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Q27783

- DRTS_TRYBB

UniProt

Q27783 - DRTS_TRYBB

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Protein
Bifunctional dihydrofolate reductase-thymidylate synthase
Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321Substrate; via carbonyl oxygen By similarity
Binding sitei34 – 341NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei54 – 541Substrate By similarity
Binding sitei160 – 1601Substrate; via carbonyl oxygen By similarity
Binding sitei166 – 1661Substrate By similarity
Binding sitei184 – 1841Substrate By similarity
Binding sitei263 – 2631dUMP By similarity
Active sitei409 – 4091 By similarity
Binding sitei410 – 4101dUMP By similarity
Binding sitei440 – 4401dUMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 467NADP By similarity
Nucleotide bindingi84 – 863NADP By similarity
Nucleotide bindingi105 – 1084NADP By similarity
Nucleotide bindingi161 – 1688NADP By similarity
Nucleotide bindingi428 – 4325dUMP By similarity
Nucleotide bindingi470 – 4723dUMP By similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
  3. glycine biosynthetic process Source: InterPro
  4. one-carbon metabolic process Source: UniProtKB-KW
  5. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.
UPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
PRO_0000186353Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 368
Beta strandi40 – 467
Helixi52 – 6312
Beta strandi66 – 694
Beta strandi76 – 838
Helixi84 – 896
Helixi92 – 943
Beta strandi100 – 1056
Helixi111 – 1155
Helixi121 – 1277
Helixi128 – 1303
Beta strandi131 – 1366
Helixi138 – 1447
Helixi148 – 1536
Beta strandi154 – 1596
Helixi163 – 1697
Helixi174 – 1774
Beta strandi178 – 18811
Beta strandi212 – 2176
Beta strandi230 – 2389

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QFXX-ray2.20A/B1-241[»]
3RG9X-ray2.00A/B1-240[»]
ProteinModelPortaliQ27783.
SMRiQ27783. Positions 11-521.

Miscellaneous databases

EvolutionaryTraceiQ27783.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 238211DHFR
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 527285Thymidylate synthaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.
In the C-terminal section; belongs to the thymidylate synthase family.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27783-1 [UniParc]FASTAAdd to Basket

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MLSLTRILRK KIPVHELAGK ISRPPLRPFS VVVASDEKGG IGDGGTIPWE    50
IPEDMQYFRR VTTNLRGKNV KPSPSKRNAV VMGRKTWDSL PPKFRPLSNR 100
LNVVLSRSAT KEQLLAGIPD PIKRAEAAND VVAVNGGLED ALRMLVSKEH 150
TSSIETVFCI GGGTIYKQAL CAPCVNVLQA IHRTVVRPAS NSCSVFFDIP 200
AAGTKTPEGL ELVRESITDE RVSTGAGGKK YQFEKLVPRN SEEEQYLNLV 250
GRIIDEGCTK CDRTGVGTRS LFGAQMRFSL RNNRLPLLTT KRVFWRGVCE 300
ELLWFLRGET NAKLLSDKGI HIWDGNGSRA FLDSRGLTDY DEMDLGPVYG 350
FQWRHFGADY ISCKVDSEGK GVDQIANIVK SLIENPDDRR MICTAWNPAA 400
LPRMALPPCH MMAQFYVSNG ELSCMLYQRS CDMGLGVPFN IASYALLTFL 450
MAKASGLRPG ELVHTLGDAH VYSNHVEPCR KQLKRVPRPF PFIVFKQDKE 500
FLEDFQESDI EVIDYSPYPV ISMEMAV 527
Length:527
Mass (Da):58,805
Last modified:November 1, 1996 - v1
Checksum:i39982FC553BF7601
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20781 Genomic DNA. Translation: AAA91362.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20781 Genomic DNA. Translation: AAA91362.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3QFX X-ray 2.20 A/B 1-241 [» ]
3RG9 X-ray 2.00 A/B 1-240 [» ]
ProteinModelPortali Q27783.
SMRi Q27783. Positions 11-521.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
UPA00575 .

Miscellaneous databases

EvolutionaryTracei Q27783.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme."
    Gamarro F., Yu P.L., Zhao J., Edman U., Greene P.J., Santi D.
    Mol. Biochem. Parasitol. 72:11-22(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 427.

Entry informationi

Entry nameiDRTS_TRYBB
AccessioniPrimary (citable) accession number: Q27783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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