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Q27828

- DRTS_PARTE

UniProt

Q27828 - DRTS_PARTE

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

GSPATT00019973001

Organism
Paramecium tetraurelia
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Substrate; via carbonyl oxygenBy similarity
    Binding sitei12 – 121NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei32 – 321SubstrateBy similarity
    Binding sitei101 – 1011Substrate; via carbonyl oxygenBy similarity
    Binding sitei122 – 1221SubstrateBy similarity
    Binding sitei200 – 2001dUMPBy similarity
    Active sitei345 – 3451By similarity
    Binding sitei346 – 3461dUMPBy similarity
    Binding sitei376 – 3761dUMPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 247NADPBy similarity
    Nucleotide bindingi49 – 513NADPBy similarity
    Nucleotide bindingi68 – 714NADPBy similarity
    Nucleotide bindingi102 – 1098NADPBy similarity
    Nucleotide bindingi364 – 3685dUMPBy similarity
    Nucleotide bindingi406 – 4083dUMPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. glycine biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
    Short name:
    DHFR-TS
    Including the following 2 domains:
    Dihydrofolate reductase (EC:1.5.1.3)
    Thymidylate synthase (EC:2.1.1.45)
    Gene namesi
    ORF Names:GSPATT00019973001
    OrganismiParamecium tetraurelia
    Taxonomic identifieri5888 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
    ProteomesiUP000000600: Partially assembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186346Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ27828.
    SMRiQ27828. Positions 138-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 165160DHFRAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 462283Thymidylate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
    In the C-terminal section; belongs to the thymidylate synthase family.Curated

    Phylogenomic databases

    KOiK13998.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000389. DHFR-TS. 1 hit.
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27828-1 [UniParc]FASTAAdd to Basket

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    MTTNKTFSMV LAMTLNGGIG YQNRLPWKLK EDLQRFKKIT TGGIVIMGRK    50
    TFESMNSKPL PNRVNVVISK NMKSSNEVQV FPRIEDALQF YNTSHQKLYL 100
    IGGKRIFEEG LATDKCSDVH LTRIGVETKC DVYLNKNIFS TFQVNKTSQT 150
    KSENGINYDY QHLINKNSHE QSYIDEEHQE NQYLDMITKI MKEGVSKDDR 200
    TGVGTMSIFG QTMRFNLAQS FPLLTTKKVF FRGVVEELLW FLRGNTNGKL 250
    LLDKGVKIWE GNGTREYLDT IGLQHRQEHD LGPVYGFQWR HFGAKYKDCQ 300
    TDYSNQGVDQ VKEIIQLLKN NPDSRRIILS AWNPIDLKQM ALPPCHVMSQ 350
    FFVANGKLSC MMYQRSCDFG LGIPFNIASY ALLTYMLAKE CNLNLGEFVH 400
    VLGDTHIYSN HVEALKKQIE RVPYPFPLLK IKGNKSLFDY TYEDFELVGY 450
    NAHDKIEMKM AV 462
    Length:462
    Mass (Da):53,201
    Last modified:November 1, 1996 - v1
    Checksum:iC8C9B3A12BBD5C72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03885 Genomic DNA. Translation: AAC47025.1.
    CT868563 Genomic DNA. Translation: CAK86280.1.
    PIRiS65570.
    RefSeqiXP_001453677.1. XM_001453640.1.

    Genome annotation databases

    EnsemblProtistsiCAK86280; CAK86280; GSPATT00019973001.
    GeneIDi5039462.
    KEGGiptm:GSPATT00019973001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03885 Genomic DNA. Translation: AAC47025.1 .
    CT868563 Genomic DNA. Translation: CAK86280.1 .
    PIRi S65570.
    RefSeqi XP_001453677.1. XM_001453640.1.

    3D structure databases

    ProteinModelPortali Q27828.
    SMRi Q27828. Positions 138-462.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi CAK86280 ; CAK86280 ; GSPATT00019973001 .
    GeneIDi 5039462.
    KEGGi ptm:GSPATT00019973001.

    Phylogenomic databases

    KOi K13998.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000389. DHFR-TS. 1 hit.
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and molecular analysis of the bifunctional dihydrofolate reductase-thymidylate synthase gene in the ciliated protozoan Paramecium tetraurelia."
      Schlichtherle I.M., van Houten J.L., Roos D.S.
      Mol. Gen. Genet. 250:665-673(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Stock 51.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Stock d4-2.

    Entry informationi

    Entry nameiDRTS_PARTE
    AccessioniPrimary (citable) accession number: Q27828
    Secondary accession number(s): A0DTB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3