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Protein

Acetylcholinesterase

Gene
N/A
Organism
Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Rapidly hydrolyzes choline released into the synapse.

Catalytic activityi

Acetylcholine + H2O = choline + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei253Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei382Charge relay systemBy similarity1
Active sitei496Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Neurotransmitter degradation

Protein family/group databases

ESTHERilepde-ACHE. AChE.
MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
OrganismiLeptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
Taxonomic identifieri7539 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeDoryphoriniLeptinotarsa

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2366490.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000000860739 – 605AcetylcholinesteraseAdd BLAST567
PropeptideiPRO_0000008608606 – 629Removed in mature formSequence analysisAdd BLAST24

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi103 ↔ 130By similarity
Glycosylationi125N-linked (GlcNAc...)By similarity1
Disulfide bondi307 ↔ 322By similarity
Glycosylationi308N-linked (GlcNAc...)By similarity1
Glycosylationi418N-linked (GlcNAc...)By similarity1
Disulfide bondi458 ↔ 574By similarity
Glycosylationi509N-linked (GlcNAc...)By similarity1
Lipidationi605GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ27677.
SMRiQ27677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001445. Acylcholinesterase_insect.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00880. ACHEINSECT.
PR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQLSILCLF VTVCASVCGY SWPSDETTTK PSQFKDFHTD PLVVETTSGL
60 70 80 90 100
VRGYSKTVLG REVHVFTGIP FAKPPIEQLR FKKPVPIDPW HGILDATKQP
110 120 130 140 150
NSCFQERYEY FPGFEGEEMW NPNTNISEDC LYLNIWVPQR LRIRHHADKP
160 170 180 190 200
TIDRPKVPVL IWIYGGGYMS GTATLDVYDA DIIAATSDVI VASMQYRLGS
210 220 230 240 250
FGFLYLNRYF PRGSDETPGN MGLWDQILAI RWIKDNAAAF GGDPDLITLF
260 270 280 290 300
GESAGGGSIS IHLISPVTKG LVRRGIMQSG TMNAPWSYMS GERAEQIGKI
310 320 330 340 350
LIQDCGCNVS LLENSPRKVM DCMRAVDAKT ISLQQWNSYS GILGFPSTPT
360 370 380 390 400
IEGVLLPKHP MDMLAEGDYE DMEILLGSNH DEGTYFLLYD FIDFFEKDGP
410 420 430 440 450
SFLQREKYHD IIDTIFKNMS RLERDAIVFQ YTNWEHVHDG YLNQKMIGDV
460 470 480 490 500
VGDYFFVCPT NNFAEVAADR GMKVFYYYFT HRTSTSLWGE WMGVIHGDEV
510 520 530 540 550
EYVFGHPLNM SLQFNSRERE LSLKIMQAFA RFATTGKPVT DDVNWPLYTK
560 570 580 590 600
DQPQYFIFNA DKNGIGKGPR ATACAFWNDF LPKLRDNSGS EEAPCVNTYL
610 620
SKIRSSSNEL LPPSTSLVLI WIMTLLNAL
Length:629
Mass (Da):71,142
Last modified:November 1, 1996 - v1
Checksum:i06556F833EB16C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41180 mRNA. Translation: AAB00466.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41180 mRNA. Translation: AAB00466.1.

3D structure databases

ProteinModelPortaliQ27677.
SMRiQ27677.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL2366490.

Protein family/group databases

ESTHERilepde-ACHE. AChE.
MEROPSiS09.980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001445. Acylcholinesterase_insect.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00880. ACHEINSECT.
PR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACES_LEPDE
AccessioniPrimary (citable) accession number: Q27677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.