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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

GSPATT00019973001

Organism
Paramecium tetraurelia
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (GSPATT00019973001)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Substrate; via carbonyl oxygenBy similarity1
Binding sitei12NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei32SubstrateBy similarity1
Binding sitei101Substrate; via carbonyl oxygenBy similarity1
Binding sitei122SubstrateBy similarity1
Binding sitei200dUMPBy similarity1
Active sitei345By similarity1
Binding sitei346dUMPBy similarity1
Binding sitei376dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 24NADPBy similarity7
Nucleotide bindingi49 – 51NADPBy similarity3
Nucleotide bindingi68 – 71NADPBy similarity4
Nucleotide bindingi102 – 109NADPBy similarity8
Nucleotide bindingi364 – 368dUMPBy similarity5
Nucleotide bindingi406 – 408dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processNucleotide biosynthesis, One-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
ORF Names:GSPATT00019973001
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
Proteomesi
  • UP000000600 Componenti: Partially assembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863461 – 462Bifunctional dihydrofolate reductase-thymidylate synthaseAdd BLAST462

Proteomic databases

PRIDEiQ27828

Interactioni

Protein-protein interaction databases

STRINGi412030.XP_001453677.1

Structurei

3D structure databases

ProteinModelPortaliQ27828
SMRiQ27828
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 165DHFRAdd BLAST160

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 462Thymidylate synthaseAdd BLAST283

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673 Eukaryota
KOG1324 Eukaryota
COG0207 LUCA
COG0262 LUCA
InParanoidiQ27828
KOiK13998
OMAiVYSNHVE

Family and domain databases

CDDicd00209 DHFR, 1 hit
cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.430.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR012262 DHFR-TS
IPR017925 DHFR_CS
IPR001796 DHFR_dom
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PANTHERiPTHR11548:SF7 PTHR11548:SF7, 2 hits
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PF00303 Thymidylat_synt, 1 hit
PIRSFiPIRSF000389 DHFR-TS, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF53597 SSF53597, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q27828-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTNKTFSMV LAMTLNGGIG YQNRLPWKLK EDLQRFKKIT TGGIVIMGRK
60 70 80 90 100
TFESMNSKPL PNRVNVVISK NMKSSNEVQV FPRIEDALQF YNTSHQKLYL
110 120 130 140 150
IGGKRIFEEG LATDKCSDVH LTRIGVETKC DVYLNKNIFS TFQVNKTSQT
160 170 180 190 200
KSENGINYDY QHLINKNSHE QSYIDEEHQE NQYLDMITKI MKEGVSKDDR
210 220 230 240 250
TGVGTMSIFG QTMRFNLAQS FPLLTTKKVF FRGVVEELLW FLRGNTNGKL
260 270 280 290 300
LLDKGVKIWE GNGTREYLDT IGLQHRQEHD LGPVYGFQWR HFGAKYKDCQ
310 320 330 340 350
TDYSNQGVDQ VKEIIQLLKN NPDSRRIILS AWNPIDLKQM ALPPCHVMSQ
360 370 380 390 400
FFVANGKLSC MMYQRSCDFG LGIPFNIASY ALLTYMLAKE CNLNLGEFVH
410 420 430 440 450
VLGDTHIYSN HVEALKKQIE RVPYPFPLLK IKGNKSLFDY TYEDFELVGY
460
NAHDKIEMKM AV
Length:462
Mass (Da):53,201
Last modified:November 1, 1996 - v1
Checksum:iC8C9B3A12BBD5C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03885 Genomic DNA Translation: AAC47025.1
CT868563 Genomic DNA Translation: CAK86280.1
PIRiS65570
RefSeqiXP_001453677.1, XM_001453640.1

Genome annotation databases

EnsemblProtistsiCAK86280; CAK86280; GSPATT00019973001
GeneIDi5039462
KEGGiptm:GSPATT00019973001

Similar proteinsi

Entry informationi

Entry nameiDRTS_PARTE
AccessioniPrimary (citable) accession number: Q27828
Secondary accession number(s): A0DTB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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