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Q27713

- DRTS_PLABA

UniProt

Q27713 - DRTS_PLABA

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Protein
Bifunctional dihydrofolate reductase-thymidylate synthase
Gene
N/A
Organism
Plasmodium berghei (strain Anka)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511Substrate By similarity
Binding sitei173 – 1731Substrate; via carbonyl oxygen By similarity
Binding sitei179 – 1791Substrate By similarity
Binding sitei194 – 1941Substrate By similarity
Binding sitei324 – 3241dUMP By similarity
Active sitei469 – 4691 By similarity
Binding sitei470 – 4701dUMP By similarity
Binding sitei500 – 5001dUMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 427NADP By similarity
Nucleotide bindingi108 – 1103NADP By similarity
Nucleotide bindingi129 – 1324NADP By similarity
Nucleotide bindingi174 – 1818NADP By similarity
Nucleotide bindingi488 – 4925dUMP By similarity
Nucleotide bindingi530 – 5323dUMP By similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
OrganismiPlasmodium berghei (strain Anka)
Taxonomic identifieri5823 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Vinckeia)

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
PRO_0000186347Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ27713.
SMRiQ27713. Positions 1-238, 264-587.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 237229DHFR
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni301 – 587287Thymidylate synthaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.
In the C-terminal section; belongs to the thymidylate synthase family.

Phylogenomic databases

HOGENOMiHOG000257901.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27713-1 [UniParc]FASTAAdd to Basket

« Hide

MEDLSETFDI YAICACCKVL NDDEKVRCFN NKTFKGIGNA GVLPWKCNLI    50
DMKYFSSVTS YINENNYIRL KWKRDKYMEK HNLKNNVELN TNIISSTNNL 100
QNIVVMGKKS WESIPKKFKP LQNRINIILS RTLKKEDIVN ENNNENNNVI 150
IIKSVDDLFP ILKCTKYYKC FIIGGSSVYK EFLDRNLIKK IYFTRINNSY 200
NCDVLFPEIN ENLFKITSIS DVYYSNNTTL DFIIYSKTKE INPNEEVPNN 250
TFLGVCDEQN KAFDDEDDYT YFSFNKNKEN IKKNSEHAHN FKIYNSIKYK 300
NHPEYQYLNI IYDIIMHGNK QDDRTGVGVL SKFGYMMKFN LNEYFPLLTT 350
KKLFIRGIIE ELLWFIRGET NGNTLLEKNV RIWEANGTRE FLDNRKLFHR 400
EVNDLGPIYG FQWRHFGAEY TDMHDNYKDK GVDQLKNIIN LIKNDPTCRR 450
IILCAWNVKN LDQMALPPCH ILCQFYVFDG KLSCIMYQRS CDLGLGVPFN 500
IASYSIFTYM IAQVCNLQAA EFIHVLGNAH VYNNHIESLK IQLNRTPYPF 550
PTLKLNPDIK NIEDFTISDF TVQNYVHHDK INMDMAA 587
Length:587
Mass (Da):68,932
Last modified:November 1, 1996 - v1
Checksum:i6E638C2B02FEC13A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti110 – 1101S → N in pyrimethamine resistance.
Natural varianti177 – 1771S → F in pyrimethamine resistance.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12275 Genomic DNA. Translation: AAB60237.1.
L28119 Genomic DNA. Translation: AAA29581.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12275 Genomic DNA. Translation: AAB60237.1 .
L28119 Genomic DNA. Translation: AAA29581.1 .

3D structure databases

ProteinModelPortali Q27713.
SMRi Q27713. Positions 1-238, 264-587.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL3963.
DrugBanki DB00440. Trimethoprim.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOGENOMi HOG000257901.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mechanisms of pyrimethamine resistance in two different strains of Plasmodium berghei."
    van Dijk M.R., McConkey G.A., Vinkenoog R., Waters A.P., Janse C.J.
    Mol. Biochem. Parasitol. 68:167-171(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The dihydrofolate reductase domain of rodent malarias: point mutations and pyrimethamine resistance."
    Cheng Q., Saul A.
    Mol. Biochem. Parasitol. 65:361-363(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-201.

Entry informationi

Entry nameiDRTS_PLABA
AccessioniPrimary (citable) accession number: Q27713
Secondary accession number(s): Q27714
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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