Q27677 (ACES_LEPDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Acetylcholinesterase Short name=AChE EC=3.1.1.7
Organism	Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
Taxonomic identifier	7539 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Doryphorini › Leptinotarsa

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Rapidly hydrolyzes choline released into the synapse.
Catalytic activity	Acetylcholine + H2O = choline + acetate.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Cell junction › synapse By similarity. Cell membrane; Lipid-anchor › GPI-anchor By similarity. Note: Attached to the membrane of the neuronal cholinergic synapses by a GPI-anchor By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Synapse
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological_process	acetylcholine catabolic process in synaptic cleft Inferred from electronic annotation. Source: InterPro
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW cell junction Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholinesterase activity Inferred from electronic annotation. Source: EC carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 38	38	Potential
	Chain	39 – 605	567	Acetylcholinesterase		PRO_0000008607
	Propeptide	606 – 629	24	Removed in mature form Potential		PRO_0000008608
Sites
	Active site	253	1	Acyl-ester intermediate By similarity
	Active site	382	1	Charge relay system By similarity
	Active site	496	1	Charge relay system By similarity
Amino acid modifications
	Lipidation	605	1	GPI-anchor amidated serine Potential
	Glycosylation	125	1	N-linked (GlcNAc...) By similarity
	Glycosylation	308	1	N-linked (GlcNAc...) By similarity
	Glycosylation	418	1	N-linked (GlcNAc...) By similarity
	Glycosylation	509	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	103 ↔ 130		By similarity
	Disulfide bond	307 ↔ 322		By similarity
	Disulfide bond	458 ↔ 574		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27677 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 06556F833EB16C72	FASTA	629	71,142
	10 20 30 40 50 60 MGQLSILCLF VTVCASVCGY SWPSDETTTK PSQFKDFHTD PLVVETTSGL VRGYSKTVLG 70 80 90 100 110 120 REVHVFTGIP FAKPPIEQLR FKKPVPIDPW HGILDATKQP NSCFQERYEY FPGFEGEEMW 130 140 150 160 170 180 NPNTNISEDC LYLNIWVPQR LRIRHHADKP TIDRPKVPVL IWIYGGGYMS GTATLDVYDA 190 200 210 220 230 240 DIIAATSDVI VASMQYRLGS FGFLYLNRYF PRGSDETPGN MGLWDQILAI RWIKDNAAAF 250 260 270 280 290 300 GGDPDLITLF GESAGGGSIS IHLISPVTKG LVRRGIMQSG TMNAPWSYMS GERAEQIGKI 310 320 330 340 350 360 LIQDCGCNVS LLENSPRKVM DCMRAVDAKT ISLQQWNSYS GILGFPSTPT IEGVLLPKHP 370 380 390 400 410 420 MDMLAEGDYE DMEILLGSNH DEGTYFLLYD FIDFFEKDGP SFLQREKYHD IIDTIFKNMS 430 440 450 460 470 480 RLERDAIVFQ YTNWEHVHDG YLNQKMIGDV VGDYFFVCPT NNFAEVAADR GMKVFYYYFT 490 500 510 520 530 540 HRTSTSLWGE WMGVIHGDEV EYVFGHPLNM SLQFNSRERE LSLKIMQAFA RFATTGKPVT 550 560 570 580 590 600 DDVNWPLYTK DQPQYFIFNA DKNGIGKGPR ATACAFWNDF LPKLRDNSGS EEAPCVNTYL 610 620 SKIRSSSNEL LPPSTSLVLI WIMTLLNAL

References

[1]

"Cloning and sequencing of a cDNA encoding acetylcholinesterase in Colorado potato beetle, Leptinotarsa decemlineata (Say)." Zhu K.Y., Clark J.M. Insect Biochem. Mol. Biol. 25:1129-1138(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SS. Tissue: Larva and Pupae.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L41180 mRNA. Translation: AAB00466.1.
3D structure databases
ProteinModelPortal	Q27677.
SMR	Q27677. Positions 40-585.
ModBase	Search...
Protein family/group databases
MEROPS	S09.979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001445. Acylcholinesterase_insect. IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00880. ACHEINSECT. PR00878. CHOLNESTRASE.
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACES_LEPDE

Accession

Primary (citable) accession number: Q27677

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)