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Q27743

- LDH_PLAFD

UniProt

Q27743 - LDH_PLAFD

Protein

L-lactate dehydrogenase

Gene
N/A
Organism
Plasmodium falciparum (isolate CDC / Honduras)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-lactate + NAD+ = pyruvate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141NAD; via amide nitrogen
    Binding sitei15 – 151NAD; via amide nitrogen
    Binding sitei35 – 351NAD
    Binding sitei67 – 671NAD
    Binding sitei81 – 811NAD; via amide nitrogen
    Binding sitei82 – 821NAD; via carbonyl oxygen
    Binding sitei95 – 951Substrate
    Binding sitei125 – 1251NAD; via carbonyl oxygen
    Binding sitei127 – 1271NAD
    Binding sitei150 – 1501NAD; via carbonyl oxygen
    Binding sitei158 – 1581Substrate
    Active sitei182 – 1821Proton acceptor
    Binding sitei182 – 1821Substrate

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 150137NADAdd
    BLAST

    GO - Molecular functioni

    1. L-lactate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, Pyruvate

    Enzyme and pathway databases

    SABIO-RKQ27743.
    UniPathwayiUPA00554; UER00611.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-lactate dehydrogenase (EC:1.1.1.27)
    Alternative name(s):
    LDH-P
    OrganismiPlasmodium falciparum (isolate CDC / Honduras)
    Taxonomic identifieri5836 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316L-lactate dehydrogenasePRO_0000168495Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    316
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 105
    Helixi14 – 2512
    Beta strandi30 – 345
    Beta strandi36 – 394
    Helixi40 – 4910
    Helixi51 – 555
    Beta strandi61 – 644
    Helixi67 – 704
    Beta strandi74 – 785
    Helixi90 – 923
    Helixi95 – 984
    Helixi99 – 11618
    Beta strandi120 – 1245
    Beta strandi126 – 1283
    Helixi129 – 14012
    Helixi144 – 1463
    Beta strandi147 – 1493
    Helixi152 – 16716
    Helixi171 – 1733
    Beta strandi178 – 1803
    Helixi190 – 1923
    Helixi200 – 2056
    Helixi211 – 22212
    Helixi224 – 2318
    Beta strandi232 – 2343
    Helixi237 – 25115
    Beta strandi256 – 26611
    Helixi267 – 2693
    Beta strandi271 – 28212
    Beta strandi285 – 2895
    Helixi296 – 31520

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CEQX-ray2.00A1-316[»]
    1CETX-ray2.05A1-316[»]
    1LDGX-ray1.74A1-316[»]
    1T24X-ray1.70A1-316[»]
    1T25X-ray1.90A1-316[»]
    1T26X-ray1.80A1-316[»]
    1T2CX-ray2.01A1-316[»]
    1T2DX-ray1.10A1-316[»]
    1T2EX-ray1.85A1-316[»]
    1U4OX-ray1.70A2-316[»]
    1U4SX-ray2.00A2-316[»]
    1U5AX-ray1.80A2-316[»]
    1U5CX-ray2.65A2-316[»]
    1XIVX-ray1.70A2-316[»]
    2A94X-ray1.50A2-316[»]
    ProteinModelPortaliQ27743.
    SMRiQ27743. Positions 2-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ27743.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. LDH family.Curated

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q27743-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPKAKIVLV GSGMIGGVMA TLIVQKNLGD VVLFDIVKNM PHGKALDTSH    50
    TNVMAYSNCK VSGSNTYDDL AGADVVIVTA GFTKAPGKSD KEWNRDDLLP 100
    LNNKIMIEIG GHIKKNCPNA FIIVVTNPVD VMVQLLHQHS GVPKNKIIGL 150
    GGVLDTSRLK YYISQKLNVC PRDVNAHIVG AHGNKMVLLK RYITVGGIPL 200
    QEFINNKLIS DAELEAIFDR TVNTALEIVN LHASPYVAPA AAIIEMAESY 250
    LKDLKKVLIC STLLEGQYGH SDIFGGTPVV LGANGVEQVI ELQLNSEEKA 300
    KFDEAIAETK RMKALA 316
    Length:316
    Mass (Da):34,108
    Last modified:November 1, 1996 - v1
    Checksum:iD25EB863954B8FC1
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti73 – 731A → S.2 Publications
    Natural varianti96 – 961D → L.2 Publications

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93720 mRNA. Translation: AAA29633.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93720 mRNA. Translation: AAA29633.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CEQ X-ray 2.00 A 1-316 [» ]
    1CET X-ray 2.05 A 1-316 [» ]
    1LDG X-ray 1.74 A 1-316 [» ]
    1T24 X-ray 1.70 A 1-316 [» ]
    1T25 X-ray 1.90 A 1-316 [» ]
    1T26 X-ray 1.80 A 1-316 [» ]
    1T2C X-ray 2.01 A 1-316 [» ]
    1T2D X-ray 1.10 A 1-316 [» ]
    1T2E X-ray 1.85 A 1-316 [» ]
    1U4O X-ray 1.70 A 2-316 [» ]
    1U4S X-ray 2.00 A 2-316 [» ]
    1U5A X-ray 1.80 A 2-316 [» ]
    1U5C X-ray 2.65 A 2-316 [» ]
    1XIV X-ray 1.70 A 2-316 [» ]
    2A94 X-ray 1.50 A 2-316 [» ]
    ProteinModelPortali Q27743.
    SMRi Q27743. Positions 2-316.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q27743.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00554 ; UER00611 .
    SABIO-RK Q27743.

    Miscellaneous databases

    EvolutionaryTracei Q27743.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of Plasmodium falciparum lactate dehydrogenase in Escherichia coli."
      Bzik D.J., Fox B.A., Gonyer K.
      Mol. Biochem. Parasitol. 59:155-166(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design."
      Dunn C., Banfield M., Barker J., Higham C., Moreton K., Turgut-Balik D., Brady L., Holbrook J.J.
      Nat. Struct. Biol. 3:912-915(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS), VARIANTS SER-73 AND LEU-96.
    3. "Chloroquine binds in the cofactor binding site of Plasmodium falciparum lactate dehydrogenase."
      Read J.A., Wilkinson K.W., Tranter R., Sessions R.B., Brady R.L.
      J. Biol. Chem. 274:10213-10218(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), VARIANTS SER-73 AND LEU-96.

    Entry informationi

    Entry nameiLDH_PLAFD
    AccessioniPrimary (citable) accession number: Q27743
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3