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Protein

L-lactate dehydrogenase

Gene
N/A
Organism
Plasmodium falciparum (isolate CDC / Honduras)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14NAD; via amide nitrogen1
Binding sitei15NAD; via amide nitrogen1
Binding sitei35NAD1
Binding sitei67NAD1
Binding sitei81NAD; via amide nitrogen1
Binding sitei82NAD; via carbonyl oxygen1
Binding sitei95Substrate1
Binding sitei125NAD; via carbonyl oxygen1
Binding sitei127NAD1
Binding sitei150NAD; via carbonyl oxygen1
Binding sitei158Substrate1
Active sitei182Proton acceptor1
Binding sitei182Substrate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 150NADAdd BLAST137

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, Pyruvate

Enzyme and pathway databases

BRENDAi1.1.1.27. 4889.
SABIO-RKQ27743.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Alternative name(s):
LDH-P
OrganismiPlasmodium falciparum (isolate CDC / Honduras)
Taxonomic identifieri5836 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001684951 – 316L-lactate dehydrogenaseAdd BLAST316

Interactioni

Subunit structurei

Homotetramer.

Chemistry databases

BindingDBiQ27743.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi14 – 25Combined sources12
Beta strandi30 – 34Combined sources5
Beta strandi36 – 39Combined sources4
Helixi40 – 49Combined sources10
Helixi51 – 55Combined sources5
Beta strandi61 – 64Combined sources4
Helixi67 – 70Combined sources4
Beta strandi74 – 78Combined sources5
Helixi90 – 92Combined sources3
Helixi95 – 97Combined sources3
Helixi98 – 116Combined sources19
Beta strandi120 – 124Combined sources5
Beta strandi126 – 128Combined sources3
Helixi129 – 140Combined sources12
Helixi144 – 146Combined sources3
Beta strandi147 – 150Combined sources4
Helixi152 – 167Combined sources16
Helixi171 – 173Combined sources3
Beta strandi178 – 180Combined sources3
Helixi190 – 192Combined sources3
Helixi200 – 205Combined sources6
Helixi211 – 222Combined sources12
Helixi224 – 230Combined sources7
Beta strandi232 – 234Combined sources3
Helixi237 – 251Combined sources15
Beta strandi256 – 266Combined sources11
Helixi267 – 269Combined sources3
Beta strandi271 – 282Combined sources12
Beta strandi285 – 289Combined sources5
Helixi296 – 316Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CEQX-ray2.00A1-316[»]
1CETX-ray2.05A1-316[»]
1LDGX-ray1.74A1-316[»]
1T24X-ray1.70A1-316[»]
1T25X-ray1.90A1-316[»]
1T26X-ray1.80A1-316[»]
1T2CX-ray2.01A1-316[»]
1T2DX-ray1.10A1-316[»]
1T2EX-ray1.85A1-316[»]
1U4OX-ray1.70A2-316[»]
1U4SX-ray2.00A2-316[»]
1U5AX-ray1.80A2-316[»]
1U5CX-ray2.65A2-316[»]
1XIVX-ray1.70A2-316[»]
2A94X-ray1.50A2-316[»]
4PLZX-ray1.05A1-316[»]
ProteinModelPortaliQ27743.
SMRiQ27743.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ27743.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKAKIVLV GSGMIGGVMA TLIVQKNLGD VVLFDIVKNM PHGKALDTSH
60 70 80 90 100
TNVMAYSNCK VSGSNTYDDL AGADVVIVTA GFTKAPGKSD KEWNRDDLLP
110 120 130 140 150
LNNKIMIEIG GHIKKNCPNA FIIVVTNPVD VMVQLLHQHS GVPKNKIIGL
160 170 180 190 200
GGVLDTSRLK YYISQKLNVC PRDVNAHIVG AHGNKMVLLK RYITVGGIPL
210 220 230 240 250
QEFINNKLIS DAELEAIFDR TVNTALEIVN LHASPYVAPA AAIIEMAESY
260 270 280 290 300
LKDLKKVLIC STLLEGQYGH SDIFGGTPVV LGANGVEQVI ELQLNSEEKA
310
KFDEAIAETK RMKALA
Length:316
Mass (Da):34,108
Last modified:November 1, 1996 - v1
Checksum:iD25EB863954B8FC1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti73A → S.2 Publications1
Natural varianti96D → L.2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93720 mRNA. Translation: AAA29633.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93720 mRNA. Translation: AAA29633.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CEQX-ray2.00A1-316[»]
1CETX-ray2.05A1-316[»]
1LDGX-ray1.74A1-316[»]
1T24X-ray1.70A1-316[»]
1T25X-ray1.90A1-316[»]
1T26X-ray1.80A1-316[»]
1T2CX-ray2.01A1-316[»]
1T2DX-ray1.10A1-316[»]
1T2EX-ray1.85A1-316[»]
1U4OX-ray1.70A2-316[»]
1U4SX-ray2.00A2-316[»]
1U5AX-ray1.80A2-316[»]
1U5CX-ray2.65A2-316[»]
1XIVX-ray1.70A2-316[»]
2A94X-ray1.50A2-316[»]
4PLZX-ray1.05A1-316[»]
ProteinModelPortaliQ27743.
SMRiQ27743.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ27743.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BRENDAi1.1.1.27. 4889.
SABIO-RKQ27743.

Miscellaneous databases

EvolutionaryTraceiQ27743.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_PLAFD
AccessioniPrimary (citable) accession number: Q27743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.