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Q27974

- AUXI_BOVIN

UniProt

Q27974 - AUXI_BOVIN

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Protein
Putative tyrosine-protein phosphatase auxilin
Gene
DNAJC6
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons By similarity.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611Phosphocysteine intermediate Reviewed prediction

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Chaperone, Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative tyrosine-protein phosphatase auxilin (EC:3.1.3.48)
    Alternative name(s):
    DnaJ homolog subfamily C member 6
    Gene namesi
    Name:DNAJC6
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi847 – 8471K → C: Strongly reduces interaction with HSPA8. 1 Publication
    Mutagenesisi849 – 8491K → C: Slightly reduces interaction with HSPA8. 1 Publication
    Mutagenesisi876 – 8761D → A: Loss of interaction with HSPA8. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 910910Putative tyrosine-protein phosphatase auxilin
    PRO_0000215902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei560 – 5601Phosphoserine By similarity
    Modified residuei567 – 5671Phosphoserine By similarity

    Post-translational modificationi

    The N-terminus is blocked.
    Target for coat-associated casein kinase II in vitro.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ27974.

    Expressioni

    Tissue specificityi

    Brain.

    Interactioni

    Subunit structurei

    Interacts with HSPA8/HSC70. Interacts with clathrin heavy chains.1 Publication

    Protein-protein interaction databases

    DIPiDIP-41952N.
    MINTiMINT-205318.
    STRINGi9913.ENSBTAP00000021828.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi54 – 607
    Beta strandi62 – 7413
    Helixi89 – 9911
    Beta strandi103 – 1075
    Beta strandi109 – 1113
    Helixi114 – 1163
    Helixi119 – 1213
    Beta strandi122 – 1243
    Beta strandi129 – 1313
    Helixi135 – 15117
    Beta strandi156 – 1616
    Helixi166 – 17813
    Helixi185 – 19511
    Helixi203 – 21614
    Beta strandi217 – 2193
    Beta strandi228 – 23710
    Beta strandi245 – 2484
    Beta strandi250 – 2567
    Beta strandi259 – 2635
    Helixi268 – 2703
    Helixi276 – 2783
    Beta strandi281 – 2899
    Beta strandi291 – 30414
    Beta strandi310 – 32314
    Helixi324 – 3263
    Beta strandi333 – 3375
    Helixi338 – 3403
    Helixi347 – 3493
    Beta strandi355 – 3628
    Helixi374 – 3774
    Helixi385 – 3884
    Helixi392 – 3987
    Beta strandi738 – 7403
    Beta strandi751 – 7544
    Beta strandi757 – 7593
    Helixi781 – 7833
    Beta strandi786 – 7894
    Helixi801 – 8099
    Helixi814 – 82613
    Helixi830 – 8367
    Helixi837 – 8393
    Helixi853 – 8553
    Beta strandi856 – 8583
    Helixi859 – 87214
    Helixi875 – 8784
    Helixi884 – 90320

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N4CNMR-A737-910[»]
    1NZ6X-ray2.50A/B810-910[»]
    1Q2Gmodel-A776-910[»]
    1XI5electron microscopy12.00J/K/L/M/N/O/P/Q/R797-910[»]
    2QWNX-ray2.40B812-905[»]
    2QWOX-ray1.70B813-904[»]
    2QWPX-ray1.75B813-904[»]
    2QWQX-ray2.21B813-904[»]
    2QWRX-ray2.21B813-904[»]
    3N0AX-ray2.20A40-400[»]
    DisProtiDP00351.
    ProteinModelPortaliQ27974.
    SMRiQ27974. Positions 734-910.

    Miscellaneous databases

    EvolutionaryTraceiQ27974.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati33 – 3641
    Repeati37 – 4042
    Repeati41 – 4443
    Domaini52 – 219168Phosphatase tensin-type
    Add
    BLAST
    Domaini225 – 363139C2 tensin-type
    Add
    BLAST
    Domaini846 – 91065J
    Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni33 – 44123 X 4 AA approximate tandem repeats
    Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi406 – 4149SH3-binding Reviewed prediction

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi463 – 757295Pro-rich
    Add
    BLAST
    Compositional biasi526 – 5294Poly-Gly

    Sequence similaritiesi

    Contains 1 J domain.

