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Q27902 (CP6B4_PAPGL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 6B4

EC=1.14.14.1
Alternative name(s):
CYP6B4v1/CYP6B4v2
CYPVIB4
Gene names
Name:CYP6B4
OrganismPapilio glaucus (Eastern tiger swallowtail butterfly)
Taxonomic identifier45779 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins. This isozyme metabolizes isopimpinellin, imperatorin, and bergapten at high rates, xanthotoxin and psoralen at intermediate rates and angelicin, sphondin, and trioxsalen only at very low rates.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By furnocoumarin.

Polymorphism

The sequence shown is that of 6B4-1, 6B4-2 seems to differ in 7 positions and is probably an allele.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Cytochrome P450 6B4
PRO_0000051896

Sites

Metal binding4431Iron (heme axial ligand) By similarity

Natural variations

Natural variant171F → L in 6B4-2.
Natural variant1601F → I in 6B4-2.
Natural variant2291I → L in 6B4-2.
Natural variant2861L → F in 6B4-2.
Natural variant4771N → S in 6B4-2.
Natural variant4811K → T in 6B4-2.
Natural variant4961I → L in 6B4-2.

Sequences

Sequence LengthMass (Da)Tools
Q27902 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F6042D7E196029E2

FASTA50058,046
        10         20         30         40         50         60 
MLTIFIVTAT LFAILYFYFT RNFNYWKDRN VVGPEPTVFF GNIMESVIRR KHLIMIYKDI 

        70         80         90        100        110        120 
YEAFPKEKVV GIYRMTTPCL LLRDLDVIKH VMIKDFDLFN DRGVEFSEEG LGLNIFHADG 

       130        140        150        160        170        180 
DRWRVLRQCF TPLFTSGKLK NMLNLMSDRG DKFIKMVEKF CDKEPEQQII PLVRKFTMAS 

       190        200        210        220        230        240 
ITTCAFGMEL DEEMIETLDK LDSLIFTTSY GNEIDMMYPG ILKKLNSSIF SKMIAPFFDN 

       250        260        270        280        290        300 
LTKTIIEQRG GKPTNRKDLM DLILELRQKK AIEPMKKTHD EQVTTLELTD SVIAAQTFIF 

       310        320        330        340        350        360 
YAAGYETSAS TMSFLLFELA ENPDIQEKVI AEVDETLKRH NGEITYDTLS EMTYLTQVFH 

       370        380        390        400        410        420 
ETLRKYPVAD ILLRNAKADY AVPGTNVTLK KGQTVVVSGF GIHYDPKYYP DPEKFDPERF 

       430        440        450        460        470        480 
SPENVRNRHP CAYIPFGAGQ RKCLGMRFGQ WQVQVCIIKL LSKFRFEPST KTMSEFNYDP 

       490        500 
KRLLVYPKSG IFLNIIPRNY 

« Hide

References

[1]"Isolation and characterization of CYP6B4, a furanocoumarin-inducible cytochrome P450 from a polyphagous caterpillar (Lepidoptera: Papilionidae)."
Hung C.F., Berenbaum M.R., Schuler M.A.
Insect Biochem. Mol. Biol. 27:377-385(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47059 mRNA. Translation: AAB05892.1.
U65489 Genomic DNA. Translation: AAB06742.1.

3D structure databases

ProteinModelPortalQ27902.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP6B4_PAPGL
AccessionPrimary (citable) accession number: Q27902
Secondary accession number(s): Q95035
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families