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Q27756

- CP6B3_PAPPO

UniProt

Q27756 - CP6B3_PAPPO

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Protein

Cytochrome P450 6B3

Gene
CYP6B3
Organism
Papilio polyxenes (Black swallowtail butterfly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi443 – 4431Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 6B3 (EC:1.14.14.1)
Alternative name(s):
CYP6B3v1/CYP6B3v2
CYPVIB3
Gene namesi
Name:CYP6B3
OrganismiPapilio polyxenes (Black swallowtail butterfly)
Taxonomic identifieri7146 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Cytochrome P450 6B3PRO_0000051895Add
BLAST

Expressioni

Inductioni

By xanthotoxin and bergapten (linear furanocoumarins) as well as by angelicin and sphondin (angular furanocoumarins).

Structurei

3D structure databases

ProteinModelPortaliQ27756.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27756-1 [UniParc]FASTAAdd to Basket

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MLYVITLVTV LAGLLHYYFT RNFDYWKKRN VAGPKPIPFF GNLKDSALRR    50
KPQVMVYKSI YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VPTKDFEPFA 100
DRGVEFSLDG LGANIFHADG DRWRSLRNRF TPLFTSGKLK TMLPLMSQVG 150
DKFINTSDEV SQTKSEQSIH DLVQIFTITN IAACAFGLNL DENMLKTLQD 200
LDKYIFTVNY SGEFDMLYPG ILKKFNGSIF PKVVKQFFDK LTKDIFEMRK 250
GTSSCQKDMI DSIQELRQQK TVDLWRKHDN EDVKPLELTD GVISAQMFIF 300
YAAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLG 350
EMTFLGRVFD ETLRKYPVGD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW 400
GIQNDPKYYP NPEKFDPERF NPENIKNRHP CAYLPFSAGP RNCLGLRFAK 450
WQIEVCVVKV LSKYRVEPSN KSSGEFKFDP MRLFVLPKGG IFVNIVRR 498
Length:498
Mass (Da):57,473
Last modified:November 1, 1996 - v1
Checksum:i9BC760ACBEB657BC
GO

Polymorphismi

The sequence shown is that of 6B3-1, 6B3-2 seems to differ in 17 positions and is probably an allele.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti47 – 471A → V in 6B3-2.
Natural varianti52 – 521P → H in 6B3-2.
Natural varianti82 – 821L → I in 6B3-2.
Natural varianti92 – 932PT → LI in 6B3-2.
Natural varianti98 – 981P → S in 6B3-2.
Natural varianti108 – 1081L → I in 6B3-2.
Natural varianti289 – 2891T → I in 6B3-2.
Natural varianti350 – 3501G → S in 6B3-2.
Natural varianti354 – 3574FLGR → YLSK in 6B3-2.
Natural varianti395 – 3973IIV → VII in 6B3-2.
Natural varianti401 – 4011G → S in 6B3-2.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25819 mRNA. Translation: AAA96255.1.
U65488 Genomic DNA. Translation: AAB06741.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25819 mRNA. Translation: AAA96255.1 .
U65488 Genomic DNA. Translation: AAB06741.1 .

3D structure databases

ProteinModelPortali Q27756.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CYP6B3: a second furanocoumarin-inducible cytochrome P450 expressed in Papilio polyxenes."
    Hung C.F., Harrison T.L., Berenbaum M.R., Schuler M.A.
    Insect Mol. Biol. 4:149-160(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CYP6B3V1).
  2. "Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification."
    Hung C.F., Holzmacher R., Connolly E., Berenbaum M.R., Schuler M.A.
    Proc. Natl. Acad. Sci. U.S.A. 93:12200-12205(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B3V2).

Entry informationi

Entry nameiCP6B3_PAPPO
AccessioniPrimary (citable) accession number: Q27756
Secondary accession number(s): Q95038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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