Q27756 (CP6B3_PAPPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 6B3
EC=1.14.14.1

Alternative name(s):
CYP6B3v1/CYP6B3v2
CYPVIB3

Gene names

Name:

CYP6B3

Organism

Papilio polyxenes (Black swallowtail butterfly)

Taxonomic identifier

7146 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Papilionoidea › Papilionidae › Papilioninae › Papilionini › Papilio

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By xanthotoxin and bergapten (linear furanocoumarins) as well as by angelicin and sphondin (angular furanocoumarins).
Polymorphism	The sequence shown is that of 6B3-1, 6B3-2 seems to differ in 17 positions and is probably an allele.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Cytochrome P450 6B3		PRO_0000051895
Sites
	Metal binding	443	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	47	1	A → V in 6B3-2.
	Natural variant	52	1	P → H in 6B3-2.
	Natural variant	82	1	L → I in 6B3-2.
	Natural variant	92 – 93	2	PT → LI in 6B3-2.
	Natural variant	98	1	P → S in 6B3-2.
	Natural variant	108	1	L → I in 6B3-2.
	Natural variant	289	1	T → I in 6B3-2.
	Natural variant	350	1	G → S in 6B3-2.
	Natural variant	354 – 357	4	FLGR → YLSK in 6B3-2.
	Natural variant	395 – 397	3	IIV → VII in 6B3-2.
	Natural variant	401	1	G → S in 6B3-2.

Sequences

	Sequence		Length	Mass (Da)
	Q27756 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9BC760ACBEB657BC	FASTA	498	57,473
	10 20 30 40 50 60 MLYVITLVTV LAGLLHYYFT RNFDYWKKRN VAGPKPIPFF GNLKDSALRR KPQVMVYKSI 70 80 90 100 110 120 YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VPTKDFEPFA DRGVEFSLDG LGANIFHADG 130 140 150 160 170 180 DRWRSLRNRF TPLFTSGKLK TMLPLMSQVG DKFINTSDEV SQTKSEQSIH DLVQIFTITN 190 200 210 220 230 240 IAACAFGLNL DENMLKTLQD LDKYIFTVNY SGEFDMLYPG ILKKFNGSIF PKVVKQFFDK 250 260 270 280 290 300 LTKDIFEMRK GTSSCQKDMI DSIQELRQQK TVDLWRKHDN EDVKPLELTD GVISAQMFIF 310 320 330 340 350 360 YAAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLG EMTFLGRVFD 370 380 390 400 410 420 ETLRKYPVGD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW GIQNDPKYYP NPEKFDPERF 430 440 450 460 470 480 NPENIKNRHP CAYLPFSAGP RNCLGLRFAK WQIEVCVVKV LSKYRVEPSN KSSGEFKFDP 490 MRLFVLPKGG IFVNIVRR

References

[1]	"CYP6B3: a second furanocoumarin-inducible cytochrome P450 expressed in Papilio polyxenes." Hung C.F., Harrison T.L., Berenbaum M.R., Schuler M.A. Insect Mol. Biol. 4:149-160(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CYP6B3V1).
[2]	"Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification." Hung C.F., Holzmacher R., Connolly E., Berenbaum M.R., Schuler M.A. Proc. Natl. Acad. Sci. U.S.A. 93:12200-12205(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B3V2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25819 mRNA. Translation: AAA96255.1. U65488 Genomic DNA. Translation: AAB06741.1.
3D structure databases
ProteinModelPortal	Q27756.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP6B3_PAPPO

Accession

Primary (citable) accession number: Q27756
Secondary accession number(s): Q95038

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)