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Q27575

- PSA73_DROME

UniProt

Q27575 - PSA73_DROME

Protein

Proteasome subunit alpha type-7-1B

Gene

Prosalpha4T2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (08 Dec 2000)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha type-7-1B (EC:3.4.25.1)
    Alternative name(s):
    Testis-specific alpha4-t2 proteasome subunit
    Testis-specific proteasome 28 kDa subunit 1B
    Gene namesi
    Name:Prosalpha4T2
    Synonyms:Pros28.1B
    ORF Names:CG4569
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0017556. Prosalpha4T2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 252252Proteasome subunit alpha type-7-1BPRO_0000124155Add
    BLAST

    Expressioni

    Tissue specificityi

    Testis specific.

    Gene expression databases

    BgeeiQ27575.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ27575.
    SMRiQ27575. Positions 5-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00550000074753.
    InParanoidiQ27575.
    KOiK02731.
    OMAiRTKDCIV.
    OrthoDBiEOG71VSTG.
    PhylomeDBiQ27575.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27575-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQRYDRAVT IYSPDGHLLQ VEYAQEAVRR GSTVMGLRTN NAIVIGVEKR    50
    SVGDLQEERM VRKICMLDDH VVMTFSGLTA DARILVSRAQ MEAQSHRLNF 100
    EKPTTVEYIT RYIAQLKQNY TQSNGRRPFG LSCLVGGFDE DGTPHLFQTD 150
    PSGIFYEWRA NTTGRSSQPV RDYMEKHADE ILTIADEAAA IKHIVRTLVS 200
    VSSLNHTQME VAVLKYRQPL RMIDHQVLAD LERTVRREIE DEAEASRRPR 250
    AP 252
    Length:252
    Mass (Da):28,720
    Last modified:December 8, 2000 - v2
    Checksum:i00116788A4813146
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 42QR → HG in AAC47281. (PubMed:8878681)Curated
    Sequence conflicti188 – 1903AAA → RG in AAC47281. (PubMed:8878681)Curated
    Sequence conflicti215 – 2151K → N in AAL90194. (PubMed:12537569)Curated
    Sequence conflicti243 – 2497AEASRRP → PSESA in AAC47281. (PubMed:8878681)Curated
    Sequence conflicti245 – 2451A → V in AAL90194. (PubMed:12537569)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti45 – 451I → L in strain: S-23.
    Natural varianti191 – 1911I → V in strain: S-23.
    Natural varianti213 – 2131V → E in strain: S-23.
    Natural varianti241 – 2411D → A in strain: S-23.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46009 Genomic DNA. Translation: AAC47281.1.
    AY542417 Genomic DNA. Translation: AAS86225.1.
    AY542418 Genomic DNA. Translation: AAS86226.1.
    AY542421 Genomic DNA. Translation: AAS86229.1.
    AY542422 Genomic DNA. Translation: AAS86230.1.
    AY542423 Genomic DNA. Translation: AAS86231.1.
    AY542424 Genomic DNA. Translation: AAS86232.1.
    AY542425 Genomic DNA. Translation: AAS86233.1.
    AY542426 Genomic DNA. Translation: AAS86234.1.
    AY542427 Genomic DNA. Translation: AAS86235.1.
    AE013599 Genomic DNA. Translation: AAF47215.1.
    AY089456 mRNA. Translation: AAL90194.1.
    PIRiS72226.
    RefSeqiNP_611920.1. NM_138076.3.
    UniGeneiDm.772.

    Genome annotation databases

    EnsemblMetazoaiFBtr0072350; FBpp0072257; FBgn0017556.
    GeneIDi37910.
    KEGGidme:Dmel_CG4569.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46009 Genomic DNA. Translation: AAC47281.1 .
    AY542417 Genomic DNA. Translation: AAS86225.1 .
    AY542418 Genomic DNA. Translation: AAS86226.1 .
    AY542421 Genomic DNA. Translation: AAS86229.1 .
    AY542422 Genomic DNA. Translation: AAS86230.1 .
    AY542423 Genomic DNA. Translation: AAS86231.1 .
    AY542424 Genomic DNA. Translation: AAS86232.1 .
    AY542425 Genomic DNA. Translation: AAS86233.1 .
    AY542426 Genomic DNA. Translation: AAS86234.1 .
    AY542427 Genomic DNA. Translation: AAS86235.1 .
    AE013599 Genomic DNA. Translation: AAF47215.1 .
    AY089456 mRNA. Translation: AAL90194.1 .
    PIRi S72226.
    RefSeqi NP_611920.1. NM_138076.3.
    UniGenei Dm.772.

    3D structure databases

    ProteinModelPortali Q27575.
    SMRi Q27575. Positions 5-244.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0072350 ; FBpp0072257 ; FBgn0017556 .
    GeneIDi 37910.
    KEGGi dme:Dmel_CG4569.

    Organism-specific databases

    CTDi 37910.
    FlyBasei FBgn0017556. Prosalpha4T2.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00550000074753.
    InParanoidi Q27575.
    KOi K02731.
    OMAi RTKDCIV.
    OrthoDBi EOG71VSTG.
    PhylomeDBi Q27575.

    Miscellaneous databases

    GenomeRNAii 37910.
    NextBioi 805998.

    Gene expression databases

    Bgeei Q27575.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Duplicated proteasome subunit genes in Drosophila melanogaster encoding testes-specific isoforms."
      Yuan X., Miller M., Belote J.M.
      Genetics 144:147-157(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Testis.
    2. "Rapid evolution through gene duplication and subfunctionalization of the testes-specific alpha4 proteasome subunits in Drosophila."
      Torgerson D.G., Singh R.S.
      Genetics 168:1421-1432(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CPA-129, CPA-46, S-23, Z(H)12, Z(H)16 and Z(H)34.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Testis.

    Entry informationi

    Entry nameiPSA73_DROME
    AccessioniPrimary (citable) accession number: Q27575
    Secondary accession number(s): Q6QH11, Q8T3X2, Q9W162
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 8, 2000
    Last sequence update: December 8, 2000
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3