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Protein

NADPH--cytochrome P450 reductase

Gene

Cpr

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome p450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome b5 (By similarity). May function to clear the olfactory organ (antennae) from accumulating chemicals.IEP:1 Publication

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.IEP:

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212FMNIEP:1
Binding sitei302NADPIEP:1
Binding sitei482FADIEP:1
Binding sitei537NADPIEP:1
Binding sitei640NADPIEP:1
Binding sitei678FADIEP:1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 95FMNIEP:6
Nucleotide bindingi142 – 145FMNIEP:4
Nucleotide bindingi177 – 186FMNIEP:10
Nucleotide bindingi458 – 461FADIEP:4
Nucleotide bindingi476 – 478FADIEP:3
Nucleotide bindingi492 – 495FADIEP:4
Nucleotide bindingi597 – 598NADPIEP:2
Nucleotide bindingi603 – 607NADPIEP:5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

ReactomeiR-DME-211897. Cytochrome P450 - arranged by substrate type.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseIEP: (EC:1.6.2.4IEP:)
Short name:
CPRIEP:
Short name:
P450RIEP:
Gene namesi
Name:Cpr
ORF Names:CG11567
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015623. Cpr.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21LumenalIEP:Add BLAST21
Transmembranei22 – 42HelicalIEP:Add BLAST21
Topological domaini43 – 679CytoplasmicIEP:Add BLAST637

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676021 – 679NADPH--cytochrome P450 reductaseAdd BLAST679

Proteomic databases

PaxDbiQ27597.
PRIDEiQ27597.

Expressioni

Tissue specificityi

High in antennae.1 Publication

Developmental stagei

Embryos and adults.

Gene expression databases

BgeeiFBgn0015623.
ExpressionAtlasiQ27597. differential.
GenevisibleiQ27597. DM.

Interactioni

Protein-protein interaction databases

BioGridi60040. 1 interactor.
DIPiDIP-19958N.
MINTiMINT-863368.
STRINGi7227.FBpp0078880.

Structurei

3D structure databases

ProteinModelPortaliQ27597.
SMRiQ27597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 228Flavodoxin-likeIEP:Add BLAST145
Domaini283 – 523FAD-binding FR-typeIEP:Add BLAST241

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.IEP:
In the N-terminal section; belongs to the flavodoxin family.IEP:
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.IEP:

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
InParanoidiQ27597.
KOiK00327.
OMAiTKMDVAF.
OrthoDBiEOG091G02VT.
PhylomeDBiQ27597.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiView protein in InterPro
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_sf.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEQTIDGA AAIPSGGGDE PFLGLLDVAL LAVLIGGAAF YFLRSRKKEE
60 70 80 90 100
EPTRSYSIQP TTVCTTSASD NSFIKKLKAS GRSLVVFYGS QTGTGEEFAG
110 120 130 140 150
RLAKEGIRYR LKGMVADPEE CDMEELLQLK DIDNSLAVFC LATYGEGDPT
160 170 180 190 200
DNAMEFYEWI TSGDVDLSGL NYAVFGLGNK TYEHYNKVAI YVDKRLEELG
210 220 230 240 250
ANRVFELGLG DDDANIEDDF ITWKDRFWPA VCDHFGIEGG GEEVLIRQYR
260 270 280 290 300
LLEQPDVQPD RIYTGEIARL HSIQNQRPPF DAKNPFLAPI KVNRELHKGG
310 320 330 340 350
GRSCMHIELS IEGSKMRYDA GDHVAMFPVN DKSLVEKLGQ LCNADLDTVF
360 370 380 390 400
SLINTDTDSS KKHPFPCPTT YRTALTHYLE ITAIPRTHIL KELAEYCTDE
410 420 430 440 450
KEKELLRSMA SISPEGKEKY QSWIQDACRN IVHILEDIKS CRPPIDHVCE
460 470 480 490 500
LLPRLQPRYY SISSSAKLHP TDVHVTAVLV EYKTPTGRIN KGVATTYLKN
510 520 530 540 550
KQPQGSEEVK VPVFIRKSQF RLPTKPETPI IMVGPGTGLA PFRGFIQERQ
560 570 580 590 600
FLRDEGKTVG ESILYFGCRK RSEDYIYESE LEEWVKKGTL NLKAAFSRDQ
610 620 630 640 650
GKKVYVQHLL EQDADLIWNV IGENKGHFYI CGDAKNMAVD VRNILVKILS
660 670
TKGNMSEADA VQYIKKMEAQ KRYSADVWS
Length:679
Mass (Da):76,347
Last modified:December 1, 2000 - v2
Checksum:iC6387C111A0EDB4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 39AA → VT in CAA63639 (PubMed:9168130).IKR:2
Sequence conflicti45S → T in CAA63639 (PubMed:9168130).IKR:1
Sequence conflicti132I → T in CAA63639 (PubMed:9168130).IKR:1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93090 mRNA. Translation: CAA63639.1.
AE014134 Genomic DNA. Translation: AAF52367.1.
AY052000 mRNA. Translation: AAK93424.1.
RefSeqiNP_001260128.1. NM_001273199.1.
NP_001260129.1. NM_001273200.1.
NP_477158.1. NM_057810.4.
UniGeneiDm.6997.

Genome annotation databases

EnsemblMetazoaiFBtr0079250; FBpp0078880; FBgn0015623.
FBtr0331223; FBpp0303665; FBgn0015623.
FBtr0331224; FBpp0303666; FBgn0015623.
GeneIDi33883.
KEGGidme:Dmel_CG11567.

Similar proteinsi

Entry informationi

Entry nameiNCPR_DROME
AccessioniPrimary (citable) accession number: Q27597
Secondary accession number(s): Q9VMF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 22, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families