ID   SYI_ANTLO               Reviewed;         628 AA.
AC   Q27707;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   13-SEP-2023, entry version 91.
DE   RecName: Full=Isoleucine--tRNA ligase;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
DE   Flags: Fragment;
OS   Antonospora locustae (Microsporidian parasite) (Nosema locustae).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Antonospora.
OX   NCBI_TaxID=278021;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7708661; DOI=10.1073/pnas.92.7.2441;
RA   Brown J.R., Doolittle W.F.;
RT   "Root of the universal tree of life based on ancient aminoacyl-tRNA
RT   synthetase gene duplications.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; L37097; AAC41564.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q27707; -.
DR   SMR; Q27707; -.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00392; ileS; 1.
DR   PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Nucleotide-binding;
KW   Protein biosynthesis.
FT   CHAIN           <1..>628
FT                   /note="Isoleucine--tRNA ligase"
FT                   /id="PRO_0000098601"
FT   MOTIF           505..509
FT                   /note="'KMSKS' region"
FT   BINDING         508
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         628
SQ   SEQUENCE   628 AA;  73774 MW;  8FCFCAD43362C35E CRC64;
     WDRHGVPVEY EIDKALGITG VKDIHDMGIR KYNKECRKIV LRYTKEWEAV VKRMGRWIDF
     RNGYRTMDRS FMESIWHIFK MLYEKGSVYR GHRVMPYSTA CSTPLSNFEA NQNYKDVSDP
     SILVAFPLRK PFKGYSLSLV AWTTTPWTLP SHMAILVNQS FIYAIFRLKS AFFIMQRDRV
     NVYFKDAVVI SEVKGHELLH LEHDQPFMYY NHYREKGFFR VYHADFVSEI DGTGVVHCSP
     GFGEDDYKAM TMPGLIKENE LLPSPLDENG RFNEEVPEYK GMHVKDADQS IIKDLGKKIL
     YEGKVYHRYP FCWRSDTPLI YKLVPNWFVR VKREIPRLLE SNSTINWIPE SIGEHKFKNW
     LSEARDWSIS RNRFWGTPIP LWHCDGKYIC IGSIEELSRL SGRKIDDIHR ENVDDVVICK
     DGEKYRRIEE VFDCWFESGC MPYAQRHWPF ECDNLCLPAD FVAEGVDQTR GWFYTMHVIS
     TVLLAKPRFR NCIVNGIVLA SDKKKMSKRL KNYPDPMDVI NRYSADSLRL YLISSPVVMA
     ENMCFSEEGV GEVLKTLLIP WYNCIYFYSE CPNAGDQEQT RMDDWILNTL NTFGNKVKRD
     MSKYMLQGVM GYATSFVNDL SNWYIRLN
//