    Keywords - Domaini

    Repeat, SH3-binding

    Phylogenomic databases

    eggNOGiNOG269956.
    HOGENOMiHOG000034235.
    HOVERGENiHBG004322.
    InParanoidiQ27974.
    KOiK09526.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view]
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    PROSITEiPS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27974-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSSGASSPD MEPSYGGGLF DMVKGGAGRL FSNLKDNLKD TLKDTSSRVI    50
    QSVTSYTKGD LDFTYVTSRI IVMSFPLDSV DIGFRNQVDD IRSFLDSRHL 100
    DHYTVYNLSP KSYRTAKFHS RVSECSWPIR QAPSLHNLFA VCRNMYNWLL 150
    QNPKNVCVVH CLDGRAASSI LVGAMFIFCN LYSTPGPAVR LLYAKRPGIG 200
    LSPSHRRYLG YMCDLLADKP YRPHFKPLTI KSITVSPVPF FNKQRNGCRP 250
    YCDVLIGETK IYTTCADFER MKEYRVQDGK IFIPLSITVQ GDVVVSMYHL 300
    RSTIGSRLQA KVTNTQIFQL QFHTGFIPLD TTVLKFTKPE LDACDVPEKY 350
    PQLFQVTLDV ELQPHDKVME LTPPWEHYCT KDVNPSILFS SHQEHQDTLV 400
    LGGQAPIDIP PDNPRHFGQG GFFSTLCWQD QKSEKSFCEE DHAALVNQES 450
    EQSDDELLTL SSPHGNANGD KPHAARKPSK KQQEPAAPAP PEDVDLLGLE 500
    GSAVSKNFSS PAAPPSNSEL LSDLFGGGGA AGPVQSGQSG VDDVFHPSGP 550
    TSTQSTPRRS ATSTSASPTL RVGEGATFDP FGAPSKPSGQ DLLGSFLNTA 600
    SASSDPFLQP TRSPSPTVHA SSTPAVNIQP DVSGAWDWHT KPGGFGMGSK 650
    SAATSPTGSS HGTPTHQNKP QTLDPFADLG TLGGSSFASK PSTPTGLGGG 700
    FPPLSSPQKA SPQPMGGGWQ QGGGYNWQQT QSKPQSSMPH SSPQNRPNYN 750
    VSFSSMPGGQ NERGKAAANL EGKQKAADFE DLLSGQGFNA HKDKKGPRTI 800
    AEMRKEEMAK EMDPEKLKIL EWIEGKERNI RALLSTMHTV LWAGETKWKP 850
    VGMADLVTPE QVKKVYRKAV LVVHPDKATG QPYEQYAKMI FMELNDAWSE 900
    FENQGQKPLY 910
    Length:910
    Mass (Da):99,512
    Last modified:November 1, 1997 - v1
    Checksum:iBC156DC1CF3487FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09237 mRNA. Translation: AAA79037.1.
    PIRiS68983.
    RefSeqiNP_777261.1. NM_174836.2.
    UniGeneiBt.111572.

    Genome annotation databases

    GeneIDi317659.
    KEGGibta:317659.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09237 mRNA. Translation: AAA79037.1 .
    PIRi S68983.
    RefSeqi NP_777261.1. NM_174836.2.
    UniGenei Bt.111572.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N4C NMR - A 737-910 [» ]
    1NZ6 X-ray 2.50 A/B 810-910 [» ]
    1Q2G model - A 776-910 [» ]
    1XI5 electron microscopy 12.00 J/K/L/M/N/O/P/Q/R 797-910 [» ]
    2QWN X-ray 2.40 B 812-905 [» ]
    2QWO X-ray 1.70 B 813-904 [» ]
    2QWP X-ray 1.75 B 813-904 [» ]
    2QWQ X-ray 2.21 B 813-904 [» ]
    2QWR X-ray 2.21 B 813-904 [» ]
    3N0A X-ray 2.20 A 40-400 [» ]
    DisProti DP00351.
    ProteinModelPortali Q27974.
    SMRi Q27974. Positions 734-910.
    ModBasei Search...

    Protein-protein interaction databases

    DIPi DIP-41952N.
    MINTi MINT-205318.
    STRINGi 9913.ENSBTAP00000021828.

    Proteomic databases

    PRIDEi Q27974.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 317659.
    KEGGi bta:317659.

    Organism-specific databases

    CTDi 9829.

    Phylogenomic databases

    eggNOGi NOG269956.
    HOGENOMi HOG000034235.
    HOVERGENi HBG004322.
    InParanoidi Q27974.
    KOi K09526.

    Miscellaneous databases

    EvolutionaryTracei Q27974.
    NextBioi 20807135.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view ]
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    PROSITEi PS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the neuronal clathrin-associated protein auxilin and its expression in bacteria."
      Schroeder S., Morris S.A., Knorr R., Plessmann U., Weber K., Vinh N.G., Ungewickell E.
      Eur. J. Biochem. 228:297-304(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Brain.
    2. "1H, 15N, and 13C NMR backbone assignments and secondary structure of the C-terminal recombinant fragment of auxilin including the J-domain."
      Han C.J., Gruschus J.M., Greener T., Greene L.E., Ferretti J., Eisenberg E.
      J. Biomol. NMR 17:281-282(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 713-910.
    3. "Structure-function analysis of the auxilin J-domain reveals an extended Hsc70 interaction interface."
      Jiang J., Taylor A.B., Prasad K., Ishikawa-Brush Y., Hart P.J., Lafer E.M., Sousa R.
      Biochemistry 42:5748-5753(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 810-910, MUTAGENESIS OF LYS-847; LYS-849 AND ASP-876, INTERACTION WITH HSPA8.
    4. "Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating."
      Fotin A., Cheng Y., Grigorieff N., Walz T., Harrison S.C., Kirchhausen T.
      Nature 432:649-653(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS) OF 797-910 IN COMPLEX WITH CLATHRIN COATS, FUNCTION.

    Entry informationi

    Entry nameiAUXI_BOVIN
    AccessioniPrimary (citable) accession number: Q27974
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: June 11, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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