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Protein

TBP-related factor

Gene

Trf

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a transcription factor. Binds to the TATA box promoter element which lies close to the position of transcription initiation.
May be essential for embryonic development.

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: FlyBase
  • TFIIA-class transcription factor binding Source: FlyBase
  • TFIIB-class transcription factor binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • embryo development Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of transcription from RNA polymerase III promoter Source: FlyBase
  • RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
  • transcription from RNA polymerase III promoter Source: FlyBase
  • transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription initiation from RNA polymerase III promoter Source: FlyBase
  • transcription initiation from RNA polymerase II promoter Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
TBP-related factor
Gene namesi
Name:Trf
ORF Names:CG7562
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0010287. Trf.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
  • polytene chromosome Source: FlyBase
  • transcription factor complex Source: FlyBase
  • transcription factor TFIID complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224TBP-related factorPRO_0000153996Add
BLAST

Proteomic databases

PaxDbiQ27896.
PRIDEiQ27896.

Expressioni

Tissue specificityi

Primary spermatocytes in the adult testis and in a subset of cells in the dorsal medial region of the embryonic CNS.1 Publication

Gene expression databases

BgeeiQ27896.
GenevisibleiQ27896. DM.

Interactioni

GO - Molecular functioni

  • TFIIA-class transcription factor binding Source: FlyBase
  • TFIIB-class transcription factor binding Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi60234. 2 interactions.
MINTiMINT-1529917.
STRINGi7227.FBpp0079164.

Structurei

3D structure databases

ProteinModelPortaliQ27896.
SMRiQ27896. Positions 50-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati51 – 127771CuratedAdd
BLAST
Repeati141 – 218782CuratedAdd
BLAST

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
GeneTreeiENSGT00410000025389.
InParanoidiQ27896.
KOiK03120.
OMAiMDCLEAI.
OrthoDBiEOG7XWPPF.
PhylomeDBiQ27896.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFHFKVADA ERDRDNVAAT SNAAANPHAA LQPQQPVALV EPKDAQHEIR
60 70 80 90 100
LQNIVATFSV NCELDLKAIN SRTRNSEYSP KRFRGVIMRM HSPRCTALIF
110 120 130 140 150
RTGKVICTGA RNEIEADIGS RKFARILQKL GFPVKFMEYK LQNIVATVDL
160 170 180 190 200
RFPIRLENLN HVHGQFSSYE PEMFPGLIYR MVKPRIVLLI FVNGKVVFTG
210 220
AKSRKDIMDC LEAISPILLS FRKT
Length:224
Mass (Da):25,456
Last modified:November 1, 1996 - v1
Checksum:iCA619BE4BA726460
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70837 mRNA. Translation: CAA50185.1.
X70838 Genomic DNA. Translation: CAA50186.1.
AE014134 Genomic DNA. Translation: AAF52600.1.
AY128483 mRNA. Translation: AAM75076.1.
PIRiS37740.
RefSeqiNP_476939.1. NM_057591.4.
UniGeneiDm.3090.

Genome annotation databases

EnsemblMetazoaiFBtr0079542; FBpp0079164; FBgn0010287.
GeneIDi34102.
KEGGidme:Dmel_CG7562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70837 mRNA. Translation: CAA50185.1.
X70838 Genomic DNA. Translation: CAA50186.1.
AE014134 Genomic DNA. Translation: AAF52600.1.
AY128483 mRNA. Translation: AAM75076.1.
PIRiS37740.
RefSeqiNP_476939.1. NM_057591.4.
UniGeneiDm.3090.

3D structure databases

ProteinModelPortaliQ27896.
SMRiQ27896. Positions 50-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60234. 2 interactions.
MINTiMINT-1529917.
STRINGi7227.FBpp0079164.

Proteomic databases

PaxDbiQ27896.
PRIDEiQ27896.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079542; FBpp0079164; FBgn0010287.
GeneIDi34102.
KEGGidme:Dmel_CG7562.

Organism-specific databases

CTDi22041.
FlyBaseiFBgn0010287. Trf.

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
GeneTreeiENSGT00410000025389.
InParanoidiQ27896.
KOiK03120.
OMAiMDCLEAI.
OrthoDBiEOG7XWPPF.
PhylomeDBiQ27896.

Miscellaneous databases

GenomeRNAii34102.
PROiQ27896.

Gene expression databases

BgeeiQ27896.
GenevisibleiQ27896. DM.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new factor related to TATA-binding protein has highly restricted expression patterns in Drosophila."
    Crowley T.E., Hoey T., Liu J.-K., Jan Y.N., Jan L.Y., Tjian R.
    Nature 361:557-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.
    Strain: Canton-S.
    Tissue: Testis.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRF_DROME
AccessioniPrimary (citable) accession number: Q27896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.