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Q27564 (KITH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase 1

Short name=TK1
EC=2.7.1.21
Alternative name(s):
Calmodulin-binding protein ThyB
Gene names
Name:thyB
ORF Names:DDB_G0289179
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate. Ref.2

Subunit structure

Interacts with calmodulin in the presence of Ca2+. Ref.1

Developmental stage

Expression levels decrease throughout development. Ref.5 Ref.6

Sequence similarities

Belongs to the thymidine kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=5.1 µM for thymidine Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Thymidine kinase 1
PRO_0000293627

Regions

Nucleotide binding15 – 228ATP Probable
Nucleotide binding47 – 493ATP By similarity
Nucleotide binding91 – 944ATP By similarity
Region166 – 1705Substrate binding By similarity

Sites

Active site921Proton acceptor Potential
Metal binding1471Zinc By similarity
Metal binding1501Zinc By similarity
Metal binding1791Zinc By similarity
Metal binding1821Zinc By similarity
Binding site1221Substrate; via amide nitrogen By similarity
Binding site1751Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27564 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CF96E644406C0772

FASTA22725,494
        10         20         30         40         50         60 
MIVTQIAGKI QVIFGPMFSG KTTELIRRIK RFNFANKKCL LIKYSKDTRY NDNIDKSFLV 

        70         80         90        100        110        120 
THDKQNYQAF PCSILEDVKE QAQNYDVIGI DEGQFFPDVV QFSEDLANQG KTVIIAALDG 

       130        140        150        160        170        180 
TFQRKPFQSV IDLVSKAEYI TKLTAVCMVC YNEAAFSKRI VESDDIELIG GIDKYISVCR 

       190        200        210        220 
GCYNSDQNEG NSTKPSKTAR HSHSQSAPSV APLAVNINPD DHLNNDY 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein."
O'Day D.H., Chatterjee-Chakraborty M., Wagler S., Myre M.A.
Biochem. Biophys. Res. Commun. 331:1494-1502(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
[2]"Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: AX4.
[3]"Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome."
Kuspa A., Loomis W.F.
Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AX4.
[4]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[5]"A transcriptional profile of multicellular development in Dictyostelium discoideum."
Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
Iranfar N., Fuller D., Loomis W.F.
Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY192984 mRNA. Translation: AAO64434.1.
AF510846 mRNA. Translation: AAM44288.1.
U61990 mRNA. Translation: AAB03673.1.
AAFI02000131 Genomic DNA. Translation: EAL62823.1.
RefSeqXP_636351.1. XM_631259.1.

3D structure databases

ProteinModelPortalQ27564.
SMRQ27564. Positions 7-184.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8411759.
STRING44689.DDB_0191436.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191436; DDB0191436; DDB_G0289179.
GeneID8627024.
KEGGddi:DDB_G0289179.

Organism-specific databases

dictyBaseDDB_G0289179. thyB.

Phylogenomic databases

eggNOGCOG1435.
OMAQAICLSC.
PhylomeDBQ27564.

Enzyme and pathway databases

BRENDA2.7.1.21. 1939.
SABIO-RKQ27564.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ27564.

Entry information

Entry nameKITH_DICDI
AccessionPrimary (citable) accession number: Q27564
Secondary accession number(s): Q54HT8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase