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Q27709

- OBL_OBELO

UniProt

Q27709 - OBL_OBELO

Protein

Obelin

Gene
N/A
Organism
Obelia longissima (Black sea hydrozoan) (Laomedea longissima)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ca2+-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO2 with the concomitant emission of light.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi30 – 41121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi123 – 134122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi159 – 170123PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. bioluminescence Source: UniProtKB-KW

    Keywords - Molecular functioni

    Photoprotein

    Keywords - Biological processi

    Luminescence

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Obelin
    Short name:
    OBL
    OrganismiObelia longissima (Black sea hydrozoan) (Laomedea longissima)
    Taxonomic identifieri32570 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaCnidariaHydrozoaHydroidaLeptomedusaeCampanulariidaeObelia

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi92 – 921W → F: Shifts luminescence to violet by adding a new band at 410 nm. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 66Sequence AnalysisPRO_0000004136
    Chaini7 – 195189ObelinPRO_0000004137Add
    BLAST

    Structurei

    Secondary structure

    1
    195
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 53
    Helixi16 – 2914
    Beta strandi33 – 353
    Helixi39 – 4810
    Helixi50 – 534
    Helixi58 – 7417
    Beta strandi82 – 843
    Helixi85 – 10420
    Helixi110 – 12112
    Beta strandi125 – 1317
    Helixi132 – 14211
    Beta strandi143 – 1453
    Helixi148 – 15710
    Beta strandi164 – 1663
    Helixi168 – 17912
    Helixi184 – 1863
    Turni187 – 1926

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EL4X-ray1.73A1-195[»]
    1JF0X-ray1.82A1-195[»]
    1JF2X-ray1.72A1-195[»]
    1QV0X-ray1.10A1-195[»]
    1QV1X-ray1.10A1-195[»]
    1S36X-ray1.96A1-195[»]
    1SL7X-ray2.20A1-195[»]
    1SL9X-ray1.17A1-195[»]
    2F8PX-ray1.93A1-195[»]
    4MRXX-ray1.72A1-195[»]
    4MRYX-ray1.30A1-195[»]
    4N1FX-ray2.09A1-195[»]
    4N1GX-ray1.50A/B1-195[»]
    ProteinModelPortaliQ27709.
    SMRiQ27709. Positions 2-195.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ27709.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 5236EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini53 – 8836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini110 – 14536EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini146 – 18136EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the aequorin family.Curated
    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13405. EF-hand_6. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKYAVKLK TDFDNPRWIK RHKHMFDFLD INGNGKITLD EIVSKASDDI    50
    CAKLEATPEQ TKRHQVCVEA FFRGCGMEYG KEIAFPQFLD GWKQLATSEL 100
    KKWARNEPTL IREWGDAVFD IFDKDGSGTI TLDEWKAYGK ISGISPSQED 150
    CEATFRHCDL DNSGDLDVDE MTRQHLGFWY TLDPEADGLY GNGVP 195
    Length:195
    Mass (Da):22,226
    Last modified:November 1, 1997 - v1
    Checksum:i5D002270B73D3663
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07128 mRNA. Translation: AAA67708.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07128 mRNA. Translation: AAA67708.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EL4 X-ray 1.73 A 1-195 [» ]
    1JF0 X-ray 1.82 A 1-195 [» ]
    1JF2 X-ray 1.72 A 1-195 [» ]
    1QV0 X-ray 1.10 A 1-195 [» ]
    1QV1 X-ray 1.10 A 1-195 [» ]
    1S36 X-ray 1.96 A 1-195 [» ]
    1SL7 X-ray 2.20 A 1-195 [» ]
    1SL9 X-ray 1.17 A 1-195 [» ]
    2F8P X-ray 1.93 A 1-195 [» ]
    4MRX X-ray 1.72 A 1-195 [» ]
    4MRY X-ray 1.30 A 1-195 [» ]
    4N1F X-ray 2.09 A 1-195 [» ]
    4N1G X-ray 1.50 A/B 1-195 [» ]
    ProteinModelPortali Q27709.
    SMRi Q27709. Positions 2-195.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q27709.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13405. EF-hand_6. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima."
      Illarionov B.A., Bondar V.S., Illarionova V.A., Vysotski E.S.
      Gene 153:273-274(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure."
      Liu Z.J., Vysotski E.S., Chen C.J., Rose J.P., Lee J., Wang B.C.
      Protein Sci. 9:2085-2093(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS).
    3. "Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine."
      Liu Z.J., Vysotski E.S., Deng L., Lee J., Rose J., Wang B.C.
      Biochem. Biophys. Res. Commun. 311:433-439(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
    4. "Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence."
      Deng L., Markova S.V., Vysotski E.S., Liu Z.J., Lee J., Rose J., Wang B.C.
      J. Biol. Chem. 279:33647-33652(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS), MUTAGENESIS OF TRP-92.

    Entry informationi

    Entry nameiOBL_OBELO
    AccessioniPrimary (citable) accession number: Q27709
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3