Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q27828 (DRTS_PARTE)

Last modified November 25, 2008. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
      Short name=DHFR-TS
Including the following 2 domains:
    1- Recommended name:
            Dihydrofolate reductase
              EC=1.5.1.3
    2- Recommended name:
            Thymidylate synthase
              EC=2.1.1.45
Gene names
ORF Names: GSPATT00019973001
OrganismParamecium tetraurelia
Taxonomic identifier5888 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.

Miscellaneous

The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Sequence similarities

In the N-terminal section; belongs to the dihydrofolate reductase family.

In the C-terminal section; belongs to the thymidylate synthase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Bifunctional dihydrofolate reductase-thymidylate synthase
PRO_0000186346

Regions

Domain6 – 165160DHFR
Region180 – 462283Thymidylate synthase

Sites

Active site3451 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27828-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C8C9B3A12BBD5C72

FASTA46253,201
        10         20         30         40         50         60 
MTTNKTFSMV LAMTLNGGIG YQNRLPWKLK EDLQRFKKIT TGGIVIMGRK TFESMNSKPL 

        70         80         90        100        110        120 
PNRVNVVISK NMKSSNEVQV FPRIEDALQF YNTSHQKLYL IGGKRIFEEG LATDKCSDVH 

       130        140        150        160        170        180 
LTRIGVETKC DVYLNKNIFS TFQVNKTSQT KSENGINYDY QHLINKNSHE QSYIDEEHQE 

       190        200        210        220        230        240 
NQYLDMITKI MKEGVSKDDR TGVGTMSIFG QTMRFNLAQS FPLLTTKKVF FRGVVEELLW 

       250        260        270        280        290        300 
FLRGNTNGKL LLDKGVKIWE GNGTREYLDT IGLQHRQEHD LGPVYGFQWR HFGAKYKDCQ 

       310        320        330        340        350        360 
TDYSNQGVDQ VKEIIQLLKN NPDSRRIILS AWNPIDLKQM ALPPCHVMSQ FFVANGKLSC 

       370        380        390        400        410        420 
MMYQRSCDFG LGIPFNIASY ALLTYMLAKE CNLNLGEFVH VLGDTHIYSN HVEALKKQIE 

       430        440        450        460 
RVPYPFPLLK IKGNKSLFDY TYEDFELVGY NAHDKIEMKM AV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and molecular analysis of the bifunctional dihydrofolate reductase-thymidylate synthase gene in the ciliated protozoan Paramecium tetraurelia."
Schlichtherle I.M., van Houten J.L., Roos D.S.
Mol. Gen. Genet. 250:665-673(1996) [PubMed: 8628226] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Stock 51.
[2]"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia."
Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. expand/collapse author list , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P.
Nature 444:171-178(2006) [PubMed: 17086204] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Stock d4-2.

Cross-references

Sequence databases

U03885 Genomic DNA. Translation: AAC47025.1.
CT868563 Genomic DNA. Translation: CAK86280.1.
PIRS65570.
RefSeqXP_001453677.1.

3D structure databases

HSSPHSSP built from PDB template 1HW4 based on UniProtKB P04818.
ModBaseSearch...

Genome annotation databases

GeneID5039462.
KEGGptm:GSPATT00019973001.

Family and domain databases

InterProIPR012262. DHFR-TS.
IPR001796. DHFR_reg.
IPR000398. Thymidylat_synth_C.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFPIRSF000389. DHFR-TS. 1 hit.
PRINTSPR00070. DHFR.
PR00108. THYMDSNTHASE.
ProDomPD001180. Thymidylat_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDRTS_PARTE
AccessionPrimary (citable) accession number: Q27828
Secondary accession number(s): A0DTB3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents