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Q27828

- DRTS_PARTE

UniProt

Q27828 - DRTS_PARTE

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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

GSPATT00019973001

Organism
Paramecium tetraurelia
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Substrate; via carbonyl oxygenBy similarity
Binding sitei12 – 121NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei32 – 321SubstrateBy similarity
Binding sitei101 – 1011Substrate; via carbonyl oxygenBy similarity
Binding sitei122 – 1221SubstrateBy similarity
Binding sitei200 – 2001dUMPBy similarity
Active sitei345 – 3451By similarity
Binding sitei346 – 3461dUMPBy similarity
Binding sitei376 – 3761dUMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 247NADPBy similarity
Nucleotide bindingi49 – 513NADPBy similarity
Nucleotide bindingi68 – 714NADPBy similarity
Nucleotide bindingi102 – 1098NADPBy similarity
Nucleotide bindingi364 – 3685dUMPBy similarity
Nucleotide bindingi406 – 4083dUMPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
ORF Names:GSPATT00019973001
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
ProteomesiUP000000600: Partially assembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186346Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ27828.
SMRiQ27828. Positions 138-462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 165160DHFRAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 462283Thymidylate synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

InParanoidiQ27828.
KOiK13998.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27828-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTNKTFSMV LAMTLNGGIG YQNRLPWKLK EDLQRFKKIT TGGIVIMGRK
60 70 80 90 100
TFESMNSKPL PNRVNVVISK NMKSSNEVQV FPRIEDALQF YNTSHQKLYL
110 120 130 140 150
IGGKRIFEEG LATDKCSDVH LTRIGVETKC DVYLNKNIFS TFQVNKTSQT
160 170 180 190 200
KSENGINYDY QHLINKNSHE QSYIDEEHQE NQYLDMITKI MKEGVSKDDR
210 220 230 240 250
TGVGTMSIFG QTMRFNLAQS FPLLTTKKVF FRGVVEELLW FLRGNTNGKL
260 270 280 290 300
LLDKGVKIWE GNGTREYLDT IGLQHRQEHD LGPVYGFQWR HFGAKYKDCQ
310 320 330 340 350
TDYSNQGVDQ VKEIIQLLKN NPDSRRIILS AWNPIDLKQM ALPPCHVMSQ
360 370 380 390 400
FFVANGKLSC MMYQRSCDFG LGIPFNIASY ALLTYMLAKE CNLNLGEFVH
410 420 430 440 450
VLGDTHIYSN HVEALKKQIE RVPYPFPLLK IKGNKSLFDY TYEDFELVGY
460
NAHDKIEMKM AV
Length:462
Mass (Da):53,201
Last modified:November 1, 1996 - v1
Checksum:iC8C9B3A12BBD5C72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03885 Genomic DNA. Translation: AAC47025.1.
CT868563 Genomic DNA. Translation: CAK86280.1.
PIRiS65570.
RefSeqiXP_001453677.1. XM_001453640.1.

Genome annotation databases

EnsemblProtistsiCAK86280; CAK86280; GSPATT00019973001.
GeneIDi5039462.
KEGGiptm:GSPATT00019973001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03885 Genomic DNA. Translation: AAC47025.1 .
CT868563 Genomic DNA. Translation: CAK86280.1 .
PIRi S65570.
RefSeqi XP_001453677.1. XM_001453640.1.

3D structure databases

ProteinModelPortali Q27828.
SMRi Q27828. Positions 138-462.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi CAK86280 ; CAK86280 ; GSPATT00019973001 .
GeneIDi 5039462.
KEGGi ptm:GSPATT00019973001.

Phylogenomic databases

InParanoidi Q27828.
KOi K13998.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and molecular analysis of the bifunctional dihydrofolate reductase-thymidylate synthase gene in the ciliated protozoan Paramecium tetraurelia."
    Schlichtherle I.M., van Houten J.L., Roos D.S.
    Mol. Gen. Genet. 250:665-673(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Stock 51.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Stock d4-2.

Entry informationi

Entry nameiDRTS_PARTE
AccessioniPrimary (citable) accession number: Q27828
Secondary accession number(s): A0DTB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3