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Protein

Histone H2B 2

Gene

his-4

more
Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B 2
Gene namesi
Name:his-4
ORF Names:T10C6.11
AND
Name:his-8
ORF Names:F45F2.12
AND
Name:his-20
ORF Names:K06C4.4
AND
Name:his-22
ORF Names:K06C4.12
AND
Name:his-52
ORF Names:F07B7.4
AND
Name:his-54
ORF Names:F07B7.11
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF07B7.11; CE26800; WBGene00001928; his-54.
F07B7.4; CE26800; WBGene00001926; his-52.
F45F2.12; CE10538; WBGene00001882; his-8.
K06C4.12; CE10538; WBGene00001896; his-22.
K06C4.4; CE10538; WBGene00001894; his-20.
T10C6.11; CE16386; WBGene00001878; his-4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 123122Histone H2B 2PRO_0000071868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101O-linked (GlcNAc)By similarity
Cross-linki118 – 118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination of Lys-118 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.By similarity
GlcNAcylation at Ser-110 promotes monoubiquitination of Lys-118. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ27894.
PaxDbiQ27894.
PRIDEiQ27894.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Protein-protein interaction databases

BioGridi45063. 2 interactions.
56171. 1 interaction.
DIPiDIP-26826N.
MINTiMINT-1130213.
STRINGi6239.T10C6.11.

Structurei

3D structure databases

ProteinModelPortaliQ27894.
SMRiQ27894. Positions 2-123.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00840000129751.
HOGENOMiHOG000231213.
InParanoidiQ27894.
KOiK11252.
OrthoDBiEOG72VH8J.
PhylomeDBiQ27894.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00621. HISTONEH2B.
SMARTiSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00357. HISTONE_H2B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPKPSAKG AKKAAKTVTK PKDGKKRRHA RKESYSVYIY RVLKQVHPDT
60 70 80 90 100
GVSSKAMSIM NSFVNDVFER IAAEASRLAH YNKRSTISSR EIQTAVRLIL
110 120
PGELAKHAVS EGTKAVTKYT SSK
Length:123
Mass (Da):13,572
Last modified:January 23, 2007 - v3
Checksum:iA1260A21A632940D
GO

Sequence cautioni

The sequence CCD68873.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 292RH → KK AA sequence (PubMed:3753445).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081059 Genomic DNA. Translation: CCD68867.2.
FO081059 Genomic DNA. Translation: CCD68873.1. Different initiation.
FO081551 Genomic DNA. Translation: CCD72362.1.
FO081551 Genomic DNA. Translation: CCD72372.1.
FO081223 Genomic DNA. Translation: CCD70026.1.
Z93388 Genomic DNA. Translation: CAB07654.2.
PIRiT24788.
T30054. HSKW22.
RefSeqiNP_505197.1. NM_072796.1.
NP_505278.2. NM_072877.2.
NP_505279.1. NM_072878.1.
NP_505294.1. NM_072893.1.
NP_505295.1. NM_072894.1.
NP_507031.2. NM_074630.3.
UniGeneiCel.21445.
Cel.29298.
Cel.3207.
Cel.33086.

Genome annotation databases

EnsemblMetazoaiF07B7.4; F07B7.4; WBGene00001926.
F45F2.12; F45F2.12; WBGene00001882.
K06C4.12; K06C4.12; WBGene00001896.
K06C4.4; K06C4.4; WBGene00001894.
T10C6.11; T10C6.11; WBGene00001878.
GeneIDi179262.
179266.
180072.
184117.
191670.
191675.
KEGGicel:CELE_F07B7.11.
cel:CELE_F07B7.4.
cel:CELE_F45F2.12.
cel:CELE_K06C4.12.
cel:CELE_K06C4.4.
cel:CELE_T10C6.11.
UCSCiF45F2.12. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081059 Genomic DNA. Translation: CCD68867.2.
FO081059 Genomic DNA. Translation: CCD68873.1. Different initiation.
FO081551 Genomic DNA. Translation: CCD72362.1.
FO081551 Genomic DNA. Translation: CCD72372.1.
FO081223 Genomic DNA. Translation: CCD70026.1.
Z93388 Genomic DNA. Translation: CAB07654.2.
PIRiT24788.
T30054. HSKW22.
RefSeqiNP_505197.1. NM_072796.1.
NP_505278.2. NM_072877.2.
NP_505279.1. NM_072878.1.
NP_505294.1. NM_072893.1.
NP_505295.1. NM_072894.1.
NP_507031.2. NM_074630.3.
UniGeneiCel.21445.
Cel.29298.
Cel.3207.
Cel.33086.

3D structure databases

ProteinModelPortaliQ27894.
SMRiQ27894. Positions 2-123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45063. 2 interactions.
56171. 1 interaction.
DIPiDIP-26826N.
MINTiMINT-1130213.
STRINGi6239.T10C6.11.

Proteomic databases

EPDiQ27894.
PaxDbiQ27894.
PRIDEiQ27894.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF07B7.4; F07B7.4; WBGene00001926.
F45F2.12; F45F2.12; WBGene00001882.
K06C4.12; K06C4.12; WBGene00001896.
K06C4.4; K06C4.4; WBGene00001894.
T10C6.11; T10C6.11; WBGene00001878.
GeneIDi179262.
179266.
180072.
184117.
191670.
191675.
KEGGicel:CELE_F07B7.11.
cel:CELE_F07B7.4.
cel:CELE_F45F2.12.
cel:CELE_K06C4.12.
cel:CELE_K06C4.4.
cel:CELE_T10C6.11.
UCSCiF45F2.12. c. elegans.

Organism-specific databases

CTDi179262.
179266.
180072.
184117.
191670.
191675.
WormBaseiF07B7.11; CE26800; WBGene00001928; his-54.
F07B7.4; CE26800; WBGene00001926; his-52.
F45F2.12; CE10538; WBGene00001882; his-8.
K06C4.12; CE10538; WBGene00001896; his-22.
K06C4.4; CE10538; WBGene00001894; his-20.
T10C6.11; CE16386; WBGene00001878; his-4.

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00840000129751.
HOGENOMiHOG000231213.
InParanoidiQ27894.
KOiK11252.
OrthoDBiEOG72VH8J.
PhylomeDBiQ27894.

Miscellaneous databases

PROiQ27894.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00621. HISTONEH2B.
SMARTiSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00357. HISTONE_H2B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Multiple forms of histone H2B from the nematode Caenorhabditis elegans."
    Vanfleteren J.R., van Bun S.M., Delcambe L.L., van Beeumen J.J.
    Biochem. J. 235:769-773(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-123.

Entry informationi

Entry nameiH2B2_CAEEL
AccessioniPrimary (citable) accession number: Q27894
Secondary accession number(s): Q95NR2, Q9U373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.