Reviewed, UniProtKB/Swiss-Prot Q27968 (DNJC3_BOVIN)

Names and origin

Protein names

Recommended name:
    DnaJ homolog subfamily C member 3
Alternative name(s):
    Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
    Protein kinase inhibitor of 58 kDa
      Short name=Protein kinase inhibitor p58

Gene names

Name:	DNAJC3
Synonyms:	P58IPK

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. Ref.1
Subunit structure	Interacts with EIF2AK2 and EIF2AK3. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with PRKRIR/P52RIPK By similarity.
Subcellular location	Endoplasmic reticulum By similarity Ref.3.
Domain	The J domain mediates interaction with HSPA8 By similarity.
Sequence similarities	Contains 1 J domain. Contains 9 TPR repeats.

Ontologies

Keywords
Biological process	Unfolded protein response
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal TPR repeat
Molecular function	Chaperone
Gene Ontology (GO)
Biological process	response to unfolded protein Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heat shock protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF2AK2	P19525	3	EBI-640793,EBI-640775	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 504	473	DnaJ homolog subfamily C member 3		PRO_0000071044
Regions
	Repeat	37 – 70	34	TPR 1
	Repeat	72 – 104	33	TPR 2
	Repeat	105 – 138	34	TPR 3
	Repeat	154 – 187	34	TPR 4
	Repeat	189 – 221	33	TPR 5
	Repeat	222 – 255	34	TPR 6
	Repeat	268 – 301	34	TPR 7
	Repeat	306 – 339	34	TPR 8
	Repeat	340 – 373	34	TPR 9
	Domain	394 – 462	69	J
Experimental info
	Sequence conflict	36	1	D → Y in AAI26581. Ref.2
	Sequence conflict	489	1	S → N in AAI26581. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q27968-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2383C36862BD9E4C	FASTA	504	57,704
	10 20 30 40 50 60 MVAPGSVTSR LGSVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA GQLADALSQF 70 80 90 100 110 120 HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIEL KMDFTAARLQ RGHLLLKQGK 130 140 150 160 170 180 LDEAEDDFKK VLKSNPSENE EKEAQSQLVK SDEMQRLRSQ ALDAFESSDF TAAITFLDKI 190 200 210 220 230 240 LEVCVWDAEL RELRAECFIK EGEPRKAISD LKASSKLKND NTEAFYKIST LYYELGDHEL 250 260 270 280 290 300 SLSEVRECLK LDQDHKRCFA HYKQVKKLNK LIESAEELIK EGRYTDAISK YESVMKTEPG 310 320 330 340 350 360 VHEYTIRSKE RICHCFSKDE KPVEAIRVCS EVLQVEPDNV NALKDRAEAY LIEEMYDEAI 370 380 390 400 410 420 QDYETAQEHN ENDQQIREGL EKAQRLLKQS QRRDYYKILG VKRNAKKQEI IKAYRKLALQ 430 440 450 460 470 480 WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR 490 500 SWNSWQGFSP FSSGGPFRFK FHFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 58,000-dalton cellular inhibitor of the interferon-induced double-stranded RNA-activated protein kinase (PKR) is a member of the tetratricopeptide repeat family of proteins." Lee T.G., Tang N., Thompson S., Miller J., Katze M.G. Mol. Cell. Biol. 14:2331-2342(1994) [PubMed: 7511204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Placenta.
[3]	"Cloning, expression, and cellular localization of the oncogenic 58-kDa inhibitor of the RNA-activated human and mouse protein kinase." Korth M.J., Lyons C.N., Wambach M., Katze M.G. Gene 170:181-188(1996) [PubMed: 8666242] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04631 mRNA. Translation: AAA17795.1. BC126580 mRNA. Translation: AAI26581.1.
IPI	IPI00693007.
PIR	A56534.
RefSeq	NP_777181.1.
UniGene	Bt.68903
3D structure databases
SMR	Q27968. Positions 41-376, 341-458, 391-461.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q27968. 1 interaction.
STRING	Q27968.
Genome annotation databases
Ensembl	ENSBTAT00000016001; ENSBTAP00000016001; ENSBTAG00000012065; Bos taurus. [Genome view]
GeneID	286770.
KEGG	bta:286770.
Organism-specific databases
CTD	286770.
Phylogenomic databases
eggNOG	maNOG11861.
HOVERGEN	Q27968.
InParanoid	Q27968.
Family and domain databases
InterPro	IPR001623. DnaJ_N. IPR015609. Hsp40/DnaJ_Rel. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit. G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. SM00028. TPR. 7 hits. [Graphical view]
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. PS50005. TPR. 8 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNJC3_BOVIN

Accession

Primary (citable) accession number: Q27968
Secondary accession number(s): A0JN99

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)