Reviewed, UniProtKB/Swiss-Prot Q27746 (DNMT1_PARLI)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase PliMCI
    EC=2.1.1.37
Alternative name(s):
    Dnmt1
    MCMT
    DNA methyltransferase PliMCI
      Short name=DNA MTase PliMCI
      Short name=M.PliMCI

Gene names

Name:	DNMT
Synonyms:	PLIMCIM

Organism

Paracentrotus lividus (Common sea urchin)

Taxonomic identifier

7656 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Echinodermata › Eleutherozoa › Echinozoa › Echinoidea › Euechinoidea › Echinacea › Echinoida › Echinidae › Paracentrotus

Protein attributes

Sequence length	1612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Methylates CpG residues.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 2 BAH domains. Contains 1 CXXC-type zinc finger.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription factor binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1612	1612	DNA (cytosine-5)-methyltransferase PliMCI		PRO_0000088038
Regions
	Domain	743 – 871	129	BAH 1
	Domain	967 – 1089	123	BAH 2
	Zinc finger	626 – 672	47	CXXC-type
	Compositional bias	172 – 178	7	Poly-Lys
	Compositional bias	288 – 291	4	Poly-Glu
	Compositional bias	303 – 308	6	Poly-Glu
	Compositional bias	712 – 715	4	Poly-Lys
Sites
	Active site	1218	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27746-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0CB3853FB565CF50	FASTA	1,612	181,219
	10 20 30 40 50 60 MPSKTICDQV IPPNVRDRVQ ELDGDLNDGL ITEKGYVKKK SKILFEHLSP DIQTKLKGLE 70 80 90 100 110 120 DELKDEELTE KGYLNKVQSI LAKFIETCSP VNGDTKEEAS SNGKDDEKAE STVANGTTSN 130 140 150 160 170 180 GSTTNGSSGS SKANGHTNGG YVQSSSQEET GTSQSEEEMD MDTPTSGKGG SKKKKKSKGS 190 200 210 220 230 240 GGGDAGKGRK RKVLGDDERD GVEKKEGEKK DVEGEEGEEA KEESATPDEK TLRTSKRKRS 250 260 270 280 290 300 PKADAKQPSI MSMFTKKPAK KEEEKMEESS SMEVDKKEME NGDNGKKEEE EPSGPGGKRI 310 320 330 340 350 360 KKEEEEEEKA KVEPMSPSRD LRHKANHETA ESKQPPLRCK ECRQLLDDPD LKIFPGDPED 370 380 390 400 410 420 AREEYITLTD PRLSLLTGDE GDAMSYDERL QHKITNFCVY DKSTHICAFD RGMIEKNKEL 430 440 450 460 470 480 YFSGYVKPIY DDNPSTEGGI PTKRIGPINE WYTTGFDGGH KALIGFSTAF AEYIVMSPSE 490 500 510 520 530 540 EYKPFWTAVQ EKIYMSKILI EFLQNNVDPV YEDLLTQIET TVPPEGCNRF TEDSLLRHAQ 550 560 570 580 590 600 FVVEQVESYD DAADRDEVLL ITMPCMRDLI KLAGVTLGKR RAARKAAAVK KDKKPVFTMA 610 620 630 640 650 660 TVTPLVSHIF DAIFKDQIAD EMKAAASERK KRCGVCEICQ APDCGKCTAC KDMIKFGGSG 670 680 690 700 710 720 KAKQACKDRR CPNMAVQEAD ENDIDEMDNS SNKENKDEKK AKKGRKLETP LKKKKRAKVT 730 740 750 760 770 780 WLDEPTEVTE ERAYYKAAML DDEKIEIGDC VLIHPDDPTK PLFMARVIYM WQESQGEMMF 790 800 810 820 830 840 HAQWFVYGSE TVLGETSDPL EVFPIDECQD TYLGSVNAKC TVIYKAPPND WSMIGGIDDP 850 860 870 880 890 900 ETDHVIKEDD GKTFFYQKWY DPELARFEDY EVLMAPDDIP AHRFCSCCLK NERAQEKETA 910 920 930 940 950 960 RPGAKLEDQD DSSKVLYSSW HYKGNEFQIG DGVYLLPEVF SFNIKQKVVT KKPVSKKDVD 970 980 990 1000 1010 1020 EDLYPENYRK SSEYVKGSNL ECPEPFRIGK IISIYTTKSN STVRLRVNKM YRPEDTHKGR 1030 1040 1050 1060 1070 1080 TAAYQADLNV LYWSEEEAVT ELEVVQGKCS VVCAEDLNVS TDEYSAGGPH KFYFREAYDS 1090 1100 1110 1120 1130 1140 ERKCFEDPPS KSRSTRMKGK GKGKGKGKAK GKIAVEKEEE KESTETPFNK LKCLDVFAGC 1150 1160 1170 1180 1190 1200 GGLSEGFHQA GICESSWAIE KEEPAAQAYR LNNPGSTVFS DDCNELLRLV MQGEKTSRTG 1210 1220 1230 1240 1250 1260 QKLPQKGDVE LLCGGPPCQG FSGMNRFNSR EYSKFKNSLI SSYLSYCDYY RPRFFLLENV 1270 1280 1290 1300 1310 1320 RNFVSYKKNM VLKLALRCLI RMGYQCTFGI LQAGQYGVPQ TRRRAIILAA APGEKLPFYP 1330 1340 1350 1360 1370 1380 EPLHVFSSRA CSLSVMIGEK KIESNNQWCL SAPYRTITVR DTMSDLPTIN NGAQKLEISY 1390 1400 1410 1420 1430 1440 DGEPQSDFQK KIRGNQYQPI LRDHICKDMS SLVAARMKHI PLAPGSDWRD LPNIPVTLKD 1450 1460 1470 1480 1490 1500 GTTCRKLRYT HKDKKNGKSS TGALRGVCSC AEGDACDPSD RQFSTLIPWC LPHTGNRHNN 1510 1520 1530 1540 1550 1560 WAGLYGRLEW DGFFSTTVTN PEPMGKQGRV LHPEQHRVVS VRECARSQGF PDTYRFFGSI 1570 1580 1590 1600 1610 LDKHRQIGNA VPPPMAAAIG MEIKVCLQTK TKRDQERAAL EPVKEETEES MD

References

[1]	"Isolation of cDNA clones encoding DNA methyltransferase of sea urchin P. lividus: expression during embryonic development." Aniello F., Locascio A., Fucci L., Geraci G., Branno M. Gene 178:57-61(1996) [PubMed: 8921892] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50183 mRNA. Translation: CAA90563.1.
PIR	JC5210.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2961. M.PliMCDnmt1.
Enzyme and pathway databases
BRENDA	2.1.1.37. 39472.
Family and domain databases
InterPro	IPR001025. BAH. IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR010506. DMAP1_bd. IPR017198. DNA_C5-MeTrfase_1. IPR002857. Znf_CXXC. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF01426. BAH. 2 hits. PF06464. DMAP_binding. 1 hit. PF00145. DNA_methylase. 3 hits. PF02008. zf-CXXC. 1 hit. [Graphical view]
PIRSF	PIRSF037404. DNMT1. 1 hit.
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 2 hits. [Graphical view]
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS51038. BAH. 2 hits. PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. PS51058. ZF_CXXC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNMT1_PARLI

Accession

Primary (citable) accession number: Q27746

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)