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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. This enzyme does not act on D-aspartyl residues.

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601By similarity

GO - Molecular functioni

  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: WormBase

GO - Biological processi

  • multicellular organismal response to stress Source: WormBase
  • peptidyl-aspartic acid modification Source: WormBase
  • protein methylation Source: WormBase
  • regulation of autophagy Source: WormBase
  • response to starvation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-CEL-5676934. Protein repair.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:pcm-1
ORF Names:C10F3.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC10F3.5a; CE08070; WBGene00003954; pcm-1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: WormBase
  • cytoplasm Source: WormBase
  • cytosol Source: WormBase
  • neuronal cell body Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Protein-L-isoaspartate O-methyltransferasePRO_0000111880Add
BLAST

Proteomic databases

EPDiQ27873.
PaxDbiQ27873.

Expressioni

Gene expression databases

BgeeiWBGene00003954.
ExpressionAtlasiQ27873. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi44029. 1 interaction.
DIPiDIP-25593N.
MINTiMINT-1065793.
STRINGi6239.C10F3.5a.

Structurei

3D structure databases

ProteinModelPortaliQ27873.
SMRiQ27873. Positions 3-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
GeneTreeiENSGT00510000046974.
HOGENOMiHOG000257189.
InParanoidiQ27873.
OMAiANNEDLI.
OrthoDBiEOG091G0OTG.
PhylomeDBiQ27873.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWRSSGSTN SELIDNLRNN RVFASQRAYD AMKSVDRGDF APRAPYEDAP
60 70 80 90 100
QRIGYNATVS APHMHAAALD YLQNHLVAGA KALDVGSGSG YLTVCMAMMV
110 120 130 140 150
GRNGTVVGIE HMPQLVELSE KNIRKHHSEQ LERGNVIIIE GDGRQGFAEK
160 170 180 190 200
APYNAIHVGA ASKGVPKALT DQLAEGGRMM IPVEQVDGNQ VFMQIDKING
210 220
KIEQKIVEHV IYVPLTSREE QWNRN
Length:225
Mass (Da):24,767
Last modified:November 1, 1996 - v1
Checksum:i004E7D7BBF9D3B85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15129 Genomic DNA. Translation: AAB60240.1.
U09669 mRNA. Translation: AAA82166.1.
FO080493 Genomic DNA. Translation: CCD64125.1.
PIRiT32150.
RefSeqiNP_504551.3. NM_072150.5.
UniGeneiCel.17987.

Genome annotation databases

EnsemblMetazoaiC10F3.5a; C10F3.5a; WBGene00003954.
GeneIDi178981.
UCSCiC10F3.5a. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15129 Genomic DNA. Translation: AAB60240.1.
U09669 mRNA. Translation: AAA82166.1.
FO080493 Genomic DNA. Translation: CCD64125.1.
PIRiT32150.
RefSeqiNP_504551.3. NM_072150.5.
UniGeneiCel.17987.

3D structure databases

ProteinModelPortaliQ27873.
SMRiQ27873. Positions 3-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44029. 1 interaction.
DIPiDIP-25593N.
MINTiMINT-1065793.
STRINGi6239.C10F3.5a.

Proteomic databases

EPDiQ27873.
PaxDbiQ27873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC10F3.5a; C10F3.5a; WBGene00003954.
GeneIDi178981.
UCSCiC10F3.5a. c. elegans.

Organism-specific databases

CTDi178981.
WormBaseiC10F3.5a; CE08070; WBGene00003954; pcm-1.

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
GeneTreeiENSGT00510000046974.
HOGENOMiHOG000257189.
InParanoidiQ27873.
OMAiANNEDLI.
OrthoDBiEOG091G0OTG.
PhylomeDBiQ27873.

Enzyme and pathway databases

ReactomeiR-CEL-5676934. Protein repair.

Miscellaneous databases

PROiQ27873.

Gene expression databases

BgeeiWBGene00003954.
ExpressionAtlasiQ27873. baseline and differential.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT_CAEEL
AccessioniPrimary (citable) accession number: Q27873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.