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Q27778

- PFKA_SCHMA

UniProt

Q27778 - PFKA_SCHMA

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Protein

ATP-dependent 6-phosphofructokinase

Gene
PFK
Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.2 Publications

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.1 Publication

Kineticsi

  1. KM=3.5 mM for ATP1 Publication
  2. KM=0.8 mM for fructose 6-phosphate

pH dependencei

Optimum pH is 7.5-9.0.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271ATP; via amide nitrogen By similarity
Metal bindingi121 – 1211Magnesium; catalytic By similarity
Active sitei168 – 1681Proton acceptor By similarity
Binding sitei203 – 2031Substrate; shared with dimeric partner By similarity
Binding sitei266 – 2661Substrate By similarity
Binding sitei294 – 2941Substrate; shared with dimeric partner By similarity
Binding sitei479 – 4791Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei575 – 5751Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei638 – 6381Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei664 – 6641Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei745 – 7451Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 912ATP By similarity
Nucleotide bindingi120 – 1234ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ27778.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:PFK
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 781781ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000112033Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ27778.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 394394N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni166 – 1683Substrate binding By similarity
Regioni210 – 2123Substrate binding By similarity
Regioni300 – 3034Substrate binding By similarity
Regioni395 – 40915Interdomain linkerUniRule annotationAdd
BLAST
Regioni410 – 781372C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni537 – 5415Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni582 – 5843Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni670 – 6734Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27778-1 [UniParc]FASTAAdd to Basket

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MATWMEGKYV ARGQFTGECI AVLTSGGDAQ GMNAAVRAVV RMGIYCGCRV    50
FFIREGYQGL VDGGQNIQEA SWADVSGILQ LGGTKIGSAR CMDFRERYGR 100
LKAAENLVKN QITNLVVIGG DGSLTGANLF RAEWSSLLEE LVTSNKISAE 150
SAKQFHRLNI VGLVGSIDND FCGTDMTIGA DSALHRIIEA TDAISTTAHS 200
HQRCFILEVM GRHCGYLALV ASMACEADWV FIPEMPPTDD WREKLCHKLR 250
MNREHGQRVN IIMVAEGAID RACKPITCEI VKNLIVSELQ LDTRITVLGH 300
VQRGGSPSAF DRILGSRMGA EAVLALMDAD RDPNLPSCVI SLDGNQAVRV 350
PLVKCVDRTR QVAEAMKACD FDHAVELRGT SFMNNLATYI KLSKIEQPRQ 400
SVMSSENNLR IGIVNVGAPA CGINAVIRGF TRLGITKGYK VIGIHEGFSG 450
LVKGDASEIQ WADVRGWVGM GGSMLGTRRD TPNGLGIDKV AAKFKELKLS 500
GLLIIGGFEA YECMIELVEG REKYPELCIP MAMVPATISN NVPGTDFSLG 550
CDTALNEITS VLDKIKQSAL GTKRRVFVVE TMGGYCGYLA TMSALAGGAD 600
AAYIFEEPFT IDDLREDVVH LRAKIDDNVK RGLVLRADML INTITSEFIH 650
QLYAQEGQGI FDCRCNVLGH MQQGDRPSPF DRSLGTKFAS KAIDWLDEQI 700
NANIVQILQS IHQTFMLYIN WYCTSSDNLF KYSLELREHT DFVHRLPKEE 750
WWLSLRPLMR IMAKHDSLYE SESIMAGTDR K 781
Length:781
Mass (Da):86,060
Last modified:November 1, 1996 - v1
Checksum:iB2CDF20CAE926C38
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L31531 mRNA. Translation: AAA29911.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L31531 mRNA. Translation: AAA29911.1 .

3D structure databases

ProteinModelPortali Q27778.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK Q27778.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a cDNA encoding phosphofructokinase from Schistosoma mansoni."
    Ding J., Su J.G.J., Mansour T.E.
    Mol. Biochem. Parasitol. 66:105-110(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY.
  2. "Purification, kinetics and inhibition by antimonials of recombinant phosphofructokinase from Schistosoma mansoni."
    Su J.G., Mansour J.M., Mansour T.E.
    Mol. Biochem. Parasitol. 81:171-178(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.

Entry informationi

Entry nameiPFKA_SCHMA
AccessioniPrimary (citable) accession number: Q27778
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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