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Q27971

- CAN2_BOVIN

UniProt

Q27971 - CAN2_BOVIN

Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 7 calcium ions.By similarity

    Enzyme regulationi

    Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi96 – 961Calcium 3By similarity
    Active sitei105 – 1051By similarity
    Metal bindingi175 – 1751Calcium 3By similarity
    Metal bindingi229 – 2291Calcium 2By similarity
    Metal bindingi230 – 2301Calcium 2By similarity
    Active sitei262 – 2621By similarity
    Active sitei286 – 2861By similarity
    Metal bindingi292 – 2921Calcium 4By similarity
    Metal bindingi299 – 2991Calcium 4By similarity
    Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi545 – 5451Calcium 5By similarity
    Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi552 – 5521Calcium 5By similarity
    Metal bindingi585 – 5851Calcium 6By similarity
    Metal bindingi587 – 5871Calcium 6By similarity
    Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi596 – 5961Calcium 6By similarity
    Metal bindingi615 – 6151Calcium 7By similarity
    Metal bindingi617 – 6171Calcium 7By similarity
    Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi626 – 6261Calcium 7By similarity
    Metal bindingi658 – 6581Calcium 1By similarity
    Metal bindingi661 – 6611Calcium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi585 – 596121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi615 – 626122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. blastocyst development Source: Ensembl
    2. cellular response to amino acid stimulus Source: UniProtKB
    3. myoblast fusion Source: Ensembl
    4. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiC02.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-2 catalytic subunit (EC:3.4.22.53)
    Alternative name(s):
    Calcium-activated neutral proteinase 2
    Short name:
    CANP 2
    Calpain M-type
    Calpain-2 large subunit
    Millimolar-calpain
    Short name:
    M-calpain
    Gene namesi
    Name:CAPN2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. chromatin Source: Ensembl
    2. cytoplasm Source: UniProtKB
    3. dendrite Source: UniProtKB
    4. nucleus Source: Ensembl
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000317423Add
    BLAST
    Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000207702Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ27971.
    PRIDEiQ27971.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Protein-protein interaction databases

    IntActiQ27971. 1 interaction.
    MINTiMINT-6744807.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27971.
    SMRiQ27971. Positions 2-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini667 – 70034EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni345 – 514170Domain IIIBy similarityAdd
    BLAST
    Regioni515 – 52915LinkerBy similarityAdd
    BLAST
    Regioni530 – 700171Domain IVBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    GeneTreeiENSGT00750000117643.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiQ27971.
    KOiK03853.
    OMAiDTYKKWK.
    OrthoDBiEOG7RV9FM.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27971-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGIAAKLAK DREAAEGLGS HERAVKYLNQ DYAALRDECL EAGALFQDPS    50
    FPALPSSLGF KELGPYSSKT RGIEWKRPTE ICDNPQFITG GATRTDICQG 100
    ALGDCWLLAA IASLTLNEEI LARVVPLDQS FQENYAGIFH FQFWQYGEWV 150
    EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT 200
    TEGFEDFTGG IAEWYELRKA PPNLFRIIQK ALQKGSLLGC SIDITSAADS 250
    EAITFQKLVK GHAYSVTGAE EVESRGSLQK LIRIRNPWGE VEWTGQWNDN 300
    CPNWNTVDPE VRETLTRQHE DGEFWMSFND FLRHYSRLEI CNLTPDTLTS 350
    DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDQED 400
    GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLSK 450
    KFFLTTRARE RSDTFINLRE VLNRFKLPPG EYIVVPSTFE PNKDGDFCIR 500
    VFSEKKADYQ VVDDEIEANI DEIDISEDDI DDGFRRLFAQ LAGEDAEISA 550
    FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL 600
    WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF 650
    ADDDLIIDFD NFVRCLIRLE TLFRIFKQLD PENTGMIQLD LISWLSFSVL 700
    Length:700
    Mass (Da):79,935
    Last modified:February 5, 2008 - v2
    Checksum:i00FD932E9C6AB268
    GO

    Sequence cautioni

    The sequence AAA18455.1 differs from that shown. Reason: Frameshift at positions 470 and 480.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti417 – 4171R → Q in AAA18455. 1 PublicationCurated
    Sequence conflicti460 – 4623ERS → DV in AAA18455. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC134526 mRNA. Translation: AAI34527.1.
    U07850 mRNA. Translation: AAA18455.1. Frameshift.
    RefSeqiNP_001096556.1. NM_001103086.1.
    UniGeneiBt.60825.

    Genome annotation databases

    EnsembliENSBTAT00000047532; ENSBTAP00000044733; ENSBTAG00000012778.
    GeneIDi281662.
    KEGGibta:281662.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC134526 mRNA. Translation: AAI34527.1 .
    U07850 mRNA. Translation: AAA18455.1 . Frameshift.
    RefSeqi NP_001096556.1. NM_001103086.1.
    UniGenei Bt.60825.

    3D structure databases

    ProteinModelPortali Q27971.
    SMRi Q27971. Positions 2-700.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q27971. 1 interaction.
    MINTi MINT-6744807.

    Chemistry

    BindingDBi Q27971.
    ChEMBLi CHEMBL3612.

    Protein family/group databases

    MEROPSi C02.002.

    Proteomic databases

    PaxDbi Q27971.
    PRIDEi Q27971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000047532 ; ENSBTAP00000044733 ; ENSBTAG00000012778 .
    GeneIDi 281662.
    KEGGi bta:281662.

    Organism-specific databases

    CTDi 824.

    Phylogenomic databases

    eggNOGi NOG327523.
    GeneTreei ENSGT00750000117643.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi Q27971.
    KOi K03853.
    OMAi DTYKKWK.
    OrthoDBi EOG7RV9FM.
    TreeFami TF314748.

    Miscellaneous databases

    NextBioi 20805593.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.
    2. "Cloning the partial cDNA's for the calpain family of protease genes from bovine muscle."
      Sun W., Bidwell C.A., Ji S., Hancock D.L.
      Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 377-585.
      Tissue: Skeletal muscle.

    Entry informationi

    Entry nameiCAN2_BOVIN
    AccessioniPrimary (citable) accession number: Q27971
    Secondary accession number(s): A4IFD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3