Q27902 (CP6B4_PAPGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 6B4
EC=1.14.14.1

Alternative name(s):
CYP6B4v1/CYP6B4v2
CYPVIB4

Gene names

Name:

CYP6B4

Organism

Papilio glaucus (Eastern tiger swallowtail butterfly)

Taxonomic identifier

45779 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Papilionoidea › Papilionidae › Papilioninae › Papilionini › Papilio

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins. This isozyme metabolizes isopimpinellin, imperatorin, and bergapten at high rates, xanthotoxin and psoralen at intermediate rates and angelicin, sphondin, and trioxsalen only at very low rates.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By furnocoumarin.
Polymorphism	The sequence shown is that of 6B4-1, 6B4-2 seems to differ in 7 positions and is probably an allele.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Cytochrome P450 6B4		PRO_0000051896
Sites
	Metal binding	443	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	17	1	F → L in 6B4-2.
	Natural variant	160	1	F → I in 6B4-2.
	Natural variant	229	1	I → L in 6B4-2.
	Natural variant	286	1	L → F in 6B4-2.
	Natural variant	477	1	N → S in 6B4-2.
	Natural variant	481	1	K → T in 6B4-2.
	Natural variant	496	1	I → L in 6B4-2.

Sequences

	Sequence		Length	Mass (Da)
	Q27902 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F6042D7E196029E2	FASTA	500	58,046
	10 20 30 40 50 60 MLTIFIVTAT LFAILYFYFT RNFNYWKDRN VVGPEPTVFF GNIMESVIRR KHLIMIYKDI 70 80 90 100 110 120 YEAFPKEKVV GIYRMTTPCL LLRDLDVIKH VMIKDFDLFN DRGVEFSEEG LGLNIFHADG 130 140 150 160 170 180 DRWRVLRQCF TPLFTSGKLK NMLNLMSDRG DKFIKMVEKF CDKEPEQQII PLVRKFTMAS 190 200 210 220 230 240 ITTCAFGMEL DEEMIETLDK LDSLIFTTSY GNEIDMMYPG ILKKLNSSIF SKMIAPFFDN 250 260 270 280 290 300 LTKTIIEQRG GKPTNRKDLM DLILELRQKK AIEPMKKTHD EQVTTLELTD SVIAAQTFIF 310 320 330 340 350 360 YAAGYETSAS TMSFLLFELA ENPDIQEKVI AEVDETLKRH NGEITYDTLS EMTYLTQVFH 370 380 390 400 410 420 ETLRKYPVAD ILLRNAKADY AVPGTNVTLK KGQTVVVSGF GIHYDPKYYP DPEKFDPERF 430 440 450 460 470 480 SPENVRNRHP CAYIPFGAGQ RKCLGMRFGQ WQVQVCIIKL LSKFRFEPST KTMSEFNYDP 490 500 KRLLVYPKSG IFLNIIPRNY

References

[1]

"Isolation and characterization of CYP6B4, a furanocoumarin-inducible cytochrome P450 from a polyphagous caterpillar (Lepidoptera: Papilionidae)." Hung C.F., Berenbaum M.R., Schuler M.A. Insect Biochem. Mol. Biol. 27:377-385(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47059 mRNA. Translation: AAB05892.1. U65489 Genomic DNA. Translation: AAB06742.1.
3D structure databases
ProteinModelPortal	Q27902.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP6B4_PAPGL

Accession

Primary (citable) accession number: Q27902
Secondary accession number(s): Q95035

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)