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Q27928

- GPDA_DROPS

UniProt

Q27928 - GPDA_DROPS

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpdh

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei98 – 981NADBy similarity
    Binding sitei121 – 1211NAD; via amide nitrogenBy similarity
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei155 – 1551NAD; via amide nitrogenBy similarity
    Active sitei206 – 2061Proton acceptorBy similarity
    Binding sitei270 – 2701NADBy similarity
    Binding sitei299 – 2991NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 166NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycerol-3-phosphate catabolic process Source: InterPro
    3. glycerophospholipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00086.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
    Short name:
    GPD-C
    Short name:
    GPDH-C
    Gene namesi
    Name:Gpdh
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0243577. Dpse\Gpdh.

    Subcellular locationi

    GO - Cellular componenti

    1. glycerol-3-phosphate dehydrogenase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138076Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ27928.
    SMRiQ27928. Positions 1-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni270 – 2712Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0240.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q27928-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADKVNVCIV GSGNWGSAIA KIVGANASAL PEFEERVTMF VYEEMIDGKK    50
    LTEIINETHE NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP 100
    NFCKQLLGKI KPNAIAISLI KGFDKAEGGG IDLISHIITR HLKIPCAVLM 150
    GANLANEVAE GNFCETTIGC TDKKYGKVLR DLFQANHFRV VVVDDADAVE 200
    VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF VDVFYPGSKL 250
    STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 300
    PPTAEEVNYM LKNKKLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM 350
    DTSIMPSPKL 360
    Length:360
    Mass (Da):39,448
    Last modified:April 29, 2008 - v2
    Checksum:i1E5A5AB76C741998
    GO
    Isoform B (identifier: Q27928-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         351-360: Missing.

    Show »
    Length:350
    Mass (Da):38,378
    Checksum:i9D278256379A1060
    GO
    Isoform C (identifier: Q27928-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         351-359: DTSIMPSPK → QT

    Show »
    Length:353
    Mass (Da):38,720
    Checksum:iAACAEC2D27825637
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti78 – 781P → L in AAC37298. (PubMed:9010132)Curated
    Sequence conflicti347 – 3471P → L in AAC37298. (PubMed:9010132)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei351 – 36010Missing in isoform B. CuratedVSP_033248
    Alternative sequencei351 – 3599DTSIMPSPK → QT in isoform C. CuratedVSP_033249

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59682 Genomic DNA. Translation: AAB02946.1.
    U59682 Genomic DNA. Translation: AAB02947.1.
    U59682 Genomic DNA. Translation: AAB02948.1.
    CH379058 Genomic DNA. No translation available.
    AY754559 Genomic DNA. Translation: AAX13134.1.
    L41249 Genomic DNA. Translation: AAC37298.1.
    U47885 Genomic DNA. Translation: AAB50306.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59682 Genomic DNA. Translation: AAB02946.1 .
    U59682 Genomic DNA. Translation: AAB02947.1 .
    U59682 Genomic DNA. Translation: AAB02948.1 .
    CH379058 Genomic DNA. No translation available.
    AY754559 Genomic DNA. Translation: AAX13134.1 .
    L41249 Genomic DNA. Translation: AAC37298.1 .
    U47885 Genomic DNA. Translation: AAB50306.1 .

    3D structure databases

    ProteinModelPortali Q27928.
    SMRi Q27928. Positions 1-350.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    FlyBasei FBgn0243577. Dpse\Gpdh.

    Phylogenomic databases

    eggNOGi COG0240.

    Enzyme and pathway databases

    UniPathwayi UPA00086 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and evolution of the Drosophila pseudoobscura glycerol-3-phosphate dehydrogenase locus."
      Wells R.S.
      J. Mol. Evol. 41:886-893(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A; B AND C).
      Strain: JR45.
    2. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.
    3. "Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
      Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
      Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-137.
    4. "Erratic evolution of glycerol-3-phosphate dehydrogenase in Drosophila, Chymomyza, and Ceratitis."
      Kwiatowski J., Krawczyk M., Jaworski M., Skarecky D., Ayala F.J.
      J. Mol. Evol. 44:9-22(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-360.
    5. "Evolution of the Drosophila obscura species group inferred from the Gpdh and Sod genes."
      Barrio E., Ayala F.J.
      Mol. Phylogenet. Evol. 7:79-93(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 88-340.
      Strain: NDSSC 14011-0121.43.

    Entry informationi

    Entry nameiGPDA_DROPS
    AccessioniPrimary (citable) accession number: Q27928
    Secondary accession number(s): O02631, Q56RE8, Q95089
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3