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Protein

Enolase

Gene

ENO

Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Smp_056970.3), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (Smp_056970.1), Glyceraldehyde-3-phosphate dehydrogenase (Smp_056970.4), Glyceraldehyde-3-phosphate dehydrogenase (Smp_056970.2)
  2. Phosphoglycerate kinase (PGK)
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. Pyruvate kinase (Smp_065610.1), Pyruvate kinase (Smp_065610.2), Pyruvate kinase (Smp_156360)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei158SubstrateBy similarity1
Binding sitei167SubstrateBy similarity1
Active sitei210Proton donorBy similarity1
Metal bindingi245MagnesiumBy similarity1
Metal bindingi294MagnesiumBy similarity1
Binding sitei294SubstrateBy similarity1
Metal bindingi319MagnesiumBy similarity1
Binding sitei319SubstrateBy similarity1
Active sitei344Proton acceptorBy similarity1
Binding sitei395SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340841 – 434EnolaseAdd BLAST434

Proteomic databases

PRIDEiQ27877.

Expressioni

Gene expression databases

ExpressionAtlasiQ27877. baseline.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi6183.Smp_024110__mRNA.

Structurei

3D structure databases

ProteinModelPortaliQ27877.
SMRiQ27877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni371 – 374Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
OrthoDBiEOG091G07NH.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILTIHARQ IFDSRGNPTV EVDLKTSKGL FRAAVPSGAS TGVHEALELR
60 70 80 90 100
DTNSKAYMKK GVLTAVSNVN KIIAPALINK NIPVTNQAAI DKYMIDLDGT
110 120 130 140 150
ENKEKLGANA ILGVSLAVCK AGAAEAGLPL YRYIARLAGH EDVIMPVPAF
160 170 180 190 200
NVINGGSHAG NKLAMQEFMI LPTGASSFTE AMQIGTEVYH NLKAVIKREY
210 220 230 240 250
GLDACNVGDE GGFAPNIQDN MKGLQLLEEA IKIAGYTGKV EIGMDCAASE
260 270 280 290 300
FHKNGKYDLD FKNPHSAEST WLSPDAMANM YKQMISKFPI VSIEDPFDQD
310 320 330 340 350
DWETWPKLTS STNIQIVGDD LTVTNPKRIK QAIASKACNC LLLKVNQIGS
360 370 380 390 400
LTESIEACKL AQDSGWGVMV SHRSGETEDT FIADLVVGLC TGQIKTGAPC
410 420 430
RSDRLAKYNQ LLRIEEELGT AAKYAGKNFR HPKV
Length:434
Mass (Da):46,995
Last modified:November 1, 1996 - v1
Checksum:i5318C52F7EDFE5EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33177, U30177 Genomic DNA. Translation: AAC46884.1.
U30175 mRNA. Translation: AAC46886.1.

Genome annotation databases

GeneDBiSmp_024110.1:pep.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33177, U30177 Genomic DNA. Translation: AAC46884.1.
U30175 mRNA. Translation: AAC46886.1.

3D structure databases

ProteinModelPortaliQ27877.
SMRiQ27877.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6183.Smp_024110__mRNA.

Proteomic databases

PRIDEiQ27877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneDBiSmp_024110.1:pep.

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
OrthoDBiEOG091G07NH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Gene expression databases

ExpressionAtlasiQ27877. baseline.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_SCHMA
AccessioniPrimary (citable) accession number: Q27877
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 12, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.