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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi91Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi96Calcium 3By similarity1
Active sitei105By similarity1
Metal bindingi175Calcium 3By similarity1
Metal bindingi229Calcium 2By similarity1
Metal bindingi230Calcium 2By similarity1
Active sitei262By similarity1
Active sitei286By similarity1
Metal bindingi292Calcium 4By similarity1
Metal bindingi299Calcium 4By similarity1
Metal bindingi323Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi542Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi545Calcium 5By similarity1
Metal bindingi547Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi552Calcium 5By similarity1
Metal bindingi585Calcium 6By similarity1
Metal bindingi587Calcium 6By similarity1
Metal bindingi589Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi591Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi596Calcium 6By similarity1
Metal bindingi615Calcium 7By similarity1
Metal bindingi617Calcium 7By similarity1
Metal bindingi619Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi621Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi626Calcium 7By similarity1
Metal bindingi658Calcium 1By similarity1
Metal bindingi661Calcium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi585 – 5961PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi615 – 6262PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3612.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00003174232 – 19Anchors to the small subunitSequence analysisAdd BLAST18
ChainiPRO_000020770220 – 700Calpain-2 catalytic subunitAdd BLAST681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ27971.
PeptideAtlasiQ27971.
PRIDEiQ27971.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSBTAG00000012778.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Interacts with CPEB3; this leads to cleavage of CPEB3.By similarity

Protein-protein interaction databases

IntActiQ27971. 1 interactor.
MINTiMINT-6744807.
STRINGi9913.ENSBTAP00000044733.

Chemistry databases

BindingDBiQ27971.

Structurei

3D structure databases

ProteinModelPortaliQ27971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 344Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini572 – 605EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini602 – 637EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini667 – 700EF-hand 3PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 514Domain IIIBy similarityAdd BLAST170
Regioni515 – 529LinkerBy similarityAdd BLAST15
Regioni530 – 700Domain IVBy similarityAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27971.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG091G049E.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIAAKLAK DREAAEGLGS HERAVKYLNQ DYAALRDECL EAGALFQDPS
60 70 80 90 100
FPALPSSLGF KELGPYSSKT RGIEWKRPTE ICDNPQFITG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPLDQS FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT
210 220 230 240 250
TEGFEDFTGG IAEWYELRKA PPNLFRIIQK ALQKGSLLGC SIDITSAADS
260 270 280 290 300
EAITFQKLVK GHAYSVTGAE EVESRGSLQK LIRIRNPWGE VEWTGQWNDN
310 320 330 340 350
CPNWNTVDPE VRETLTRQHE DGEFWMSFND FLRHYSRLEI CNLTPDTLTS
360 370 380 390 400
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDQED
410 420 430 440 450
GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLSK
460 470 480 490 500
KFFLTTRARE RSDTFINLRE VLNRFKLPPG EYIVVPSTFE PNKDGDFCIR
510 520 530 540 550
VFSEKKADYQ VVDDEIEANI DEIDISEDDI DDGFRRLFAQ LAGEDAEISA
560 570 580 590 600
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF
660 670 680 690 700
ADDDLIIDFD NFVRCLIRLE TLFRIFKQLD PENTGMIQLD LISWLSFSVL
Length:700
Mass (Da):79,935
Last modified:February 5, 2008 - v2
Checksum:i00FD932E9C6AB268
GO

Sequence cautioni

The sequence AAA18455 differs from that shown. Reason: Frameshift at positions 470 and 480.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti417R → Q in AAA18455 (Ref. 2) Curated1
Sequence conflicti460 – 462ERS → DV in AAA18455 (Ref. 2) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134526 mRNA. Translation: AAI34527.1.
U07850 mRNA. Translation: AAA18455.1. Frameshift.
RefSeqiNP_001096556.1. NM_001103086.1.
UniGeneiBt.60825.

Genome annotation databases

EnsembliENSBTAT00000047532; ENSBTAP00000044733; ENSBTAG00000012778.
GeneIDi281662.
KEGGibta:281662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134526 mRNA. Translation: AAI34527.1.
U07850 mRNA. Translation: AAA18455.1. Frameshift.
RefSeqiNP_001096556.1. NM_001103086.1.
UniGeneiBt.60825.

3D structure databases

ProteinModelPortaliQ27971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ27971. 1 interactor.
MINTiMINT-6744807.
STRINGi9913.ENSBTAP00000044733.

Chemistry databases

BindingDBiQ27971.
ChEMBLiCHEMBL3612.

Protein family/group databases

MEROPSiC02.002.

Proteomic databases

PaxDbiQ27971.
PeptideAtlasiQ27971.
PRIDEiQ27971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000047532; ENSBTAP00000044733; ENSBTAG00000012778.
GeneIDi281662.
KEGGibta:281662.

Organism-specific databases

CTDi824.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27971.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG091G049E.
TreeFamiTF314748.

Enzyme and pathway databases

ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.

Gene expression databases

BgeeiENSBTAG00000012778.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN2_BOVIN
AccessioniPrimary (citable) accession number: Q27971
Secondary accession number(s): A4IFD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.