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Q27971

- CAN2_BOVIN

UniProt

Q27971 - CAN2_BOVIN

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Protein
Calpain-2 catalytic subunit
Gene
CAPN2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions By similarity.

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Calcium 3; via carbonyl oxygen By similarity
Metal bindingi96 – 961Calcium 3 By similarity
Active sitei105 – 1051 By similarity
Metal bindingi175 – 1751Calcium 3 By similarity
Metal bindingi229 – 2291Calcium 2 By similarity
Metal bindingi230 – 2301Calcium 2 By similarity
Active sitei262 – 2621 By similarity
Active sitei286 – 2861 By similarity
Metal bindingi292 – 2921Calcium 4 By similarity
Metal bindingi299 – 2991Calcium 4 By similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygen By similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygen By similarity
Metal bindingi545 – 5451Calcium 5 By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygen By similarity
Metal bindingi552 – 5521Calcium 5 By similarity
Metal bindingi585 – 5851Calcium 6 By similarity
Metal bindingi587 – 5871Calcium 6 By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygen By similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygen By similarity
Metal bindingi596 – 5961Calcium 6 By similarity
Metal bindingi615 – 6151Calcium 7 By similarity
Metal bindingi617 – 6171Calcium 7 By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygen By similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygen By similarity
Metal bindingi626 – 6261Calcium 7 By similarity
Metal bindingi658 – 6581Calcium 1 By similarity
Metal bindingi661 – 6611Calcium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121 By similarity
Add
BLAST
Calcium bindingi615 – 626122 By similarity
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. blastocyst development Source: Ensembl
  2. cellular response to amino acid stimulus Source: UniProtKB
  3. myoblast fusion Source: Ensembl
  4. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 16

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

GO - Cellular componenti

  1. chromatin Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. dendrite Source: UniProtKB
  4. nucleus Source: Ensembl
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Propeptidei2 – 1918Anchors to the small subunit Reviewed prediction
PRO_0000317423Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunit
PRO_0000207702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ27971.
PRIDEiQ27971.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Protein-protein interaction databases

IntActiQ27971. 1 interaction.
MINTiMINT-6744807.

Structurei

3D structure databases

ProteinModelPortaliQ27971.
SMRiQ27971. Positions 2-700.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalytic
Add
BLAST
Domaini572 – 60534EF-hand 1
Add
BLAST
Domaini602 – 63736EF-hand 2
Add
BLAST
Domaini667 – 70034EF-hand 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain III By similarity
Add
BLAST
Regioni515 – 52915Linker By similarity
Add
BLAST
Regioni530 – 700171Domain IV By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 3 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00750000117643.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27971.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG7RV9FM.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27971-1 [UniParc]FASTAAdd to Basket

« Hide

MAGIAAKLAK DREAAEGLGS HERAVKYLNQ DYAALRDECL EAGALFQDPS    50
FPALPSSLGF KELGPYSSKT RGIEWKRPTE ICDNPQFITG GATRTDICQG 100
ALGDCWLLAA IASLTLNEEI LARVVPLDQS FQENYAGIFH FQFWQYGEWV 150
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT 200
TEGFEDFTGG IAEWYELRKA PPNLFRIIQK ALQKGSLLGC SIDITSAADS 250
EAITFQKLVK GHAYSVTGAE EVESRGSLQK LIRIRNPWGE VEWTGQWNDN 300
CPNWNTVDPE VRETLTRQHE DGEFWMSFND FLRHYSRLEI CNLTPDTLTS 350
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDQED 400
GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLSK 450
KFFLTTRARE RSDTFINLRE VLNRFKLPPG EYIVVPSTFE PNKDGDFCIR 500
VFSEKKADYQ VVDDEIEANI DEIDISEDDI DDGFRRLFAQ LAGEDAEISA 550
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL 600
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF 650
ADDDLIIDFD NFVRCLIRLE TLFRIFKQLD PENTGMIQLD LISWLSFSVL 700
Length:700
Mass (Da):79,935
Last modified:February 5, 2008 - v2
Checksum:i00FD932E9C6AB268
GO

Sequence cautioni

The sequence AAA18455.1 differs from that shown. Reason: Frameshift at positions 470 and 480.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti417 – 4171R → Q in AAA18455. 1 Publication
Sequence conflicti460 – 4623ERS → DV in AAA18455. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC134526 mRNA. Translation: AAI34527.1.
U07850 mRNA. Translation: AAA18455.1. Frameshift.
RefSeqiNP_001096556.1. NM_001103086.1.
UniGeneiBt.60825.

Genome annotation databases

EnsembliENSBTAT00000047532; ENSBTAP00000044733; ENSBTAG00000012778.
GeneIDi281662.
KEGGibta:281662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC134526 mRNA. Translation: AAI34527.1 .
U07850 mRNA. Translation: AAA18455.1 . Frameshift.
RefSeqi NP_001096556.1. NM_001103086.1.
UniGenei Bt.60825.

3D structure databases

ProteinModelPortali Q27971.
SMRi Q27971. Positions 2-700.
ModBasei Search...

Protein-protein interaction databases

IntActi Q27971. 1 interaction.
MINTi MINT-6744807.

Chemistry

BindingDBi Q27971.
ChEMBLi CHEMBL3612.

Protein family/group databases

MEROPSi C02.002.

Proteomic databases

PaxDbi Q27971.
PRIDEi Q27971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000047532 ; ENSBTAP00000044733 ; ENSBTAG00000012778 .
GeneIDi 281662.
KEGGi bta:281662.

Organism-specific databases

CTDi 824.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00750000117643.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi Q27971.
KOi K03853.
OMAi DTYKKWK.
OrthoDBi EOG7RV9FM.
TreeFami TF314748.

Miscellaneous databases

NextBioi 20805593.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  2. "Cloning the partial cDNA's for the calpain family of protease genes from bovine muscle."
    Sun W., Bidwell C.A., Ji S., Hancock D.L.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 377-585.
    Tissue: Skeletal muscle.

Entry informationi

Entry nameiCAN2_BOVIN
AccessioniPrimary (citable) accession number: Q27971
Secondary accession number(s): A4IFD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi