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Protein

Thymidine kinase 1

Gene

thyB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

Kineticsi

  1. KM=5.1 µM for thymidine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Proton acceptorSequence Analysis
    Binding sitei122 – 1221Substrate; via amide nitrogenBy similarity
    Metal bindingi147 – 1471ZincBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Binding sitei175 – 1751SubstrateBy similarity
    Metal bindingi179 – 1791ZincBy similarity
    Metal bindingi182 – 1821ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228ATPSequence AnalysisCurated
    Nucleotide bindingi47 – 493ATPBy similarity
    Nucleotide bindingi91 – 944ATPBy similarity

    GO - Molecular functioni

    • ATP binding Source: dictyBase
    • calmodulin binding Source: dictyBase
    • calmodulin-dependent protein kinase activity Source: dictyBase
    • metal ion binding Source: UniProtKB-KW
    • protein homodimerization activity Source: dictyBase
    • thymidine kinase activity Source: dictyBase

    GO - Biological processi

    • deoxyribonucleoside monophosphate biosynthetic process Source: GOC
    • DNA biosynthetic process Source: UniProtKB-KW
    • nucleobase-containing compound metabolic process Source: dictyBase
    • protein phosphorylation Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.1.21. 1939.
    ReactomeiREACT_307855. Pyrimidine salvage reactions.
    SABIO-RKQ27564.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase 1 (EC:2.7.1.21)
    Short name:
    TK1
    Alternative name(s):
    Calmodulin-binding protein ThyB
    Gene namesi
    Name:thyBImported
    ORF Names:DDB_G0289179
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0289179. thyB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Thymidine kinase 1PRO_0000293627Add
    BLAST

    Expressioni

    Developmental stagei

    Expression levels decrease throughout development.2 Publications

    Interactioni

    Subunit structurei

    Interacts with calmodulin in the presence of Ca2+.1 Publication

    Protein-protein interaction databases

    MINTiMINT-8411759.
    STRINGi44689.DDB0191436.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27564.
    SMRiQ27564. Positions 7-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1705Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG1435.
    InParanoidiQ27564.
    KOiK00857.
    OMAiVTKVHAI.
    PhylomeDBiQ27564.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27564-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIVTQIAGKI QVIFGPMFSG KTTELIRRIK RFNFANKKCL LIKYSKDTRY
    60 70 80 90 100
    NDNIDKSFLV THDKQNYQAF PCSILEDVKE QAQNYDVIGI DEGQFFPDVV
    110 120 130 140 150
    QFSEDLANQG KTVIIAALDG TFQRKPFQSV IDLVSKAEYI TKLTAVCMVC
    160 170 180 190 200
    YNEAAFSKRI VESDDIELIG GIDKYISVCR GCYNSDQNEG NSTKPSKTAR
    210 220
    HSHSQSAPSV APLAVNINPD DHLNNDY
    Length:227
    Mass (Da):25,494
    Last modified:November 1, 1996 - v1
    Checksum:iCF96E644406C0772
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY192984 mRNA. Translation: AAO64434.1.
    AF510846 mRNA. Translation: AAM44288.1.
    U61990 mRNA. Translation: AAB03673.1.
    AAFI02000131 Genomic DNA. Translation: EAL62823.1.
    RefSeqiXP_636351.1. XM_631259.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191436; DDB0191436; DDB_G0289179.
    GeneIDi8627024.
    KEGGiddi:DDB_G0289179.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY192984 mRNA. Translation: AAO64434.1.
    AF510846 mRNA. Translation: AAM44288.1.
    U61990 mRNA. Translation: AAB03673.1.
    AAFI02000131 Genomic DNA. Translation: EAL62823.1.
    RefSeqiXP_636351.1. XM_631259.1.

    3D structure databases

    ProteinModelPortaliQ27564.
    SMRiQ27564. Positions 7-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    MINTiMINT-8411759.
    STRINGi44689.DDB0191436.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsiDDB0191436; DDB0191436; DDB_G0289179.
    GeneIDi8627024.
    KEGGiddi:DDB_G0289179.

    Organism-specific databases

    dictyBaseiDDB_G0289179. thyB.

    Phylogenomic databases

    eggNOGiCOG1435.
    InParanoidiQ27564.
    KOiK00857.
    OMAiVTKVHAI.
    PhylomeDBiQ27564.

    Enzyme and pathway databases

    BRENDAi2.7.1.21. 1939.
    ReactomeiREACT_307855. Pyrimidine salvage reactions.
    SABIO-RKQ27564.

    Miscellaneous databases

    PROiQ27564.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein."
      O'Day D.H., Chatterjee-Chakraborty M., Wagler S., Myre M.A.
      Biochem. Biophys. Res. Commun. 331:1494-1502(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
    2. "Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
      Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
      J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: AX41 Publication.
    3. "Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome."
      Kuspa A., Loomis W.F.
      Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: AX4.
    4. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4Imported.
    5. "A transcriptional profile of multicellular development in Dictyostelium discoideum."
      Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
      Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    6. "Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
      Iranfar N., Fuller D., Loomis W.F.
      Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiKITH_DICDI
    AccessioniPrimary (citable) accession number: Q27564
    Secondary accession number(s): Q54HT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: November 1, 1996
    Last modified: July 22, 2015
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.