SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q27677

- ACES_LEPDE

UniProt

Q27677 - ACES_LEPDE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Acetylcholinesterase
Gene
N/A
Organism
Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Rapidly hydrolyzes choline released into the synapse.

Catalytic activityi

Acetylcholine + H2O = choline + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei253 – 2531Acyl-ester intermediate By similarity
Active sitei382 – 3821Charge relay system By similarity
Active sitei496 – 4961Charge relay system By similarity

GO - Molecular functioni

  1. acetylcholinesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. acetylcholine catabolic process in synaptic cleft Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Neurotransmitter degradation

Protein family/group databases

MEROPSiS09.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
OrganismiLeptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
Taxonomic identifieri7539 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeDoryphoriniLeptinotarsa

Subcellular locationi

Cell junctionsynapse By similarity. Cell membrane; Lipid-anchorGPI-anchor By similarity
Note: Attached to the membrane of the neuronal cholinergic synapses by a GPI-anchor By similarity.

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell junction Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
  4. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838 Reviewed prediction
Add
BLAST
Chaini39 – 605567Acetylcholinesterase
PRO_0000008607Add
BLAST
Propeptidei606 – 62924Removed in mature form Reviewed prediction
PRO_0000008608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi103 ↔ 130 By similarity
Glycosylationi125 – 1251N-linked (GlcNAc...) By similarity
Disulfide bondi307 ↔ 322 By similarity
Glycosylationi308 – 3081N-linked (GlcNAc...) By similarity
Glycosylationi418 – 4181N-linked (GlcNAc...) By similarity
Disulfide bondi458 ↔ 574 By similarity
Glycosylationi509 – 5091N-linked (GlcNAc...) By similarity
Lipidationi605 – 6051GPI-anchor amidated serine Reviewed prediction

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ27677.
SMRiQ27677. Positions 40-585.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001445. Acylcholinesterase_insect.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00880. ACHEINSECT.
PR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27677-1 [UniParc]FASTAAdd to Basket

« Hide

MGQLSILCLF VTVCASVCGY SWPSDETTTK PSQFKDFHTD PLVVETTSGL    50
VRGYSKTVLG REVHVFTGIP FAKPPIEQLR FKKPVPIDPW HGILDATKQP 100
NSCFQERYEY FPGFEGEEMW NPNTNISEDC LYLNIWVPQR LRIRHHADKP 150
TIDRPKVPVL IWIYGGGYMS GTATLDVYDA DIIAATSDVI VASMQYRLGS 200
FGFLYLNRYF PRGSDETPGN MGLWDQILAI RWIKDNAAAF GGDPDLITLF 250
GESAGGGSIS IHLISPVTKG LVRRGIMQSG TMNAPWSYMS GERAEQIGKI 300
LIQDCGCNVS LLENSPRKVM DCMRAVDAKT ISLQQWNSYS GILGFPSTPT 350
IEGVLLPKHP MDMLAEGDYE DMEILLGSNH DEGTYFLLYD FIDFFEKDGP 400
SFLQREKYHD IIDTIFKNMS RLERDAIVFQ YTNWEHVHDG YLNQKMIGDV 450
VGDYFFVCPT NNFAEVAADR GMKVFYYYFT HRTSTSLWGE WMGVIHGDEV 500
EYVFGHPLNM SLQFNSRERE LSLKIMQAFA RFATTGKPVT DDVNWPLYTK 550
DQPQYFIFNA DKNGIGKGPR ATACAFWNDF LPKLRDNSGS EEAPCVNTYL 600
SKIRSSSNEL LPPSTSLVLI WIMTLLNAL 629
Length:629
Mass (Da):71,142
Last modified:November 1, 1996 - v1
Checksum:i06556F833EB16C72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L41180 mRNA. Translation: AAB00466.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L41180 mRNA. Translation: AAB00466.1 .

3D structure databases

ProteinModelPortali Q27677.
SMRi Q27677. Positions 40-585.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S09.979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR001445. Acylcholinesterase_insect.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view ]
Pfami PF00135. COesterase. 1 hit.
[Graphical view ]
PRINTSi PR00880. ACHEINSECT.
PR00878. CHOLNESTRASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a cDNA encoding acetylcholinesterase in Colorado potato beetle, Leptinotarsa decemlineata (Say)."
    Zhu K.Y., Clark J.M.
    Insect Biochem. Mol. Biol. 25:1129-1138(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SS.
    Tissue: Larva and Pupae.

Entry informationi

Entry nameiACES_LEPDE
AccessioniPrimary (citable) accession number: Q27677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi