Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q27971 (CAN2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calpain-2 catalytic subunit

EC=3.4.22.53
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name=CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name=M-calpain
Gene names
Name:CAPN2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length700 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.

Catalytic activity

Broad endopeptidase specificity.

Cofactor

Binds 7 calcium ions By similarity.

Enzyme regulation

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Subunit structure

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Subcellular location

Cytoplasm By similarity. Cell membrane By similarity. Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the peptidase C2 family.

Contains 1 calpain catalytic domain.

Contains 3 EF-hand domains.

Sequence caution

The sequence AAA18455.1 differs from that shown. Reason: Frameshift at positions 470 and 480.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Propeptide2 – 1918Anchors to the small subunit Potential
PRO_0000317423
Chain20 – 700681Calpain-2 catalytic subunit
PRO_0000207702

Regions

Domain45 – 344300Calpain catalytic
Domain572 – 60534EF-hand 1
Domain602 – 63736EF-hand 2
Domain667 – 70034EF-hand 3
Calcium binding585 – 596121 By similarity
Calcium binding615 – 626122 By similarity
Region345 – 514170Domain III By similarity
Region515 – 52915Linker By similarity
Region530 – 700171Domain IV By similarity

Sites

Active site1051 By similarity
Active site2621 By similarity
Active site2861 By similarity
Metal binding911Calcium 3; via carbonyl oxygen By similarity
Metal binding961Calcium 3 By similarity
Metal binding1751Calcium 3 By similarity
Metal binding2291Calcium 2 By similarity
Metal binding2301Calcium 2 By similarity
Metal binding2921Calcium 4 By similarity
Metal binding2991Calcium 4 By similarity
Metal binding3231Calcium 4; via carbonyl oxygen By similarity
Metal binding5421Calcium 5; via carbonyl oxygen By similarity
Metal binding5451Calcium 5 By similarity
Metal binding5471Calcium 5; via carbonyl oxygen By similarity
Metal binding5521Calcium 5 By similarity
Metal binding5851Calcium 6 By similarity
Metal binding5871Calcium 6 By similarity
Metal binding5891Calcium 6; via carbonyl oxygen By similarity
Metal binding5911Calcium 6; via carbonyl oxygen By similarity
Metal binding5961Calcium 6 By similarity
Metal binding6151Calcium 7 By similarity
Metal binding6171Calcium 7 By similarity
Metal binding6191Calcium 7; via carbonyl oxygen By similarity
Metal binding6211Calcium 7; via carbonyl oxygen By similarity
Metal binding6261Calcium 7 By similarity
Metal binding6581Calcium 1 By similarity
Metal binding6611Calcium 1 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Experimental info

Sequence conflict4171R → Q in AAA18455. Ref.2
Sequence conflict460 – 4623ERS → DV in AAA18455. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q27971 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: 00FD932E9C6AB268

FASTA70079,935
        10         20         30         40         50         60 
MAGIAAKLAK DREAAEGLGS HERAVKYLNQ DYAALRDECL EAGALFQDPS FPALPSSLGF 

        70         80         90        100        110        120 
KELGPYSSKT RGIEWKRPTE ICDNPQFITG GATRTDICQG ALGDCWLLAA IASLTLNEEI 

       130        140        150        160        170        180 
LARVVPLDQS FQENYAGIFH FQFWQYGEWV EVVVDDRLPT KDGELLFVHS AEGSEFWSAL 

       190        200        210        220        230        240 
LEKAYAKING CYEALSGGAT TEGFEDFTGG IAEWYELRKA PPNLFRIIQK ALQKGSLLGC 

       250        260        270        280        290        300 
SIDITSAADS EAITFQKLVK GHAYSVTGAE EVESRGSLQK LIRIRNPWGE VEWTGQWNDN 

       310        320        330        340        350        360 
CPNWNTVDPE VRETLTRQHE DGEFWMSFND FLRHYSRLEI CNLTPDTLTS DSYKKWKLTK 

       370        380        390        400        410        420 
MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDQED GESGCTFLVG LIQKHRRRQR 

       430        440        450        460        470        480 
KMGEDMHTIG FGIYEVPEEL TGQTNIHLSK KFFLTTRARE RSDTFINLRE VLNRFKLPPG 

       490        500        510        520        530        540 
EYIVVPSTFE PNKDGDFCIR VFSEKKADYQ VVDDEIEANI DEIDISEDDI DDGFRRLFAQ 

       550        560        570        580        590        600 
LAGEDAEISA FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL 

       610        620        630        640        650        660 
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF ADDDLIIDFD 

       670        680        690        700 
NFVRCLIRLE TLFRIFKQLD PENTGMIQLD LISWLSFSVL 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.
[2]"Cloning the partial cDNA's for the calpain family of protease genes from bovine muscle."
Sun W., Bidwell C.A., Ji S., Hancock D.L.
Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 377-585.
Tissue: Skeletal muscle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC134526 mRNA. Translation: AAI34527.1.
U07850 mRNA. Translation: AAA18455.1. Frameshift.
RefSeqNP_001096556.1. NM_001103086.1.
UniGeneBt.60825.

3D structure databases

ProteinModelPortalQ27971.
SMRQ27971. Positions 2-700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ27971. 1 interaction.
MINTMINT-6744807.

Chemistry

BindingDBQ27971.
ChEMBLCHEMBL3612.

Protein family/group databases

MEROPSC02.002.

Proteomic databases

PaxDbQ27971.
PRIDEQ27971.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000047532; ENSBTAP00000044733; ENSBTAG00000012778.
GeneID281662.
KEGGbta:281662.

Organism-specific databases

CTD824.

Phylogenomic databases

eggNOGNOG327523.
GeneTreeENSGT00750000117643.
HOGENOMHOG000232035.
HOVERGENHBG012645.
InParanoidQ27971.
KOK03853.
OMADTYKKWK.
OrthoDBEOG7RV9FM.
TreeFamTF314748.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSPR00704. CALPAIN.
SMARTSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMSSF49758. SSF49758. 1 hit.
PROSITEPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805593.

Entry information

Entry nameCAN2_BOVIN
AccessionPrimary (citable) accession number: Q27971
Secondary accession number(s): A4IFD3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: May 14, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries