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Q27709

- OBL_OBELO

UniProt

Q27709 - OBL_OBELO

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Protein
Obelin
Gene
N/A
Organism
Obelia longissima (Black sea hydrozoan) (Laomedea longissima)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Ca2+-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO2 with the concomitant emission of light.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi30 – 41121 Reviewed prediction
Add
BLAST
Calcium bindingi123 – 134122 Reviewed prediction
Add
BLAST
Calcium bindingi159 – 170123 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. bioluminescence Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Obelin
Short name:
OBL
OrganismiObelia longissima (Black sea hydrozoan) (Laomedea longissima)
Taxonomic identifieri32570 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaHydrozoaHydroidaLeptomedusaeCampanulariidaeObelia

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi92 – 921W → F: Shifts luminescence to violet by adding a new band at 410 nm. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 66 Reviewed prediction
PRO_0000004136
Chaini7 – 195189Obelin
PRO_0000004137Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 53
Helixi16 – 2914
Beta strandi33 – 353
Helixi39 – 4810
Helixi50 – 534
Helixi58 – 7417
Beta strandi82 – 843
Helixi85 – 10420
Helixi110 – 12112
Beta strandi125 – 1317
Helixi132 – 14211
Beta strandi143 – 1453
Helixi148 – 15710
Beta strandi164 – 1663
Helixi168 – 17912
Helixi184 – 1863
Turni187 – 1926

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EL4X-ray1.73A1-195[»]
1JF0X-ray1.82A1-195[»]
1JF2X-ray1.72A1-195[»]
1QV0X-ray1.10A1-195[»]
1QV1X-ray1.10A1-195[»]
1S36X-ray1.96A1-195[»]
1SL7X-ray2.20A1-195[»]
1SL9X-ray1.17A1-195[»]
2F8PX-ray1.93A1-195[»]
4MRXX-ray1.72A1-195[»]
4MRYX-ray1.30A1-195[»]
4N1FX-ray2.09A1-195[»]
4N1GX-ray1.50A/B1-195[»]
ProteinModelPortaliQ27709.
SMRiQ27709. Positions 2-195.

Miscellaneous databases

EvolutionaryTraceiQ27709.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 5236EF-hand 1
Add
BLAST
Domaini53 – 8836EF-hand 2
Add
BLAST
Domaini110 – 14536EF-hand 3
Add
BLAST
Domaini146 – 18136EF-hand 4
Add
BLAST

Sequence similaritiesi

Belongs to the aequorin family.
Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27709-1 [UniParc]FASTAAdd to Basket

« Hide

MSSKYAVKLK TDFDNPRWIK RHKHMFDFLD INGNGKITLD EIVSKASDDI    50
CAKLEATPEQ TKRHQVCVEA FFRGCGMEYG KEIAFPQFLD GWKQLATSEL 100
KKWARNEPTL IREWGDAVFD IFDKDGSGTI TLDEWKAYGK ISGISPSQED 150
CEATFRHCDL DNSGDLDVDE MTRQHLGFWY TLDPEADGLY GNGVP 195
Length:195
Mass (Da):22,226
Last modified:November 1, 1997 - v1
Checksum:i5D002270B73D3663
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07128 mRNA. Translation: AAA67708.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07128 mRNA. Translation: AAA67708.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EL4 X-ray 1.73 A 1-195 [» ]
1JF0 X-ray 1.82 A 1-195 [» ]
1JF2 X-ray 1.72 A 1-195 [» ]
1QV0 X-ray 1.10 A 1-195 [» ]
1QV1 X-ray 1.10 A 1-195 [» ]
1S36 X-ray 1.96 A 1-195 [» ]
1SL7 X-ray 2.20 A 1-195 [» ]
1SL9 X-ray 1.17 A 1-195 [» ]
2F8P X-ray 1.93 A 1-195 [» ]
4MRX X-ray 1.72 A 1-195 [» ]
4MRY X-ray 1.30 A 1-195 [» ]
4N1F X-ray 2.09 A 1-195 [» ]
4N1G X-ray 1.50 A/B 1-195 [» ]
ProteinModelPortali Q27709.
SMRi Q27709. Positions 2-195.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q27709.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 3 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima."
    Illarionov B.A., Bondar V.S., Illarionova V.A., Vysotski E.S.
    Gene 153:273-274(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure."
    Liu Z.J., Vysotski E.S., Chen C.J., Rose J.P., Lee J., Wang B.C.
    Protein Sci. 9:2085-2093(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS).
  3. "Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine."
    Liu Z.J., Vysotski E.S., Deng L., Lee J., Rose J., Wang B.C.
    Biochem. Biophys. Res. Commun. 311:433-439(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
  4. "Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence."
    Deng L., Markova S.V., Vysotski E.S., Liu Z.J., Lee J., Rose J., Wang B.C.
    J. Biol. Chem. 279:33647-33652(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS), MUTAGENESIS OF TRP-92.

Entry informationi

Entry nameiOBL_OBELO
AccessioniPrimary (citable) accession number: Q27709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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