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Q27928 (GPDA_DROPS) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Short name=GPD-C
Short name=GPDH-C
EC=1.1.1.8
Gene names
Name:Gpdh
OrganismDrosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]
Taxonomic identifier46245 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Pathway

Phospholipid metabolism; alpha-glycerophosphate cycle.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q27928-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q27928-2)

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.
Isoform C (identifier: Q27928-3)

The sequence of this isoform differs from the canonical sequence as follows:
     351-359: DTSIMPSPK → QT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
PRO_0000138076

Regions

Nucleotide binding11 – 166NAD By similarity
Region270 – 2712Substrate binding By similarity

Sites

Active site2061Proton acceptor By similarity
Binding site981NAD By similarity
Binding site1211NAD; via amide nitrogen By similarity
Binding site1211Substrate By similarity
Binding site1551NAD; via amide nitrogen By similarity
Binding site2701NAD By similarity
Binding site2991NAD By similarity

Natural variations

Alternative sequence351 – 36010Missing in isoform B.
VSP_033248
Alternative sequence351 – 3599DTSIMPSPK → QT in isoform C.
VSP_033249

Experimental info

Sequence conflict781P → L in AAC37298. Ref.4
Sequence conflict3471P → L in AAC37298. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 1E5A5AB76C741998

FASTA36039,448
        10         20         30         40         50         60 
MADKVNVCIV GSGNWGSAIA KIVGANASAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 

        70         80         90        100        110        120 
NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 

       130        140        150        160        170        180 
KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 

       190        200        210        220        230        240 
DLFQANHFRV VVVDDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 

       250        260        270        280        290        300 
VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 

       310        320        330        340        350        360 
PPTAEEVNYM LKNKKLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM DTSIMPSPKL 

« Hide

Isoform B [UniParc].

Checksum: 9D278256379A1060
Show »

FASTA35038,378
Isoform C [UniParc].

Checksum: AACAEC2D27825637
Show »

FASTA35338,720

References

« Hide 'large scale' references
[1]"Sequence and evolution of the Drosophila pseudoobscura glycerol-3-phosphate dehydrogenase locus."
Wells R.S.
J. Mol. Evol. 41:886-893(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A; B AND C).
Strain: JR45.
[2]"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. expand/collapse author list , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MV2-25 / Tucson 14011-0121.94.
[3]"Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-137.
[4]"Erratic evolution of glycerol-3-phosphate dehydrogenase in Drosophila, Chymomyza, and Ceratitis."
Kwiatowski J., Krawczyk M., Jaworski M., Skarecky D., Ayala F.J.
J. Mol. Evol. 44:9-22(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-360.
[5]"Evolution of the Drosophila obscura species group inferred from the Gpdh and Sod genes."
Barrio E., Ayala F.J.
Mol. Phylogenet. Evol. 7:79-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 88-340.
Strain: NDSSC 14011-0121.43.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59682 Genomic DNA. Translation: AAB02946.1.
U59682 Genomic DNA. Translation: AAB02947.1.
U59682 Genomic DNA. Translation: AAB02948.1.
CH379058 Genomic DNA. No translation available.
AY754559 Genomic DNA. Translation: AAX13134.1.
L41249 Genomic DNA. Translation: AAC37298.1.
U47885 Genomic DNA. Translation: AAB50306.1.

3D structure databases

ProteinModelPortalQ27928.
SMRQ27928. Positions 1-350.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

FlyBaseFBgn0243577. Dpse\Gpdh.

Phylogenomic databases

eggNOGCOG0240.

Enzyme and pathway databases

UniPathwayUPA00086.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPDA_DROPS
AccessionPrimary (citable) accession number: Q27928
Secondary accession number(s): O02631, Q56RE8, Q95089
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 29, 2008
Last modified: October 16, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase