Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative tyrosine-protein phosphatase auxilin

Gene

DNAJC6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons (By similarity).By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tyrosine-protein phosphatase auxilin (EC:3.1.3.48)
Alternative name(s):
DnaJ homolog subfamily C member 6
Gene namesi
Name:DNAJC6
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi847K → C: Strongly reduces interaction with HSPA8. 1 Publication1
Mutagenesisi849K → C: Slightly reduces interaction with HSPA8. 1 Publication1
Mutagenesisi876D → A: Loss of interaction with HSPA8. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002159021 – 910Putative tyrosine-protein phosphatase auxilinAdd BLAST910

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109PhosphoserineBy similarity1
Modified residuei450PhosphoserineBy similarity1
Modified residuei453PhosphoserineBy similarity1
Modified residuei560PhosphoserineBy similarity1
Modified residuei567PhosphoserineBy similarity1

Post-translational modificationi

The N-terminus is blocked.
Target for coat-associated casein kinase II in vitro.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ27974.
PRIDEiQ27974.

Expressioni

Tissue specificityi

Brain.

Interactioni

Subunit structurei

Interacts with HSPA8/HSC70. Interacts with clathrin heavy chains.1 Publication

Protein-protein interaction databases

DIPiDIP-41952N.
MINTiMINT-205318.
STRINGi9913.ENSBTAP00000041032.

Structurei

Secondary structure

1910
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi54 – 60Combined sources7
Beta strandi62 – 74Combined sources13
Helixi89 – 99Combined sources11
Beta strandi103 – 107Combined sources5
Beta strandi109 – 111Combined sources3
Helixi114 – 116Combined sources3
Helixi119 – 121Combined sources3
Beta strandi122 – 124Combined sources3
Beta strandi129 – 131Combined sources3
Helixi135 – 151Combined sources17
Beta strandi156 – 161Combined sources6
Helixi166 – 178Combined sources13
Helixi185 – 195Combined sources11
Helixi203 – 216Combined sources14
Beta strandi217 – 219Combined sources3
Beta strandi228 – 237Combined sources10
Beta strandi245 – 248Combined sources4
Beta strandi250 – 256Combined sources7
Beta strandi259 – 263Combined sources5
Helixi268 – 270Combined sources3
Helixi276 – 278Combined sources3
Beta strandi281 – 289Combined sources9
Beta strandi291 – 304Combined sources14
Beta strandi310 – 323Combined sources14
Helixi324 – 326Combined sources3
Beta strandi333 – 337Combined sources5
Helixi338 – 340Combined sources3
Helixi347 – 349Combined sources3
Beta strandi355 – 362Combined sources8
Helixi374 – 377Combined sources4
Helixi385 – 388Combined sources4
Helixi392 – 398Combined sources7
Beta strandi738 – 740Combined sources3
Beta strandi751 – 754Combined sources4
Beta strandi757 – 759Combined sources3
Helixi781 – 783Combined sources3
Beta strandi786 – 789Combined sources4
Helixi801 – 809Combined sources9
Helixi814 – 826Combined sources13
Helixi830 – 836Combined sources7
Helixi837 – 839Combined sources3
Helixi853 – 855Combined sources3
Beta strandi856 – 858Combined sources3
Helixi859 – 872Combined sources14
Helixi875 – 878Combined sources4
Helixi884 – 903Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4CNMR-A737-910[»]
1NZ6X-ray2.50A/B810-910[»]
1Q2Gmodel-A776-910[»]
1XI5electron microscopy12.00J/K/L/M/N/O/P/Q/R797-910[»]
2QWNX-ray2.40B812-905[»]
2QWOX-ray1.70B813-904[»]
2QWPX-ray1.75B813-904[»]
2QWQX-ray2.21B813-904[»]
2QWRX-ray2.21B813-904[»]
3N0AX-ray2.20A40-400[»]
DisProtiDP00351.
ProteinModelPortaliQ27974.
SMRiQ27974.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ27974.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati33 – 3614
Repeati37 – 4024
Repeati41 – 4434
Domaini52 – 219Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST168
Domaini225 – 363C2 tensin-typePROSITE-ProRule annotationAdd BLAST139
Domaini846 – 910JPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 443 X 4 AA approximate tandem repeatsAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi406 – 414SH3-bindingSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi463 – 757Pro-richAdd BLAST295
Compositional biasi526 – 529Poly-Gly4

