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Protein

Enolase

Gene

ENO

Organism
Plasmodium falciparum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Binding sitei175SubstrateBy similarity1
Active sitei218Proton donorBy similarity1
Metal bindingi253MagnesiumBy similarity1
Metal bindingi304MagnesiumBy similarity1
Binding sitei304SubstrateBy similarity1
Metal bindingi331MagnesiumBy similarity1
Binding sitei331SubstrateBy similarity1
Active sitei356Proton acceptorBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.1.11. 4889.
SABIO-RKiQ27727.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
OrganismiPlasmodium falciparum
Taxonomic identifieri5833 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340901 – 446EnolaseAdd BLAST446

Proteomic databases

PaxDbiQ27727.
PRIDEiQ27727.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ27727.
SMRiQ27727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni383 – 386Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHVITRINA REILDSRGNP TVEVDLETNL GIFRAAVPSG ASTGIYEALE
60 70 80 90 100
LRDNDKSRYL GKGVQKAIKN INEIIAPKLI GMNCTEQKKI DNLMVEELDG
110 120 130 140 150
SKNEWGWSKS KLGANAILAI SMAVCRAGAA PNKVSLYKYL AQLAGKKSDQ
160 170 180 190 200
MVLPVPCLNV INGGSHAGNK LSFQEFMIVP VGAPSFKEAL RYGAEVYHTL
210 220 230 240 250
KSEIKKKYGI DATNVGDEGG FAPNILNANE ALDLLVTAIK SAGYEGKVKI
260 270 280 290 300
AMDVAASEFY NSENKTYDLD FKTPNNDKSL VKTGAQLVDL YIDLVKKYPI
310 320 330 340 350
VSIEDPFDQD DWENYAKLTA AIGKDVQIVG DDLLVTNPTR ITKALEKNAC
360 370 380 390 400
NALLLKVNQI GSITEAIEAC LLSQKNNWGV MVSHRSGETE DVFIADLVVA
410 420 430 440
LRTGQIKTGA PCRSERNAKY NQLLRIEESL GNNAVFAGEK FRLQLN
Length:446
Mass (Da):48,704
Last modified:November 1, 1996 - v1
Checksum:iC738905EAFB009A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00152 Unassigned DNA. Translation: AAA18634.1.
PIRiS42206.

Entry informationi

Entry nameiENO_PLAFA
AccessioniPrimary (citable) accession number: Q27727
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families