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Protein

NADPH--cytochrome P450 reductase

Gene

Cpr

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome p450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome b5 (By similarity). May function to clear the olfactory organ (antennae) from accumulating chemicals.UniRule annotation1 Publication

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212FMNUniRule annotation1
Binding sitei302NADPUniRule annotation1
Binding sitei482FADUniRule annotation1
Binding sitei537NADPUniRule annotation1
Binding sitei640NADPUniRule annotation1
Binding sitei678FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 95FMNUniRule annotation6
Nucleotide bindingi142 – 145FMNUniRule annotation4
Nucleotide bindingi177 – 186FMNUniRule annotation10
Nucleotide bindingi458 – 461FADUniRule annotation4
Nucleotide bindingi476 – 478FADUniRule annotation3
Nucleotide bindingi492 – 495FADUniRule annotation4
Nucleotide bindingi597 – 598NADPUniRule annotation2
Nucleotide bindingi603 – 607NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

  • cuticle hydrocarbon biosynthetic process Source: FlyBase

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

ReactomeiR-DME-211897 Cytochrome P450 - arranged by substrate type

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:Cpr
ORF Names:CG11567
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015623 Cpr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21LumenalUniRule annotationAdd BLAST21
Transmembranei22 – 42HelicalUniRule annotationAdd BLAST21
Topological domaini43 – 679CytoplasmicUniRule annotationAdd BLAST637

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676021 – 679NADPH--cytochrome P450 reductaseAdd BLAST679

Proteomic databases

PaxDbiQ27597
PRIDEiQ27597

Expressioni

Tissue specificityi

High in antennae.1 Publication

Developmental stagei

Embryos and adults.

Gene expression databases

BgeeiFBgn0015623
ExpressionAtlasiQ27597 baseline and differential
GenevisibleiQ27597 DM

Interactioni

Protein-protein interaction databases

BioGridi60040, 1 interactor
DIPiDIP-19958N
STRINGi7227.FBpp0078880

Structurei

3D structure databases

ProteinModelPortaliQ27597
SMRiQ27597
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 228Flavodoxin-likeUniRule annotationAdd BLAST145
Domaini283 – 523FAD-binding FR-typeUniRule annotationAdd BLAST241

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
COG0369 LUCA
GeneTreeiENSGT00840000129757
InParanoidiQ27597
KOiK00327
OrthoDBiEOG091G02VT
PhylomeDBiQ27597

Family and domain databases

Gene3Di1.20.990.10, 4 hits
3.40.50.360, 1 hit
HAMAPiMF_03212 NCPR, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR023208 P450R
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000208 P450R, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q27597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEQTIDGA AAIPSGGGDE PFLGLLDVAL LAVLIGGAAF YFLRSRKKEE
60 70 80 90 100
EPTRSYSIQP TTVCTTSASD NSFIKKLKAS GRSLVVFYGS QTGTGEEFAG
110 120 130 140 150
RLAKEGIRYR LKGMVADPEE CDMEELLQLK DIDNSLAVFC LATYGEGDPT
160 170 180 190 200
DNAMEFYEWI TSGDVDLSGL NYAVFGLGNK TYEHYNKVAI YVDKRLEELG
210 220 230 240 250
ANRVFELGLG DDDANIEDDF ITWKDRFWPA VCDHFGIEGG GEEVLIRQYR
260 270 280 290 300
LLEQPDVQPD RIYTGEIARL HSIQNQRPPF DAKNPFLAPI KVNRELHKGG
310 320 330 340 350
GRSCMHIELS IEGSKMRYDA GDHVAMFPVN DKSLVEKLGQ LCNADLDTVF
360 370 380 390 400
SLINTDTDSS KKHPFPCPTT YRTALTHYLE ITAIPRTHIL KELAEYCTDE
410 420 430 440 450
KEKELLRSMA SISPEGKEKY QSWIQDACRN IVHILEDIKS CRPPIDHVCE
460 470 480 490 500
LLPRLQPRYY SISSSAKLHP TDVHVTAVLV EYKTPTGRIN KGVATTYLKN
510 520 530 540 550
KQPQGSEEVK VPVFIRKSQF RLPTKPETPI IMVGPGTGLA PFRGFIQERQ
560 570 580 590 600
FLRDEGKTVG ESILYFGCRK RSEDYIYESE LEEWVKKGTL NLKAAFSRDQ
610 620 630 640 650
GKKVYVQHLL EQDADLIWNV IGENKGHFYI CGDAKNMAVD VRNILVKILS
660 670
TKGNMSEADA VQYIKKMEAQ KRYSADVWS
Length:679
Mass (Da):76,347
Last modified:December 1, 2000 - v2
Checksum:iC6387C111A0EDB4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 39AA → VT in CAA63639 (PubMed:9168130).Curated2
Sequence conflicti45S → T in CAA63639 (PubMed:9168130).Curated1
Sequence conflicti132I → T in CAA63639 (PubMed:9168130).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93090 mRNA Translation: CAA63639.1
AE014134 Genomic DNA Translation: AAF52367.1
AY052000 mRNA Translation: AAK93424.1
RefSeqiNP_001260128.1, NM_001273199.1
NP_001260129.1, NM_001273200.1
NP_477158.1, NM_057810.4
UniGeneiDm.6997

Genome annotation databases

EnsemblMetazoaiFBtr0079250; FBpp0078880; FBgn0015623
FBtr0331223; FBpp0303665; FBgn0015623
FBtr0331224; FBpp0303666; FBgn0015623
GeneIDi33883
KEGGidme:Dmel_CG11567

Similar proteinsi

Entry informationi

Entry nameiNCPR_DROME
AccessioniPrimary (citable) accession number: Q27597
Secondary accession number(s): Q9VMF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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