Q27893 (NTP1_TOXGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside-triphosphatase 1
EC=3.6.1.15

Alternative name(s):
NTPase-I
Nucleoside triphosphate hydrolase 1
Nucleoside-triphosphatase I

Gene names

Name:

NTP3

Organism

Toxoplasma gondii

Taxonomic identifier

5811 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Coccidia › Eucoccidiorida › Eimeriorina › Sarcocystidae › Toxoplasma

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May perform an important processing step in the conversion of high energy nucleotides prior to uptake by the parasite and may contribute to intracellular survival and virulence. NTPAse-I has a specific activity 4.5-fold higher than NTPAse-II in hydrolysis of ATP. The primary difference between these isozymes lies in their ability to hydrolyze nucleoside triphosphate versus diphosphate substrates. While NTPAse-II hydrolyzes ATP to ADP and ADP to AMP at almost the same rate, NTPAse-I hydrolyzes ADP to AMP at a much slower rate (0.7% of the rate for ATP).
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Homotetramer.
Subcellular location	Secreted. Parasitophorous vacuole. Note: Found in host cell parasitophorous vacuole.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	symbiont-containing vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleoside-triphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Chain	26 – 628	603	Nucleoside-triphosphatase 1		PRO_0000019911
Sites
	Active site	236	1	Proton acceptor By similarity
Amino acid modifications
	Glycosylation	432	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q27893 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 95867B943ADD3E6F	FASTA	628	69,159
	10 20 30 40 50 60 MWLPVYVPLL LVFGVSLSLP QGSLGTDSSS LRGVDADTEK RINVGKKHLQ TLRNLETRCH 70 80 90 100 110 120 DSLQALVVID AGSSSTRTNV FLAKTRSCPN KGRSIDPDSI QLIGAGKRFA GLRVVLEEWL 130 140 150 160 170 180 DTYAGKDWES RPVDARLLFQ YVPQMHEGAK KLMQLLEEDT VAILDSQLNE KQKVQVKALG 190 200 210 220 230 240 IPVMLCSTAG VRDFHEWYRD ALFVLLRHLI NNPSPAHGYK FFTNPFWTRP ITGAEEGLFA 250 260 270 280 290 300 FITLNHLSRR LGEDPARCMI DEYGVKQCRN DLAGVVEVGG ASAQIVFPLQ EGTVLPSSVR 310 320 330 340 350 360 AVNLQRERLL PERYPSADVV SVSFMQLGMA SSAGLFLKEL CSNDEFLQGG ICSNPCLFKG 370 380 390 400 410 420 FQQSCSAGEV EVRPDGSASV NEDVRKNRLK PLATYCSVNN PEISFKVTNE MQCRENSIDP 430 440 450 460 470 480 TKPLAERMKI ENCSIIKGTG NFDKCVSQVE SILVAPKLPL PANIEAASSG FESVDQVFRF 490 500 510 520 530 540 ASSTAPMIVT GGGMLAAINT LKDHRLLRSD FSGDVEELAE AAREFCSSEV IIRTDGPVIQ 550 560 570 580 590 600 LPNARGEQKL NSLNFDLCKT MALTVSLLRH MAAGENQPSF IKWEKSIAGP DGKPLADLGW 610 620 QVGVILHHVL FTEEWGRNAY EAGYSHNL

References

[1]	"Biochemical and molecular characterization of nucleoside triphosphate hydrolase isozymes from the parasitic protozoan Toxoplasma gondii." Asai T., Miura S., Sibley L.D., Okabayashi H., Takeuchi T. J. Biol. Chem. 270:11391-11397(1995) [PubMed: 7744775] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: RH.
[2]	"Tandemly repeated genes encode nucleoside triphosphate hydrolase isoforms secreted into the parasitophorous vacuole of Toxoplasma gondii." Bermudes D., Peck K.R., Afifi M.A., Beckers C.J.M., Joiner K.A. J. Biol. Chem. 269:29252-29260(1994) [PubMed: 7961894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: RH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L39078 mRNA. Translation: AAA89203.1.
U96965 Genomic DNA. Translation: AAC80188.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4A57	X-ray	2.00	A/B/C/D	26-628	[»]
4A59	X-ray	2.20	A/B/C/D	26-628	[»]
4A5A	X-ray	2.85	A/B/C/D	26-628	[»]

ProteinModelPortal

Q27893.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR000407. GDA1_CD39_NTPase.
IPR017227. NTPase_alveloata.
[Graphical view]

Pfam

PF01150. GDA1_CD39. 1 hit.
[Graphical view]

PIRSF

PIRSF037506. NTPase. 1 hit.

PROSITE

PS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

NTP1_TOXGO

Accession

Primary (citable) accession number: Q27893

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)