Reviewed, UniProtKB/Swiss-Prot Q27889 (PP2B2_DROME)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit 2
    EC=3.1.3.16
Alternative name(s):
    Calmodulin-dependent calcineurin A2 subunit

Gene names

Name:	Pp2B-14D
ORF Names:	CG9842

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
Catalytic activity	A phosphoprotein + H(2)O = a protein + phosphate.
Cofactor	Binds 1 Fe(3+) ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with sra in a complex that contains CanA-14F.
Tissue specificity	Expressed in CNS and PNS.
Developmental stage	Expressed both maternally and zygotically in embryos, larvae and adults.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2B subfamily.

Ontologies

Keywords
Ligand	Calcium Calmodulin-binding Iron Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	female meiosis Ref.6 Inferred from mutant phenotype. Source: FlyBase protein amino acid dephosphorylation Non-traceable author statement. Source: FlyBase wing disc development Ref.6 Inferred from mutant phenotype. Source: FlyBase
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein phosphatase activity Ref.1 Inferred from mutant phenotype. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9W1K5-1	1	EBI-132663,EBI-212772
CG12934	Q9V5N7	1	EBI-132663,EBI-181534
CG17159	Q8SYH8	1	EBI-132663,EBI-133012
hoe1	Q9VR47	1	EBI-132663,EBI-111149
sra	Q9XZL8	1	EBI-132663,EBI-147402

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Serine/threonine-protein phosphatase 2B catalytic subunit 2		PRO_0000058831
Regions
	Compositional bias	36 – 40	5	Poly-Asn
Sites
	Active site	217	1	Proton donor By similarity
	Metal binding	156	1	Iron By similarity
	Metal binding	158	1	Iron By similarity
	Metal binding	184	1	Iron By similarity
	Metal binding	184	1	Zinc By similarity
	Metal binding	216	1	Zinc By similarity
	Metal binding	265	1	Zinc By similarity
	Metal binding	347	1	Zinc By similarity
Experimental info
	Sequence conflict	111	1	Missing Ref.2
	Sequence conflict	133	1	D → E in CAA54807. Ref.1
	Sequence conflict	175	1	A → Q in CAA54807. Ref.1
	Sequence conflict	175	1	A → Q in AAB29893. Ref.2
	Sequence conflict	323 – 333	11	SYFYSYAACCD → CY in CAA54807. Ref.1
	Sequence conflict	407	1	Y → I in CAA54807. Ref.1
	Sequence conflict	459	1	L → V in CAA54807. Ref.1
	Sequence conflict	491	1	Missing in CAA54807. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q27889-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 4A5429A620C0C275	FASTA	570	63,101
	10 20 30 40 50 60 MSSNNQSSSV AQAATSARTV SAGSAEATDA NSTASNNNNN SSSTAAAGNN SDNSSPTTGT 70 80 90 100 110 120 GTGASTGKLH GGHTAVNTKE RVVDSVPFPP SHKLTLAEVF DQRTGKPNHE LLKQHFILEG 130 140 150 160 170 180 RIEEAPALKI IQDGAALLRQ EKTMIDIEAP VTVCGDIHGQ FYDLMKLFEV GGSPASTKYL 190 200 210 220 230 240 FLGDYVDRGY FSIECVLYLW SLKITYPQTL FLLRGNHECR HLTEYFTFKQ ECKIKYSERV 250 260 270 280 290 300 YDACMDAFDC LPLAALMNQQ FLCVHGGLSP EIHELEDIRR LDRFKEPPAF GPMCDLLWSD 310 320 330 340 350 360 PLEDFGNEKN SDFYTHNSVR GCSYFYSYAA CCDFLQNNNL LSIIRAHEAQ DAGYRMYRKS 370 380 390 400 410 420 QTTGFPSLIT IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL 430 440 450 460 470 480 PFVGEKVTEM LVNVLNICSD DELMTEESEE PLSDDEAALR KEVIRNKIRA IGKMARVFSV 490 500 510 520 530 540 LREESESVLQ LKGLTPTGAL PLGALSGGKQ SLKNAMQGFS PNHKITSFAE AKGLDAVNER 550 560 570 MPPRRDQPPT PSEDPNQHSQ QGGKNGAGHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cDNA encoding a Drosophila calcium/calmodulin regulated protein phosphatase, which has its most abundant expression in the early embryo." Brown L., Chen M.X., Cohen P.T.W. FEBS Lett. 339:124-128(1994) [PubMed: 8313960] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Strain: Oregon-R. Tissue: Eye imaginal disk.
[2]	"Molecular characterization of neurally expressing genes in the para sodium channel gene cluster of Drosophila." Hong C.-S., Ganetzky B. Genetics 142:879-892(1996) [PubMed: 8849894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"The calcineurin regulator sra plays an essential role in female meiosis in Drosophila." Takeo S., Tsuda M., Akahori S., Matsuo T., Aigaki T. Curr. Biol. 16:1435-1440(2006) [PubMed: 16860743] [Abstract] Cited for: FUNCTION, INTERACTION WITH SRA.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77768 mRNA. Translation: CAA54807.1. S68806 mRNA. Translation: AAB29893.1. AE014298 Genomic DNA. Translation: AAF48622.1. BT010083 mRNA. Translation: AAQ22552.1.
PIR	S41743. S70551.
RefSeq	NP_523373.2.
UniGene	Dm.33340
3D structure databases
HSSP	HSSP built from PDB template 1AUI based on UniProtKB Q08209.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q27889.
Genome annotation databases
Ensembl	CG9842. Drosophila melanogaster. [Contig view]
GeneID	32624.
KEGG	dme:Dmel_CG9842.
NMPDR	fig|7227.3.peg.18200.
Organism-specific databases
FlyBase	FBgn0011826. Pp2B-14D.
Phylogenomic databases
HOGENOM	Q27889.
Gene expression databases
ArrayExpress	Q27889.
GermOnline	CG9842. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR006186. T_phtase_apaH. [Graphical view]
PANTHER	PTHR11668. T_phtase_apaH. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
ProDom	PD000252. T_phtase_apaH. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	779520.

Entry information

Entry name

PP2B2_DROME

Accession

Primary (citable) accession number: Q27889
Secondary accession number(s): Q26248, Q9VXF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 30, 2005
Last modified:	November 25, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project