Q27707 (SYI_ANTLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Isoleucine--tRNA ligase EC=6.1.1.5 Alternative name(s): Isoleucyl-tRNA synthetase Short name=IleRS
Organism	Antonospora locustae (Microsporidian parasite) (Nosema locustae)
Taxonomic identifier	278021 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Microsporidia incertae sedis › Antonospora

Protein attributes

Sequence length	628 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›628	›628	Isoleucine--tRNA ligase		PRO_0000098601
Regions
	Motif	505 – 509	5	"KMSKS" region
Sites
	Binding site	508	1	ATP By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	628	1

Sequences

	Sequence		Length	Mass (Da)
	Q27707 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8FCFCAD43362C35E	FASTA	628	73,774
	10 20 30 40 50 60 WDRHGVPVEY EIDKALGITG VKDIHDMGIR KYNKECRKIV LRYTKEWEAV VKRMGRWIDF 70 80 90 100 110 120 RNGYRTMDRS FMESIWHIFK MLYEKGSVYR GHRVMPYSTA CSTPLSNFEA NQNYKDVSDP 130 140 150 160 170 180 SILVAFPLRK PFKGYSLSLV AWTTTPWTLP SHMAILVNQS FIYAIFRLKS AFFIMQRDRV 190 200 210 220 230 240 NVYFKDAVVI SEVKGHELLH LEHDQPFMYY NHYREKGFFR VYHADFVSEI DGTGVVHCSP 250 260 270 280 290 300 GFGEDDYKAM TMPGLIKENE LLPSPLDENG RFNEEVPEYK GMHVKDADQS IIKDLGKKIL 310 320 330 340 350 360 YEGKVYHRYP FCWRSDTPLI YKLVPNWFVR VKREIPRLLE SNSTINWIPE SIGEHKFKNW 370 380 390 400 410 420 LSEARDWSIS RNRFWGTPIP LWHCDGKYIC IGSIEELSRL SGRKIDDIHR ENVDDVVICK 430 440 450 460 470 480 DGEKYRRIEE VFDCWFESGC MPYAQRHWPF ECDNLCLPAD FVAEGVDQTR GWFYTMHVIS 490 500 510 520 530 540 TVLLAKPRFR NCIVNGIVLA SDKKKMSKRL KNYPDPMDVI NRYSADSLRL YLISSPVVMA 550 560 570 580 590 600 ENMCFSEEGV GEVLKTLLIP WYNCIYFYSE CPNAGDQEQT RMDDWILNTL NTFGNKVKRD 610 620 MSKYMLQGVM GYATSFVNDL SNWYIRLN

References

[1]	"Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications." Brown J.R., Doolittle W.F. Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37097 Genomic DNA. Translation: AAC41564.1.
3D structure databases
ProteinModelPortal	Q27707.
ModBase	Search...
Proteomic databases
PRIDE	Q27707.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_ANTLO

Accession

Primary (citable) accession number: Q27707

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program