Reviewed, UniProtKB/Swiss-Prot Q27788 (KPYK_TRYBO)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:

PYK

Organism

Trypanoplasma borreli

Taxonomic identifier

5710 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Bodonidae › Trypanoplasma

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Pyruvate kinase		PRO_0000112105
Sites
	Active site	238	1	By similarity
	Metal binding	240	1	Magnesium By similarity
	Metal binding	261	1	Magnesium By similarity
	Metal binding	262	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27788-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7C5882430B93DE21	FASTA	498	54,634
	10 20 30 40 50 60 MMRKSQLQFN TELRVHHPPA LFRSNKIICT IGPSSQSVEV LKDLMKAGLN VARMNFSHGT 70 80 90 100 110 120 YEYHQKTIDN VRKAASELGI HVGIALDTKG PEIRTGLFPA GDVVIEAHKT VILTTDETFK 130 140 150 160 170 180 EKGTAEKFYV DYMNITKVVP VGGHIFVDDG LLDLIVVKIS GKDIECVAQN THTISNRKGI 190 200 210 220 230 240 NLPNADVDLP AVSEKDLMDL QFGAKNRVDF VFASFIRNAD QVNEVRQAFG GKIAVIAKIE 250 260 270 280 290 300 NYQGIDNIDA IIDAADGIMV ARGDLGVEIP AEKVVIAQKM IMSKCNKVGK TVICATQMLD 310 320 330 340 350 360 SMTHGPRPTR AEVSDVAKSV LDGADCVMLS GETAKGKYPV ETVVYMSRIC CETQVTMWNM 370 380 390 400 410 420 AAFEAIKNLQ SFPLIPEEAI CSSAVNSIFE LHAKAILVLT NTGRSAHMVS KYRPPVPIIC 430 440 450 460 470 480 ASQELDVCRL LSITRGTIPV YYDTEKLGPD YDREKRVGLA IDVGKQMGVF KEGDVVVAVH 490 ADHHTKGFAN QIRAIYIK

References

[1]	"Cloning and sequence analysis of the gene encoding pyruvate kinase in Trypanoplasma borelli." Ernest I., Opperdoes F.R., Michels P.A.M. Biochem. Biophys. Res. Commun. 201:727-732(1994) [PubMed: 8003008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77255 Genomic DNA. Translation: CAA54472.1. X77255 Genomic DNA. Translation: CAA54473.1.
PIR	JC2456.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 270090.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_TRYBO

Accession

Primary (citable) accession number: Q27788
Secondary accession number(s): Q27789

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)