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Protein

Coatomer subunit alpha

Gene

COPA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
HEP-COP
Short name:
HEPCOP
Cleaved into the following 2 chains:
Alternative name(s):
Xenopsin-related peptide
Gene namesi
Name:COPA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Xenin :

GO - Cellular componenti

  • COPI vesicle coat Source: UniProtKB
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12241224Coatomer subunit alphaPRO_0000223305Add
BLAST
Peptidei1 – 3535ProxeninPRO_0000041398Add
BLAST
Peptidei1 – 2525XeninPRO_0000041399Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731PhosphoserineBy similarity
Modified residuei185 – 1851PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ27954.
PRIDEiQ27954.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1. Interacts with JAGN1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
COPB2P356052EBI-620400,EBI-620411
COPEQ281045EBI-620400,EBI-620457

Protein-protein interaction databases

IntActiQ27954. 2 interactions.
STRINGi9913.ENSBTAP00000005672.

Structurei

Secondary structure

1
1224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi916 – 9238Combined sources
Helixi927 – 9326Combined sources
Helixi936 – 94611Combined sources
Helixi953 – 9553Combined sources
Helixi956 – 9638Combined sources
Beta strandi968 – 9703Combined sources
Beta strandi979 – 9813Combined sources
Turni985 – 9895Combined sources
Beta strandi992 – 9943Combined sources
Helixi1003 – 101816Combined sources
Helixi1022 – 103514Combined sources
Helixi1036 – 10383Combined sources
Helixi1044 – 107027Combined sources
Helixi1078 – 109114Combined sources
Helixi1098 – 111417Combined sources
Helixi1118 – 113013Combined sources
Helixi1135 – 115016Combined sources
Beta strandi1154 – 11563Combined sources
Turni1170 – 11723Combined sources
Turni1186 – 11883Combined sources
Helixi1194 – 11963Combined sources
Turni1202 – 12043Combined sources
Beta strandi1205 – 12106Combined sources
Helixi1220 – 12223Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MKRX-ray2.60B905-1224[»]
ProteinModelPortaliQ27954.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ27954.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati7 – 3731WD 1Add
BLAST
Repeati49 – 7931WD 2Add
BLAST
Repeati91 – 12131WD 3Add
BLAST
Repeati133 – 16331WD 4Add
BLAST
Repeati203 – 23331WD 5Add
BLAST
Repeati247 – 27731WD 6Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0292. Eukaryota.
ENOG410XPZS. LUCA.
HOGENOMiHOG000195913.
HOVERGENiHBG005379.
InParanoidiQ27954.
KOiK05236.
OrthoDBiEOG78WKQX.
TreeFamiTF105693.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH
60 70 80 90 100
DGPVRGIDFH KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF
110 120 130 140 150
FHHEYPWILS ASDDQTIRVW NWQSRTCVCV LTGHNHYVMC AQFHPSEDLV
160 170 180 190 200
VSASLDQTVR VWDISGLRKK NLSPGAVESD VRGITGVDLF GTTDAVVKHV
210 220 230 240 250
LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW EVDTCRGHYN
260 270 280 290 300
NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH
310 320 330 340 350
PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK
360 370 380 390 400
DVAVMQLRSG SKFPVFSMSY NPAENAVLLC TRASNLENST YDLYTIPKDA
410 420 430 440 450
DTQNPDAPEG KRSSGLTAVW VARNRFAVLD RMHSLLIKNL KNEITKKVQV
460 470 480 490 500
PNCDEIFYAG TGNLLLRDAE SITLFDVQQK RTLASVKISK VKYVIWSADM
510 520 530 540 550
SHVALLAKHA IVICNRKLEA LCNIHENIRV KSGAWDESGV FIYTTSNHIK
560 570 580 590 600
YAVTTGDYGI IRTLDLPIYV TRVKGNNVYC LDRECRPRVL TIDPTEFKFK
610 620 630 640 650
LALINRKYDE VLHMVRNAKL VGQSIIAYLQ KKGYPEVALH FVKDEKTRFS
660 670 680 690 700
LALECGNIEI ALEAAKALDD KNCWEKLGEV ALLQGNHQIV EMCYQRTKNF
710 720 730 740 750
DKLSFLYLIT GNLEKLRKMM KIAEIRKDMS GHYQNALYLG DVSERVRILK
760 770 780 790 800
NCGQKSLAYL TAATHGLDEE AESLKETFDP EKETIPDIDP NAKLLQPPAP
810 820 830 840 850
IMPLDTNWPL LTVSKGFFEG SIASKGKGGA LAADIDIDTV GTEGWGEDAE
860 870 880 890 900
LQLDEDGFVE ATEGLGDDAL GKGQEEGGGW DVEEDLELPP ELDIPPGAAG
910 920 930 940 950
GAEDGFFVPP TKGTSPTQIW CNNSQLPVDH ILAGSFETAM RLLHDQVGVT
960 970 980 990 1000
QFGPYKQLFL QTYARGRTTY QALPCLPSMY GYPNRNWKDA GLKNGVPAVG
1010 1020 1030 1040 1050
LKLNDLIQRL QLCYQLTTVG KFEEAVEKFR SILLSVPLLV VDNKQEIAEA
1060 1070 1080 1090 1100
QQLITICREY IVGLSMETER KKLPKETLEQ QKRICEMAAY FTHSNLQPVH
1110 1120 1130 1140 1150
MILVLRTALN LFFKLKNFRT AAAFARRLLE LGPKPEVAQQ TRKILSACEK
1160 1170 1180 1190 1200
NPTDAYQLNY DMHNPFDICA ASYRPIYRGK PVEKCPLSGA CYSPEFKGQI
1210 1220
CKVTTVTEIG KDVIGLRISP LQFR
Length:1,224
Mass (Da):138,359
Last modified:November 1, 1997 - v1
Checksum:iC9350BF2AC00683D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti402 – 4021T → S in AAI53252 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96768 mRNA. Translation: CAA65543.1.
BC153251 mRNA. Translation: AAI53252.1.
RefSeqiNP_001099115.1. NM_001105645.1.
UniGeneiBt.76408.

