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Protein

Calpain-1 catalytic subunit

Gene

CAPN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115By similarity1
Active sitei272By similarity1
Active sitei296By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi101 – 1081PROSITE-ProRule annotation8
Calcium bindingi304 – 3352PROSITE-ProRule annotationAdd BLAST32
Calcium bindingi600 – 6113PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi630 – 6414PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.
R-BTA-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiC02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase 1
Short name:
CANP 1
Calpain mu-type
Calpain-1 large subunit
Micromolar-calpain
Short name:
muCANP
Gene namesi
Name:CAPN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002076932 – 716Calpain-1 catalytic subunitAdd BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphothreonineBy similarity1

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei15 – 16Cleavage; for 78 kDa formBy similarity2
Sitei27 – 28Cleavage; for 75 kDa formBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

PaxDbiQ27970.
PeptideAtlasiQ27970.
PRIDEiQ27970.

Expressioni

Gene expression databases

BgeeiENSBTAG00000010230.

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) and a small (regulatory) subunit.

Protein-protein interaction databases

IntActiQ27970. 1 interactor.
STRINGi9913.ENSBTAP00000011678.

Structurei

3D structure databases

ProteinModelPortaliQ27970.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 354Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini543 – 578EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini587 – 620EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini617 – 652EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini682 – 716EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 528Domain IIIAdd BLAST174
Regioni529 – 544LinkerAdd BLAST16
Regioni545 – 715Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27970.
KOiK01367.
OMAiNYQGQMV.
OrthoDBiEOG091G049E.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEFITPVY CTGVSAQVQK QRAKELGLGR HENAIKYLGQ DYEQLRVHCL
60 70 80 90 100
QRGALFRDEA FPPVPQSLGF KELGPNSSKT YGIKWKRPTE LFSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQDGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYNIILK ALERGSLLGC
260 270 280 290 300
SIDISSILDM EAVTFKKLVK GHAYSVTGAK QVNYQGQMVN LIRMRNPWGE
310 320 330 340 350
VEWTGAWSDG SSEWNGVDPY MREQLRVKME DGEFWMSFRD FMREFTRLEI
360 370 380 390 400
CNLTPDALKS QRFRNWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEETDDPDPD DYGGRESGCS FLLALMQKHR RRERRFGRDM ETIGFAVYEV
460 470 480 490 500
PPELMGQPAV HLKRDFFLSN ASRARSEQFI NLREVSTRFR LPPGEYVVVP
510 520 530 540 550
STFEPNKEGD FVLRFFSEKS AGTQELDDQV QANLPDEQVL SEEEIDENFK
560 570 580 590 600
SLFRQLAGED MEISVKELRT ILNRIISKHK DLRTTGFSLE SCRSMVNLMD
610 620 630 640 650
RDGNGKLGLV EFNILWNRIR NYLSIFRKFD LDKSGSMSAY EMRMAIEFAG
660 670 680 690 700
FKLNKKLYEL IITRYSEPDL AVDFDNFVCC LVRLETMFRF FKTLDTDLDG
710
VVTFDLFKWL QLTMFA
Length:716
Mass (Da):82,207
Last modified:December 12, 2006 - v3
Checksum:iA7F55C197BD5DF23
GO

Sequence cautioni

The sequence AAA18454 differs from that shown. Reason: Frameshift at positions 582 and 617.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21Q → T AA sequence (PubMed:2065086).Curated1
Sequence conflicti316G → A in AAF64504 (PubMed:11048924).Curated1
Sequence conflicti381A → V in AAF64504 (PubMed:11048924).Curated1
Sequence conflicti530V → I in AAA18454 (Ref. 4) Curated1
Sequence conflicti624S → A in AAA18454 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248054, AF252504 Genomic DNA. Translation: AAF64504.2.
AF221129 mRNA. Translation: AAF32364.1.
BC123635 mRNA. Translation: AAI23636.1.
U07849 mRNA. Translation: AAA18454.1. Frameshift.
PIRiS16181.
RefSeqiNP_776684.1. NM_174259.2.
UniGeneiBt.252.

Genome annotation databases

EnsembliENSBTAT00000011678; ENSBTAP00000011678; ENSBTAG00000010230.
GeneIDi281661.
KEGGibta:281661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248054, AF252504 Genomic DNA. Translation: AAF64504.2.
AF221129 mRNA. Translation: AAF32364.1.
BC123635 mRNA. Translation: AAI23636.1.
U07849 mRNA. Translation: AAA18454.1. Frameshift.
PIRiS16181.
RefSeqiNP_776684.1. NM_174259.2.
UniGeneiBt.252.

3D structure databases

ProteinModelPortaliQ27970.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ27970. 1 interactor.
STRINGi9913.ENSBTAP00000011678.

Protein family/group databases

MEROPSiC02.001.

Proteomic databases

PaxDbiQ27970.
PeptideAtlasiQ27970.
PRIDEiQ27970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011678; ENSBTAP00000011678; ENSBTAG00000010230.
GeneIDi281661.
KEGGibta:281661.

Organism-specific databases

CTDi823.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ27970.
KOiK01367.
OMAiNYQGQMV.
OrthoDBiEOG091G049E.
TreeFamiTF314748.

Enzyme and pathway databases

ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.
R-BTA-6798695. Neutrophil degranulation.

Gene expression databases

BgeeiENSBTAG00000010230.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN1_BOVIN
AccessioniPrimary (citable) accession number: Q27970
Secondary accession number(s): Q9N0U3, Q9N0V6, Q9N185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 12, 2006
Last modified: November 30, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.