Q27796 (Q27796_TRYCR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Hypoxanthine-guanine phosphoribosyltransferase EMBL AAB46391.1
EC=2.4.2.8 EMBL AAB46391.1

Gene names

Name:

HGPRTase EMBL AAB46391.1

Organism

Trypanosoma cruzi EMBL AAB46391.1

Taxonomic identifier

5693 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Schizotrypanum

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosyltransferase EMBL AAB46391.1 Transferase
Technical term	3D-structure PDB 1TC1 PDB 1I13 PDB 1I14 PDB 1I0I PDB 1I0L PDB 1P17 PDB 1P18 PDB 1P19 PDB 1TC2
Gene Ontology (GO)
Biological process	purine ribonucleoside salvage Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	guanine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC hypoxanthine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q27796 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B12CCE38FF149800	FASTA	221	25,529
	10 20 30 40 50 60 MPREYEFAEK ILFTEEEIRT RIMEVAKRIA DDYKGKGLRP YVNPLVLISV LKGSFMFTAD 70 80 90 100 110 120 LCRALSDFNV PVRMEFICVS SYGEGVTSSG QVRMLLDTRH SIEGHHVLIV EDIVDTALTL 130 140 150 160 170 180 NYLYHMYFTR RPASLKTVVL LDKREGRRVP FSADYVVANI PNAFVIGYGL DYDDTYRELR 190 200 210 220 DIVVLRPEVY AEREAARQKK QRAIGSADTD RDAKREFHSK Y

References

[1]	"Molecular characterization and overexpression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma cruzi." Allen T.E., Ullman B. Mol. Biochem. Parasitol. 65:233-245(1994) [PubMed: 7969265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: CL EMBL AAB46391.1.
[2]	"A 1.4 A crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi." Focia P.J., Craig S.P., Nieves-Alicea R., Fletterick R.J., Eakin A.E. Biochemistry 37:15066-15075(1998) [PubMed: 9790669] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.41 ANGSTROMS) OF 2-221.
[3]	"The role for an invariant aspartic acid in hypoxanthine phosphoribosyltransferases is examined using saturation mutagenesis, functional analysis, and X-ray crystallography." Canyuk B., Focia P.J., Eakin A.E. Biochemistry 40:2754-2765(2001) [PubMed: 11258886] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS).
[4]	"Interactions at the dimer interface influence the relative efficiencies for purine nucleotide synthesis and pyrophosphorolysis in a phosphoribosyltransferase." Canyuk B., Medrano F.J., Wenck M.A., Focia P.J., Eakin A.E., Craig S.P. J. Mol. Biol. 335:905-921(2004) [PubMed: 14698288] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[5]	"Approaching the transition state in the crystal structure of a phosphoribosyltransferase." Focia P.J., Craig S.P., Eakin A.E. Biochemistry 37:17120-17127(1998) [PubMed: 9860824] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L07486 Genomic DNA. Translation: AAB46391.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I0I	X-ray	2.06	A/B	1-221	[»]
1I0L	X-ray	1.72	A/B	1-221	[»]
1I13	X-ray	1.84	A/B	1-221	[»]
1I14	X-ray	1.92	A/B	1-221	[»]
1P17	X-ray	2.70	A/B/C/D	1-221	[»]
1P18	X-ray	2.00	A/B	1-221	[»]
1P19	X-ray	2.30	A/B/C/D	1-221	[»]
1TC1	X-ray	1.41	A/B	2-221	[»]
1TC2	X-ray	1.81	A/B	1-221	[»]

ProteinModelPortal

Q27796.

SMR

Q27796. Positions 2-211.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Genome annotation databases

KEGG

tcr:506457.30.

Organism-specific databases

EuPathDB

EupathDB:Tc00.1047053506457.30.

Phylogenomic databases

ProtClustDB

CLSZ2441710.

Family and domain databases

InterPro

IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase.
[Graphical view]

KO

K00760.

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01203. HGPRTase. 1 hit.

ProtoNet

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Entry information

Entry name

Q27796_TRYCR

Accession

Primary (citable) accession number: Q27796

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)