Reviewed, UniProtKB/Swiss-Prot Q27707 (SYI_ANTLO)

Names and origin

Protein names	Recommended name:     Isoleucyl-tRNA synthetase     EC=6.1.1.5 Alternative name(s):     Isoleucine--tRNA ligase       Short name=IleRS
Organism	Antonospora locustae (Microsporidian parasite) (Nosema locustae)
Taxonomic identifier	278021 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Microsporidia › Antonospora

Protein attributes

Sequence length	628 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›628	›628	Isoleucyl-tRNA synthetase		PRO_0000098601
Regions
	Motif	505 – 509	5	"KMSKS" region
Sites
	Binding site	508	1	ATP By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	628	1

Sequences

	Sequence		Length	Mass (Da)
	Q27707-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8FCFCAD43362C35E	FASTA	628	73,774
	10 20 30 40 50 60 WDRHGVPVEY EIDKALGITG VKDIHDMGIR KYNKECRKIV LRYTKEWEAV VKRMGRWIDF 70 80 90 100 110 120 RNGYRTMDRS FMESIWHIFK MLYEKGSVYR GHRVMPYSTA CSTPLSNFEA NQNYKDVSDP 130 140 150 160 170 180 SILVAFPLRK PFKGYSLSLV AWTTTPWTLP SHMAILVNQS FIYAIFRLKS AFFIMQRDRV 190 200 210 220 230 240 NVYFKDAVVI SEVKGHELLH LEHDQPFMYY NHYREKGFFR VYHADFVSEI DGTGVVHCSP 250 260 270 280 290 300 GFGEDDYKAM TMPGLIKENE LLPSPLDENG RFNEEVPEYK GMHVKDADQS IIKDLGKKIL 310 320 330 340 350 360 YEGKVYHRYP FCWRSDTPLI YKLVPNWFVR VKREIPRLLE SNSTINWIPE SIGEHKFKNW 370 380 390 400 410 420 LSEARDWSIS RNRFWGTPIP LWHCDGKYIC IGSIEELSRL SGRKIDDIHR ENVDDVVICK 430 440 450 460 470 480 DGEKYRRIEE VFDCWFESGC MPYAQRHWPF ECDNLCLPAD FVAEGVDQTR GWFYTMHVIS 490 500 510 520 530 540 TVLLAKPRFR NCIVNGIVLA SDKKKMSKRL KNYPDPMDVI NRYSADSLRL YLISSPVVMA 550 560 570 580 590 600 ENMCFSEEGV GEVLKTLLIP WYNCIYFYSE CPNAGDQEQT RMDDWILNTL NTFGNKVKRD 610 620 MSKYMLQGVM GYATSFVNDL SNWYIRLN

References

[1]	"Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications." Brown J.R., Doolittle W.F. Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed: 7708661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37097 Genomic DNA. Translation: AAC41564.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.1.1.5. 288911.
Family and domain databases
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
PROSITE	PS00178. AA_TRNA_LIGASE_I. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_ANTLO

Accession

Primary (citable) accession number: Q27707

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)