Reviewed, UniProtKB/Swiss-Prot Q27650 (LYS4_ENTHI)

Names and origin

Protein names

Recommended name:
    Lysozyme
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase

Gene names

Name:

LYS4

Organism

Entamoeba histolytica

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has antibacterial activity against Gram-positive bacteria. No activity against S.aureus.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subcellular location	Cytoplasmic granule.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.
Biophysicochemical properties	pH dependence: Optimally active at acid pH.

Ontologies

Keywords
Molecular function	Antibiotic Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Lysozyme		PRO_0000084536
Sites
	Active site	98	1	By similarity
Experimental info
	Sequence uncertainty	17	1

Sequences

	Sequence		Length	Mass (Da)
	Q27650-1 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 1AF3B73CDAEBBCE9	FASTA	198	22,379
	10 20 30 40 50 60 KLGIDVSQPT STSSFTCLRN KGFTTMVIVR AWKSTGSFDT NAPQTLKNAN AAGFSIENSD 70 80 90 100 110 120 VYYYPCISCG NMAGQVRTFW QKVGQYSLKV KRVWFDIEGT WTSSVSTNQN YLMQMMNEAR 130 140 150 160 170 180 AIGIVHGIYG SKYYWGNLFG SSYKYRYRSS TPLWYPHYDN SPSFSDFSSF GGWTSPSMKQ 190 YRGDVSVCSA GVDYNYKP

References

[1]

"Purification and molecular cloning of a major antibacterial protein of the protozoan parasite Entamoeba histolytica with lysozyme-like properties." Jacobs T., Leippe M. Eur. J. Biochem. 231:831-838(1995) [PubMed: 7649184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-198, PROTEIN SEQUENCE OF 1-47; 133-144 AND 180-197. Strain: HM-1:IMSS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87610 mRNA. Translation: CAA60916.1.
PIR	S66599.
3D structure databases
SMR	Q27650. Positions 2-196.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.17. 323.
Family and domain databases
InterPro	IPR002053. Glyco_hydro_25. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01183. Glyco_hydro_25. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS4_ENTHI

Accession

Primary (citable) accession number: Q27650

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 1, 1998
Last modified:	January 19, 2010
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)