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Protein

Cytochrome P450 6d1

Gene

CYP6D1

Organism
Musca domestica (House fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metabolizes pyrethroid insecticides and other xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi461Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 6d1 (EC:1.14.-.-)
Alternative name(s):
CYPVID1
Cytochrome P450 Ipr
Pyrethroid resistance cytochrome P450
Gene namesi
Name:CYP6D1
OrganismiMusca domestica (House fly)
Taxonomic identifieri7370 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaMuscoideaMuscidaeMusca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000518781 – 516Cytochrome P450 6d1Add BLAST516

Expressioni

Developmental stagei

Found only in adults and late pupae.

Inductioni

By phenobarbital in susceptible strains (3-fold in CS), but not in the LPR strain.

Structurei

3D structure databases

ProteinModelPortaliQ27698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLLLLIVV TTLYIFAKLH YTKWERLGFE SDKATIPLGS MAKVFHKERP
60 70 80 90 100
FGLVMSDIYD KCHEKVVGIY LFFKPALLVR DAELARQILT TDFNSFHDRG
110 120 130 140 150
LYVDEKNDPM SANLFVMEGQ SWRTLRMKLA PSFSSGKLKG MFETVDDVAA
160 170 180 190 200
KLLNHLNERL KDGQSHVLEI KSILTTYAVD IIGSVIFGLE IDSFTHPDNE
210 220 230 240 250
FRVLSDRLFN PKKSTMLQRF RNLSNFICPP LAKLLSRLGA KDPITYRLRD
260 270 280 290 300
IVKRTIEFRE EKGVVRKDLL QLFIQLRNTG KISDDNDKLW HDVESTAENL
310 320 330 340 350
KAMSIDMIAS NSFLFYIAGS ETTAATTSFT IYELAMYPEI LKKAQSEVDE
360 370 380 390 400
CLQRHGLKPQ GRLTYEAIQD MKYLDLCVME TTRKYPGLPF LNRKCTQDFQ
410 420 430 440 450
VPDTKLTIPK ETGIIISLLG IHRDPQYFPQ PEDYRPERFA DESKDYDPAA
460 470 480 490 500
YMPFGEGPRH CIAQRMGVMN SKVALAKILA NFNIQPMPRQ EVEFKFHSAP
510
VLVPVNGLNV GLSKRW
Length:516
Mass (Da):59,173
Last modified:November 1, 1996 - v1
Checksum:i654E32FB34847B16
GO

Polymorphismi

Alleles from the pyrethroid-susceptible strains CS (CYP6D1v2) (shown here), aabys (CYP6D1v3), ISK (CYP6D1v4), OCR (CYP6D1v5) and Edinburgh, and one allele from the pyrethroid-resistant strain LPR (CYP6D1v1) are described here.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2Missing in allele CYP6D1v3. 2 Publications1
Natural varianti55M → L in allele CYP6D1v3 and allele CYP6D1v5. 3 Publications1
Natural varianti150A → D in allele CYP6D1v1. 4 Publications1
Natural varianti153L → I in allele CYP6D1v1. 4 Publications1
Natural varianti165S → T in allele CYP6D1v1. 4 Publications1
Natural varianti182I → N in allele CYP6D1v5. 1 Publication1
Natural varianti218Q → E in allele CYP6D1v5 and allele CYP6D1v1. 4 Publications1
Natural varianti220F → I in allele CYP6D1v1. 4 Publications1
Natural varianti225 – 227NFI → TFM in allele CYP6D1v5 and allele CYP6D1v1. 3
Natural varianti262K → T in allele CYP6D1v5. 1 Publication1
Natural varianti266R → P in allele CYP6D1v3. 2 Publications1
Natural varianti447D → N in allele CYP6D1v5. 1 Publication1
Natural varianti469M → I in allele CYP6D1v4, allele CYP6D1v5 and allele CYP6D1v1. 4 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22367 mRNA. Translation: AAC46931.1.
U22366 mRNA. Translation: AAC46930.1.
AF064795 Genomic DNA. Translation: AAC99341.1.
AF081288 mRNA. Translation: AAD32478.1.
U22362 mRNA. Translation: AAC46932.1.
AF200191 Genomic DNA. Translation: AAG28564.1.
U15168 mRNA. Translation: AAA81513.1.
AF064794 Genomic DNA. Translation: AAC99340.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22367 mRNA. Translation: AAC46931.1.
U22366 mRNA. Translation: AAC46930.1.
AF064795 Genomic DNA. Translation: AAC99341.1.
AF081288 mRNA. Translation: AAD32478.1.
U22362 mRNA. Translation: AAC46932.1.
AF200191 Genomic DNA. Translation: AAG28564.1.
U15168 mRNA. Translation: AAA81513.1.
AF064794 Genomic DNA. Translation: AAC99340.1.

3D structure databases

ProteinModelPortaliQ27698.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP6D1_MUSDO
AccessioniPrimary (citable) accession number: Q27698
Secondary accession number(s): Q27697
, Q27699, Q27700, Q9GU29, Q9XYB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Expressed at higher levels in LPR compared to susceptible flies, it is responsible for monooxygenase-mediated pyrethroid resistance.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.