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Protein

Cytochrome P450 4e2

Gene

Cyp4e2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.By similarity

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei307 – 3071Heme (covalent; via 1 link)By similarity
Metal bindingi444 – 4441Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4e2 (EC:1.14.-.-)
Alternative name(s):
CYPIVE2
Gene namesi
Name:Cyp4e2
ORF Names:CG2060
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0014469. Cyp4e2.

Subcellular locationi

GO - Cellular componenti

  • endomembrane system Source: FlyBase
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Cytochrome P450 4e2PRO_0000051841Add
BLAST

Proteomic databases

PaxDbiQ27606.

Expressioni

Gene expression databases

BgeeiQ27606.
GenevisibleiQ27606. DM.

Interactioni

Protein-protein interaction databases

BioGridi61675. 4 interactions.
DIPiDIP-23009N.
IntActiQ27606. 2 interactions.
MINTiMINT-1667632.
STRINGi7227.FBpp0087824.

Structurei

3D structure databases

ProteinModelPortaliQ27606.
SMRiQ27606. Positions 36-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000263743.
InParanoidiQ27606.
KOiK15001.
OMAiFSWWHQY.
OrthoDBiEOG7CNZFK.
PhylomeDBiQ27606.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27606-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFVLYIFLA LPLLLVAYLE LSTFRRRRVL NKFNGPRGLP LMGNAHQMGK
60 70 80 90 100
NPSEILDTVF SWWHQYGKDN FVFWIGTYSN VLVTSSKYLE FILSSQTLIT
110 120 130 140 150
KSDIYQLTHP WLGLGLLTST GSKWHKHRKM ITPAFHFNIL QDFHEVMNEN
160 170 180 190 200
STKFIKHLKT VAAGDNIFDF QEQAHYLTLD VICDTAMGVS INAMENRSSS
210 220 230 240 250
IVQAFKDMCY NINMRAFHPL KRNELLYRLA PDYPAYSRTL KTLQDFTNEI
260 270 280 290 300
IAKRIEAHKS GAVSTNAGDE FTRKKMAFLD TLLSSTIDGR PLNSKELYEE
310 320 330 340 350
VSTFMFEGHD TTTSGVSFAV YLLSRHQDEQ RKLFKEQREV MGNSELGRDA
360 370 380 390 400
TFQEISQMKY LDLFIKEAQR VYPSVPFIGR FTEKDYVIDG DLVPKGTTLN
410 420 430 440 450
LGLVMLGYNE KVFKDPHKFR PERFELEKPG PFEYVPFSAG PRNCIGQKFA
460 470 480 490 500
LLEIKTVVSK IIRNFEVLPA LDELVSKDGY ISTTIGLPDA ERKKRDPYRH
510 520
KYDPILSAVL TLKSENGLYI RLKERH
Length:526
Mass (Da):60,693
Last modified:December 1, 2000 - v2
Checksum:iA13DD43C4F4F4D52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2561E → K in CAA60032 (Ref. 5) Curated
Sequence conflicti302 – 3021S → D in CAA60032 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56957 mRNA. Translation: AAC47424.1.
AE013599 Genomic DNA. Translation: AAF59091.1.
AY058518 mRNA. Translation: AAL13747.1.
X86076 mRNA. Translation: CAA60032.1.
U34332 mRNA. Translation: AAA80666.1.
PIRiJC5236.
S57646.
S70622.
RefSeqiNP_001286196.1. NM_001299267.1.
NP_477117.2. NM_057769.4.
UniGeneiDm.528.

Genome annotation databases

EnsemblMetazoaiFBtr0088745; FBpp0087824; FBgn0014469.
FBtr0339278; FBpp0308387; FBgn0014469.
GeneIDi35822.
KEGGidme:Dmel_CG2060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56957 mRNA. Translation: AAC47424.1.
AE013599 Genomic DNA. Translation: AAF59091.1.
AY058518 mRNA. Translation: AAL13747.1.
X86076 mRNA. Translation: CAA60032.1.
U34332 mRNA. Translation: AAA80666.1.
PIRiJC5236.
S57646.
S70622.
RefSeqiNP_001286196.1. NM_001299267.1.
NP_477117.2. NM_057769.4.
UniGeneiDm.528.

3D structure databases

ProteinModelPortaliQ27606.
SMRiQ27606. Positions 36-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61675. 4 interactions.
DIPiDIP-23009N.
IntActiQ27606. 2 interactions.
MINTiMINT-1667632.
STRINGi7227.FBpp0087824.

Proteomic databases

PaxDbiQ27606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088745; FBpp0087824; FBgn0014469.
FBtr0339278; FBpp0308387; FBgn0014469.
GeneIDi35822.
KEGGidme:Dmel_CG2060.

Organism-specific databases

CTDi35822.
FlyBaseiFBgn0014469. Cyp4e2.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000263743.
InParanoidiQ27606.
KOiK15001.
OMAiFSWWHQY.
OrthoDBiEOG7CNZFK.
PhylomeDBiQ27606.

Miscellaneous databases

GenomeRNAii35822.
PROiQ27606.

Gene expression databases

BgeeiQ27606.
GenevisibleiQ27606. DM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a Drosophila Cyp4e2 cytochrome P450-encoding cDNA."
    Pittendrigh B.R., Mocelin G., Andreev O., ffrench-Constant R.H.
    Gene 179:295-296(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-526.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Cloning and expression study of CYP4E2 a novel P450 gene in Drosophila."
    Amichot M., Brun A., Cuany A., Lemouel T., Berge J.-B.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-444.
    Strain: Raleigh DDTR.
  6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 312-435.
    Strain: Haag-79.

Entry informationi

Entry nameiCP4E2_DROME
AccessioniPrimary (citable) accession number: Q27606
Secondary accession number(s): Q24130, Q24291, Q9V4T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 1, 2000
Last modified: July 6, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.