Reviewed, UniProtKB/Swiss-Prot Q27928 (GPDA_DROPS)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
      Short name=GPDH-C
      Short name=GPD-C
    EC=1.1.1.8

Gene names

Name:

Gpdh

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Pathway	Phospholipid metabolism; alpha-glycerophosphate cycle.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q27928-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q27928-2)

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.

Isoform C (identifier: Q27928-3)

The sequence of this isoform differs from the canonical sequence as follows:
     351-359: DTSIMPSPK → QT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic		PRO_0000138076
Regions
	Nucleotide binding	11 – 16	6	NAD By similarity
	Region	270 – 271	2	Substrate binding By similarity
Sites
	Active site	206	1	Proton acceptor By similarity
	Binding site	98	1	NAD By similarity
	Binding site	121	1	NAD; via amide nitrogen By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	155	1	NAD; via amide nitrogen By similarity
	Binding site	270	1	NAD By similarity
	Binding site	299	1	NAD By similarity
Natural variations
	Alternative sequence	351 – 360	10	Missing in isoform B.		VSP_033248
	Alternative sequence	351 – 359	9	DTSIMPSPK → QT in isoform C.		VSP_033249
Experimental info
	Sequence conflict	78	1	P → L in AAC37298. Ref.4
	Sequence conflict	347	1	P → L in AAC37298. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 1E5A5AB76C741998	FASTA	360	39,448
	10 20 30 40 50 60 MADKVNVCIV GSGNWGSAIA KIVGANASAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 70 80 90 100 110 120 NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 130 140 150 160 170 180 KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 190 200 210 220 230 240 DLFQANHFRV VVVDDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 250 260 270 280 290 300 VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 310 320 330 340 350 360 PPTAEEVNYM LKNKKLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM DTSIMPSPKL
	Isoform B [UniParc]. Checksum: 9D278256379A1060		350	38,378
	10 20 30 40 50 60 MADKVNVCIV GSGNWGSAIA KIVGANASAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 70 80 90 100 110 120 NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 130 140 150 160 170 180 KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 190 200 210 220 230 240 DLFQANHFRV VVVDDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 250 260 270 280 290 300 VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 310 320 330 340 350 PPTAEEVNYM LKNKKLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM
	Isoform C [UniParc]. Checksum: AACAEC2D27825637		353	38,720
	10 20 30 40 50 60 MADKVNVCIV GSGNWGSAIA KIVGANASAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 70 80 90 100 110 120 NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 130 140 150 160 170 180 KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 190 200 210 220 230 240 DLFQANHFRV VVVDDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 250 260 270 280 290 300 VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 310 320 330 340 350 PPTAEEVNYM LKNKKLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM QTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and evolution of the Drosophila pseudoobscura glycerol-3-phosphate dehydrogenase locus." Wells R.S. J. Mol. Evol. 41:886-893(1995) [PubMed: 8587133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A; B AND C). Strain: JR45.
[2]	"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.
[3]	"Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda." Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B. Genetics 169:1495-1507(2005) [PubMed: 15545653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-137.
[4]	"Erratic evolution of glycerol-3-phosphate dehydrogenase in Drosophila, Chymomyza, and Ceratitis." Kwiatowski J., Krawczyk M., Jaworski M., Skarecky D., Ayala F.J. J. Mol. Evol. 44:9-22(1997) [PubMed: 9010132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-360.
[5]	"Evolution of the Drosophila obscura species group inferred from the Gpdh and Sod genes." Barrio E., Ayala F.J. Mol. Phylogenet. Evol. 7:79-93(1997) [PubMed: 9007023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 88-340. Strain: NDSSC 14011-0121.43.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U59682 Genomic DNA. Translation: AAB02946.1. U59682 Genomic DNA. Translation: AAB02947.1. U59682 Genomic DNA. Translation: AAB02948.1. CH379058 Genomic DNA. No translation available. AY754559 Genomic DNA. Translation: AAX13134.1. L41249 Genomic DNA. Translation: AAC37298.1. U47885 Genomic DNA. Translation: AAB50306.1.
3D structure databases
ModBase	Search...
Organism-specific databases
FlyBase	FBgn0243577. Dpse\Gpdh.
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_DROPS

Accession

Primary (citable) accession number: Q27928
Secondary accession number(s): O02631, Q56RE8, Q95089

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	April 29, 2008
Last modified:	November 25, 2008
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project