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Q27928

- GPDA_DROPS

UniProt

Q27928 - GPDA_DROPS

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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene
Gpdh
Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981NAD By similarity
Binding sitei121 – 1211NAD; via amide nitrogen By similarity
Binding sitei121 – 1211Substrate By similarity
Binding sitei155 – 1551NAD; via amide nitrogen By similarity
Active sitei206 – 2061Proton acceptor By similarity
Binding sitei270 – 2701NAD By similarity
Binding sitei299 – 2991NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166NAD By similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate catabolic process Source: InterPro
  3. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00086.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
Short name:
GPD-C
Short name:
GPDH-C
Gene namesi
Name:Gpdh
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0243577. Dpse\Gpdh.

Subcellular locationi

GO - Cellular componenti

  1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138076Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ27928.
SMRiQ27928. Positions 1-350.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni270 – 2712Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0240.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q27928-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MADKVNVCIV GSGNWGSAIA KIVGANASAL PEFEERVTMF VYEEMIDGKK    50
LTEIINETHE NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP 100
NFCKQLLGKI KPNAIAISLI KGFDKAEGGG IDLISHIITR HLKIPCAVLM 150
GANLANEVAE GNFCETTIGC TDKKYGKVLR DLFQANHFRV VVVDDADAVE 200
VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF VDVFYPGSKL 250
STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 300
PPTAEEVNYM LKNKKLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM 350
DTSIMPSPKL 360
Length:360
Mass (Da):39,448
Last modified:April 29, 2008 - v2
Checksum:i1E5A5AB76C741998
GO
Isoform B (identifier: Q27928-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.

Show »
Length:350
Mass (Da):38,378
Checksum:i9D278256379A1060
GO
Isoform C (identifier: Q27928-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-359: DTSIMPSPK → QT

Show »
Length:353
Mass (Da):38,720
Checksum:iAACAEC2D27825637
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei351 – 36010Missing in isoform B. VSP_033248
Alternative sequencei351 – 3599DTSIMPSPK → QT in isoform C. VSP_033249

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781P → L in AAC37298. 1 Publication
Sequence conflicti347 – 3471P → L in AAC37298. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59682 Genomic DNA. Translation: AAB02946.1.
U59682 Genomic DNA. Translation: AAB02947.1.
U59682 Genomic DNA. Translation: AAB02948.1.
CH379058 Genomic DNA. No translation available.
AY754559 Genomic DNA. Translation: AAX13134.1.
L41249 Genomic DNA. Translation: AAC37298.1.
U47885 Genomic DNA. Translation: AAB50306.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59682 Genomic DNA. Translation: AAB02946.1 .
U59682 Genomic DNA. Translation: AAB02947.1 .
U59682 Genomic DNA. Translation: AAB02948.1 .
CH379058 Genomic DNA. No translation available.
AY754559 Genomic DNA. Translation: AAX13134.1 .
L41249 Genomic DNA. Translation: AAC37298.1 .
U47885 Genomic DNA. Translation: AAB50306.1 .

3D structure databases

ProteinModelPortali Q27928.
SMRi Q27928. Positions 1-350.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

FlyBasei FBgn0243577. Dpse\Gpdh.

Phylogenomic databases

eggNOGi COG0240.

Enzyme and pathway databases

UniPathwayi UPA00086 .

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
PROSITEi PS00957. NAD_G3PDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and evolution of the Drosophila pseudoobscura glycerol-3-phosphate dehydrogenase locus."
    Wells R.S.
    J. Mol. Evol. 41:886-893(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A; B AND C).
    Strain: JR45.
  2. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.
  3. "Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
    Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
    Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-137.
  4. "Erratic evolution of glycerol-3-phosphate dehydrogenase in Drosophila, Chymomyza, and Ceratitis."
    Kwiatowski J., Krawczyk M., Jaworski M., Skarecky D., Ayala F.J.
    J. Mol. Evol. 44:9-22(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-360.
  5. "Evolution of the Drosophila obscura species group inferred from the Gpdh and Sod genes."
    Barrio E., Ayala F.J.
    Mol. Phylogenet. Evol. 7:79-93(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 88-340.
    Strain: NDSSC 14011-0121.43.

Entry informationi

Entry nameiGPDA_DROPS
AccessioniPrimary (citable) accession number: Q27928
Secondary accession number(s): O02631, Q56RE8, Q95089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 29, 2008
Last modified: October 16, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi