Reviewed, UniProtKB/Swiss-Prot Q27571 (NOS_DROME)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase
    EC=1.14.13.39
Alternative name(s):
    dNOS

Gene names

Name:	Nos
ORF Names:	CG6713

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Truncated isoforms (isoform 3-isoform 6) are able to form intracellular complexes with the full length protein and serve as dominant negative inhibitors of the enzyme activity. Ref.1 Ref.2 Ref.6
Catalytic activity	L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity.
Enzyme regulation	Stimulated by calcium/calmodulin. Ref.1
Developmental stage	Isoform 3 is expressed in larvae only. Isoform 4, isoform 5, isoform 6 and isoform 10 are expressed throughout development from embryos to adults. Ref.2
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	imaginal disc development Traceable author statement. Source: FlyBase negative regulation of DNA replication Traceable author statement. Source: FlyBase negative regulation of cell proliferation Traceable author statement. Source: FlyBase nitric oxide biosynthetic process Ref.2 Traceable author statement. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of organ growth Traceable author statement. Source: FlyBase synaptogenesis Traceable author statement. Source: FlyBase
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro FMN binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro calmodulin binding Non-traceable author statement. Source: FlyBase electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Non-traceable author statement. Source: FlyBase nitric-oxide synthase activity Ref.1 Ref.2 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Spf45	Q7PL83	1	EBI-250663,EBI-163247

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q27571-1)

Also known as: A; C; dNOS-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q27571-2)

Also known as: dNOS-2; E;

The sequence of this isoform differs from the canonical sequence as follows:
     417-521: Missing.

Isoform 3 (identifier: Q27571-3)

Also known as: dNOS-3;

The sequence of this isoform differs from the canonical sequence as follows:
     201-214: FMHLDDEGRSLLMR → RFFPARRPPVRAAL
     215-1349: Missing.

Isoform 4 (identifier: Q27571-4)

Also known as: B; dNOS-4; F; J;

The sequence of this isoform differs from the canonical sequence as follows:
     736-756: LFSQELYAMRVQESSEHGLQD → VSTPPRKDHTELINGLGPAAF
     757-1349: Missing.

Isoform 5 (identifier: Q27571-5)

Also known as: dNOS-5; H;

The sequence of this isoform differs from the canonical sequence as follows:
     701-704: IYCM → VSLT
     705-1349: Missing.

Isoform 6 (identifier: Q27571-6)

Also known as: dNOS-6; I;

The sequence of this isoform differs from the canonical sequence as follows:
     701-746: IYCMSDYDIS...FSQELYAMRV → CPVGSVSSDP...WQNKISCKHL
     747-1349: Missing.

Isoform 10 (identifier: Q27571-7)

Also known as: dNOS-10; G;

