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Protein

Pyruvate kinase

Gene

PYK

Organism
Trypanoplasma borreli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway:iglycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap), Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (PYK)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Metal bindingi55 – 551PotassiumBy similarity
Metal bindingi57 – 571PotassiumBy similarity
Metal bindingi87 – 871PotassiumBy similarity
Metal bindingi88 – 881Potassium; via carbonyl oxygenBy similarity
Sitei238 – 2381Transition state stabilizerBy similarity
Metal bindingi240 – 2401MagnesiumBy similarity
Binding sitei263 – 2631Substrate; via amide nitrogenBy similarity
Metal bindingi264 – 2641MagnesiumBy similarity
Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
Binding sitei296 – 2961SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK
OrganismiTrypanoplasma borreli
Taxonomic identifieri5710 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaBodonidaeTrypanoplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Pyruvate kinasePRO_0000112105Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ27788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRKSQLQFN TELRVHHPPA LFRSNKIICT IGPSSQSVEV LKDLMKAGLN
60 70 80 90 100
VARMNFSHGT YEYHQKTIDN VRKAASELGI HVGIALDTKG PEIRTGLFPA
110 120 130 140 150
GDVVIEAHKT VILTTDETFK EKGTAEKFYV DYMNITKVVP VGGHIFVDDG
160 170 180 190 200
LLDLIVVKIS GKDIECVAQN THTISNRKGI NLPNADVDLP AVSEKDLMDL
210 220 230 240 250
QFGAKNRVDF VFASFIRNAD QVNEVRQAFG GKIAVIAKIE NYQGIDNIDA
260 270 280 290 300
IIDAADGIMV ARGDLGVEIP AEKVVIAQKM IMSKCNKVGK TVICATQMLD
310 320 330 340 350
SMTHGPRPTR AEVSDVAKSV LDGADCVMLS GETAKGKYPV ETVVYMSRIC
360 370 380 390 400
CETQVTMWNM AAFEAIKNLQ SFPLIPEEAI CSSAVNSIFE LHAKAILVLT
410 420 430 440 450
NTGRSAHMVS KYRPPVPIIC ASQELDVCRL LSITRGTIPV YYDTEKLGPD
460 470 480 490
YDREKRVGLA IDVGKQMGVF KEGDVVVAVH ADHHTKGFAN QIRAIYIK
Length:498
Mass (Da):54,634
Last modified:November 1, 1996 - v1
Checksum:i7C5882430B93DE21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77255 Genomic DNA. Translation: CAA54472.1.
X77255 Genomic DNA. Translation: CAA54473.1.
PIRiJC2456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77255 Genomic DNA. Translation: CAA54472.1.
X77255 Genomic DNA. Translation: CAA54473.1.
PIRiJC2456.

3D structure databases

ProteinModelPortaliQ27788.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of the gene encoding pyruvate kinase in Trypanoplasma borelli."
    Ernest I., Opperdoes F.R., Michels P.A.M.
    Biochem. Biophys. Res. Commun. 201:727-732(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKPYK_TRYBO
AccessioniPrimary (citable) accession number: Q27788
Secondary accession number(s): Q27789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.