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Q27665

- PFKA_HAECO

UniProt

Q27665 - PFKA_HAECO

Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Haemonchus contortus (Barber pole worm)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.1 PublicationUniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411ATP; via amide nitrogenUniRule annotation
    Metal bindingi135 – 1351Magnesium; catalyticUniRule annotation
    Active sitei182 – 1821Proton acceptorUniRule annotation
    Binding sitei217 – 2171Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei280 – 2801SubstrateUniRule annotation
    Binding sitei307 – 3071Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei489 – 4891Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei585 – 5851Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei647 – 6471Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei673 – 6731Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei753 – 7531Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 1052ATPUniRule annotation
    Nucleotide bindingi134 – 1374ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PFK
    OrganismiHaemonchus contortus (Barber pole worm)
    Taxonomic identifieri6289 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 789789ATP-dependent 6-phosphofructokinasePRO_0000112032Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ27665.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 404404N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni180 – 1823Substrate bindingUniRule annotation
    Regioni224 – 2263Substrate bindingUniRule annotation
    Regioni313 – 3164Substrate bindingUniRule annotation
    Regioni405 – 41915Interdomain linkerAdd
    BLAST
    Regioni420 – 789370C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni547 – 5515Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni592 – 5943Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni679 – 6824Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27665-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLKRNIRRL DSIVPTAGRE GSDIQFNLYK GRGVAVFTSG GDSQGMNGAV    50
    HSVVRMGIYL GCKVCFINEG YQGMVDGGDN IVEASWNSGS DIIQKGGTII 100
    GSARCTDLRQ REGRMKAAYN LIEKGITNLV VIGGDGSLIG ANQFRKDWPG 150
    LVKELVDTKK ITPEAAKSYP NIQIVGLVGS IDNDFCGTDM TIGTDSALQR 200
    IIESIDAVVA TAQSHQRAFV VEVMGRHCGY LALVAALACE ADFCFIPEWP 250
    PPVNWREILC KKLQEMRAEG QRLNIIVVAE DDDRDGTPIS SDLVKDVVAK 300
    TLKYDTRVTV LGHVQRGGSP SAFDRLLGCR MGAEAVLALM EMNEESEPCV 350
    ISIMVTRWYV PLMQCVERTK AVQKAMSEKD WELAVKLRGR SFQRNLETYK 400
    LLTKLRTVEK DNLSGGQNFN VAVMNVGAPA GGMNAAVRSF VRMAIVPSLY 450
    SLRYEDSFEG LANGAFKKFQ WGDVTNWVMH GGSFLGTQKQ LPNEKNVPLI 500
    AEQLRKHNIQ ALLLVGGFEA YHSTLILSKN REKYPEFCIP LCVIPCTISN 550
    NVPGTSISLG SDTAINEICT MIDKIKQSAT GTKRRVFIIE TMGGYCGYLA 600
    TLSALASGAD NAYIFEEKFT VEISLRRGSH RCKMAQGVQR YLIVRNEYAN 650
    KNFTTEFVKQ LFAEEGKGEF STRINILGHA QQGGSPTPFD RNMGTKLAAR 700
    ALEYIITQIK ESMVNGVVST KSPERATLLG LTGRRVVFTP VEELAAETDF 750
    DKRLPCDQWW LKLRPLLRIL AKHTSIYHTE AMEDTEDYD 789
    Length:789
    Mass (Da):87,184
    Last modified:November 1, 1996 - v1
    Checksum:i1245288AE2289DCF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59805 mRNA. Translation: AAA29181.1.
    PIRiA45617.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59805 mRNA. Translation: AAA29181.1 .
    PIRi A45617.

    3D structure databases

    ProteinModelPortali Q27665.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.

    Entry informationi

    Entry nameiPFKA_HAECO
    AccessioniPrimary (citable) accession number: Q27665
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3