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Reviewed, UniProtKB/Swiss-Prot Q27793 (DRTS_TRYCR)

Last modified November 25, 2008. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
      Short name=DHFR-TS
Including the following 2 domains:
    1- Recommended name:
            Dihydrofolate reductase
              EC=1.5.1.3
    2- Recommended name:
            Thymidylate synthase
              EC=2.1.1.45
OrganismTrypanosoma cruzi
Taxonomic identifier5693 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.

Miscellaneous

The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Sequence similarities

In the N-terminal section; belongs to the dihydrofolate reductase family.

In the C-terminal section; belongs to the thymidylate synthase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 521521Bifunctional dihydrofolate reductase-thymidylate synthase
PRO_0000186354

Regions

Domain22 – 232211DHFR
Region237 – 521285Thymidylate synthase

Sites

Active site4031 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27793-1 [UniParc].

Last modified May 1, 1997. Version 2.
Checksum: DCB9F8782855810C

FASTA52158,853
        10         20         30         40         50         60 
MSLFKIRMPE TVAEGTRLAL RAFSLVVAVD EHGGIGDGRS IPWNVPEDMK FFRDLTTKLR 

        70         80         90        100        110        120 
GKNVKPSPAK RNAVVMGRKT WDSIPPKFRP LPGRLNVVLS STLTTQHLLD GLPDEEKRNL 

       130        140        150        160        170        180 
HADSIVAVNG GLEQALRLLA SPNYTPSIET VYCIGGGSVY AEALRPPCVH LLQAIYRTTI 

       190        200        210        220        230        240 
RASESSCSVF FRVPESGTEA AAGIEWQRET ISEELTSANG NETKYYFEKL IPRNREEEQY 

       250        260        270        280        290        300 
LSLVDRIIRE GNVKHDRTGV GTLSIFGAQM RFSLRNNRLP LLTTKRVFWR GVCEELLWFL 

       310        320        330        340        350        360 
RGETYAKKLS DKGVHIWDDN GSRAFLDSRG LTEYEEMDLG PVYGFQWRHF GAAYTHHDAN 

       370        380        390        400        410        420 
YDGQGVDQIK AIVETLKTNP DDRRMLFTAW NPSALPRMAL PPCHLLAQFY VSNGELSCML 

       430        440        450        460        470        480 
YQRSCDMGLG VPFNIASYAL LTILIAKATG LRPGELVHTL GDAHVYSNHV EPCNEQLKRV 

       490        500        510        520 
PRAFPYLVFR REREFLEDYE EGDMEVIDYA PYPPISMKMA V 

« Hide

References

[1]"Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi."
Reche P., Arrebola R., Olmo A., Santi D.V., Gonzalez-Pacanowska D., Ruiz-Perez L.M.
Mol. Biochem. Parasitol. 65:247-258(1994) [PubMed: 7969266] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Y.
[2]"Expression and characterization of the Trypanosoma cruzi dihydrofolate reductase domain."
Reche P., Arrebola R., Santi D.V., Gonzalez-Pacanowska D., Ruiz-Perez L.M.
Mol. Biochem. Parasitol. 76:175-185(1996) [PubMed: 8920005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Y.

Cross-references

Sequence databases

L22484 Genomic DNA. Translation: AAB49898.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2H2QX-ray2.40A/B1-521[»]
ModBaseSearch...

Family and domain databases

InterProIPR012262. DHFR-TS.
IPR001796. DHFR_reg.
IPR000398. Thymidylat_synth_C.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFPIRSF000389. DHFR-TS. 1 hit.
PRINTSPR00070. DHFR.
PR00108. THYMDSNTHASE.
ProDomPD001180. Thymidylat_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDRTS_TRYCR
AccessionPrimary (citable) accession number: Q27793
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 25, 2008
This is version 43 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents