENSGT00840000129713.
HOVERGENiHBG005482.
InParanoidiQ27979.
KOiK00061.
OMAiTLQACWA.
OrthoDBiEOG7FXZZX.
TreeFamiTF325617.

Enzyme and pathway databases

UniPathwayiUPA00912.
BRENDAi1.1.1.315. 908.
ReactomeiR-BTA-2453902. The canonical retinoid cycle in rods (twilight vision).
R-BTA-5365859. RA biosynthesis pathway.

Gene expression databases

ExpressionAtlasiQ27979. baseline.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR032968. RDH5.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 1 hit.
PTHR24322:SF605. PTHR24322:SF605. 1 hit.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase."
    Driessen C.A., Janssen B.P., Winkens H.J., van Vugt A.H., de Leeuw T.L., Janssen J.J.
    Invest. Ophthalmol. Vis. Sci. 36:1988-1996(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Retinal pigment epithelium.
  2. "The retinal pigment epithelial-specific 11-cis retinol dehydrogenase belongs to the family of short chain alcohol dehydrogenases."
    Simon A., Hellman U., Wernstedt C., Eriksson U.
    J. Biol. Chem. 270:1107-1112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 76-89; 192-198; 210-224; 281-288 AND 301-318, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Retinal pigment epithelium.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  4. "The visual cycle retinol dehydrogenase: possible involvement in the 9-cis retinoic acid biosynthetic pathway."
    Driessen C.A., Winkens H.J., Kuhlmann E.D., Janssen A.P., van Vugt A.H., Deutman A.F., Janssen J.J.
    FEBS Lett. 428:135-140(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiRDH1_BOVIN
AccessioniPrimary (citable) accession number: Q27979
Secondary accession number(s): F1ML51, Q28004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.