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Q27701

- NANL_MACDE

UniProt

Q27701 - NANL_MACDE

Protein

Anhydrosialidase

Gene
N/A
Organism
Macrobdella decora (North American leech)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei293 – 2931Substrate
    Active sitei318 – 3181Proton acceptor
    Active sitei595 – 5951
    Binding sitei611 – 6111Substrate
    Binding sitei673 – 6731Substrate
    Active sitei713 – 7131Nucleophile

    GO - Molecular functioni

    1. anhydrosialidase activity Source: UniProtKB-EC
    2. exo-alpha-sialidase activity Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Protein family/group databases

    CAZyiCBM40. Carbohydrate-Binding Module Family 40.
    GH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anhydrosialidase (EC:4.2.2.15)
    Alternative name(s):
    Anhydroneuraminidase
    Sialidase L
    OrganismiMacrobdella decora (North American leech)
    Taxonomic identifieri6405 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaAnnelidaClitellataHirudinidaHirudineaArhynchobdellidaHirudiniformesHirudinidaeMacrobdella

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 762735AnhydrosialidasePRO_0000012040Add
    BLAST

    Structurei

    Secondary structure

    1
    762
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi83 – 9412
    Turni96 – 983
    Helixi108 – 1114
    Beta strandi115 – 12511
    Beta strandi130 – 1389
    Beta strandi147 – 1548
    Beta strandi159 – 1657
    Turni167 – 1693
    Beta strandi171 – 1788
    Beta strandi182 – 1843
    Beta strandi191 – 1988
    Turni199 – 2024
    Beta strandi203 – 2086
    Beta strandi211 – 2177
    Helixi224 – 2263
    Beta strandi232 – 2409
    Beta strandi243 – 2464
    Beta strandi250 – 26011
    Helixi264 – 2718
    Beta strandi290 – 29910
    Beta strandi305 – 31410
    Beta strandi319 – 3213
    Beta strandi323 – 33210
    Beta strandi341 – 3444
    Helixi362 – 3643
    Beta strandi372 – 38110
    Turni382 – 3854
    Beta strandi386 – 3949
    Helixi400 – 4023
    Beta strandi408 – 4125
    Beta strandi415 – 4228
    Beta strandi432 – 4343
    Helixi436 – 4383
    Beta strandi439 – 4424
    Turni443 – 4464
    Beta strandi447 – 4537
    Beta strandi458 – 4614
    Beta strandi467 – 47610
    Beta strandi479 – 49012
    Beta strandi499 – 5024
    Beta strandi507 – 5159
    Helixi527 – 5293
    Beta strandi535 – 5395
    Beta strandi541 – 5433
    Turni551 – 5544
    Beta strandi556 – 56510
    Beta strandi567 – 5759
    Beta strandi581 – 5844
    Beta strandi596 – 6005
    Beta strandi606 – 6105
    Beta strandi613 – 6164
    Beta strandi618 – 6247
    Beta strandi648 – 6569
    Beta strandi659 – 6679
    Beta strandi670 – 6745
    Beta strandi676 – 68510
    Helixi690 – 6923
    Beta strandi693 – 70513
    Beta strandi714 – 7185
    Beta strandi720 – 7223
    Beta strandi724 – 7296
    Beta strandi745 – 7506
    Helixi752 – 7554

