Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q27756

- CP6B3_PAPPO

UniProt

Q27756 - CP6B3_PAPPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cytochrome P450 6B3

Gene

CYP6B3

Organism
Papilio polyxenes (Black swallowtail butterfly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi443 – 4431Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 6B3 (EC:1.14.14.1)
Alternative name(s):
CYP6B3v1/CYP6B3v2
CYPVIB3
Gene namesi
Name:CYP6B3
OrganismiPapilio polyxenes (Black swallowtail butterfly)
Taxonomic identifieri7146 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Cytochrome P450 6B3PRO_0000051895Add
BLAST

Expressioni

Inductioni

By xanthotoxin and bergapten (linear furanocoumarins) as well as by angelicin and sphondin (angular furanocoumarins).

Structurei

3D structure databases

ProteinModelPortaliQ27756.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27756-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLYVITLVTV LAGLLHYYFT RNFDYWKKRN VAGPKPIPFF GNLKDSALRR
60 70 80 90 100
KPQVMVYKSI YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VPTKDFEPFA
110 120 130 140 150
DRGVEFSLDG LGANIFHADG DRWRSLRNRF TPLFTSGKLK TMLPLMSQVG
160 170 180 190 200
DKFINTSDEV SQTKSEQSIH DLVQIFTITN IAACAFGLNL DENMLKTLQD
210 220 230 240 250
LDKYIFTVNY SGEFDMLYPG ILKKFNGSIF PKVVKQFFDK LTKDIFEMRK
260 270 280 290 300
GTSSCQKDMI DSIQELRQQK TVDLWRKHDN EDVKPLELTD GVISAQMFIF
310 320 330 340 350
YAAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLG
360 370 380 390 400
EMTFLGRVFD ETLRKYPVGD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW
410 420 430 440 450
GIQNDPKYYP NPEKFDPERF NPENIKNRHP CAYLPFSAGP RNCLGLRFAK
460 470 480 490
WQIEVCVVKV LSKYRVEPSN KSSGEFKFDP MRLFVLPKGG IFVNIVRR
Length:498
Mass (Da):57,473
Last modified:November 1, 1996 - v1
Checksum:i9BC760ACBEB657BC
GO

Polymorphismi

The sequence shown is that of 6B3-1, 6B3-2 seems to differ in 17 positions and is probably an allele.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti47 – 471A → V in 6B3-2.
Natural varianti52 – 521P → H in 6B3-2.
Natural varianti82 – 821L → I in 6B3-2.
Natural varianti92 – 932PT → LI in 6B3-2.
Natural varianti98 – 981P → S in 6B3-2.
Natural varianti108 – 1081L → I in 6B3-2.
Natural varianti289 – 2891T → I in 6B3-2.
Natural varianti350 – 3501G → S in 6B3-2.
Natural varianti354 – 3574FLGR → YLSK in 6B3-2.
Natural varianti395 – 3973IIV → VII in 6B3-2.
Natural varianti401 – 4011G → S in 6B3-2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25819 mRNA. Translation: AAA96255.1.
U65488 Genomic DNA. Translation: AAB06741.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25819 mRNA. Translation: AAA96255.1 .
U65488 Genomic DNA. Translation: AAB06741.1 .

3D structure databases

ProteinModelPortali Q27756.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CYP6B3: a second furanocoumarin-inducible cytochrome P450 expressed in Papilio polyxenes."
    Hung C.F., Harrison T.L., Berenbaum M.R., Schuler M.A.
    Insect Mol. Biol. 4:149-160(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CYP6B3V1).
  2. "Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification."
    Hung C.F., Holzmacher R., Connolly E., Berenbaum M.R., Schuler M.A.
    Proc. Natl. Acad. Sci. U.S.A. 93:12200-12205(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B3V2).

Entry informationi

Entry nameiCP6B3_PAPPO
AccessioniPrimary (citable) accession number: Q27756
Secondary accession number(s): Q95038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3