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Protein

ATP-dependent 6-phosphofructokinase

Gene

PPi-PFK

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation1 Publication

Enzyme regulationi

Activated by nucleoside triphosphates. Inhibited by phosphoenolpyruvate. EDTA and biphosphonates play the role of inhbitors of kinase activity.1 Publication

Kineticsi

  1. KM=0.12 mM for ATP
  2. KM=0.067 mM for GTP
  3. KM=0.136 mM for ITP
  4. KM=1.93 mM for UTP
  5. KM=3.6 mM for CTP
  6. KM=3.8 mM for fructose 6-phosphate

    pH dependencei

    Optimum pH is 6-7.

    Pathwayi: glycolysis

    This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Glucose-6-phosphate isomerase (GPI), Glucose-6-phosphate isomerase (G6PI), Glucose-6-phosphate isomerase (GPI), Glucose-6-phosphate isomerase (GPI)
    3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase, Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (EHI_000730), ATP-dependent 6-phosphofructokinase (PPi-PFK)
    4. no protein annotated in this organism
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901ATP; via amide nitrogenUniRule annotation
    Metal bindingi181 – 1811Magnesium; catalyticUniRule annotation
    Sitei182 – 1821Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei211 – 2111Proton acceptorUniRule annotation
    Binding sitei307 – 3071SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi155 – 1562ATPUniRule annotation
    Nucleotide bindingi180 – 1834ATPUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.90. 2080.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PPi-PFK
    ORF Names:EHI_103590
    OrganismiEntamoeba histolytica
    Taxonomic identifieri5759 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
    Proteomesi
    • UP000001926 Componenti: Partially assembled WGS sequence

    Organism-specific databases

    EuPathDBiAmoebaDB:EHI_103590.

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 436436ATP-dependent 6-phosphofructokinasePRO_0000423021Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Aggregates to a homotetramer after activation by ATP.1 Publication

    Protein-protein interaction databases

    STRINGi5759.rna_EHI_103590-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27651.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni209 – 2113Substrate bindingUniRule annotation
    Regioni254 – 2563Substrate bindingUniRule annotation
    Regioni362 – 3654Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiKOG2440. Eukaryota.
    COG0205. LUCA.
    KOiK00850.
    OMAiRTNPQKV.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
    InterProiIPR022953. ATP_PFK.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFK_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q27651-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVKRRDHIL IPKNPDAPLP SLKIEEVGEC TIDNIYASPE PFVNGMTMKL
    60 70 80 90 100
    SAVKNHGIER DSGEVELAGP MEKIFYNPET TKVAIVTCGG LCPGLNNVIR
    110 120 130 140 150
    GLVLNLYNRY HVNNIFGLRW GYEGLVPELS EVQRLTPEIV SDIHQKGGSI
    160 170 180 190 200
    LGTSRGAQSP EVMAQFLIDN NFNILFTLGG DGTLRGANAI NKELRRRKVP
    210 220 230 240 250
    ITVVGIPKTI DNDICYTDST FGFQTAVGLS QEAINAVHSE AKSAKNGIGI
    260 270 280 290 300
    VRLMGRDAGF IALYASLANG DANLVLIPEI DIPITQICEF VGKRIMSKGH
    310 320 330 340 350
    VVIVVAEGAL QNQKPKDLDL GTDKSGNILH WDSINYLRDS ITKYLKSIGI
    360 370 380 390 400
    EEHTIKFVDP SYMIRSAPCS AADAHFCMCL ANAAVHVAMA GKTGLVICHH
    410 420 430
    HNNFVSVPID RTSYYIKRVN TDGPLYTMMT AIEKPK
    Length:436
    Mass (Da):47,670
    Last modified:July 9, 2014 - v2
    Checksum:iBFD2E9639DAA1138
    GO

    Sequence cautioni

    The sequence AAA92671 differs from that shown. Reason: Frameshift at position 417. Curated
    The sequence AAA92671 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091R → A in CAA57659 (PubMed:8645233).Curated
    Sequence conflicti430 – 4301T → S in CAA57659 (PubMed:8645233).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X82173 mRNA. Translation: CAA57659.1.
    DS571158 Genomic DNA. Translation: EAL47987.1.
    U12513 Genomic DNA. Translation: AAA92671.1. Sequence problems.
    PIRiS49458.
    RefSeqiXP_653373.1. XM_648281.1.

    Genome annotation databases

    EnsemblProtistsirna_EHI_103590-1; rna_EHI_103590-1; EHI_103590.
    GeneIDi3407680.
    KEGGiehi:EHI_103590.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X82173 mRNA. Translation: CAA57659.1.
    DS571158 Genomic DNA. Translation: EAL47987.1.
    U12513 Genomic DNA. Translation: AAA92671.1. Sequence problems.
    PIRiS49458.
    RefSeqiXP_653373.1. XM_648281.1.

    3D structure databases

    ProteinModelPortaliQ27651.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi5759.rna_EHI_103590-1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsirna_EHI_103590-1; rna_EHI_103590-1; EHI_103590.
    GeneIDi3407680.
    KEGGiehi:EHI_103590.

    Organism-specific databases

    EuPathDBiAmoebaDB:EHI_103590.

    Phylogenomic databases

    eggNOGiKOG2440. Eukaryota.
    COG0205. LUCA.
    KOiK00850.
    OMAiRTNPQKV.

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.
    BRENDAi2.7.1.90. 2080.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
    InterProiIPR022953. ATP_PFK.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFK_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPFKA_ENTHI
    AccessioniPrimary (citable) accession number: Q27651
    Secondary accession number(s): C4LVK3, Q24812
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: July 9, 2014
    Last modified: September 7, 2016
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    Was originally thought to be a PPi-dependent phosphofructokinase (PubMed:8645233), but it has later been shown that the enzyme does not posses PPi-dependent activity and instead is an ATP-dependent phosphofructokinase (PubMed:11262402).2 Publications

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.