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Q27756

- CP6B3_PAPPO

UniProt

Q27756 - CP6B3_PAPPO

Protein

Cytochrome P450 6B3

Gene

CYP6B3

Organism
Papilio polyxenes (Black swallowtail butterfly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi443 – 4431Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 6B3 (EC:1.14.14.1)
    Alternative name(s):
    CYP6B3v1/CYP6B3v2
    CYPVIB3
    Gene namesi
    Name:CYP6B3
    OrganismiPapilio polyxenes (Black swallowtail butterfly)
    Taxonomic identifieri7146 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498Cytochrome P450 6B3PRO_0000051895Add
    BLAST

    Expressioni

    Inductioni

    By xanthotoxin and bergapten (linear furanocoumarins) as well as by angelicin and sphondin (angular furanocoumarins).

    Structurei

    3D structure databases

    ProteinModelPortaliQ27756.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27756-1 [UniParc]FASTAAdd to Basket

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    MLYVITLVTV LAGLLHYYFT RNFDYWKKRN VAGPKPIPFF GNLKDSALRR    50
    KPQVMVYKSI YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VPTKDFEPFA 100
    DRGVEFSLDG LGANIFHADG DRWRSLRNRF TPLFTSGKLK TMLPLMSQVG 150
    DKFINTSDEV SQTKSEQSIH DLVQIFTITN IAACAFGLNL DENMLKTLQD 200
    LDKYIFTVNY SGEFDMLYPG ILKKFNGSIF PKVVKQFFDK LTKDIFEMRK 250
    GTSSCQKDMI DSIQELRQQK TVDLWRKHDN EDVKPLELTD GVISAQMFIF 300
    YAAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLG 350
    EMTFLGRVFD ETLRKYPVGD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW 400
    GIQNDPKYYP NPEKFDPERF NPENIKNRHP CAYLPFSAGP RNCLGLRFAK 450
    WQIEVCVVKV LSKYRVEPSN KSSGEFKFDP MRLFVLPKGG IFVNIVRR 498
    Length:498
    Mass (Da):57,473
    Last modified:November 1, 1996 - v1
    Checksum:i9BC760ACBEB657BC
    GO

    Polymorphismi

    The sequence shown is that of 6B3-1, 6B3-2 seems to differ in 17 positions and is probably an allele.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti47 – 471A → V in 6B3-2.
    Natural varianti52 – 521P → H in 6B3-2.
    Natural varianti82 – 821L → I in 6B3-2.
    Natural varianti92 – 932PT → LI in 6B3-2.
    Natural varianti98 – 981P → S in 6B3-2.
    Natural varianti108 – 1081L → I in 6B3-2.
    Natural varianti289 – 2891T → I in 6B3-2.
    Natural varianti350 – 3501G → S in 6B3-2.
    Natural varianti354 – 3574FLGR → YLSK in 6B3-2.
    Natural varianti395 – 3973IIV → VII in 6B3-2.
    Natural varianti401 – 4011G → S in 6B3-2.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25819 mRNA. Translation: AAA96255.1.
    U65488 Genomic DNA. Translation: AAB06741.1.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25819 mRNA. Translation: AAA96255.1 .
    U65488 Genomic DNA. Translation: AAB06741.1 .

    3D structure databases

    ProteinModelPortali Q27756.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CYP6B3: a second furanocoumarin-inducible cytochrome P450 expressed in Papilio polyxenes."
      Hung C.F., Harrison T.L., Berenbaum M.R., Schuler M.A.
      Insect Mol. Biol. 4:149-160(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CYP6B3V1).
    2. "Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification."
      Hung C.F., Holzmacher R., Connolly E., Berenbaum M.R., Schuler M.A.
      Proc. Natl. Acad. Sci. U.S.A. 93:12200-12205(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B3V2).

    Entry informationi

    Entry nameiCP6B3_PAPPO
    AccessioniPrimary (citable) accession number: Q27756
    Secondary accession number(s): Q95038
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3