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Q27665

- PFKA_HAECO

UniProt

Q27665 - PFKA_HAECO

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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Haemonchus contortus (Barber pole worm)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.1 PublicationUniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP; via amide nitrogenUniRule annotation
Metal bindingi135 – 1351Magnesium; catalyticUniRule annotation
Active sitei182 – 1821Proton acceptorUniRule annotation
Binding sitei217 – 2171Substrate; shared with dimeric partnerUniRule annotation
Binding sitei280 – 2801SubstrateUniRule annotation
Binding sitei307 – 3071Substrate; shared with dimeric partnerUniRule annotation
Binding sitei489 – 4891Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei585 – 5851Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei647 – 6471Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei673 – 6731Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei753 – 7531Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1052ATPUniRule annotation
Nucleotide bindingi134 – 1374ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFK
OrganismiHaemonchus contortus (Barber pole worm)
Taxonomic identifieri6289 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 789789ATP-dependent 6-phosphofructokinasePRO_0000112032Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ27665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 404404N-terminal catalytic PFK domain 1Add
BLAST
Regioni180 – 1823Substrate bindingUniRule annotation
Regioni224 – 2263Substrate bindingUniRule annotation
Regioni313 – 3164Substrate bindingUniRule annotation
Regioni405 – 41915Interdomain linkerAdd
BLAST
Regioni420 – 789370C-terminal regulatory PFK domain 2Add
BLAST
Regioni547 – 5515Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni592 – 5943Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni679 – 6824Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27665-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLKRNIRRL DSIVPTAGRE GSDIQFNLYK GRGVAVFTSG GDSQGMNGAV
60 70 80 90 100
HSVVRMGIYL GCKVCFINEG YQGMVDGGDN IVEASWNSGS DIIQKGGTII
110 120 130 140 150
GSARCTDLRQ REGRMKAAYN LIEKGITNLV VIGGDGSLIG ANQFRKDWPG
160 170 180 190 200
LVKELVDTKK ITPEAAKSYP NIQIVGLVGS IDNDFCGTDM TIGTDSALQR
210 220 230 240 250
IIESIDAVVA TAQSHQRAFV VEVMGRHCGY LALVAALACE ADFCFIPEWP
260 270 280 290 300
PPVNWREILC KKLQEMRAEG QRLNIIVVAE DDDRDGTPIS SDLVKDVVAK
310 320 330 340 350
TLKYDTRVTV LGHVQRGGSP SAFDRLLGCR MGAEAVLALM EMNEESEPCV
360 370 380 390 400
ISIMVTRWYV PLMQCVERTK AVQKAMSEKD WELAVKLRGR SFQRNLETYK
410 420 430 440 450
LLTKLRTVEK DNLSGGQNFN VAVMNVGAPA GGMNAAVRSF VRMAIVPSLY
460 470 480 490 500
SLRYEDSFEG LANGAFKKFQ WGDVTNWVMH GGSFLGTQKQ LPNEKNVPLI
510 520 530 540 550
AEQLRKHNIQ ALLLVGGFEA YHSTLILSKN REKYPEFCIP LCVIPCTISN
560 570 580 590 600
NVPGTSISLG SDTAINEICT MIDKIKQSAT GTKRRVFIIE TMGGYCGYLA
610 620 630 640 650
TLSALASGAD NAYIFEEKFT VEISLRRGSH RCKMAQGVQR YLIVRNEYAN
660 670 680 690 700
KNFTTEFVKQ LFAEEGKGEF STRINILGHA QQGGSPTPFD RNMGTKLAAR
710 720 730 740 750
ALEYIITQIK ESMVNGVVST KSPERATLLG LTGRRVVFTP VEELAAETDF
760 770 780
DKRLPCDQWW LKLRPLLRIL AKHTSIYHTE AMEDTEDYD
Length:789
Mass (Da):87,184
Last modified:November 1, 1996 - v1
Checksum:i1245288AE2289DCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59805 mRNA. Translation: AAA29181.1.
PIRiA45617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59805 mRNA. Translation: AAA29181.1 .
PIRi A45617.

3D structure databases

ProteinModelPortali Q27665.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.

Entry informationi

Entry nameiPFKA_HAECO
AccessioniPrimary (citable) accession number: Q27665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3