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Q27889

- PP2B2_DROME

UniProt

Q27889 - PP2B2_DROME

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Protein
Serine/threonine-protein phosphatase 2B catalytic subunit 2
Gene
Pp2B-14D, CG9842
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 1 Fe3+ ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi156 – 1561Iron By similarity
Metal bindingi158 – 1581Iron By similarity
Metal bindingi184 – 1841Iron By similarity
Metal bindingi184 – 1841Zinc By similarity
Metal bindingi216 – 2161Zinc By similarity
Active sitei217 – 2171Proton donor By similarity
Metal bindingi265 – 2651Zinc By similarity
Metal bindingi347 – 3471Zinc By similarity

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: FlyBase
  2. calmodulin binding Source: FlyBase
  3. calmodulin-dependent protein phosphatase activity Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: UniProtKB
  6. protein serine/threonine phosphatase activity Source: FlyBase

GO - Biological processi

  1. female meiotic division Source: FlyBase
  2. meiotic nuclear division Source: FlyBase
  3. protein dephosphorylation Source: FlyBase
  4. sleep Source: FlyBase
  5. wing disc development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calcium, Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_184321. Ca2+ pathway.
REACT_203584. FCERI mediated Ca+2 mobilization.
REACT_213958. DARPP-32 events.
SignaLinkiQ27889.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit 2 (EC:3.1.3.16)
Alternative name(s):
Calmodulin-dependent calcineurin A2 subunit
Gene namesi
Name:Pp2B-14D
ORF Names:CG9842
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0011826. Pp2B-14D.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Serine/threonine-protein phosphatase 2B catalytic subunit 2
PRO_0000058831Add
BLAST

Proteomic databases

PaxDbiQ27889.
PRIDEiQ27889.

Expressioni

Tissue specificityi

Expressed in CNS and PNS.1 Publication

Developmental stagei

Expressed both maternally and zygotically in embryos, larvae and adults.2 Publications

Gene expression databases

BgeeiQ27889.

Interactioni

Subunit structurei

Interacts with sra in a complex that contains CanA-14F.2 Publications

Protein-protein interaction databases

BioGridi58962. 33 interactions.
DIPiDIP-17529N.
IntActiQ27889. 10 interactions.
MINTiMINT-809182.

Structurei

3D structure databases

ProteinModelPortaliQ27889.
SMRiQ27889. Positions 86-479.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi36 – 405Poly-Asn

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
InParanoidiQ27889.
KOiK04348.
OMAiMFWSPED.
OrthoDBiEOG7BZVSC.
PhylomeDBiQ27889.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27889-1 [UniParc]FASTAAdd to Basket

« Hide

MSSNNQSSSV AQAATSARTV SAGSAEATDA NSTASNNNNN SSSTAAAGNN    50
SDNSSPTTGT GTGASTGKLH GGHTAVNTKE RVVDSVPFPP SHKLTLAEVF 100
DQRTGKPNHE LLKQHFILEG RIEEAPALKI IQDGAALLRQ EKTMIDIEAP 150
VTVCGDIHGQ FYDLMKLFEV GGSPASTKYL FLGDYVDRGY FSIECVLYLW 200
SLKITYPQTL FLLRGNHECR HLTEYFTFKQ ECKIKYSERV YDACMDAFDC 250
LPLAALMNQQ FLCVHGGLSP EIHELEDIRR LDRFKEPPAF GPMCDLLWSD 300
PLEDFGNEKN SDFYTHNSVR GCSYFYSYAA CCDFLQNNNL LSIIRAHEAQ 350
DAGYRMYRKS QTTGFPSLIT IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF 400
NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM LVNVLNICSD DELMTEESEE 450
PLSDDEAALR KEVIRNKIRA IGKMARVFSV LREESESVLQ LKGLTPTGAL 500
PLGALSGGKQ SLKNAMQGFS PNHKITSFAE AKGLDAVNER MPPRRDQPPT 550
PSEDPNQHSQ QGGKNGAGHG 570
Length:570
Mass (Da):63,101
Last modified:August 30, 2005 - v2
Checksum:i4A5429A620C0C275
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111Missing1 Publication
Sequence conflicti133 – 1331D → E in CAA54807. 1 Publication
Sequence conflicti175 – 1751A → Q in CAA54807. 1 Publication
Sequence conflicti175 – 1751A → Q in AAB29893. 1 Publication
Sequence conflicti323 – 33311SYFYSYAACCD → CY in CAA54807. 1 Publication
Add
BLAST
Sequence conflicti407 – 4071Y → I in CAA54807. 1 Publication
Sequence conflicti459 – 4591L → V in CAA54807. 1 Publication
Sequence conflicti491 – 4911Missing in CAA54807. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77768 mRNA. Translation: CAA54807.1.
S68806 mRNA. Translation: AAB29893.1.
AE014298 Genomic DNA. Translation: AAF48622.1.
BT010083 mRNA. Translation: AAQ22552.1.
PIRiS41743.
S70551.
RefSeqiNP_001245715.1. NM_001258786.2.
NP_001245716.1. NM_001258787.1.
NP_523373.2. NM_078649.3.
UniGeneiDm.33340.

