Q27564 (KITH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase 1
Short name=TK1
EC=2.7.1.21

Alternative name(s):
Calmodulin-binding protein ThyB

Gene names

Name:	thyB
ORF Names:	DDB_G0289179

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. Ref.2
Subunit structure	Interacts with calmodulin in the presence of Ca2+. Ref.1
Developmental stage	Expression levels decrease throughout development. Ref.5 Ref.6
Sequence similarities	Belongs to the thymidine kinase family.
Biophysicochemical properties	Kinetic parameters: KM=5.1 µM for thymidine Ref.2

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Calmodulin-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from sequence or structural similarity Ref.1. Source: dictyBase calmodulin-dependent protein kinase activity Inferred from direct assay Ref.1. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from direct assay Ref.1Ref.2. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Thymidine kinase 1		PRO_0000293627
Regions
	Nucleotide binding	15 – 22	8	ATP Probable
	Nucleotide binding	47 – 49	3	ATP By similarity
	Nucleotide binding	91 – 94	4	ATP By similarity
	Region	166 – 170	5	Substrate binding By similarity
Sites
	Active site	92	1	Proton acceptor Potential
	Metal binding	147	1	Zinc By similarity
	Metal binding	150	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	182	1	Zinc By similarity
	Binding site	122	1	Substrate; via amide nitrogen By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27564 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CF96E644406C0772	FASTA	227	25,494
	10 20 30 40 50 60 MIVTQIAGKI QVIFGPMFSG KTTELIRRIK RFNFANKKCL LIKYSKDTRY NDNIDKSFLV 70 80 90 100 110 120 THDKQNYQAF PCSILEDVKE QAQNYDVIGI DEGQFFPDVV QFSEDLANQG KTVIIAALDG 130 140 150 160 170 180 TFQRKPFQSV IDLVSKAEYI TKLTAVCMVC YNEAAFSKRI VESDDIELIG GIDKYISVCR 190 200 210 220 GCYNSDQNEG NSTKPSKTAR HSHSQSAPSV APLAVNINPD DHLNNDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein." O'Day D.H., Chatterjee-Chakraborty M., Wagler S., Myre M.A. Biochem. Biophys. Res. Commun. 331:1494-1502(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
[2]	"Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases." Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J. J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: AX4.
[3]	"Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome." Kuspa A., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AX4.
[4]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[5]	"A transcriptional profile of multicellular development in Dictyostelium discoideum." Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G. Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[6]	"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum." Iranfar N., Fuller D., Loomis W.F. Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY192984 mRNA. Translation: AAO64434.1. AF510846 mRNA. Translation: AAM44288.1. U61990 mRNA. Translation: AAB03673.1. AAFI02000131 Genomic DNA. Translation: EAL62823.1.
RefSeq	XP_636351.1. XM_631259.1.
3D structure databases
HSSP	HSSP built from PDB template 1W4R based on UniProtKB P04183.
ProteinModelPortal	Q27564.
SMR	Q27564. Positions 7-184.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191436; DDB0191436; DDB_G0289179.
GeneID	8627024.
KEGG	ddi:DDB_G0289179.
Organism-specific databases
dictyBase	DDB_G0289179. thyB.
Phylogenomic databases
eggNOG	COG1435.
KO	K00857.
OMA	KVHAICV.
ProtClustDB	PTZ00293.
Enzyme and pathway databases
BRENDA	2.7.1.21. 1939.
SABIO-RK	Q27564.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_DICDI

Accession

Primary (citable) accession number: Q27564
Secondary accession number(s): Q54HT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)