Reviewed, UniProtKB/Swiss-Prot Q27757 (LUCI_PHOPE)

Names and origin

Protein names	Recommended name:     Luciferin 4-monooxygenase       Short name=Luciferase     EC=1.13.12.7
Organism	Photuris pennsylvanica (Pennsylvania firefly)
Taxonomic identifier	41716 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Elateriformia › Elateroidea › Lampyridae › Photurinae › Photuris

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces green light with a wavelength of 562 nm By similarity.
Catalytic activity	Photinus luciferin + O(2) + ATP = oxidized Photinus luciferin + CO(2) + AMP + diphosphate + light.
Cofactor	Magnesium By similarity.
Subcellular location	PeroxisomeBy similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Luminescence
Cellular component	Peroxisome
Ligand	Magnesium Metal-binding
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Luciferin 4-monooxygenase		PRO_0000193146
Regions
	Motif	543 – 545	3	Microbody targeting signal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q27757-1 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: F0FE4E828047C26E	FASTA	545	60,649
	10 20 30 40 50 60 MEDKNILYGP EPFHPLADGT AGEQMFYALS RYADISGCIA LTNAHTKENV LYEEFLKLSC 70 80 90 100 110 120 RLAESFKKYG LKQNDTIAVC SENGLQFFLP LIASLYLGII AAPVSDKYIE RELIHSLGIV 130 140 150 160 170 180 KPRIIFCSKN TFQKVLNVKS KLKYVETIII LDLNEDLGGY QCLNNFISQN SDINLDVKKF 190 200 210 220 230 240 KPNSFNRDDQ VALVMFSSGT TGVSKGVMLT HKNIVARFSH CKDPTFGNAI NPTTAILTVI 250 260 270 280 290 300 PFHHGFGMTT TLGYFTCGFR VALMHTFEEK LFLQSLQDYK VESTLLVPTL MAFFPKSALV 310 320 330 340 350 360 EKYDLSHLKE IASGGAPLSK EIGEMVKKRF KLNFVRQGYG LTETTSAVLI TPDTDVRPGS 370 380 390 400 410 420 TGKIVPFHAV KVVDPTTGKI LGPNETGELY FKGDMIMKSY YNNEEATKAI INKDGWLRSG 430 440 450 460 470 480 DIAYYDNDGH FYIVDRLKSL IKYKGYQVAP AEIEGILLQH PYIVDAGVTG IPDEAAGELP 490 500 510 520 530 540 AAGVVVQTGK YLNEQIVQNF VSSQVSTAKW LRGGVKFLDE IPKGSTGKID RKVLRQMFEK HKSKL

References

[1]	"Cloning and sequencing of a cDNA for firefly luciferase from Photuris pennsylvanica." Ye L., Buck L.M., Schaeffer H.J., Leach F.R. Biochim. Biophys. Acta 1339:39-52(1997) [PubMed: 9165098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lantern.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31240 mRNA. Translation: AAB60897.1.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PRINTS	PR00154. AMPBINDING.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUCI_PHOPE

Accession

Primary (citable) accession number: Q27757
Secondary accession number(s): O02653

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	January 1, 1998
Last modified:	November 4, 2008
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)