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Q27665

- PFKA_HAECO

UniProt

Q27665 - PFKA_HAECO

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Protein

ATP-dependent 6-phosphofructokinase

Gene
PFK
Organism
Haemonchus contortus (Barber pole worm)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP; via amide nitrogen By similarity
Metal bindingi135 – 1351Magnesium; catalytic By similarity
Active sitei182 – 1821Proton acceptor By similarity
Binding sitei217 – 2171Substrate; shared with dimeric partner By similarity
Binding sitei280 – 2801Substrate By similarity
Binding sitei307 – 3071Substrate; shared with dimeric partner By similarity
Binding sitei489 – 4891Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei585 – 5851Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei647 – 6471Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei673 – 6731Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei753 – 7531Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1052ATP By similarity
Nucleotide bindingi134 – 1374ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:PFK
OrganismiHaemonchus contortus (Barber pole worm)
Taxonomic identifieri6289 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 789789ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000112032Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ27665.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 404404N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni180 – 1823Substrate binding By similarity
Regioni224 – 2263Substrate binding By similarity
Regioni313 – 3164Substrate binding By similarity
Regioni405 – 41915Interdomain linkerUniRule annotationAdd
BLAST
Regioni420 – 789370C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni547 – 5515Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni592 – 5943Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni679 – 6824Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27665-1 [UniParc]FASTAAdd to Basket

« Hide

MSLKRNIRRL DSIVPTAGRE GSDIQFNLYK GRGVAVFTSG GDSQGMNGAV    50
HSVVRMGIYL GCKVCFINEG YQGMVDGGDN IVEASWNSGS DIIQKGGTII 100
GSARCTDLRQ REGRMKAAYN LIEKGITNLV VIGGDGSLIG ANQFRKDWPG 150
LVKELVDTKK ITPEAAKSYP NIQIVGLVGS IDNDFCGTDM TIGTDSALQR 200
IIESIDAVVA TAQSHQRAFV VEVMGRHCGY LALVAALACE ADFCFIPEWP 250
PPVNWREILC KKLQEMRAEG QRLNIIVVAE DDDRDGTPIS SDLVKDVVAK 300
TLKYDTRVTV LGHVQRGGSP SAFDRLLGCR MGAEAVLALM EMNEESEPCV 350
ISIMVTRWYV PLMQCVERTK AVQKAMSEKD WELAVKLRGR SFQRNLETYK 400
LLTKLRTVEK DNLSGGQNFN VAVMNVGAPA GGMNAAVRSF VRMAIVPSLY 450
SLRYEDSFEG LANGAFKKFQ WGDVTNWVMH GGSFLGTQKQ LPNEKNVPLI 500
AEQLRKHNIQ ALLLVGGFEA YHSTLILSKN REKYPEFCIP LCVIPCTISN 550
NVPGTSISLG SDTAINEICT MIDKIKQSAT GTKRRVFIIE TMGGYCGYLA 600
TLSALASGAD NAYIFEEKFT VEISLRRGSH RCKMAQGVQR YLIVRNEYAN 650
KNFTTEFVKQ LFAEEGKGEF STRINILGHA QQGGSPTPFD RNMGTKLAAR 700
ALEYIITQIK ESMVNGVVST KSPERATLLG LTGRRVVFTP VEELAAETDF 750
DKRLPCDQWW LKLRPLLRIL AKHTSIYHTE AMEDTEDYD 789
Length:789
Mass (Da):87,184
Last modified:November 1, 1996 - v1
Checksum:i1245288AE2289DCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59805 mRNA. Translation: AAA29181.1.
PIRiA45617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59805 mRNA. Translation: AAA29181.1 .
PIRi A45617.

3D structure databases

ProteinModelPortali Q27665.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.

Entry informationi

Entry nameiPFKA_HAECO
AccessioniPrimary (citable) accession number: Q27665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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