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Q27709 (OBL_OBELO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Obelin

Short name=OBL
OrganismObelia longissima (Black sea hydrozoan) (Laomedea longissima)
Taxonomic identifier32570 [NCBI]
Taxonomic lineageEukaryotaMetazoaCnidariaHydrozoaHydroidaLeptomedusaeCampanulariidaeObelia

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ca2+-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO2 with the concomitant emission of light.

Sequence similarities

Belongs to the aequorin family.

Contains 4 EF-hand domains.

Ontologies

Keywords
   Biological processLuminescence
   DomainRepeat
   LigandCalcium
Metal-binding
   Molecular functionPhotoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 66 Potential
PRO_0000004136
Chain7 – 195189Obelin
PRO_0000004137

Regions

Domain17 – 5236EF-hand 1
Domain53 – 8836EF-hand 2
Domain110 – 14536EF-hand 3
Domain146 – 18136EF-hand 4
Calcium binding30 – 41121 Potential
Calcium binding123 – 134122 Potential
Calcium binding159 – 170123 Potential

Experimental info

Mutagenesis921W → F: Shifts luminescence to violet by adding a new band at 410 nm. Ref.4

Secondary structure

............................... 195
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q27709 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 5D002270B73D3663

FASTA19522,226
        10         20         30         40         50         60 
MSSKYAVKLK TDFDNPRWIK RHKHMFDFLD INGNGKITLD EIVSKASDDI CAKLEATPEQ 

        70         80         90        100        110        120 
TKRHQVCVEA FFRGCGMEYG KEIAFPQFLD GWKQLATSEL KKWARNEPTL IREWGDAVFD 

       130        140        150        160        170        180 
IFDKDGSGTI TLDEWKAYGK ISGISPSQED CEATFRHCDL DNSGDLDVDE MTRQHLGFWY 

       190 
TLDPEADGLY GNGVP 

« Hide

References

[1]"Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima."
Illarionov B.A., Bondar V.S., Illarionova V.A., Vysotski E.S.
Gene 153:273-274(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure."
Liu Z.J., Vysotski E.S., Chen C.J., Rose J.P., Lee J., Wang B.C.
Protein Sci. 9:2085-2093(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS).
[3]"Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine."
Liu Z.J., Vysotski E.S., Deng L., Lee J., Rose J., Wang B.C.
Biochem. Biophys. Res. Commun. 311:433-439(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
[4]"Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence."
Deng L., Markova S.V., Vysotski E.S., Liu Z.J., Lee J., Rose J., Wang B.C.
J. Biol. Chem. 279:33647-33652(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS), MUTAGENESIS OF TRP-92.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U07128 mRNA. Translation: AAA67708.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EL4X-ray1.73A1-195[»]
1JF0X-ray1.82A1-195[»]
1JF2X-ray1.72A1-195[»]
1QV0X-ray1.10A1-195[»]
1QV1X-ray1.10A1-195[»]
1S36X-ray1.96A1-195[»]
1SL7X-ray2.20A1-195[»]
1SL9X-ray1.17A1-195[»]
2F8PX-ray1.93A1-195[»]
4MRXX-ray1.72A1-195[»]
4MRYX-ray1.30A1-195[»]
4N1FX-ray2.09A1-195[»]
4N1GX-ray1.50A/B1-195[»]
ProteinModelPortalQ27709.
SMRQ27709. Positions 2-195.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 3 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ27709.

Entry information

Entry nameOBL_OBELO
AccessionPrimary (citable) accession number: Q27709
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references