Reviewed, UniProtKB/Swiss-Prot Q27828 (DRTS_PARTE)

Names and origin

Protein names

Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
      Short name=DHFR-TS
Including the following 2 domains:
    1- Recommended name:
            Dihydrofolate reductase
              EC=1.5.1.3
    2- Recommended name:
            Thymidylate synthase
              EC=2.1.1.45

Gene names

ORF Names:

GSPATT00019973001

Organism

Paramecium tetraurelia

Taxonomic identifier

5888 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Peniculida › Parameciidae › Paramecium

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH. 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	In the N-terminal section; belongs to the dihydrofolate reductase family. In the C-terminal section; belongs to the thymidylate synthase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis One-carbon metabolism
Ligand	NADP
Molecular function	Methyltransferase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro glycine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro thymidylate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Bifunctional dihydrofolate reductase-thymidylate synthase		PRO_0000186346
Regions
	Domain	6 – 165	160	DHFR
	Region	180 – 462	283	Thymidylate synthase
Sites
	Active site	345	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27828-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C8C9B3A12BBD5C72	FASTA	462	53,201
	10 20 30 40 50 60 MTTNKTFSMV LAMTLNGGIG YQNRLPWKLK EDLQRFKKIT TGGIVIMGRK TFESMNSKPL 70 80 90 100 110 120 PNRVNVVISK NMKSSNEVQV FPRIEDALQF YNTSHQKLYL IGGKRIFEEG LATDKCSDVH 130 140 150 160 170 180 LTRIGVETKC DVYLNKNIFS TFQVNKTSQT KSENGINYDY QHLINKNSHE QSYIDEEHQE 190 200 210 220 230 240 NQYLDMITKI MKEGVSKDDR TGVGTMSIFG QTMRFNLAQS FPLLTTKKVF FRGVVEELLW 250 260 270 280 290 300 FLRGNTNGKL LLDKGVKIWE GNGTREYLDT IGLQHRQEHD LGPVYGFQWR HFGAKYKDCQ 310 320 330 340 350 360 TDYSNQGVDQ VKEIIQLLKN NPDSRRIILS AWNPIDLKQM ALPPCHVMSQ FFVANGKLSC 370 380 390 400 410 420 MMYQRSCDFG LGIPFNIASY ALLTYMLAKE CNLNLGEFVH VLGDTHIYSN HVEALKKQIE 430 440 450 460 RVPYPFPLLK IKGNKSLFDY TYEDFELVGY NAHDKIEMKM AV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and molecular analysis of the bifunctional dihydrofolate reductase-thymidylate synthase gene in the ciliated protozoan Paramecium tetraurelia." Schlichtherle I.M., van Houten J.L., Roos D.S. Mol. Gen. Genet. 250:665-673(1996) [PubMed: 8628226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Stock 51.
[2]	"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia." Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P. Nature 444:171-178(2006) [PubMed: 17086204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Stock d4-2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U03885 Genomic DNA. Translation: AAC47025.1. CT868563 Genomic DNA. Translation: CAK86280.1.
PIR	S65570.
RefSeq	XP_001453677.1.
3D structure databases
HSSP	HSSP built from PDB template 1HW4 based on UniProtKB P04818.
ModBase	Search...
Genome annotation databases
GeneID	5039462.
KEGG	ptm:GSPATT00019973001.
Family and domain databases
InterPro	IPR012262. DHFR-TS. IPR001796. DHFR_reg. IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PIRSF	PIRSF000389. DHFR-TS. 1 hit.
PRINTS	PR00070. DHFR. PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRTS_PARTE

Accession

Primary (citable) accession number: Q27828
Secondary accession number(s): A0DTB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)