The sequence of this isoform differs from the canonical sequence as follows:
     758-1291: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1349	1349	Nitric oxide synthase		PRO_0000170951
Regions
	Domain	671 – 868	198	Flavodoxin-like
	Domain	928 – 1167	240	FAD-binding FR-type
	Nucleotide binding	814 – 845	32	FMN By similarity
	Nucleotide binding	957 – 968	12	FAD By similarity
	Nucleotide binding	1100 – 1110	11	FAD By similarity
	Nucleotide binding	1175 – 1193	19	NADP By similarity
	Nucleotide binding	1273 – 1287	15	NADP By similarity
	Region	641 – 661	21	Calmodulin-binding Potential
	Compositional bias	24 – 51	28	Gln-rich
	Compositional bias	76 – 79	4	Poly-Gly
	Compositional bias	145 – 156	12	Poly-Gly
Sites
	Metal binding	328	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	201 – 214	14	FMHLD…SLLMR → RFFPARRPPVRAAL in isoform 3.		VSP_003585
	Alternative sequence	215 – 1349	1135	Missing in isoform 3.		VSP_003586
	Alternative sequence	417 – 521	105	Missing in isoform 2.		VSP_003587
	Alternative sequence	701 – 746	46	IYCMS…YAMRV → CPVGSVSSDPQDPVQLGLVV VLVSSTCVCKKHLPAAWQNK ISCKHL in isoform 6.		VSP_003590
	Alternative sequence	701 – 704	4	IYCM → VSLT in isoform 5.		VSP_003588
	Alternative sequence	705 – 1349	645	Missing in isoform 5.		VSP_003589
	Alternative sequence	736 – 756	21	LFSQE…HGLQD → VSTPPRKDHTELINGLGPAA F in isoform 4.		VSP_003592
	Alternative sequence	747 – 1349	603	Missing in isoform 6.		VSP_003591
	Alternative sequence	757 – 1349	593	Missing in isoform 4.		VSP_003593
	Alternative sequence	758 – 1291	534	Missing in isoform 10.		VSP_003594
Experimental info
	Sequence conflict	20	1	S → A in AAC46882. Ref.1
	Sequence conflict	24	1	Q → QQ in AAC46882. Ref.1
	Sequence conflict	105	1	S → L in AAC46882. Ref.1
	Sequence conflict	287	1	S → C in AAC46882. Ref.1
	Sequence conflict	373	1	T → S in AAF25682. Ref.2
	Sequence conflict	1197	1	S → R in AAC46882. Ref.1
	Sequence conflict	1197	1	S → R in AAF25682. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (A) (C) (dNOS-1) [UniParc]. Last modified June 10, 2008. Version 3. Checksum: E19D8CBAEFAF76FC	FASTA	1,349	151,715
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 220 230 240 PKNQQQPRKM SQDYRSRAGS FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL 250 260 270 280 290 300 SCSKATCTSS IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 310 320 330 340 350 360 IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG MFEAICNHIK 370 380 390 400 410 420 YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ ADGKIIGDPM NVEFTEVCTK 430 440 450 460 470 480 LGWKSKGSEW DILPLVVSAN GHDPDYFDYP PELILEVPLT HPKFEWFSDL GLRWYALPAV 490 500 510 520 530 540 SSMLFDVGGI QFTATTFSGW YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD 550 560 570 580 590 600 KAVVEMNIAV LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI 610 620 630 640 650 660 TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI ARAVKFTSKL 670 680 690 700 710 720 FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ IYCMSDYDIS SIEHEALLIV 730 740 750 760 770 780 VASTFGNGDP PENGELFSQE LYAMRVQESS EHGLQDSSIG SSKSFMKASS RQEFMKLPLQ 790 800 810 820 830 840 QVKRIDRWDS LRGSTSDTFT EETFGPLSNV RFAVFALGSS AYPNFCAFGQ YVDNILGELG 850 860 870 880 890 900 GERLLRVAYG DEMCGQEQSF RKWAPEVFKL ACETFCLDPE ESLSDASLAL QNDSLTVNTV 910 920 930 940 950 960 RLVPSANKGS LDSSLSKYHN KKVHCCKAKA KPHNLTRLSE GAKTTMLLEI CAPGLEYEPG 970 980 990 1000 1010 1020 DHVGIFPANR TELVDGLLNR LVGVDNPDEV LQLQLLKEKQ TSNGIFKCWE PHDKIPPDTL 1030 1040 1050 1060 1070 1080 RNLLARFFDL TTPPSRQLLT LLAGFCEDTA DKERLELLVN DSSAYEDWRH WRLPHLLDVL 1090 1100 1110 1120 1130 1140 EEFPSCRPPA PLLLAQLTPL QPRFYSISSS PRRVSDEIHL TVAIVKYRCE DGQGDERYGV 1150 1160 1170 1180 1190 1200 CSNYLSGLRA DDELFMFVRS ALGFHLPSDR SRPIILIGPG TGIAPFRSFW QEFQVLSDLD 1210 1220 1230 1240 1250 1260 PTAKLPKMWL FFGCRNRDVD LYAEEKAELQ KDQILDRVFL ALSREQAIPK TYVQDLIEQE 1270 1280 1290 1300 1310 1320 FDSLYQLIVQ ERGHIYVCGD VTMAEHVYQT IRKCIAGKEQ KSEAEVETFL LTLRDESRYH 1330 1340 EDIFGITLRT AEIHTKSRAT ARIRMASQP
	Isoform 2 (dNOS-2) (E). Checksum: AD7AA548C03AE48F	FASTA	1,244	139,754
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 220 230 240 PKNQQQPRKM SQDYRSRAGS FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL 250 260 270 280 290 300 SCSKATCTSS IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 310 320 330 340 350 360 IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG MFEAICNHIK 370 380 390 400 410 420 YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ ADGKIIGDPM NVEFTETVAL 430 440 450 460 470 480 KMQLDTRTPT SLWKDKAVVE MNIAVLHSYQ SRNVTIVDHH TASESFMKHF ENESKLRNGC 490 500 510 520 530 540 PADWIWIVPP LSGSITPVFH QEMALYYLKP SFEYQDPAWR THVWKKGRGE SKGKKPRRKF 550 560 570 580 590 600 NFKQIARAVK FTSKLFGRAL SKRIKATVLY ATETGKSEQY AKQLCELLGH AFNAQIYCMS 610 620 630 640 650 660 DYDISSIEHE ALLIVVASTF GNGDPPENGE LFSQELYAMR VQESSEHGLQ DSSIGSSKSF 670 680 690 700 710 720 MKASSRQEFM KLPLQQVKRI DRWDSLRGST SDTFTEETFG PLSNVRFAVF ALGSSAYPNF 730 740 750 760 770 780 CAFGQYVDNI LGELGGERLL RVAYGDEMCG QEQSFRKWAP EVFKLACETF CLDPEESLSD 790 800 810 820 830 840 ASLALQNDSL TVNTVRLVPS ANKGSLDSSL SKYHNKKVHC CKAKAKPHNL TRLSEGAKTT 850 860 870 880 890 900 MLLEICAPGL EYEPGDHVGI FPANRTELVD GLLNRLVGVD NPDEVLQLQL LKEKQTSNGI 910 920 930 940 950 960 FKCWEPHDKI PPDTLRNLLA RFFDLTTPPS RQLLTLLAGF CEDTADKERL ELLVNDSSAY 970 980 990 1000 1010 1020 EDWRHWRLPH LLDVLEEFPS CRPPAPLLLA QLTPLQPRFY SISSSPRRVS DEIHLTVAIV 1030 1040 1050 1060 1070 1080 KYRCEDGQGD ERYGVCSNYL SGLRADDELF MFVRSALGFH LPSDRSRPII LIGPGTGIAP 1090 1100 1110 1120 1130 1140 FRSFWQEFQV LSDLDPTAKL PKMWLFFGCR NRDVDLYAEE KAELQKDQIL DRVFLALSRE 1150 1160 1170 1180 1190 1200 QAIPKTYVQD LIEQEFDSLY QLIVQERGHI YVCGDVTMAE HVYQTIRKCI AGKEQKSEAE 1210 1220 1230 1240 VETFLLTLRD ESRYHEDIFG ITLRTAEIHT KSRATARIRM ASQP
	Isoform 3 (dNOS-3). Checksum: AA970488F5BFBF76	FASTA	214	22,460
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 PKNQQQPRKM SQDYRSRAGS RFFPARRPPV RAAL
	Isoform 4 (B) (dNOS-4) (F) (J). Checksum: 1ABA7993149272D0	FASTA	756	84,261
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 220 230 240 PKNQQQPRKM SQDYRSRAGS FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL 250 260 270 280 290 300 SCSKATCTSS IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 310 320 330 340 350 360 IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG MFEAICNHIK 370 380 390 400 410 420 YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ ADGKIIGDPM NVEFTEVCTK 430 440 450 460 470 480 LGWKSKGSEW DILPLVVSAN GHDPDYFDYP PELILEVPLT HPKFEWFSDL GLRWYALPAV 490 500 510 520 530 540 SSMLFDVGGI QFTATTFSGW YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD 550 560 570 580 590 600 KAVVEMNIAV LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI 610 620 630 640 650 660 TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI ARAVKFTSKL 670 680 690 700 710 720 FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ IYCMSDYDIS SIEHEALLIV 730 740 750 VASTFGNGDP PENGEVSTPP RKDHTELING LGPAAF
	Isoform 5 (dNOS-5) (H). Checksum: EEF32F5F9BB8091E	FASTA	704	78,690
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 220 230 240 PKNQQQPRKM SQDYRSRAGS FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL 250 260 270 280 290 300 SCSKATCTSS IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 310 320 330 340 350 360 IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG MFEAICNHIK 370 380 390 400 410 420 YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ ADGKIIGDPM NVEFTEVCTK 430 440 450 460 470 480 LGWKSKGSEW DILPLVVSAN GHDPDYFDYP PELILEVPLT HPKFEWFSDL GLRWYALPAV 490 500 510 520 530 540 SSMLFDVGGI QFTATTFSGW YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD 550 560 570 580 590 600 KAVVEMNIAV LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI 610 620 630 640 650 660 TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI ARAVKFTSKL 670 680 690 700 FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ VSLT
	Isoform 6 (dNOS-6) (I). Checksum: 8449B48E111D7132	FASTA	746	83,144
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 220 230 240 PKNQQQPRKM SQDYRSRAGS FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL 250 260 270 280 290 300 SCSKATCTSS IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 310 320 330 340 350 360 IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG MFEAICNHIK 370 380 390 400 410 420 YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ ADGKIIGDPM NVEFTEVCTK 430 440 450 460 470 480 LGWKSKGSEW DILPLVVSAN GHDPDYFDYP PELILEVPLT HPKFEWFSDL GLRWYALPAV 490 500 510 520 530 540 SSMLFDVGGI QFTATTFSGW YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD 550 560 570 580 590 600 KAVVEMNIAV LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI 610 620 630 640 650 660 TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI ARAVKFTSKL 670 680 690 700 710 720 FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ CPVGSVSSDP QDPVQLGLVV 730 740 VLVSSTCVCK KHLPAAWQNK ISCKHL
	Isoform 10 (dNOS-10) (G). Checksum: 86C08065EBA6C062	FASTA	815	91,268
	10 20 30 40 50 60 MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ QNSRKIKTQA 70 80 90 100 110 120 TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG GLPSSSGTPL RHHKRASIST 130 140 150 160 170 180 ASPPIRERRG TNTSIVVELD GSGSGSGSGG GGVGVGQGAG CPPSGSCTAS GKSSRELSPS 190 200 210 220 230 240 PKNQQQPRKM SQDYRSRAGS FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL 250 260 270 280 290 300 SCSKATCTSS IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 310 320 330 340 350 360 IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG MFEAICNHIK 370 380 390 400 410 420 YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ ADGKIIGDPM NVEFTEVCTK 430 440 450 460 470 480 LGWKSKGSEW DILPLVVSAN GHDPDYFDYP PELILEVPLT HPKFEWFSDL GLRWYALPAV 490 500 510 520 530 540 SSMLFDVGGI QFTATTFSGW YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD 550 560 570 580 590 600 KAVVEMNIAV LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI 610 620 630 640 650 660 TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI ARAVKFTSKL 670 680 690 700 710 720 FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ IYCMSDYDIS SIEHEALLIV 730 740 750 760 770 780 VASTFGNGDP PENGELFSQE LYAMRVQESS EHGLQDSRKC IAGKEQKSEA EVETFLLTLR 790 800 810 DESRYHEDIF GITLRTAEIH TKSRATARIR MASQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biochemical characterization of dNOS: a Drosophila Ca2+/calmodulin-dependent nitric oxide synthase." Regulski M., Tully T. Proc. Natl. Acad. Sci. U.S.A. 92:9072-9076(1995) [PubMed: 7568075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, ENZYME REGULATION. Tissue: Head.
[2]	"The Drosophila nitric-oxide synthase gene (dNOS) encodes a family of proteins that can modulate NOS activity by acting as dominant negative regulators." Stasiv Y., Regulski M., Kuzin B., Tully T., Enikolopov G. J. Biol. Chem. 276:42241-42251(2001) [PubMed: 11526108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE. Tissue: Embryo and Larva.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Berkeley. Tissue: Embryo.
[6]	"Characterization of Drosophila nitric oxide synthase: a biochemical study." Sengupta R., Sahoo R., Mukherjee S., Regulski M., Tully T., Stuehr D.J., Ghosh S. Biochem. Biophys. Res. Commun. 306:590-597(2003) [PubMed: 12804606] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U25117 mRNA. Translation: AAC46882.1.
AF215700 , AF215691, AF215692, AF215693, AF215694, AF215695, AF215696, AF215697, AF215698, AF215699 Genomic DNA. Translation: AAF25682.1.
AE014134 Genomic DNA. Translation: AAF53014.1.
AE014134 Genomic DNA. Translation: AAZ66450.1.
AE014134 Genomic DNA. Translation: AAZ66451.1.
AE014134 Genomic DNA. Translation: AAZ66452.1.
AE014134 Genomic DNA. Translation: AAZ66453.1.
AE014134 Genomic DNA. Translation: AAZ66454.1.
AE014134 Genomic DNA. Translation: AAZ66455.1.
AE014134 Genomic DNA. Translation: AAZ66456.1.
AE014134 Genomic DNA. Translation: AAZ66457.1.
BT010016 mRNA. Translation: AAQ22485.1.

