Q27665 (K6PF_HAECO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:

PFK

Organism

Haemonchus contortus (Barber pole worm)

Taxonomic identifier

6289 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Strongylida › Trichostrongyloidea › Haemonchidae › Haemonchinae › Haemonchus

Protein attributes

Sequence length	789 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 789	789	6-phosphofructokinase		PRO_0000112032
Regions
	Nucleotide binding	51 – 55	5	ATP By similarity
	Nucleotide binding	209 – 213	5	ATP By similarity
	Nucleotide binding	226 – 242	17	ATP By similarity
Sites
	Active site	182	1	Proton acceptor By similarity
	Binding site	217	1	Substrate By similarity
	Binding site	307	1	Substrate By similarity
	Binding site	313	1	Substrate By similarity
	Binding site	316	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27665 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1245288AE2289DCF	FASTA	789	87,184
	10 20 30 40 50 60 MSLKRNIRRL DSIVPTAGRE GSDIQFNLYK GRGVAVFTSG GDSQGMNGAV HSVVRMGIYL 70 80 90 100 110 120 GCKVCFINEG YQGMVDGGDN IVEASWNSGS DIIQKGGTII GSARCTDLRQ REGRMKAAYN 130 140 150 160 170 180 LIEKGITNLV VIGGDGSLIG ANQFRKDWPG LVKELVDTKK ITPEAAKSYP NIQIVGLVGS 190 200 210 220 230 240 IDNDFCGTDM TIGTDSALQR IIESIDAVVA TAQSHQRAFV VEVMGRHCGY LALVAALACE 250 260 270 280 290 300 ADFCFIPEWP PPVNWREILC KKLQEMRAEG QRLNIIVVAE DDDRDGTPIS SDLVKDVVAK 310 320 330 340 350 360 TLKYDTRVTV LGHVQRGGSP SAFDRLLGCR MGAEAVLALM EMNEESEPCV ISIMVTRWYV 370 380 390 400 410 420 PLMQCVERTK AVQKAMSEKD WELAVKLRGR SFQRNLETYK LLTKLRTVEK DNLSGGQNFN 430 440 450 460 470 480 VAVMNVGAPA GGMNAAVRSF VRMAIVPSLY SLRYEDSFEG LANGAFKKFQ WGDVTNWVMH 490 500 510 520 530 540 GGSFLGTQKQ LPNEKNVPLI AEQLRKHNIQ ALLLVGGFEA YHSTLILSKN REKYPEFCIP 550 560 570 580 590 600 LCVIPCTISN NVPGTSISLG SDTAINEICT MIDKIKQSAT GTKRRVFIIE TMGGYCGYLA 610 620 630 640 650 660 TLSALASGAD NAYIFEEKFT VEISLRRGSH RCKMAQGVQR YLIVRNEYAN KNFTTEFVKQ 670 680 690 700 710 720 LFAEEGKGEF STRINILGHA QQGGSPTPFD RNMGTKLAAR ALEYIITQIK ESMVNGVVST 730 740 750 760 770 780 KSPERATLLG LTGRRVVFTP VEELAAETDF DKRLPCDQWW LKLRPLLRIL AKHTSIYHTE AMEDTEDYD

References

[1]	"Cloning of a cDNA encoding phosphofructokinase from Haemonchus contortus." Klein R.D., Faureau M.A., Winterrowd C.A., Hatzenbuhler N.T., Shea M.H., Nulf S.C., Geary T.G., Olson E.R. Mol. Biochem. Parasitol. 48:17-26(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59805 mRNA. Translation: AAA29181.1.
PIR	A45617.
3D structure databases
ProteinModelPortal	Q27665.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_HAECO

Accession

Primary (citable) accession number: Q27665

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)