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Q27783

- DRTS_TRYBB

UniProt

Q27783 - DRTS_TRYBB

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.By similarity

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321Substrate; via carbonyl oxygenBy similarity
    Binding sitei34 – 341NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei54 – 541SubstrateBy similarity
    Binding sitei160 – 1601Substrate; via carbonyl oxygenBy similarity
    Binding sitei166 – 1661SubstrateBy similarity
    Binding sitei184 – 1841SubstrateBy similarity
    Binding sitei263 – 2631dUMPBy similarity
    Active sitei409 – 4091By similarity
    Binding sitei410 – 4101dUMPBy similarity
    Binding sitei440 – 4401dUMPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 467NADPBy similarity
    Nucleotide bindingi84 – 863NADPBy similarity
    Nucleotide bindingi105 – 1084NADPBy similarity
    Nucleotide bindingi161 – 1688NADPBy similarity
    Nucleotide bindingi428 – 4325dUMPBy similarity
    Nucleotide bindingi470 – 4723dUMPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway
    3. glycine biosynthetic process Source: InterPro
    4. one-carbon metabolic process Source: UniProtKB-KW
    5. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.
    UPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
    Short name:
    DHFR-TS
    Including the following 2 domains:
    Dihydrofolate reductase (EC:1.5.1.3)
    Thymidylate synthase (EC:2.1.1.45)
    OrganismiTrypanosoma brucei brucei
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 527527Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186353Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    Secondary structure

    1
    527
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi29 – 368
    Beta strandi40 – 467
    Helixi52 – 6312
    Beta strandi66 – 694
    Beta strandi76 – 838
    Helixi84 – 896
    Helixi92 – 943
    Beta strandi100 – 1056
    Helixi111 – 1155
    Helixi121 – 1277
    Helixi128 – 1303
    Beta strandi131 – 1366
    Helixi138 – 1447
    Helixi148 – 1536
    Beta strandi154 – 1596
    Helixi163 – 1697
    Helixi174 – 1774
    Beta strandi178 – 18811
    Beta strandi212 – 2176
    Beta strandi230 – 2389

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3QFXX-ray2.20A/B1-241[»]
    3RG9X-ray2.00A/B1-240[»]
    ProteinModelPortaliQ27783.
    SMRiQ27783. Positions 11-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ27783.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 238211DHFRAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni243 – 527285Thymidylate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
    In the C-terminal section; belongs to the thymidylate synthase family.Curated

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000389. DHFR-TS. 1 hit.
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27783-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSLTRILRK KIPVHELAGK ISRPPLRPFS VVVASDEKGG IGDGGTIPWE    50
    IPEDMQYFRR VTTNLRGKNV KPSPSKRNAV VMGRKTWDSL PPKFRPLSNR 100
    LNVVLSRSAT KEQLLAGIPD PIKRAEAAND VVAVNGGLED ALRMLVSKEH 150
    TSSIETVFCI GGGTIYKQAL CAPCVNVLQA IHRTVVRPAS NSCSVFFDIP 200
    AAGTKTPEGL ELVRESITDE RVSTGAGGKK YQFEKLVPRN SEEEQYLNLV 250
    GRIIDEGCTK CDRTGVGTRS LFGAQMRFSL RNNRLPLLTT KRVFWRGVCE 300
    ELLWFLRGET NAKLLSDKGI HIWDGNGSRA FLDSRGLTDY DEMDLGPVYG 350
    FQWRHFGADY ISCKVDSEGK GVDQIANIVK SLIENPDDRR MICTAWNPAA 400
    LPRMALPPCH MMAQFYVSNG ELSCMLYQRS CDMGLGVPFN IASYALLTFL 450
    MAKASGLRPG ELVHTLGDAH VYSNHVEPCR KQLKRVPRPF PFIVFKQDKE 500
    FLEDFQESDI EVIDYSPYPV ISMEMAV 527
    Length:527
    Mass (Da):58,805
    Last modified:November 1, 1996 - v1
    Checksum:i39982FC553BF7601
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20781 Genomic DNA. Translation: AAA91362.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20781 Genomic DNA. Translation: AAA91362.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3QFX X-ray 2.20 A/B 1-241 [» ]
    3RG9 X-ray 2.00 A/B 1-240 [» ]
    ProteinModelPortali Q27783.
    SMRi Q27783. Positions 11-521.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    UPA00575 .

    Miscellaneous databases

    EvolutionaryTracei Q27783.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000389. DHFR-TS. 1 hit.
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme."
      Gamarro F., Yu P.L., Zhao J., Edman U., Greene P.J., Santi D.
      Mol. Biochem. Parasitol. 72:11-22(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 427.

    Entry informationi

    Entry nameiDRTS_TRYBB
    AccessioniPrimary (citable) accession number: Q27783
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3