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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit 2

Gene

Pp2B-14D

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi156IronBy similarity1
Metal bindingi158IronBy similarity1
Metal bindingi184IronBy similarity1
Metal bindingi184ZincBy similarity1
Metal bindingi216ZincBy similarity1
Active sitei217Proton donorBy similarity1
Metal bindingi265ZincBy similarity1
Metal bindingi347ZincBy similarity1

GO - Molecular functioni

  • calcium-dependent protein serine/threonine phosphatase activity Source: FlyBase
  • calmodulin binding Source: FlyBase
  • calmodulin-dependent protein phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: FlyBase

GO - Biological processi

  • female meiotic nuclear division Source: FlyBase
  • meiotic cell cycle Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • regulation of embryonic development Source: FlyBase
  • sleep Source: FlyBase
  • wing disc development Source: FlyBase

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase, Protein phosphatase
LigandCalcium, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-2025928 Calcineurin activates NFAT
R-DME-2871809 FCERI mediated Ca+2 mobilization
R-DME-4086398 Ca2+ pathway
R-DME-5607763 CLEC7A (Dectin-1) induces NFAT activation
SignaLinkiQ27889

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit 2 (EC:3.1.3.16)
Alternative name(s):
Calmodulin-dependent calcineurin A2 subunit
Gene namesi
ORF Names:CG9842
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0011826 Pp2B-14D

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588311 – 570Serine/threonine-protein phosphatase 2B catalytic subunit 2Add BLAST570

Proteomic databases

PaxDbiQ27889
PRIDEiQ27889

Expressioni

Tissue specificityi

Expressed in CNS and PNS.1 Publication

Developmental stagei

Expressed both maternally and zygotically in embryos, larvae and adults.2 Publications

Gene expression databases

BgeeiFBgn0011826
ExpressionAtlasiQ27889 baseline and differential
GenevisibleiQ27889 DM

Interactioni

Subunit structurei

Interacts with sra in a complex that contains CanA-14F.2 Publications

GO - Molecular functioni

  • calmodulin binding Source: FlyBase

Protein-protein interaction databases

BioGridi58962, 41 interactors
DIPiDIP-17529N
IntActiQ27889, 15 interactors
STRINGi7227.FBpp0301546

Structurei

3D structure databases

ProteinModelPortaliQ27889
SMRiQ27889
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 40Poly-Asn5

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiKOG0375 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00530000063087
InParanoidiQ27889
KOiK04348
OMAiQFDVKVG
OrthoDBiEOG091G094R
PhylomeDBiQ27889

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q27889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNNQSSSV AQAATSARTV SAGSAEATDA NSTASNNNNN SSSTAAAGNN
60 70 80 90 100
SDNSSPTTGT GTGASTGKLH GGHTAVNTKE RVVDSVPFPP SHKLTLAEVF
110 120 130 140 150
DQRTGKPNHE LLKQHFILEG RIEEAPALKI IQDGAALLRQ EKTMIDIEAP
160 170 180 190 200
VTVCGDIHGQ FYDLMKLFEV GGSPASTKYL FLGDYVDRGY FSIECVLYLW
210 220 230 240 250
SLKITYPQTL FLLRGNHECR HLTEYFTFKQ ECKIKYSERV YDACMDAFDC
260 270 280 290 300
LPLAALMNQQ FLCVHGGLSP EIHELEDIRR LDRFKEPPAF GPMCDLLWSD
310 320 330 340 350
PLEDFGNEKN SDFYTHNSVR GCSYFYSYAA CCDFLQNNNL LSIIRAHEAQ
360 370 380 390 400
DAGYRMYRKS QTTGFPSLIT IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF
410 420 430 440 450
NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM LVNVLNICSD DELMTEESEE
460 470 480 490 500
PLSDDEAALR KEVIRNKIRA IGKMARVFSV LREESESVLQ LKGLTPTGAL
510 520 530 540 550
PLGALSGGKQ SLKNAMQGFS PNHKITSFAE AKGLDAVNER MPPRRDQPPT
560 570
PSEDPNQHSQ QGGKNGAGHG
Length:570
Mass (Da):63,101
Last modified:August 30, 2005 - v2
Checksum:i4A5429A620C0C275
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111Missing (PubMed:8849894).Curated1
Sequence conflicti133D → E in CAA54807 (PubMed:8313960).Curated1
Sequence conflicti175A → Q in CAA54807 (PubMed:8313960).Curated1
Sequence conflicti175A → Q in AAB29893 (PubMed:8849894).Curated1
Sequence conflicti323 – 333SYFYSYAACCD → CY in CAA54807 (PubMed:8313960).CuratedAdd BLAST11
Sequence conflicti407Y → I in CAA54807 (PubMed:8313960).Curated1
Sequence conflicti459L → V in CAA54807 (PubMed:8313960).Curated1
Sequence conflicti491Missing in CAA54807 (PubMed:8313960).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77768 mRNA Translation: CAA54807.1
S68806 mRNA Translation: AAB29893.1
AE014298 Genomic DNA Translation: AAF48622.1
BT010083 mRNA Translation: AAQ22552.1
PIRiS41743
S70551
RefSeqiNP_001245715.1, NM_001258786.3
NP_001245716.1, NM_001258787.2
NP_523373.2, NM_078649.4
UniGeneiDm.33340

Genome annotation databases

EnsemblMetazoaiFBtr0074294; FBpp0074069; FBgn0011826
FBtr0305593; FBpp0294044; FBgn0011826
FBtr0309793; FBpp0301546; FBgn0011826
GeneIDi32624
KEGGidme:Dmel_CG9842

Similar proteinsi

Entry informationi

Entry nameiPP2B2_DROME
AccessioniPrimary (citable) accession number: Q27889
Secondary accession number(s): Q26248, Q9VXF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 30, 2005
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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