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Protein

Voltage-gated potassium channel subunit beta-2

Gene

KCNAB2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (By similarity). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (By similarity). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (By similarity). Promotes KCNA4 channel closure (By similarity). Modulates the functional properties of KCNA5 (By similarity). Enhances KCNB2 channel activity (By similarity). Binds NADPH and has NADPH-dependent aldoketoreductase activity (By similarity). Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo-trans-2-nonenal (in vitro) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631NADPBy similarity
Binding sitei85 – 851NADPBy similarity
Active sitei90 – 901Proton donor/acceptorBy similarity
Binding sitei90 – 901NADPBy similarity
Binding sitei214 – 2141NADPBy similarity
Binding sitei254 – 2541NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 572NADPBy similarity
Nucleotide bindingi188 – 1892NADPBy similarity
Nucleotide bindingi243 – 2486NADPBy similarity
Nucleotide bindingi323 – 3297NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

NADP, Potassium

Enzyme and pathway databases

ReactomeiR-BTA-1296072. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated potassium channel subunit beta-2 (EC:1.1.1.-By similarity)
Alternative name(s):
K(+) channel subunit beta-2
Kv-beta-2
Gene namesi
Name:KCNAB2
Synonyms:KVB2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane 1 Publication; Peripheral membrane protein Curated; Cytoplasmic side Curated
  • Cell membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
  • Cell projectionaxon 1 Publication
  • Cell junctionsynapsesynaptosome By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits. Associates with microtubules when unphosphorylated.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Voltage-gated potassium channel subunit beta-2PRO_0000148745Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91PhosphoserineBy similarity
Modified residuei20 – 201PhosphoserineBy similarity
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei112 – 1121PhosphoserineBy similarity
Modified residuei124 – 1241N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated by PRKCZ; may be regulated by incorporation in a complex composed of PRKCZ and SQSTM1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ27955.
PRIDEiQ27955.

Expressioni

Tissue specificityi

Detected in the juxtaparanodal region of nodes of Ranvier in myelinated nerve fibers in the spinal cord (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ27955. baseline.

Interactioni

Subunit structurei

Homotetramer (By similarity). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (By similarity). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (By similarity). Interacts with KCNA1 (PubMed:11086297). Interacts with KCNA2 (PubMed:11086297). Interacts with KCNA4 and KCND3 (By similarity). Interacts with KCNA5 (By similarity). Interacts with KCNB2 (By similarity). Interacts (in unphosphorylated form) with MAPRE1 (By similarity). Forms a ternary complex with SQSTM1 and PRKCZ (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042823.

Structurei

3D structure databases

ProteinModelPortaliQ27955.
SMRiQ27955. Positions 37-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

In contrast to KCNAB1, the shorter N-terminal domain of KCNAB2 cannot mediate closure of delayed rectifier potassium channels by physically obstructing the pore.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
GeneTreeiENSGT00550000074567.
HOGENOMiHOG000250283.
HOVERGENiHBG052216.
InParanoidiQ27955.
KOiK04883.
OMAiCINNLEF.
OrthoDBiEOG71G9TR.
TreeFamiTF324563.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005401. K_chnl_volt-dep_bsu_KCNAB2.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01579. KCNAB2CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPESTTGSP ARLSLRQTGS PGMIYSTRYG SPKRQLQFYR NLGKSGLRVS
60 70 80 90 100
CLGLGTWVTF GGQITDEMAE QLMTLAYDNG INLFDTAEVY AAGKAEVVLG
110 120 130 140 150
NIIKKKGWRR SSLVITTKIF WGGKAETERG LSRKHIIEGL KASLERLQLE
160 170 180 190 200
YVDVVFANRP DPNTPMEETV RAMTHVINQG MAMYWGTSRW SSMEIMEAYS
210 220 230 240 250
VARQFNLIPP ICEQAEYHMF QREKVEVQLP ELFHKIGVGA MTWSPLACGI
260 270 280 290 300
VSGKYDSGIP PYSRASLKGY QWLKDKILSE EGRRQQAKLK ELQAIAERLG
310 320 330 340 350
CTLPQLAIAW CLRNEGVSSV LLGASSADQL MENIGAIQVL PKLSSSIIHE
360
IDSILGNKPY SKKDYRS
Length:367
Mass (Da):40,985
Last modified:November 1, 1996 - v1
Checksum:i2C8B5EDED031AED8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70661 mRNA. Translation: CAA49999.1.
AY950785 mRNA. Translation: AAX51985.1.
PIRiA53131.
RefSeqiNP_001014405.1. NM_001014383.1.
XP_005217169.1. XM_005217112.3.
UniGeneiBt.37440.

Genome annotation databases

EnsembliENSBTAT00000045435; ENSBTAP00000042823; ENSBTAG00000008125.
GeneIDi541597.
KEGGibta:541597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70661 mRNA. Translation: CAA49999.1.
AY950785 mRNA. Translation: AAX51985.1.
PIRiA53131.
RefSeqiNP_001014405.1. NM_001014383.1.
XP_005217169.1. XM_005217112.3.
UniGeneiBt.37440.

3D structure databases

ProteinModelPortaliQ27955.
SMRiQ27955. Positions 37-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042823.

Proteomic databases

PaxDbiQ27955.
PRIDEiQ27955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000045435; ENSBTAP00000042823; ENSBTAG00000008125.
GeneIDi541597.
KEGGibta:541597.

Organism-specific databases

CTDi8514.

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
GeneTreeiENSGT00550000074567.
HOGENOMiHOG000250283.
HOVERGENiHBG052216.
InParanoidiQ27955.
KOiK04883.
OMAiCINNLEF.
OrthoDBiEOG71G9TR.
TreeFamiTF324563.

Enzyme and pathway databases

ReactomeiR-BTA-1296072. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi20879363.

Gene expression databases

ExpressionAtlasiQ27955. baseline.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005401. K_chnl_volt-dep_bsu_KCNAB2.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01579. KCNAB2CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of a beta subunit of alpha-dendrotoxin-sensitive K+ channels from bovine brain."
    Scott V.E.S., Rettig J., Parcej D.N., Keen J.N., Findlay J.B.C., Pongs O., Dolly J.O.
    Proc. Natl. Acad. Sci. U.S.A. 91:1637-1641(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-18; 78-107; 198-215; 217-233 AND 266-294.
    Tissue: Brain.
  2. "Ion channels in lens epithelia."
    Rae J.L.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens epithelium.
  3. "Subunit composition and novel localization of K+ channels in spinal cord."
    Rasband M.N., Trimmer J.S.
    J. Comp. Neurol. 429:166-176(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNA1 AND KCNA2, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCAB2_BOVIN
AccessioniPrimary (citable) accession number: Q27955
Secondary accession number(s): Q58HC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.