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Q27564

- KITH_DICDI

UniProt

Q27564 - KITH_DICDI

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Protein
Thymidine kinase 1
Gene
thyB, DDB_G0289179
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

Kineticsi

  1. KM=5.1 µM for thymidine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Proton acceptor Reviewed prediction
Binding sitei122 – 1221Substrate; via amide nitrogen By similarity
Metal bindingi147 – 1471Zinc By similarity
Metal bindingi150 – 1501Zinc By similarity
Binding sitei175 – 1751Substrate By similarity
Metal bindingi179 – 1791Zinc By similarity
Metal bindingi182 – 1821Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATP Inferred
Nucleotide bindingi47 – 493ATP By similarity
Nucleotide bindingi91 – 944ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: dictyBase
  2. calmodulin binding Source: dictyBase
  3. calmodulin-dependent protein kinase activity Source: dictyBase
  4. metal ion binding Source: UniProtKB-KW
  5. protein homodimerization activity Source: dictyBase
  6. thymidine kinase activity Source: dictyBase
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
  3. nucleobase-containing compound metabolic process Source: dictyBase
  4. protein phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21. 1939.
SABIO-RKQ27564.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase 1 (EC:2.7.1.21)
Short name:
TK1
Alternative name(s):
Calmodulin-binding protein ThyB
Gene namesi
Name:thyB
ORF Names:DDB_G0289179
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289179. thyB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Thymidine kinase 1
PRO_0000293627Add
BLAST

Expressioni

Developmental stagei

Expression levels decrease throughout development.2 Publications

Interactioni

Subunit structurei

Interacts with calmodulin in the presence of Ca2+.1 Publication

Protein-protein interaction databases

MINTiMINT-8411759.
STRINGi44689.DDB_0191436.

Structurei

3D structure databases

ProteinModelPortaliQ27564.
SMRiQ27564. Positions 7-184.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 1705Substrate binding By similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

eggNOGiCOG1435.
KOiK00857.
OMAiQAICLSC.
PhylomeDBiQ27564.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27564-1 [UniParc]FASTAAdd to Basket

« Hide

MIVTQIAGKI QVIFGPMFSG KTTELIRRIK RFNFANKKCL LIKYSKDTRY    50
NDNIDKSFLV THDKQNYQAF PCSILEDVKE QAQNYDVIGI DEGQFFPDVV 100
QFSEDLANQG KTVIIAALDG TFQRKPFQSV IDLVSKAEYI TKLTAVCMVC 150
YNEAAFSKRI VESDDIELIG GIDKYISVCR GCYNSDQNEG NSTKPSKTAR 200
HSHSQSAPSV APLAVNINPD DHLNNDY 227
Length:227
Mass (Da):25,494
Last modified:November 1, 1996 - v1
Checksum:iCF96E644406C0772
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY192984 mRNA. Translation: AAO64434.1.
AF510846 mRNA. Translation: AAM44288.1.
U61990 mRNA. Translation: AAB03673.1.
AAFI02000131 Genomic DNA. Translation: EAL62823.1.
RefSeqiXP_636351.1. XM_631259.1.

Genome annotation databases

EnsemblProtistsiDDB0191436; DDB0191436; DDB_G0289179.
GeneIDi8627024.
KEGGiddi:DDB_G0289179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY192984 mRNA. Translation: AAO64434.1 .
AF510846 mRNA. Translation: AAM44288.1 .
U61990 mRNA. Translation: AAB03673.1 .
AAFI02000131 Genomic DNA. Translation: EAL62823.1 .
RefSeqi XP_636351.1. XM_631259.1.

3D structure databases

ProteinModelPortali Q27564.
SMRi Q27564. Positions 7-184.
ModBasei Search...

Protein-protein interaction databases

MINTi MINT-8411759.
STRINGi 44689.DDB_0191436.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191436 ; DDB0191436 ; DDB_G0289179 .
GeneIDi 8627024.
KEGGi ddi:DDB_G0289179.

Organism-specific databases

dictyBasei DDB_G0289179. thyB.

Phylogenomic databases

eggNOGi COG1435.
KOi K00857.
OMAi QAICLSC.
PhylomeDBi Q27564.

Enzyme and pathway databases

BRENDAi 2.7.1.21. 1939.
SABIO-RK Q27564.

Miscellaneous databases

PROi Q27564.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein."
    O'Day D.H., Chatterjee-Chakraborty M., Wagler S., Myre M.A.
    Biochem. Biophys. Res. Commun. 331:1494-1502(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
  2. "Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
    Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
    J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: AX4.
  3. "Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome."
    Kuspa A., Loomis W.F.
    Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AX4.
  4. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  5. "A transcriptional profile of multicellular development in Dictyostelium discoideum."
    Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
    Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
    Iranfar N., Fuller D., Loomis W.F.
    Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiKITH_DICDI
AccessioniPrimary (citable) accession number: Q27564
Secondary accession number(s): Q54HT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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