Genome annotation databases

EnsemblMetazoaiFBtr0074294; FBpp0074069; FBgn0011826.
FBtr0305593; FBpp0294044; FBgn0011826.
FBtr0309793; FBpp0301546; FBgn0011826.
GeneIDi32624.
KEGGidme:Dmel_CG9842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77768 mRNA. Translation: CAA54807.1 .
S68806 mRNA. Translation: AAB29893.1 .
AE014298 Genomic DNA. Translation: AAF48622.1 .
BT010083 mRNA. Translation: AAQ22552.1 .
PIRi S41743.
S70551.
RefSeqi NP_001245715.1. NM_001258786.2.
NP_001245716.1. NM_001258787.1.
NP_523373.2. NM_078649.3.
UniGenei Dm.33340.

3D structure databases

ProteinModelPortali Q27889.
SMRi Q27889. Positions 86-479.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 58962. 33 interactions.
DIPi DIP-17529N.
IntActi Q27889. 10 interactions.
MINTi MINT-809182.

Proteomic databases

PaxDbi Q27889.
PRIDEi Q27889.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0074294 ; FBpp0074069 ; FBgn0011826 .
FBtr0305593 ; FBpp0294044 ; FBgn0011826 .
FBtr0309793 ; FBpp0301546 ; FBgn0011826 .
GeneIDi 32624.
KEGGi dme:Dmel_CG9842.

Organism-specific databases

CTDi 32624.
FlyBasei FBgn0011826. Pp2B-14D.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00530000063087.
InParanoidi Q27889.
KOi K04348.
OMAi MFWSPED.
OrthoDBi EOG7BZVSC.
PhylomeDBi Q27889.

Enzyme and pathway databases

Reactomei REACT_184321. Ca2+ pathway.
REACT_203584. FCERI mediated Ca+2 mobilization.
REACT_213958. DARPP-32 events.
SignaLinki Q27889.

Miscellaneous databases

ChiTaRSi Pp2B-14D. drosophila.
GenomeRNAii 32624.
NextBioi 779520.

Gene expression databases

Bgeei Q27889.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a cDNA encoding a Drosophila calcium/calmodulin regulated protein phosphatase, which has its most abundant expression in the early embryo."
    Brown L., Chen M.X., Cohen P.T.W.
    FEBS Lett. 339:124-128(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
    Tissue: Eye imaginal disk.
  2. "Molecular characterization of neurally expressing genes in the para sodium channel gene cluster of Drosophila."
    Hong C.-S., Ganetzky B.
    Genetics 142:879-892(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  6. "The calcineurin regulator sra plays an essential role in female meiosis in Drosophila."
    Takeo S., Tsuda M., Akahori S., Matsuo T., Aigaki T.
    Curr. Biol. 16:1435-1440(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SRA.
  7. "Shaggy/glycogen synthase kinase 3beta and phosphorylation of Sarah/regulator of calcineurin are essential for completion of Drosophila female meiosis."
    Takeo S., Swanson S.K., Nandanan K., Nakai Y., Aigaki T., Washburn M.P., Florens L., Hawley R.S.
    Proc. Natl. Acad. Sci. U.S.A. 109:6382-6389(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRA.

Entry informationi

Entry nameiPP2B2_DROME
AccessioniPrimary (citable) accession number: Q27889
Secondary accession number(s): Q26248, Q9VXF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 30, 2005
Last modified: September 3, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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