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Q27788 (KPYK_TRYBO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:PYK
OrganismTrypanoplasma borreli
Taxonomic identifier5710 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaBodonidaeTrypanoplasma

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Pyruvate kinase
PRO_0000112105

Sites

Metal binding551Potassium By similarity
Metal binding571Potassium By similarity
Metal binding871Potassium By similarity
Metal binding881Potassium; via carbonyl oxygen By similarity
Metal binding2401Magnesium By similarity
Metal binding2641Magnesium By similarity
Binding site531Substrate By similarity
Binding site2631Substrate; via amide nitrogen By similarity
Binding site2641Substrate; via amide nitrogen By similarity
Binding site2961Substrate By similarity
Site2381Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27788 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7C5882430B93DE21

FASTA49854,634
        10         20         30         40         50         60 
MMRKSQLQFN TELRVHHPPA LFRSNKIICT IGPSSQSVEV LKDLMKAGLN VARMNFSHGT 

        70         80         90        100        110        120 
YEYHQKTIDN VRKAASELGI HVGIALDTKG PEIRTGLFPA GDVVIEAHKT VILTTDETFK 

       130        140        150        160        170        180 
EKGTAEKFYV DYMNITKVVP VGGHIFVDDG LLDLIVVKIS GKDIECVAQN THTISNRKGI 

       190        200        210        220        230        240 
NLPNADVDLP AVSEKDLMDL QFGAKNRVDF VFASFIRNAD QVNEVRQAFG GKIAVIAKIE 

       250        260        270        280        290        300 
NYQGIDNIDA IIDAADGIMV ARGDLGVEIP AEKVVIAQKM IMSKCNKVGK TVICATQMLD 

       310        320        330        340        350        360 
SMTHGPRPTR AEVSDVAKSV LDGADCVMLS GETAKGKYPV ETVVYMSRIC CETQVTMWNM 

       370        380        390        400        410        420 
AAFEAIKNLQ SFPLIPEEAI CSSAVNSIFE LHAKAILVLT NTGRSAHMVS KYRPPVPIIC 

       430        440        450        460        470        480 
ASQELDVCRL LSITRGTIPV YYDTEKLGPD YDREKRVGLA IDVGKQMGVF KEGDVVVAVH 

       490 
ADHHTKGFAN QIRAIYIK 

« Hide

References

[1]"Cloning and sequence analysis of the gene encoding pyruvate kinase in Trypanoplasma borelli."
Ernest I., Opperdoes F.R., Michels P.A.M.
Biochem. Biophys. Res. Commun. 201:727-732(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77255 Genomic DNA. Translation: CAA54472.1.
X77255 Genomic DNA. Translation: CAA54473.1.
PIRJC2456.

3D structure databases

ProteinModelPortalQ27788.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_TRYBO
AccessionPrimary (citable) accession number: Q27788
Secondary accession number(s): Q27789
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways