Reviewed, UniProtKB/Swiss-Prot Q27971 (CAN2_BOVIN)

Names and origin

Protein names

Recommended name:
    Calpain-2 catalytic subunit
    EC=3.4.22.53
Alternative name(s):
    Calpain-2 large subunit
    Calcium-activated neutral proteinase 2
      Short name=CANP 2
    Calpain M-type
    M-calpain
    Millimolar-calpain

Gene names

Name:

CAPN2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction By similarity.
Catalytic activity	Broad endopeptidase specificity.
Cofactor	Binds 3 calcium ions.
Enzyme regulation	Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.
Subunit structure	Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 3 EF-hand domains.
Sequence caution	The sequence AAA18455.1 differs from that shown. Reason: Frameshift at positions 470 and 480.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Propeptide	2 – 19	18	Anchors to the small subunit Potential		PRO_0000317423
	Chain	20 – 700	681	Calpain-2 catalytic subunit		PRO_0000207702
Regions
	Domain	45 – 344	300	Calpain catalytic
	Domain	572 – 605	34	EF-hand 1
	Domain	602 – 637	36	EF-hand 2
	Domain	667 – 700	34	EF-hand 3
	Calcium binding	585 – 596	12	1 By similarity
	Calcium binding	615 – 626	12	2 By similarity
	Region	345 – 514	170	Domain III By similarity
	Region	515 – 529	15	Linker By similarity
	Region	530 – 700	171	Domain IV By similarity
Sites
	Active site	105	1	By similarity
	Active site	262	1	By similarity
	Active site	286	1	By similarity
Experimental info
	Sequence conflict	417	1	R → Q in AAA18455. Ref.2
	Sequence conflict	460 – 462	3	ERS → DV in AAA18455. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q27971-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 00FD932E9C6AB268	FASTA	700	79,935
	10 20 30 40 50 60 MAGIAAKLAK DREAAEGLGS HERAVKYLNQ DYAALRDECL EAGALFQDPS FPALPSSLGF 70 80 90 100 110 120 KELGPYSSKT RGIEWKRPTE ICDNPQFITG GATRTDICQG ALGDCWLLAA IASLTLNEEI 130 140 150 160 170 180 LARVVPLDQS FQENYAGIFH FQFWQYGEWV EVVVDDRLPT KDGELLFVHS AEGSEFWSAL 190 200 210 220 230 240 LEKAYAKING CYEALSGGAT TEGFEDFTGG IAEWYELRKA PPNLFRIIQK ALQKGSLLGC 250 260 270 280 290 300 SIDITSAADS EAITFQKLVK GHAYSVTGAE EVESRGSLQK LIRIRNPWGE VEWTGQWNDN 310 320 330 340 350 360 CPNWNTVDPE VRETLTRQHE DGEFWMSFND FLRHYSRLEI CNLTPDTLTS DSYKKWKLTK 370 380 390 400 410 420 MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDQED GESGCTFLVG LIQKHRRRQR 430 440 450 460 470 480 KMGEDMHTIG FGIYEVPEEL TGQTNIHLSK KFFLTTRARE RSDTFINLRE VLNRFKLPPG 490 500 510 520 530 540 EYIVVPSTFE PNKDGDFCIR VFSEKKADYQ VVDDEIEANI DEIDISEDDI DDGFRRLFAQ 550 560 570 580 590 600 LAGEDAEISA FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL 610 620 630 640 650 660 WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF ADDDLIIDFD 670 680 690 700 NFVRCLIRLE TLFRIFKQLD PENTGMIQLD LISWLSFSVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.
[2]	"Cloning the partial cDNA's for the calpain family of protease genes from bovine muscle." Sun W., Bidwell C.A., Ji S., Hancock D.L. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 377-585. Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC134526 mRNA. Translation: AAI34527.1. U07850 mRNA. Translation: AAA18455.1. Frameshift.
IPI	IPI00695673.
RefSeq	NP_001096556.1.
UniGene	Bt.60825
3D structure databases
HSSP	HSSP built from PDB template 1KFX based on UniProtKB P17655.
ModBase	Search...
Protein family/group databases
MEROPS	C02.002.
Genome annotation databases
Ensembl	ENSBTAG00000012778. Bos taurus. [Contig view]
GeneID	281662.
KEGG	bta:281662.
Phylogenomic databases
HOVERGEN	Q27971.
OMA	Q27971. LTKMDGN.
Enzyme and pathway databases
BRENDA	3.4.22.53. 251.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018247. EF_HAND_1. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50203. CALPAIN_CAT. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 3 hits. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAN2_BOVIN

Accession

Primary (citable) accession number: Q27971
Secondary accession number(s): A4IFD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)