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Q27970

- CAN1_BOVIN

UniProt

Q27970 - CAN1_BOVIN

Protein

Calpain-1 catalytic subunit

Gene

CAPN1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 4 calcium ions.By similarity

    Enzyme regulationi

    Activated by micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei15 – 162Cleavage; for 78 kDa formBy similarity
    Sitei27 – 282Cleavage; for 75 kDa formBy similarity
    Active sitei115 – 1151By similarity
    Active sitei272 – 2721By similarity
    Active sitei296 – 2961By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi101 – 10881PROSITE-ProRule annotation
    Calcium bindingi304 – 335322PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi600 – 611123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi630 – 641124PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: UniProtKB
    2. receptor catabolic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_201925. Degradation of the extracellular matrix.

    Protein family/group databases

    MEROPSiC02.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-1 catalytic subunit (EC:3.4.22.52)
    Alternative name(s):
    Calcium-activated neutral proteinase 1
    Short name:
    CANP 1
    Calpain mu-type
    Calpain-1 large subunit
    Micromolar-calpain
    Short name:
    muCANP
    Gene namesi
    Name:CAPN1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 29

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 716715Calpain-1 catalytic subunitPRO_0000207693Add
    BLAST

    Post-translational modificationi

    Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms By similarity.By similarity

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    PaxDbiQ27970.
    PRIDEiQ27970.

    Interactioni

    Subunit structurei

    Heterodimer of a large (catalytic) and a small (regulatory) subunit.

    Protein-protein interaction databases

    IntActiQ27970. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27970.
    SMRiQ27970. Positions 13-715.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 354300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini543 – 57836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini587 – 62034EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini617 – 65236EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini682 – 71635EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 528174Domain IIIAdd
    BLAST
    Regioni529 – 54416LinkerAdd
    BLAST
    Regioni545 – 715171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    GeneTreeiENSGT00750000117643.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiQ27970.
    KOiK01367.
    OMAiPQSLGYK.
    OrthoDBiEOG7RV9FM.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PfamiPF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27970-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEEFITPVY CTGVSAQVQK QRAKELGLGR HENAIKYLGQ DYEQLRVHCL    50
    QRGALFRDEA FPPVPQSLGF KELGPNSSKT YGIKWKRPTE LFSNPQFIVD 100
    GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQDGYAGIFH 150
    FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG 200
    SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYNIILK ALERGSLLGC 250
    SIDISSILDM EAVTFKKLVK GHAYSVTGAK QVNYQGQMVN LIRMRNPWGE 300
    VEWTGAWSDG SSEWNGVDPY MREQLRVKME DGEFWMSFRD FMREFTRLEI 350
    CNLTPDALKS QRFRNWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR 400
    LEETDDPDPD DYGGRESGCS FLLALMQKHR RRERRFGRDM ETIGFAVYEV 450
    PPELMGQPAV HLKRDFFLSN ASRARSEQFI NLREVSTRFR LPPGEYVVVP 500
    STFEPNKEGD FVLRFFSEKS AGTQELDDQV QANLPDEQVL SEEEIDENFK 550
    SLFRQLAGED MEISVKELRT ILNRIISKHK DLRTTGFSLE SCRSMVNLMD 600
    RDGNGKLGLV EFNILWNRIR NYLSIFRKFD LDKSGSMSAY EMRMAIEFAG 650
    FKLNKKLYEL IITRYSEPDL AVDFDNFVCC LVRLETMFRF FKTLDTDLDG 700
    VVTFDLFKWL QLTMFA 716
    Length:716
    Mass (Da):82,207
    Last modified:December 12, 2006 - v3
    Checksum:iA7F55C197BD5DF23
    GO

    Sequence cautioni

    The sequence AAA18454.1 differs from that shown. Reason: Frameshift at positions 582 and 617.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti21 – 211Q → T AA sequence (PubMed:2065086)Curated
    Sequence conflicti316 – 3161G → A in AAF64504. (PubMed:11048924)Curated
    Sequence conflicti381 – 3811A → V in AAF64504. (PubMed:11048924)Curated
    Sequence conflicti530 – 5301V → I in AAA18454. 1 PublicationCurated
    Sequence conflicti624 – 6241S → A in AAA18454. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF248054, AF252504 Genomic DNA. Translation: AAF64504.2.
    AF221129 mRNA. Translation: AAF32364.1.
    BC123635 mRNA. Translation: AAI23636.1.
    U07849 mRNA. Translation: AAA18454.1. Frameshift.
    PIRiS16181.
    RefSeqiNP_776684.1. NM_174259.2.
    UniGeneiBt.252.

    Genome annotation databases

    EnsembliENSBTAT00000011678; ENSBTAP00000011678; ENSBTAG00000010230.
    GeneIDi281661.
    KEGGibta:281661.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF248054 , AF252504 Genomic DNA. Translation: AAF64504.2 .
    AF221129 mRNA. Translation: AAF32364.1 .
    BC123635 mRNA. Translation: AAI23636.1 .
    U07849 mRNA. Translation: AAA18454.1 . Frameshift.
    PIRi S16181.
    RefSeqi NP_776684.1. NM_174259.2.
    UniGenei Bt.252.

    3D structure databases

    ProteinModelPortali Q27970.
    SMRi Q27970. Positions 13-715.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q27970. 1 interaction.

    Protein family/group databases

    MEROPSi C02.001.

    Proteomic databases

    PaxDbi Q27970.
    PRIDEi Q27970.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000011678 ; ENSBTAP00000011678 ; ENSBTAG00000010230 .
    GeneIDi 281661.
    KEGGi bta:281661.

    Organism-specific databases

    CTDi 823.

    Phylogenomic databases

    eggNOGi NOG327523.
    GeneTreei ENSGT00750000117643.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi Q27970.
    KOi K01367.
    OMAi PQSLGYK.
    OrthoDBi EOG7RV9FM.
    TreeFami TF314748.

    Enzyme and pathway databases

    Reactomei REACT_201925. Degradation of the extracellular matrix.

    Miscellaneous databases

    NextBioi 20805592.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    Pfami PF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness."
      Smith T.P.L., Casas E., Rexroad C.E. III, Kappes S.M., Keele J.W.
      J. Anim. Sci. 78:2589-2594(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Bovine CAPN1 maps to a region containing a QTL for meat tenderness."
      Smith T.P.L., Casas E., Rexroad C.E., Kappes S.M., Keele J.W.
      Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal muscle.
    4. Sun W., Bidwell C.A., Ji S., Hancock D.L.
      Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 530-625.
      Tissue: Skeletal muscle.
    5. "Two-stage autolysis of the catalytic subunit initiates activation of calpain I."
      Zimmerman U.J., Schlaepfer W.W.
      Biochim. Biophys. Acta 1078:192-198(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 16-41.

    Entry informationi

    Entry nameiCAN1_BOVIN
    AccessioniPrimary (citable) accession number: Q27970
    Secondary accession number(s): Q9N0U3, Q9N0V6, Q9N185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3