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Protein

Histone H2B 2

Gene

his-4

more
Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-CEL-212300 PRC2 methylates histones and DNA
R-CEL-2299718 Condensation of Prophase Chromosomes
R-CEL-2559580 Oxidative Stress Induced Senescence
R-CEL-3214815 HDACs deacetylate histones
R-CEL-427359 SIRT1 negatively regulates rRNA expression
R-CEL-5578749 Transcriptional regulation by small RNAs
R-CEL-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-CEL-5689880 Ub-specific processing proteases
R-CEL-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-CEL-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-CEL-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B 2
Gene namesi
Name:his-4
ORF Names:T10C6.11
AND
Name:his-8
ORF Names:F45F2.12
AND
Name:his-20
ORF Names:K06C4.4
AND
Name:his-22
ORF Names:K06C4.12
AND
Name:his-52
ORF Names:F07B7.4
AND
Name:his-54
ORF Names:F07B7.11
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF07B7.11 ; CE26800 ; WBGene00001928 ; his-54
F07B7.4 ; CE10538 ; WBGene00001926 ; his-52
F45F2.12 ; CE10538 ; WBGene00001882 ; his-8
K06C4.12 ; CE10538 ; WBGene00001896 ; his-22
K06C4.4 ; CE10538 ; WBGene00001894 ; his-20
T10C6.11 ; CE10538 ; WBGene00001878 ; his-4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000718682 – 123Histone H2B 2Add BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110O-linked (GlcNAc) serineBy similarity1
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination of Lys-118 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.By similarity
GlcNAcylation at Ser-110 promotes monoubiquitination of Lys-118. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ27894
PaxDbiQ27894
PeptideAtlasiQ27894
PRIDEiQ27894

Expressioni

Gene expression databases

BgeeiWBGene00001878

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi45063, 2 interactors
56171, 1 interactor
DIPiDIP-26826N
STRINGi6239.T10C6.11

Structurei

3D structure databases

ProteinModelPortaliQ27894
SMRiQ27894
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744 Eukaryota
ENOG4111NV5 LUCA
GeneTreeiENSGT00760000118976
HOGENOMiHOG000231213
InParanoidiQ27894
KOiK11252
OMAiSHLTHYN
OrthoDBiEOG091G0XGD
PhylomeDBiQ27894

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007125 Histone_H2A/H2B/H3
IPR000558 Histone_H2B
PANTHERiPTHR23428 PTHR23428, 1 hit
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PRINTSiPR00621 HISTONEH2B
SMARTiView protein in SMART
SM00427 H2B, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00357 HISTONE_H2B, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPKPSAKG AKKAAKTVTK PKDGKKRRHA RKESYSVYIY RVLKQVHPDT
60 70 80 90 100
GVSSKAMSIM NSFVNDVFER IAAEASRLAH YNKRSTISSR EIQTAVRLIL
110 120
PGELAKHAVS EGTKAVTKYT SSK
Length:123
Mass (Da):13,572
Last modified:January 23, 2007 - v3
Checksum:iA1260A21A632940D
GO

Sequence cautioni

The sequence CCD68873 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28 – 29RH → KK AA sequence (PubMed:3753445).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081059 Genomic DNA Translation: CCD68867.2
FO081059 Genomic DNA Translation: CCD68873.1 Different initiation.
FO081551 Genomic DNA Translation: CCD72362.1
FO081551 Genomic DNA Translation: CCD72372.1
FO081223 Genomic DNA Translation: CCD70026.1
Z93388 Genomic DNA Translation: CAB07654.2
PIRiT24788
T30054 HSKW22
RefSeqiNP_505197.1, NM_072796.1
NP_505278.2, NM_072877.2
NP_505279.1, NM_072878.1
NP_505294.1, NM_072893.1
NP_505295.1, NM_072894.1
NP_507031.2, NM_074630.3
UniGeneiCel.21445
Cel.29298
Cel.3207
Cel.33086

Genome annotation databases

EnsemblMetazoaiF07B7.4; F07B7.4; WBGene00001926
F45F2.12; F45F2.12; WBGene00001882
K06C4.12; K06C4.12; WBGene00001896
K06C4.4; K06C4.4; WBGene00001894
T10C6.11; T10C6.11; WBGene00001878
GeneIDi179262
179266
180072
184117
191670
191675
KEGGicel:CELE_F07B7.11
cel:CELE_F07B7.4
cel:CELE_F45F2.12
cel:CELE_K06C4.12
cel:CELE_K06C4.4
cel:CELE_T10C6.11
UCSCiF45F2.12 c. elegans

Similar proteinsi

Entry informationi

Entry nameiH2B2_CAEEL
AccessioniPrimary (citable) accession number: Q27894
Secondary accession number(s): Q95NR2, Q9U373
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families
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