PIR

T13254.

RefSeq

NP_001027238.1.
NP_001027239.1.
NP_001027240.1.
NP_001027241.1.
NP_001027243.1.
NP_001027244.1.
NP_001027245.1.
NP_001027246.1.
NP_523541.2.

UniGene

Dm.2707

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I86	model	-	@	217-629	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q27571. 2 interactions.

Genome annotation databases

Ensembl

FBgn0011676. Drosophila melanogaster. [Contig view]

GeneID

34495.

KEGG

dme:Dmel_CG6713.

Organism-specific databases

FlyBase

FBgn0011676. Nos.

Phylogenomic databases

OMA

Q27571. KPSFEYQ.

Enzyme and pathway databases

BRENDA

1.14.13.39. 48.

Gene expression databases

ArrayExpress

Q27571.

GermOnline

CG6713. Drosophila melanogaster.

Family and domain databases

InterPro

IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR012144. Nitric-oxide_synthase.
IPR004030. NO_synthase_oxygenase_reg.
IPR001433. OxRdtase_FAD/NAD_bd.
[Graphical view]

Gene3D

G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.

Pfam

PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]

PIRSF

PIRSF000333. NOS. 1 hit.

PRINTS

PR00369. FLAVODOXIN.
PR00371. FPNCR.

PROSITE

PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

788761.

Entry information

Entry name

NOS_DROME

Accession

Primary (citable) accession number: Q27571
Secondary accession number(s): A4V0K9 , A4V0L0, Q4ABG3, Q4ABG4, Q4ABG5, Q4ABG6, Q4ABG7, Q7YU33, Q9U096, Q9VKP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 10, 2008
Last modified:	June 16, 2009
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project