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Protein

DnaJ homolog subfamily C member 3

Gene

DNAJC3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A (PubMed:7511204). May inhibit EIF2AK3/PERK activity (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Repressor

Keywords - Biological processi

Stress response, Translation regulation, Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 3
Alternative name(s):
Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
Protein kinase inhibitor of 58 kDa
Short name:
Protein kinase inhibitor p58
Gene namesi
Name:DNAJC3
Synonyms:P58IPK
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 504473DnaJ homolog subfamily C member 3PRO_0000071044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi248 ↔ 258By similarity
Modified residuei274 – 2741PhosphoserineBy similarity
Disulfide bondi313 ↔ 329By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ27968.
PRIDEiQ27968.

Interactioni

Subunit structurei

Interacts with EIF2AK4/GCN2; this interaction occurs under endoplasmic reticulum (ER) stress, hypothermic and amino acid starving stress conditions and inhibits EIF2AK4/GCN2 kinase activity. Interacts with EIF2AK3. Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with THAP12.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF2AK2P195255EBI-640793,EBI-640775From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi159906. 2 interactions.
IntActiQ27968. 1 interaction.
MINTiMINT-1515180.
STRINGi9913.ENSBTAP00000016001.

Structurei

3D structure databases

ProteinModelPortaliQ27968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7034TPR 1Add
BLAST
Repeati72 – 10433TPR 2Add
BLAST
Repeati105 – 13834TPR 3Add
BLAST
Repeati154 – 18734TPR 4Add
BLAST
Repeati189 – 22133TPR 5Add
BLAST
Repeati222 – 25534TPR 6Add
BLAST
Repeati268 – 30134TPR 7Add
BLAST
Repeati306 – 33934TPR 8Add
BLAST
Repeati340 – 37334TPR 9Add
BLAST
Domaini394 – 46269JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni375 – 39319Flexible linkerBy similarityAdd
BLAST

Domaini

The J domain mediates interaction with HSPA8.By similarity
Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.By similarity

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG0624. Eukaryota.
COG0484. LUCA.
HOGENOMiHOG000193351.
HOVERGENiHBG053820.
InParanoidiQ27968.
KOiK09523.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
1.25.40.10. 3 hits.
InterProiIPR001623. DnaJ_domain.
IPR026901. DNAJC3.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR018704. TPR_21.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR24078:SF318. PTHR24078:SF318. 1 hit.
PfamiPF00226. DnaJ. 1 hit.
PF09976. TPR_21. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF48452. SSF48452. 2 hits.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS50005. TPR. 8 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPGSVTSR LGSVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA
60 70 80 90 100
GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIEL
110 120 130 140 150
KMDFTAARLQ RGHLLLKQGK LDEAEDDFKK VLKSNPSENE EKEAQSQLVK
160 170 180 190 200
SDEMQRLRSQ ALDAFESSDF TAAITFLDKI LEVCVWDAEL RELRAECFIK
210 220 230 240 250
EGEPRKAISD LKASSKLKND NTEAFYKIST LYYELGDHEL SLSEVRECLK
260 270 280 290 300
LDQDHKRCFA HYKQVKKLNK LIESAEELIK EGRYTDAISK YESVMKTEPG
310 320 330 340 350
VHEYTIRSKE RICHCFSKDE KPVEAIRVCS EVLQVEPDNV NALKDRAEAY
360 370 380 390 400
LIEEMYDEAI QDYETAQEHN ENDQQIREGL EKAQRLLKQS QRRDYYKILG
410 420 430 440 450
VKRNAKKQEI IKAYRKLALQ WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS
460 470 480 490 500
DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR SWNSWQGFSP FSSGGPFRFK

FHFN
Length:504
Mass (Da):57,704
Last modified:November 1, 1996 - v1
Checksum:i2383C36862BD9E4C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361D → Y in AAI26581 (Ref. 2) Curated
Sequence conflicti489 – 4891S → N in AAI26581 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04631 mRNA. Translation: AAA17795.1.
BC126580 mRNA. Translation: AAI26581.1.
PIRiA56534.
RefSeqiNP_777181.1. NM_174756.3.
UniGeneiBt.68903.

Genome annotation databases

GeneIDi286770.
KEGGibta:286770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04631 mRNA. Translation: AAA17795.1.
BC126580 mRNA. Translation: AAI26581.1.
PIRiA56534.
RefSeqiNP_777181.1. NM_174756.3.
UniGeneiBt.68903.

3D structure databases

ProteinModelPortaliQ27968.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159906. 2 interactions.
IntActiQ27968. 1 interaction.
MINTiMINT-1515180.
STRINGi9913.ENSBTAP00000016001.

Proteomic databases

PaxDbiQ27968.
PRIDEiQ27968.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi286770.
KEGGibta:286770.

Organism-specific databases

CTDi5611.

Phylogenomic databases

eggNOGiKOG0624. Eukaryota.
COG0484. LUCA.
HOGENOMiHOG000193351.
HOVERGENiHBG053820.
InParanoidiQ27968.
KOiK09523.

Miscellaneous databases

NextBioi20806431.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
1.25.40.10. 3 hits.
InterProiIPR001623. DnaJ_domain.
IPR026901. DNAJC3.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR018704. TPR_21.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR24078:SF318. PTHR24078:SF318. 1 hit.
PfamiPF00226. DnaJ. 1 hit.
PF09976. TPR_21. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF48452. SSF48452. 2 hits.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS50005. TPR. 8 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The 58,000-dalton cellular inhibitor of the interferon-induced double-stranded RNA-activated protein kinase (PKR) is a member of the tetratricopeptide repeat family of proteins."
    Lee T.G., Tang N., Thompson S., Miller J., Katze M.G.
    Mol. Cell. Biol. 14:2331-2342(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Placenta.
  3. "Cloning, expression, and cellular localization of the oncogenic 58-kDa inhibitor of the RNA-activated human and mouse protein kinase."
    Korth M.J., Lyons C.N., Wambach M., Katze M.G.
    Gene 170:181-188(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDNJC3_BOVIN
AccessioniPrimary (citable) accession number: Q27968
Secondary accession number(s): A0JN99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.