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Q27650

- LYS4_ENTHI

UniProt

Q27650 - LYS4_ENTHI

Protein

Lysozyme

Gene

LYS4

Organism
Entamoeba histolytica
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (01 Nov 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Has antibacterial activity against Gram-positive bacteria. No activity against S.aureus.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    pH dependencei

    Optimally active at acid pH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981By similarity

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell wall macromolecule catabolic process Source: InterPro
    3. cytolysis Source: UniProtKB-KW
    4. defense response to bacterium Source: UniProtKB-KW
    5. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysozyme (EC:3.2.1.17)
    Alternative name(s):
    1,4-beta-N-acetylmuramidase
    Gene namesi
    Name:LYS4
    OrganismiEntamoeba histolytica
    Taxonomic identifieri5759 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

    Subcellular locationi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 198198LysozymePRO_0000084536Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ27650.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 25 family.Curated

    Phylogenomic databases

    eggNOGiNOG75899.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR002053. Glyco_hydro_25.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01183. Glyco_hydro_25. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q27650-1 [UniParc]FASTAAdd to Basket

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    KLGIDVSQPT STSSFTCLRN KGFTTMVIVR AWKSTGSFDT NAPQTLKNAN    50
    AAGFSIENSD VYYYPCISCG NMAGQVRTFW QKVGQYSLKV KRVWFDIEGT 100
    WTSSVSTNQN YLMQMMNEAR AIGIVHGIYG SKYYWGNLFG SSYKYRYRSS 150
    TPLWYPHYDN SPSFSDFSSF GGWTSPSMKQ YRGDVSVCSA GVDYNYKP 198
    Length:198
    Mass (Da):22,379
    Last modified:November 1, 1998 - v2
    Checksum:i1AF3B73CDAEBBCE9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence uncertaintyi17 – 171

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87610 mRNA. Translation: CAA60916.1.
    PIRiS66599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87610 mRNA. Translation: CAA60916.1 .
    PIRi S66599.

    3D structure databases

    ProteinModelPortali Q27650.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG75899.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR002053. Glyco_hydro_25.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01183. Glyco_hydro_25. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification and molecular cloning of a major antibacterial protein of the protozoan parasite Entamoeba histolytica with lysozyme-like properties."
      Jacobs T., Leippe M.
      Eur. J. Biochem. 231:831-838(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-198, PROTEIN SEQUENCE OF 1-47; 133-144 AND 180-197.
      Strain: ATCC 30459 / HM-1:IMSS.

    Entry informationi

    Entry nameiLYS4_ENTHI
    AccessioniPrimary (citable) accession number: Q27650
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3