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Q27756 (CP6B3_PAPPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 6B3

EC=1.14.14.1
Alternative name(s):
CYP6B3v1/CYP6B3v2
CYPVIB3
Gene names
Name:CYP6B3
OrganismPapilio polyxenes (Black swallowtail butterfly)
Taxonomic identifier7146 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By xanthotoxin and bergapten (linear furanocoumarins) as well as by angelicin and sphondin (angular furanocoumarins).

Polymorphism

The sequence shown is that of 6B3-1, 6B3-2 seems to differ in 17 positions and is probably an allele.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Cytochrome P450 6B3
PRO_0000051895

Sites

Metal binding4431Iron (heme axial ligand) By similarity

Natural variations

Natural variant471A → V in 6B3-2.
Natural variant521P → H in 6B3-2.
Natural variant821L → I in 6B3-2.
Natural variant92 – 932PT → LI in 6B3-2.
Natural variant981P → S in 6B3-2.
Natural variant1081L → I in 6B3-2.
Natural variant2891T → I in 6B3-2.
Natural variant3501G → S in 6B3-2.
Natural variant354 – 3574FLGR → YLSK in 6B3-2.
Natural variant395 – 3973IIV → VII in 6B3-2.
Natural variant4011G → S in 6B3-2.

Sequences

Sequence LengthMass (Da)Tools
Q27756 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9BC760ACBEB657BC

FASTA49857,473
        10         20         30         40         50         60 
MLYVITLVTV LAGLLHYYFT RNFDYWKKRN VAGPKPIPFF GNLKDSALRR KPQVMVYKSI 

        70         80         90        100        110        120 
YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VPTKDFEPFA DRGVEFSLDG LGANIFHADG 

       130        140        150        160        170        180 
DRWRSLRNRF TPLFTSGKLK TMLPLMSQVG DKFINTSDEV SQTKSEQSIH DLVQIFTITN 

       190        200        210        220        230        240 
IAACAFGLNL DENMLKTLQD LDKYIFTVNY SGEFDMLYPG ILKKFNGSIF PKVVKQFFDK 

       250        260        270        280        290        300 
LTKDIFEMRK GTSSCQKDMI DSIQELRQQK TVDLWRKHDN EDVKPLELTD GVISAQMFIF 

       310        320        330        340        350        360 
YAAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLG EMTFLGRVFD 

       370        380        390        400        410        420 
ETLRKYPVGD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW GIQNDPKYYP NPEKFDPERF 

       430        440        450        460        470        480 
NPENIKNRHP CAYLPFSAGP RNCLGLRFAK WQIEVCVVKV LSKYRVEPSN KSSGEFKFDP 

       490 
MRLFVLPKGG IFVNIVRR 

« Hide

References

[1]"CYP6B3: a second furanocoumarin-inducible cytochrome P450 expressed in Papilio polyxenes."
Hung C.F., Harrison T.L., Berenbaum M.R., Schuler M.A.
Insect Mol. Biol. 4:149-160(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CYP6B3V1).
[2]"Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification."
Hung C.F., Holzmacher R., Connolly E., Berenbaum M.R., Schuler M.A.
Proc. Natl. Acad. Sci. U.S.A. 93:12200-12205(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B3V2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25819 mRNA. Translation: AAA96255.1.
U65488 Genomic DNA. Translation: AAB06741.1.

3D structure databases

ProteinModelPortalQ27756.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP6B3_PAPPO
AccessionPrimary (citable) accession number: Q27756
Secondary accession number(s): Q95038
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families