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SLIX-ray2.00A81-759[»]
    1SLLX-ray2.00A81-759[»]
    2SLIX-ray1.80A81-759[»]
    3SLIX-ray1.80A81-759[»]
    4SLIX-ray1.80A81-759[»]
    ProteinModelPortaliQ27701.
    SMRiQ27701. Positions 81-759.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ27701.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati328 – 33912BNR 1Add
    BLAST
    Repeati511 – 52212BNR 2Add
    BLAST
    Repeati571 – 58212BNR 3Add
    BLAST
    Repeati620 – 63112BNR 4Add
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 4 BNR repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.120.10.10. 2 hits.
    2.40.220.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR004124. Glyco_hydro_33_N.
    IPR001791. Laminin_G.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    IPR023364. Trans_sialidase_dom3.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    PfamiPF02973. Sialidase. 1 hit.
    [Graphical view]
    SMARTiSM00282. LamG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF50939. SSF50939. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27701-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRIGKKAMA IALVSAVMVT PLNVCATVEN QEQQQVTQGA EDIAVIDDAQ    50
    ETVAADEAQA DEAAAITVEG RETAEESSAS IPEGILMEKN NVDIAEGQGY 100
    SLDQEAGAKY VKAMTQGTII LSYKSTSENG IQSLFSVGNS TAGNQDRHFH 150
    IYITNSGGIG IELRNTDGVF NYTLDRPASV RALYKGERVF NTVALKADAA 200
    NKQCRLFANG ELLATLDKDA FKFISDITGV DNVTLGGTKR QGKIAYPFGG 250
    TIGDIKVYSN ALSDEELIQA TGVTTYGENI FYAGDVTESN YFRIPSLLTL 300
    STGTVISAAD ARYGGTHDSK SKINIAFAKS TDGGNTWSEP TLPLKFDDYI 350
    AKNIDWPRDS VGKNVQIQGS ASYIDPVLLE DKLTKRIFLF ADLMPAGIGS 400
    SNASVGSGFK EVNGKKYLKL RWHKDAGRAY DYTIREKGVI YNDATNQPTE 450
    FRVDGEYNLY QHDTNLTCKQ YDYNFSGNNL IESKTDVDVN MNIFYKNSVF 500
    KAFPTNYLAM RYSDDEGASW SDLDIVSSFK PEVSKFLVVG PGIGKQISTG 550
    ENAGRLLVPL YSKSSAELGF MYSDDHGDNW TYVEADNLTG GATAEAQIVE 600
    MPDGSLKTYL RTGSNCIAEV TSIDGGETWS DRVPLQGIST TSYGTQLSVI 650
    NYSQPIDGKP AIILSSPNAT NGRKNGKIWI GLVNDTGNTG IDKYSVEWKY 700
    SYAVDTPQMG YSYSCLAELP DGQVGLLYEK YDSWSRNELH LKDILKFEKY 750
    SISELTGQAS GN 762
    Length:762
    Mass (Da):82,982
    Last modified:November 1, 1996 - v1
    Checksum:iC0C547C7A8632B37
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46666 mRNA. Translation: AAC47263.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46666 mRNA. Translation: AAC47263.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SLI X-ray 2.00 A 81-759 [» ]
    1SLL X-ray 2.00 A 81-759 [» ]
    2SLI X-ray 1.80 A 81-759 [» ]
    3SLI X-ray 1.80 A 81-759 [» ]
    4SLI X-ray 1.80 A 81-759 [» ]
    ProteinModelPortali Q27701.
    SMRi Q27701. Positions 81-759.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM40. Carbohydrate-Binding Module Family 40.
    GH33. Glycoside Hydrolase Family 33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q27701.

    Family and domain databases

    Gene3Di 2.120.10.10. 2 hits.
    2.40.220.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR004124. Glyco_hydro_33_N.
    IPR001791. Laminin_G.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    IPR023364. Trans_sialidase_dom3.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    Pfami PF02973. Sialidase. 1 hit.
    [Graphical view ]
    SMARTi SM00282. LamG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF50939. SSF50939. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of sialidase L, a NeuAc-alpha2-->3Gal-specific sialidase from the leech, Macrobdella decora."
      Chou M.-Y., Li S.-C., Li Y.-T.
      J. Biol. Chem. 271:19219-19224(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Purification and characterization of sialidase L, a NeuAc-alpha 2-->3Gal-specific sialidase."
      Chou M.-Y., Li S.-C., Kiso M., Hasegawa A., Li Y.-T.
      J. Biol. Chem. 269:18821-18826(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 89-104; 330-338; 346-352; 387-406; 429-435; 438-469 AND 512-530, CHARACTERIZATION.
    3. "The crystal structure of an intramolecular trans-sialidase with a NeuAc-alpha2-->3Gal specificity."
      Luo Y., Li S.-C., Chou M.-Y., Li Y.-T., Luo M.
      Structure 6:521-530(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 81-759.
    4. "The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism."
      Luo Y., Li S.-C., Li Y.-T., Luo M.
      J. Mol. Biol. 285:323-332(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 81-759.

    Entry informationi

    Entry nameiNANL_MACDE
    AccessioniPrimary (citable) accession number: Q27701
    Secondary accession number(s): Q9TWN0, Q9TWN1, Q9TWN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3