Sequence similaritiesi

Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiKOG0431. Eukaryota.
KOG2283. Eukaryota.
ENOG41129H2. LUCA.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ27974.
KOiK09526.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM01326. PTEN_C2. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSGASSPD MEPSYGGGLF DMVKGGAGRL FSNLKDNLKD TLKDTSSRVI
60 70 80 90 100
QSVTSYTKGD LDFTYVTSRI IVMSFPLDSV DIGFRNQVDD IRSFLDSRHL
110 120 130 140 150
DHYTVYNLSP KSYRTAKFHS RVSECSWPIR QAPSLHNLFA VCRNMYNWLL
160 170 180 190 200
QNPKNVCVVH CLDGRAASSI LVGAMFIFCN LYSTPGPAVR LLYAKRPGIG
210 220 230 240 250
LSPSHRRYLG YMCDLLADKP YRPHFKPLTI KSITVSPVPF FNKQRNGCRP
260 270 280 290 300
YCDVLIGETK IYTTCADFER MKEYRVQDGK IFIPLSITVQ GDVVVSMYHL
310 320 330 340 350
RSTIGSRLQA KVTNTQIFQL QFHTGFIPLD TTVLKFTKPE LDACDVPEKY
360 370 380 390 400
PQLFQVTLDV ELQPHDKVME LTPPWEHYCT KDVNPSILFS SHQEHQDTLV
410 420 430 440 450
LGGQAPIDIP PDNPRHFGQG GFFSTLCWQD QKSEKSFCEE DHAALVNQES
460 470 480 490 500
EQSDDELLTL SSPHGNANGD KPHAARKPSK KQQEPAAPAP PEDVDLLGLE
510 520 530 540 550
GSAVSKNFSS PAAPPSNSEL LSDLFGGGGA AGPVQSGQSG VDDVFHPSGP
560 570 580 590 600
TSTQSTPRRS ATSTSASPTL RVGEGATFDP FGAPSKPSGQ DLLGSFLNTA
610 620 630 640 650
SASSDPFLQP TRSPSPTVHA SSTPAVNIQP DVSGAWDWHT KPGGFGMGSK
660 670 680 690 700
SAATSPTGSS HGTPTHQNKP QTLDPFADLG TLGGSSFASK PSTPTGLGGG
710 720 730 740 750
FPPLSSPQKA SPQPMGGGWQ QGGGYNWQQT QSKPQSSMPH SSPQNRPNYN
760 770 780 790 800
VSFSSMPGGQ NERGKAAANL EGKQKAADFE DLLSGQGFNA HKDKKGPRTI
810 820 830 840 850
AEMRKEEMAK EMDPEKLKIL EWIEGKERNI RALLSTMHTV LWAGETKWKP
860 870 880 890 900
VGMADLVTPE QVKKVYRKAV LVVHPDKATG QPYEQYAKMI FMELNDAWSE
910
FENQGQKPLY
Length:910
Mass (Da):99,512
Last modified:November 1, 1997 - v1
Checksum:iBC156DC1CF3487FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09237 mRNA. Translation: AAA79037.1.
PIRiS68983.
RefSeqiNP_777261.1. NM_174836.2.
UniGeneiBt.111572.

Genome annotation databases

GeneIDi317659.
KEGGibta:317659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09237 mRNA. Translation: AAA79037.1.
PIRiS68983.
RefSeqiNP_777261.1. NM_174836.2.
UniGeneiBt.111572.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4CNMR-A737-910[»]
1NZ6X-ray2.50A/B810-910[»]
1Q2Gmodel-A776-910[»]
1XI5electron microscopy12.00J/K/L/M/N/O/P/Q/R797-910[»]
2QWNX-ray2.40B812-905[»]
2QWOX-ray1.70B813-904[»]
2QWPX-ray1.75B813-904[»]
2QWQX-ray2.21B813-904[»]
2QWRX-ray2.21B813-904[»]
3N0AX-ray2.20A40-400[»]
DisProtiDP00351.
ProteinModelPortaliQ27974.
SMRiQ27974.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41952N.
MINTiMINT-205318.
STRINGi9913.ENSBTAP00000041032.

Proteomic databases

PaxDbiQ27974.
PRIDEiQ27974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi317659.
KEGGibta:317659.

Organism-specific databases

CTDi9829.

Phylogenomic databases

eggNOGiKOG0431. Eukaryota.
KOG2283. Eukaryota.
ENOG41129H2. LUCA.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ27974.
KOiK09526.

Miscellaneous databases

EvolutionaryTraceiQ27974.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM01326. PTEN_C2. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAUXI_BOVIN
AccessioniPrimary (citable) accession number: Q27974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.