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Q27970

- CAN1_BOVIN

UniProt

Q27970 - CAN1_BOVIN

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Protein

Calpain-1 catalytic subunit

Gene

CAPN1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 4 Ca(2+) ions.By similarity

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 162Cleavage; for 78 kDa formBy similarity
Sitei27 – 282Cleavage; for 75 kDa formBy similarity
Active sitei115 – 1151By similarity
Active sitei272 – 2721By similarity
Active sitei296 – 2961By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi101 – 10881PROSITE-ProRule annotation
Calcium bindingi304 – 335322PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi600 – 611123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi630 – 641124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. proteolysis Source: UniProtKB
  2. receptor catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_201925. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiC02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase 1
Short name:
CANP 1
Calpain mu-type
Calpain-1 large subunit
Micromolar-calpain
Short name:
muCANP
Gene namesi
Name:CAPN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 29

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. cytosol Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 716715Calpain-1 catalytic subunitPRO_0000207693Add
BLAST

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiQ27970.
PRIDEiQ27970.

Expressioni

Gene expression databases

ExpressionAtlasiQ27970. baseline.

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) and a small (regulatory) subunit.

Protein-protein interaction databases

IntActiQ27970. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ27970.
SMRiQ27970. Positions 13-715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 354300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini543 – 57836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini587 – 62034EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini617 – 65236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini682 – 71635EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 528174Domain IIIAdd
BLAST
Regioni529 – 54416LinkerAdd
BLAST
Regioni545 – 715171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27970.
KOiK01367.
OMAiPQSLGYK.
OrthoDBiEOG7RV9FM.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27970-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEEFITPVY CTGVSAQVQK QRAKELGLGR HENAIKYLGQ DYEQLRVHCL
60 70 80 90 100
QRGALFRDEA FPPVPQSLGF KELGPNSSKT YGIKWKRPTE LFSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQDGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYNIILK ALERGSLLGC
260 270 280 290 300
SIDISSILDM EAVTFKKLVK GHAYSVTGAK QVNYQGQMVN LIRMRNPWGE
310 320 330 340 350
VEWTGAWSDG SSEWNGVDPY MREQLRVKME DGEFWMSFRD FMREFTRLEI
360 370 380 390 400
CNLTPDALKS QRFRNWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEETDDPDPD DYGGRESGCS FLLALMQKHR RRERRFGRDM ETIGFAVYEV
460 470 480 490 500
PPELMGQPAV HLKRDFFLSN ASRARSEQFI NLREVSTRFR LPPGEYVVVP
510 520 530 540 550
STFEPNKEGD FVLRFFSEKS AGTQELDDQV QANLPDEQVL SEEEIDENFK
560 570 580 590 600
SLFRQLAGED MEISVKELRT ILNRIISKHK DLRTTGFSLE SCRSMVNLMD
610 620 630 640 650
RDGNGKLGLV EFNILWNRIR NYLSIFRKFD LDKSGSMSAY EMRMAIEFAG
660 670 680 690 700
FKLNKKLYEL IITRYSEPDL AVDFDNFVCC LVRLETMFRF FKTLDTDLDG
710
VVTFDLFKWL QLTMFA
Length:716
Mass (Da):82,207
Last modified:December 12, 2006 - v3
Checksum:iA7F55C197BD5DF23
GO

Sequence cautioni

The sequence AAA18454.1 differs from that shown. Reason: Frameshift at positions 582 and 617. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211Q → T AA sequence (PubMed:2065086)Curated
Sequence conflicti316 – 3161G → A in AAF64504. (PubMed:11048924)Curated
Sequence conflicti381 – 3811A → V in AAF64504. (PubMed:11048924)Curated
Sequence conflicti530 – 5301V → I in AAA18454. 1 PublicationCurated
Sequence conflicti624 – 6241S → A in AAA18454. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248054, AF252504 Genomic DNA. Translation: AAF64504.2.
AF221129 mRNA. Translation: AAF32364.1.
BC123635 mRNA. Translation: AAI23636.1.
U07849 mRNA. Translation: AAA18454.1. Frameshift.
PIRiS16181.
RefSeqiNP_776684.1. NM_174259.2.
UniGeneiBt.252.

Genome annotation databases

EnsembliENSBTAT00000011678; ENSBTAP00000011678; ENSBTAG00000010230.
GeneIDi281661.
KEGGibta:281661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248054 , AF252504 Genomic DNA. Translation: AAF64504.2 .
AF221129 mRNA. Translation: AAF32364.1 .
BC123635 mRNA. Translation: AAI23636.1 .
U07849 mRNA. Translation: AAA18454.1 . Frameshift.
PIRi S16181.
RefSeqi NP_776684.1. NM_174259.2.
UniGenei Bt.252.

3D structure databases

ProteinModelPortali Q27970.
SMRi Q27970. Positions 13-715.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q27970. 1 interaction.

Protein family/group databases

MEROPSi C02.001.

Proteomic databases

PaxDbi Q27970.
PRIDEi Q27970.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000011678 ; ENSBTAP00000011678 ; ENSBTAG00000010230 .
GeneIDi 281661.
KEGGi bta:281661.

Organism-specific databases

CTDi 823.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00760000118971.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi Q27970.
KOi K01367.
OMAi PQSLGYK.
OrthoDBi EOG7RV9FM.
TreeFami TF314748.

Enzyme and pathway databases

Reactomei REACT_201925. Degradation of the extracellular matrix.

Miscellaneous databases

NextBioi 20805592.

Gene expression databases

ExpressionAtlasi Q27970. baseline.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF284. PTHR10183:SF284. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness."
    Smith T.P.L., Casas E., Rexroad C.E. III, Kappes S.M., Keele J.W.
    J. Anim. Sci. 78:2589-2594(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Bovine CAPN1 maps to a region containing a QTL for meat tenderness."
    Smith T.P.L., Casas E., Rexroad C.E., Kappes S.M., Keele J.W.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal muscle.
  4. Sun W., Bidwell C.A., Ji S., Hancock D.L.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 530-625.
    Tissue: Skeletal muscle.
  5. "Two-stage autolysis of the catalytic subunit initiates activation of calpain I."
    Zimmerman U.J., Schlaepfer W.W.
    Biochim. Biophys. Acta 1078:192-198(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-41.

Entry informationi

Entry nameiCAN1_BOVIN
AccessioniPrimary (citable) accession number: Q27970
Secondary accession number(s): Q9N0U3, Q9N0V6, Q9N185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 12, 2006
Last modified: November 26, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3