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Protein

Heat shock 70 kDa protein 1A

Gene

HSPA1A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154ATPBy similarity
Nucleotide bindingi202 – 2043ATPBy similarity
Nucleotide bindingi268 – 2758ATPBy similarity
Nucleotide bindingi339 – 3424ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 1A
Alternative name(s):
Heat shock 70 kDa protein 1
Short name:
HSP70.1
Gene namesi
Name:HSPA1A
Synonyms:HSP70-1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 641640Heat shock 70 kDa protein 1APRO_0000078245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei108 – 1081N6-acetyllysineBy similarity
Modified residuei246 – 2461N6-acetyllysineBy similarity
Modified residuei348 – 3481N6-acetyllysineBy similarity
Modified residuei561 – 5611N6,N6,N6-trimethyllysine; by METTL21A; in vitroBy similarity
Modified residuei631 – 6311PhosphoserineBy similarity
Modified residuei633 – 6331PhosphoserineBy similarity
Modified residuei636 – 6361PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ27975.
PRIDEiQ27975.

Expressioni

Inductioni

By heat shock.

Interactioni

Subunit structurei

Component of the CatSper complex. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with CHCHD3, DNAJC7, IRAK1BP1, PPP5C and TSC2. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with TRIM5 (via B30.2/SPRY domain). Interacts with METTL21A. Interacts with DNAAF2. Interacts with PARK2. Interacts with FOXP3. Interacts with NOD2; the interaction enhances NOD2 stability. Interacts with DNAJC9 (via J domain). Interacts with ATF5; the interaction protects ATF5 from degradation via proteasome-dependent and caspase-dependent processes (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
S100A1P026392EBI-6477341,EBI-6477285

Protein-protein interaction databases

BioGridi159138. 1 interaction.
IntActiQ27975. 1 interaction.
STRINGi9913.ENSBTAP00000017500.

Structurei

3D structure databases

ProteinModelPortaliQ27975.
SMRiQ27975. Positions 1-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00840000130013.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiQ27975.
KOiK03283.
OMAiCSETIAW.
OrthoDBiEOG7PCJGF.
TreeFamiTF105042.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNMAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL
60 70 80 90 100
IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFRVINDGDK
110 120 130 140 150
PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGHPVT NAVITVPAYF
160 170 180 190 200
NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL
210 220 230 240 250
GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK
260 270 280 290 300
KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA
310 320 330 340 350
RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL
360 370 380 390 400
QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
410 420 430 440 450
LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT
460 470 480 490 500
RDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK
510 520 530 540 550
ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK
560 570 580 590 600
SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE
610 620 630 640
QVCNPIISRL YQGAGGPGAG GFGAQGPKGG SGSGPTIEEV D
Length:641
Mass (Da):70,259
Last modified:December 21, 2004 - v2
Checksum:i6FAC1F30540E80F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571N → S in AAT75223 (PubMed:15491140).Curated
Sequence conflicti89 – 891H → E in AAA73914 (PubMed:7826329).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09861 mRNA. Translation: AAA73914.1.
AY149618 Genomic DNA. Translation: AAN78092.1.
AY149619 Genomic DNA. Translation: AAN78094.1.
AY662497 mRNA. Translation: AAT75223.1.
U02891 Unassigned DNA. Translation: AAA03450.1.
PIRiS53357.
RefSeqiNP_776975.1. NM_174550.1.
UniGeneiBt.49659.

Genome annotation databases

EnsembliENSBTAT00000017500; ENSBTAP00000017500; ENSBTAG00000025441.
GeneIDi281825.
KEGGibta:281825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09861 mRNA. Translation: AAA73914.1.
AY149618 Genomic DNA. Translation: AAN78092.1.
AY149619 Genomic DNA. Translation: AAN78094.1.
AY662497 mRNA. Translation: AAT75223.1.
U02891 Unassigned DNA. Translation: AAA03450.1.
PIRiS53357.
RefSeqiNP_776975.1. NM_174550.1.
UniGeneiBt.49659.

3D structure databases

ProteinModelPortaliQ27975.
SMRiQ27975. Positions 1-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159138. 1 interaction.
IntActiQ27975. 1 interaction.
STRINGi9913.ENSBTAP00000017500.

Proteomic databases

PaxDbiQ27975.
PRIDEiQ27975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017500; ENSBTAP00000017500; ENSBTAG00000025441.
GeneIDi281825.
KEGGibta:281825.

Organism-specific databases

CTDi3303.

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00840000130013.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiQ27975.
KOiK03283.
OMAiCSETIAW.
OrthoDBiEOG7PCJGF.
TreeFamiTF105042.

Miscellaneous databases

NextBioi20805735.
PROiQ27975.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chemical modifications of a recombinant bovine stress-inducible 70 kDa heat-shock protein (Hsp70) mimics Hsp70 isoforms from tissues."
    Gutierrez J.A., Guerriero V.
    Biochem. J. 305:197-203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. "Deletion of one of the duplicated Hsp70 genes causes hereditary myopathy of diaphragmatic muscles in Holstein-Friesian cattle."
    Sugimoto M., Furuoka H., Sugimoto Y.
    Anim. Genet. 34:191-197(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Holstein-Friesian.
  3. "Molecular cloning of bovine cardiac muscle heat-shock protein 70 kDa and its phosphorylation by cAMP-dependent protein kinase in vitro."
    Lakshmikuttyamma A., Selvakumar P., Anderson D.H., Datla R.S., Sharma R.K.
    Biochemistry 43:13340-13347(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart muscle.
  4. "An AluI polymorphism at the bovine 70 kD heat-shock protein-1 (HSP70-1) locus."
    Grosz M.D., Skow L.C., Stone R.T.
    Anim. Genet. 25:196-196(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 212-641.
    Strain: Angus.

Entry informationi

Entry nameiHS71A_BOVIN
AccessioniPrimary (citable) accession number: Q27975
Secondary accession number(s): Q27964, Q6DLW1, Q7YQF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 21, 2004
Last modified: May 11, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.