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Q27677

- ACES_LEPDE

UniProt

Q27677 - ACES_LEPDE

Protein

Acetylcholinesterase

Gene
N/A
Organism
Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Rapidly hydrolyzes choline released into the synapse.

    Catalytic activityi

    Acetylcholine + H2O = choline + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei253 – 2531Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei382 – 3821Charge relay systemBy similarity
    Active sitei496 – 4961Charge relay systemBy similarity

    GO - Molecular functioni

    1. acetylcholinesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. acetylcholine catabolic process in synaptic cleft Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Neurotransmitter degradation

    Protein family/group databases

    MEROPSiS09.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetylcholinesterase (EC:3.1.1.7)
    Short name:
    AChE
    OrganismiLeptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata)
    Taxonomic identifieri7539 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeDoryphoriniLeptinotarsa

    Subcellular locationi

    Cell junctionsynapse By similarity. Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity
    Note: Attached to the membrane of the neuronal cholinergic synapses by a GPI-anchor.By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cell junction Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell
    4. synapse Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3838Sequence AnalysisAdd
    BLAST
    Chaini39 – 605567AcetylcholinesterasePRO_0000008607Add
    BLAST
    Propeptidei606 – 62924Removed in mature formSequence AnalysisPRO_0000008608Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi103 ↔ 130By similarity
    Glycosylationi125 – 1251N-linked (GlcNAc...)By similarity
    Disulfide bondi307 ↔ 322By similarity
    Glycosylationi308 – 3081N-linked (GlcNAc...)By similarity
    Glycosylationi418 – 4181N-linked (GlcNAc...)By similarity
    Disulfide bondi458 ↔ 574By similarity
    Glycosylationi509 – 5091N-linked (GlcNAc...)By similarity
    Lipidationi605 – 6051GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ27677.
    SMRiQ27677. Positions 40-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001445. Acylcholinesterase_insect.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR00880. ACHEINSECT.
    PR00878. CHOLNESTRASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27677-1 [UniParc]FASTAAdd to Basket

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    MGQLSILCLF VTVCASVCGY SWPSDETTTK PSQFKDFHTD PLVVETTSGL    50
    VRGYSKTVLG REVHVFTGIP FAKPPIEQLR FKKPVPIDPW HGILDATKQP 100
    NSCFQERYEY FPGFEGEEMW NPNTNISEDC LYLNIWVPQR LRIRHHADKP 150
    TIDRPKVPVL IWIYGGGYMS GTATLDVYDA DIIAATSDVI VASMQYRLGS 200
    FGFLYLNRYF PRGSDETPGN MGLWDQILAI RWIKDNAAAF GGDPDLITLF 250
    GESAGGGSIS IHLISPVTKG LVRRGIMQSG TMNAPWSYMS GERAEQIGKI 300
    LIQDCGCNVS LLENSPRKVM DCMRAVDAKT ISLQQWNSYS GILGFPSTPT 350
    IEGVLLPKHP MDMLAEGDYE DMEILLGSNH DEGTYFLLYD FIDFFEKDGP 400
    SFLQREKYHD IIDTIFKNMS RLERDAIVFQ YTNWEHVHDG YLNQKMIGDV 450
    VGDYFFVCPT NNFAEVAADR GMKVFYYYFT HRTSTSLWGE WMGVIHGDEV 500
    EYVFGHPLNM SLQFNSRERE LSLKIMQAFA RFATTGKPVT DDVNWPLYTK 550
    DQPQYFIFNA DKNGIGKGPR ATACAFWNDF LPKLRDNSGS EEAPCVNTYL 600
    SKIRSSSNEL LPPSTSLVLI WIMTLLNAL 629
    Length:629
    Mass (Da):71,142
    Last modified:November 1, 1996 - v1
    Checksum:i06556F833EB16C72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L41180 mRNA. Translation: AAB00466.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L41180 mRNA. Translation: AAB00466.1 .

    3D structure databases

    ProteinModelPortali Q27677.
    SMRi Q27677. Positions 40-585.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S09.979.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR001445. Acylcholinesterase_insect.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view ]
    Pfami PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR00880. ACHEINSECT.
    PR00878. CHOLNESTRASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a cDNA encoding acetylcholinesterase in Colorado potato beetle, Leptinotarsa decemlineata (Say)."
      Zhu K.Y., Clark J.M.
      Insect Biochem. Mol. Biol. 25:1129-1138(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: SS.
      Tissue: Larva and Pupae.

    Entry informationi

    Entry nameiACES_LEPDE
    AccessioniPrimary (citable) accession number: Q27677
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3