Genome annotation databases

GeneIDi100126041.
KEGGibta:100126041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96768 mRNA. Translation: CAA65543.1.
BC153251 mRNA. Translation: AAI53252.1.
RefSeqiNP_001099115.1. NM_001105645.1.
UniGeneiBt.76408.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MKRX-ray2.60B905-1224[»]
ProteinModelPortaliQ27954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ27954. 2 interactions.
STRINGi9913.ENSBTAP00000005672.

Proteomic databases

PaxDbiQ27954.
PRIDEiQ27954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100126041.
KEGGibta:100126041.

Organism-specific databases

CTDi1314.

Phylogenomic databases

eggNOGiKOG0292. Eukaryota.
ENOG410XPZS. LUCA.
HOGENOMiHOG000195913.
HOVERGENiHBG005379.
InParanoidiQ27954.
KOiK05236.
OrthoDBiEOG78WKQX.
TreeFamiTF105693.

Miscellaneous databases

EvolutionaryTraceiQ27954.
NextBioi20788926.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Architecture of coatomer: molecular characterization of delta-COP and protein interactions within the complex."
    Faulstich D., Auerbach S., Orci L., Ravazzola M., Wegehingel S., Lottspeich F., Stenbeck G., Harter C., Wieland F.T., Tschochner H.
    J. Cell Biol. 135:53-61(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-25; 161-168; 296-306; 314-331; 383-398; 413-431; 518-529; 676-691; 728-742; 776-793; 968-980; 1010-1020; 1087-1100 AND 1202-1208.
    Tissue: Liver.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.
  3. Cited for: INTERACTION WITH PEX11A.

Entry informationi

Entry nameiCOPA_BOVIN
AccessioniPrimary (citable) accession number: Q27954
Secondary accession number(s): A7Z053
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.