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Protein

C-1-tetrahydrofolate synthase, cytoplasmic

Gene
N/A
Organism
Spodoptera frugiperda (Fall armyworm)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei195NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi170 – 172NADPBy similarity3
Nucleotide bindingi378 – 385ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
C-1-tetrahydrofolate synthase, cytoplasmic
Short name:
C1-THF synthase
Including the following 3 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Formyltetrahydrofolate synthetase (EC:6.3.4.3)
OrganismiSpodoptera frugiperda (Fall armyworm)
Taxonomic identifieri7108 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaLepidopteraGlossataDitrysiaNoctuoideaNoctuidaeAmphipyrinaeSpodoptera

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001993251 – 933C-1-tetrahydrofolate synthase, cytoplasmicAdd BLAST933

Proteomic databases

PRIDEiQ27772

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ27772
SMRiQ27772
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 303Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST303
Regioni51 – 55Substrate bindingBy similarity5
Regioni98 – 100Substrate bindingBy similarity3
Regioni270 – 274Substrate bindingBy similarity5
Regioni304 – 933Formyltetrahydrofolate synthetaseAdd BLAST630

Domaini

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Family and domain databases

CDDicd00477 FTHFS, 1 hit
HAMAPiMF_01543 FTHFS, 1 hit
MF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR000559 Formate_THF_ligase
IPR020628 Formate_THF_ligase_CS
IPR036291 NAD(P)-bd_dom_sf
IPR027417 P-loop_NTPase
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PfamiView protein in Pfam
PF01268 FTHFS, 1 hit
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00721 FTHFS_1, 1 hit
PS00722 FTHFS_2, 1 hit
PS00766 THF_DHG_CYH_1, 1 hit
PS00767 THF_DHG_CYH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q27772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWEPQGSLDP VLESIENELR DQVAEARSRW AGFDARLAIV QVGGREDSNV
60 70 80 90 100
YIRMKLRAAE NIGITAEHIQ LPRDITETEL LANITSLNES PYVHGIIVQM
110 120 130 140 150
PLDSVHPIDS HAITDAVSPD KDVDGLNTIN EGRVRVGDLS GFIPCTPAGC
160 170 180 190 200
VELIKRTGVT IAGKNVVVLG RSRIVGTPVS ELLKWEHATV TVCHSKTKNL
210 220 230 240 250
SEITKTADIL VVAIGRGEMV RGSWIKPGAV VIDCGINPIS DPTKKSGQRL
260 270 280 290 300
VGDVAYEEAV QVASHVTPVP GGVGPMTVAM LMRNTVQAAR RQLDRLLAPT
310 320 330 340 350
WPLRPLRITP LSPPPSDIVI ARSQKPKDIS ELAHEIGLFS NEVSQYGRTK
360 370 380 390 400
AKISLSVLDR MRNQQGAKYI VVAGITPTPL GEGKSTTLLG LVQALSAHRG
410 420 430 440 450
RNSFAVMRQP SQGPTFGVKG GAAGGGYSQV IPMEEFNLHL TGDIHAGTAA
460 470 480 490 500
NNLLAAQMDA RIFHELTQKD GPLFDRLVPK IKGVRKFSPI QLRRLKRLGI
510 520 530 540 550
TKTDPDTLTE GEKSKFARLN IDTSKIMWNR VVDLNDRYLR KITVGQSPTE
560 570 580 590 600
KGFTRETAFD ISVASEIMAI LALGRDVEDI KERLANMVVA LDKSGNPVTA
610 620 630 640 650
DDLGMTGALL VLLRDAFEPT LMQSLEGTPV LVHTGPFANI RHGCSSILAD
660 670 680 690 700
KIAMKLAGEN GYVATEAGFG SDIGMEKFFD IKCRASGDTP HCAVIVSTVR
710 720 730 740 750
ALKMHGGGPP VSAGMPLNDV YVQENLELLS KGLCNLGKHI SNGNKFGVPV
760 770 780 790 800
VVAINKHGND TPAELNLVKE FAVKNGAFRA VLCDHWAKGG LGALELADAV
810 820 830 840 850
IEACDSKSKF DFLYPLQLSI QEKIQIIAKE MYGAGQVEYT DEVLEKIKTF
860 870 880 890 900
TDMGYDKFPI CMAKTSNSLT GDPAVKGAPT GFTLKINGIF ASVGAGFVVP
910 920 930
MVGEISKMPG LPTRPSIYDI DLNTKTGEIE GLF
Length:933
Mass (Da):100,618
Last modified:January 16, 2004 - v3
Checksum:i55C3FA820C1A55CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36189 mRNA Translation: AAA74302.1
PIRiS53523

Similar proteinsi

Entry informationi

Entry nameiC1TC_SPOFR
AccessioniPrimary (citable) accession number: Q27772
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: May 23, 2018
This is version 95 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

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