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Q27971

- CAN2_BOVIN

UniProt

Q27971 - CAN2_BOVIN

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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca(2+) ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi96 – 961Calcium 3By similarity
Active sitei105 – 1051By similarity
Metal bindingi175 – 1751Calcium 3By similarity
Metal bindingi229 – 2291Calcium 2By similarity
Metal bindingi230 – 2301Calcium 2By similarity
Active sitei262 – 2621By similarity
Active sitei286 – 2861By similarity
Metal bindingi292 – 2921Calcium 4By similarity
Metal bindingi299 – 2991Calcium 4By similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi545 – 5451Calcium 5By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi552 – 5521Calcium 5By similarity
Metal bindingi585 – 5851Calcium 6By similarity
Metal bindingi587 – 5871Calcium 6By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi596 – 5961Calcium 6By similarity
Metal bindingi615 – 6151Calcium 7By similarity
Metal bindingi617 – 6171Calcium 7By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi626 – 6261Calcium 7By similarity
Metal bindingi658 – 6581Calcium 1By similarity
Metal bindingi661 – 6611Calcium 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi615 – 626122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. blastocyst development Source: Ensembl
  2. cellular response to amino acid stimulus Source: UniProtKB
  3. myoblast fusion Source: Ensembl
  4. proteolysis Source: UniProtKB
  5. regulation of cytoskeleton organization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 16

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. chromatin Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. cytosol Source: Ensembl
  4. dendrite Source: UniProtKB
  5. extracellular vesicular exosome Source: Ensembl
  6. Golgi apparatus Source: Ensembl
  7. membrane raft Source: Ensembl
  8. nucleus Source: Ensembl
  9. perinuclear endoplasmic reticulum Source: Ensembl
  10. plasma membrane Source: UniProtKB-KW
  11. pseudopodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000317423Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000207702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ27971.
PRIDEiQ27971.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Protein-protein interaction databases

IntActiQ27971. 1 interaction.
MINTiMINT-6744807.

Structurei

3D structure databases

ProteinModelPortaliQ27971.
SMRiQ27971. Positions 2-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini667 – 70034EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain IIIBy similarityAdd
BLAST
Regioni515 – 52915LinkerBy similarityAdd
BLAST
Regioni530 – 700171Domain IVBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27971.
KOiK03853.
OMAiDTYKKWK.
OrthoDBiEOG7RV9FM.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27971-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGIAAKLAK DREAAEGLGS HERAVKYLNQ DYAALRDECL EAGALFQDPS
60 70 80 90 100
FPALPSSLGF KELGPYSSKT RGIEWKRPTE ICDNPQFITG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPLDQS FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT
210 220 230 240 250
TEGFEDFTGG IAEWYELRKA PPNLFRIIQK ALQKGSLLGC SIDITSAADS
260 270 280 290 300
EAITFQKLVK GHAYSVTGAE EVESRGSLQK LIRIRNPWGE VEWTGQWNDN
310 320 330 340 350
CPNWNTVDPE VRETLTRQHE DGEFWMSFND FLRHYSRLEI CNLTPDTLTS
360 370 380 390 400
DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDQED
410 420 430 440 450
GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLSK
460 470 480 490 500
KFFLTTRARE RSDTFINLRE VLNRFKLPPG EYIVVPSTFE PNKDGDFCIR
510 520 530 540 550
VFSEKKADYQ VVDDEIEANI DEIDISEDDI DDGFRRLFAQ LAGEDAEISA
560 570 580 590 600
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF
660 670 680 690 700
ADDDLIIDFD NFVRCLIRLE TLFRIFKQLD PENTGMIQLD LISWLSFSVL
Length:700
Mass (Da):79,935
Last modified:February 5, 2008 - v2
Checksum:i00FD932E9C6AB268
GO

Sequence cautioni

The sequence AAA18455.1 differs from that shown. Reason: Frameshift at positions 470 and 480. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti417 – 4171R → Q in AAA18455. 1 PublicationCurated
Sequence conflicti460 – 4623ERS → DV in AAA18455. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134526 mRNA. Translation: AAI34527.1.
U07850 mRNA. Translation: AAA18455.1. Frameshift.
RefSeqiNP_001096556.1. NM_001103086.1.
UniGeneiBt.60825.

Genome annotation databases

EnsembliENSBTAT00000047532; ENSBTAP00000044733; ENSBTAG00000012778.
GeneIDi281662.
KEGGibta:281662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134526 mRNA. Translation: AAI34527.1 .
U07850 mRNA. Translation: AAA18455.1 . Frameshift.
RefSeqi NP_001096556.1. NM_001103086.1.
UniGenei Bt.60825.

3D structure databases

ProteinModelPortali Q27971.
SMRi Q27971. Positions 2-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q27971. 1 interaction.
MINTi MINT-6744807.

Chemistry

BindingDBi Q27971.
ChEMBLi CHEMBL3612.

Protein family/group databases

MEROPSi C02.002.

Proteomic databases

PaxDbi Q27971.
PRIDEi Q27971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000047532 ; ENSBTAP00000044733 ; ENSBTAG00000012778 .
GeneIDi 281662.
KEGGi bta:281662.

Organism-specific databases

CTDi 824.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00760000118971.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi Q27971.
KOi K03853.
OMAi DTYKKWK.
OrthoDBi EOG7RV9FM.
TreeFami TF314748.

Miscellaneous databases

NextBioi 20805593.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  2. "Cloning the partial cDNA's for the calpain family of protease genes from bovine muscle."
    Sun W., Bidwell C.A., Ji S., Hancock D.L.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 377-585.
    Tissue: Skeletal muscle.

Entry informationi

Entry nameiCAN2_BOVIN
AccessioniPrimary (citable) accession number: Q27971
Secondary accession number(s): A4IFD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: November 26, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3