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Q27684 (PGKB_LEIME) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, cytosolic

EC=2.7.2.3
Alternative name(s):
Phosphoglycerate kinase B
Gene names
Name:PGKB
OrganismLeishmania mexicana
Taxonomic identifier5665 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Domain

This cytosolic PGK lacks a C-terminal extension of 38 AA which is present in the glycosomal isoenzyme. HAMAP-Rule MF_00145

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Phosphoglycerate kinase, cytosolic HAMAP-Rule MF_00145
PRO_0000145855

Regions

Nucleotide binding372 – 3754ATP By similarity
Region24 – 263Substrate binding By similarity
Region62 – 654Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1321Substrate By similarity
Binding site1691Substrate By similarity
Binding site2201ATP By similarity
Binding site3421ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27684 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F79168E2C90C166D

FASTA41744,968
        10         20         30         40         50         60 
MSLVLKKSID DVALKDKKVL IRVDFNVPVK NGEITNDFRI RSALPTIQKV LKEGGSCILM 

        70         80         90        100        110        120 
SHLGRPKGAR MSDPKPEKGV RGYEEAATLR PVAARLSELL EEKVAKAPDC LNASIYVSKL 

       130        140        150        160        170        180 
KRGDVLLLEN VRFYTEEGSK KEEEADAMAK VLASYADLYV SDAFGTAHRD SATMTGIPKV 

       190        200        210        220        230        240 
LGSGYAGYLM EKEINYFSRV LNNPPRPLVA IVGGAKVSDK IELLDNMLGR INYLVIGGAM 

       250        260        270        280        290        300 
AYTFQKAQGR KIGISMCEED KLDLAKSLLK KAQERGVQVL LPVDHVCNKE FKAVDSPLVT 

       310        320        330        340        350        360 
EDVDVPDGYM ALDIGPKTIH MYEEVIGRCK SAIWNGPMGV FEMPCISKGT FAVAKAMGTG 

       370        380        390        400        410 
TQKDGLLSII GGGDTASAAE LSARAKNMSH VSTGGGASLE LLEGKTLPGV AILTDKQ 

« Hide

References

[1]"Organization, sequence and stage-specific expression of the phosphoglycerate kinase genes of Leishmania mexicana mexicana."
Adje C.A., Opperdoes F.R., Michels P.A.M.
Mol. Biochem. Parasitol. 90:155-168(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MHOM/BZ/84/BEL46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98486 Genomic DNA. Translation: CAA67112.1.

3D structure databases

ProteinModelPortalQ27684.
SMRQ27684. Positions 6-416.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKB_LEIME
AccessionPrimary (citable) accession number: Q27684
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways