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Q27743

- LDH_PLAFD

UniProt

Q27743 - LDH_PLAFD

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Protein
L-lactate dehydrogenase
Gene
N/A
Organism
Plasmodium falciparum (isolate CDC / Honduras)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141NAD; via amide nitrogen
Binding sitei15 – 151NAD; via amide nitrogen
Binding sitei35 – 351NAD
Binding sitei67 – 671NAD
Binding sitei81 – 811NAD; via amide nitrogen
Binding sitei82 – 821NAD; via carbonyl oxygen
Binding sitei95 – 951Substrate
Binding sitei125 – 1251NAD; via carbonyl oxygen
Binding sitei127 – 1271NAD
Binding sitei150 – 1501NAD; via carbonyl oxygen
Binding sitei158 – 1581Substrate
Active sitei182 – 1821Proton acceptor
Binding sitei182 – 1821Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 150137NAD
Add
BLAST

GO - Molecular functioni

  1. L-lactate dehydrogenase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, Pyruvate

Enzyme and pathway databases

SABIO-RKQ27743.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Alternative name(s):
LDH-P
OrganismiPlasmodium falciparum (isolate CDC / Honduras)
Taxonomic identifieri5836 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316L-lactate dehydrogenase
PRO_0000168495Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105
Helixi14 – 2512
Beta strandi30 – 345
Beta strandi36 – 394
Helixi40 – 4910
Helixi51 – 555
Beta strandi61 – 644
Helixi67 – 704
Beta strandi74 – 785
Helixi90 – 923
Helixi95 – 984
Helixi99 – 11618
Beta strandi120 – 1245
Beta strandi126 – 1283
Helixi129 – 14012
Helixi144 – 1463
Beta strandi147 – 1493
Helixi152 – 16716
Helixi171 – 1733
Beta strandi178 – 1803
Helixi190 – 1923
Helixi200 – 2056
Helixi211 – 22212
Helixi224 – 2318
Beta strandi232 – 2343
Helixi237 – 25115
Beta strandi256 – 26611
Helixi267 – 2693
Beta strandi271 – 28212
Beta strandi285 – 2895
Helixi296 – 31520

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CEQX-ray2.00A1-316[»]
1CETX-ray2.05A1-316[»]
1LDGX-ray1.74A1-316[»]
1T24X-ray1.70A1-316[»]
1T25X-ray1.90A1-316[»]
1T26X-ray1.80A1-316[»]
1T2CX-ray2.01A1-316[»]
1T2DX-ray1.10A1-316[»]
1T2EX-ray1.85A1-316[»]
1U4OX-ray1.70A2-316[»]
1U4SX-ray2.00A2-316[»]
1U5AX-ray1.80A2-316[»]
1U5CX-ray2.65A2-316[»]
1XIVX-ray1.70A2-316[»]
2A94X-ray1.50A2-316[»]
ProteinModelPortaliQ27743.
SMRiQ27743. Positions 2-316.

Miscellaneous databases

EvolutionaryTraceiQ27743.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27743-1 [UniParc]FASTAAdd to Basket

« Hide

MAPKAKIVLV GSGMIGGVMA TLIVQKNLGD VVLFDIVKNM PHGKALDTSH    50
TNVMAYSNCK VSGSNTYDDL AGADVVIVTA GFTKAPGKSD KEWNRDDLLP 100
LNNKIMIEIG GHIKKNCPNA FIIVVTNPVD VMVQLLHQHS GVPKNKIIGL 150
GGVLDTSRLK YYISQKLNVC PRDVNAHIVG AHGNKMVLLK RYITVGGIPL 200
QEFINNKLIS DAELEAIFDR TVNTALEIVN LHASPYVAPA AAIIEMAESY 250
LKDLKKVLIC STLLEGQYGH SDIFGGTPVV LGANGVEQVI ELQLNSEEKA 300
KFDEAIAETK RMKALA 316
Length:316
Mass (Da):34,108
Last modified:November 1, 1996 - v1
Checksum:iD25EB863954B8FC1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti73 – 731A → S.2 Publications
Natural varianti96 – 961D → L.2 Publications

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93720 mRNA. Translation: AAA29633.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93720 mRNA. Translation: AAA29633.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CEQ X-ray 2.00 A 1-316 [» ]
1CET X-ray 2.05 A 1-316 [» ]
1LDG X-ray 1.74 A 1-316 [» ]
1T24 X-ray 1.70 A 1-316 [» ]
1T25 X-ray 1.90 A 1-316 [» ]
1T26 X-ray 1.80 A 1-316 [» ]
1T2C X-ray 2.01 A 1-316 [» ]
1T2D X-ray 1.10 A 1-316 [» ]
1T2E X-ray 1.85 A 1-316 [» ]
1U4O X-ray 1.70 A 2-316 [» ]
1U4S X-ray 2.00 A 2-316 [» ]
1U5A X-ray 1.80 A 2-316 [» ]
1U5C X-ray 2.65 A 2-316 [» ]
1XIV X-ray 1.70 A 2-316 [» ]
2A94 X-ray 1.50 A 2-316 [» ]
ProteinModelPortali Q27743.
SMRi Q27743. Positions 2-316.
ModBasei Search...

Chemistry

BindingDBi Q27743.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00554 ; UER00611 .
SABIO-RK Q27743.

Miscellaneous databases

EvolutionaryTracei Q27743.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
PRINTSi PR00086. LLDHDRGNASE.
SUPFAMi SSF56327. SSF56327. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Expression of Plasmodium falciparum lactate dehydrogenase in Escherichia coli."
    Bzik D.J., Fox B.A., Gonyer K.
    Mol. Biochem. Parasitol. 59:155-166(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design."
    Dunn C., Banfield M., Barker J., Higham C., Moreton K., Turgut-Balik D., Brady L., Holbrook J.J.
    Nat. Struct. Biol. 3:912-915(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS), VARIANTS SER-73 AND LEU-96.
  3. "Chloroquine binds in the cofactor binding site of Plasmodium falciparum lactate dehydrogenase."
    Read J.A., Wilkinson K.W., Tranter R., Sessions R.B., Brady R.L.
    J. Biol. Chem. 274:10213-10218(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), VARIANTS SER-73 AND LEU-96.

Entry informationi

Entry nameiLDH_PLAFD
AccessioniPrimary (citable) accession number: Q27743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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