ID   KPYK_TRYBO              Reviewed;         498 AA.
AC   Q27788; Q27789;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Pyruvate kinase;
DE            Short=PK;
DE            EC=2.7.1.40;
GN   Name=PYK;
OS   Trypanoplasma borreli.
OC   Eukaryota; Euglenozoa; Kinetoplastida; Bodonidae; Trypanoplasma.
OX   NCBI_TaxID=5710;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=94271225; PubMed=8003008; DOI=10.1006/bbrc.1994.1761;
RA   Ernest I., Opperdoes F.R., Michels P.A.M.;
RT   "Cloning and sequence analysis of the gene encoding pyruvate kinase in
RT   Trypanoplasma borelli.";
RL   Biochem. Biophys. Res. Commun. 201:727-732(1994).
CC   -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate.
CC   -!- COFACTOR: Magnesium.
CC   -!- COFACTOR: Potassium.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 5/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X77255; CAA54472.1; -; Genomic_DNA.
DR   EMBL; X77255; CAA54473.1; -; Genomic_DNA.
DR   PIR; JC2456; JC2456.
DR   HSSP; P14178; 1E0T.
DR   BRENDA; 2.7.1.40; 270090.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR   GO; GO:0004743; F:pyruvate kinase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR001697; Pyr_Knase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase_cat.
DR   InterPro; IPR015794; Pyrv_Knase_a/b.
DR   InterPro; IPR018209; Pyrv_Knase_AS.
DR   InterPro; IPR015793; Pyrv_Knase_brl.
DR   Gene3D; G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   Gene3D; G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1.
DR   PANTHER; PTHR11817; Pyruvate_kinase; 1.
DR   Pfam; PF00224; PK; 1.
DR   Pfam; PF02887; PK_C; 1.
DR   PRINTS; PR01050; PYRUVTKNASE.
DR   ProDom; PD001009; Pyruvate_kinase; 2.
DR   TIGRFAMs; TIGR01064; pyruv_kin; 1.
DR   PROSITE; PS00110; PYRUVATE_KINASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Glycolysis; Kinase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Pyruvate; Transferase.
FT   CHAIN         1    498       Pyruvate kinase.
FT                                /FTId=PRO_0000112105.
FT   ACT_SITE    238    238       By similarity.
FT   METAL       240    240       Magnesium (By similarity).
FT   METAL       261    261       Magnesium (By similarity).
FT   METAL       262    262       Magnesium (By similarity).
SQ   SEQUENCE   498 AA;  54634 MW;  7C5882430B93DE21 CRC64;
     MMRKSQLQFN TELRVHHPPA LFRSNKIICT IGPSSQSVEV LKDLMKAGLN VARMNFSHGT
     YEYHQKTIDN VRKAASELGI HVGIALDTKG PEIRTGLFPA GDVVIEAHKT VILTTDETFK
     EKGTAEKFYV DYMNITKVVP VGGHIFVDDG LLDLIVVKIS GKDIECVAQN THTISNRKGI
     NLPNADVDLP AVSEKDLMDL QFGAKNRVDF VFASFIRNAD QVNEVRQAFG GKIAVIAKIE
     NYQGIDNIDA IIDAADGIMV ARGDLGVEIP AEKVVIAQKM IMSKCNKVGK TVICATQMLD
     SMTHGPRPTR AEVSDVAKSV LDGADCVMLS GETAKGKYPV ETVVYMSRIC CETQVTMWNM
     AAFEAIKNLQ SFPLIPEEAI CSSAVNSIFE LHAKAILVLT NTGRSAHMVS KYRPPVPIIC
     ASQELDVCRL LSITRGTIPV YYDTEKLGPD YDREKRVGLA IDVGKQMGVF KEGDVVVAVH
     ADHHTKGFAN QIRAIYIK
//