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Protein

Proteasome subunit alpha type-3

Gene

psmA3

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: dictyBase
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-DDI-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DDI-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DDI-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DDI-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DDI-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DDI-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-DDI-382556. ABC-family proteins mediated transport.
R-DDI-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DDI-5632684. Hedgehog 'on' state.
R-DDI-5658442. Regulation of RAS by GAPs.
R-DDI-5689603. UCH proteinases.
R-DDI-5689880. Ub-specific processing proteases.
R-DDI-68949. Orc1 removal from chromatin.
R-DDI-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DDI-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DDI-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DDI-8948751. Regulation of PTEN stability and activity.
R-DDI-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-3 (EC:3.4.25.1)
Gene namesi
Name:psmA3
Synonyms:prtD
ORF Names:DDB_G0267408
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267408. psmA3.

Subcellular locationi

Q27563:

GO - Cellular componenti

  • cytoplasm Source: dictyBase
  • nucleus Source: dictyBase
  • proteasome core complex, alpha-subunit complex Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001240961 – 248Proteasome subunit alpha type-3Add BLAST248

Proteomic databases

PaxDbiQ27563.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191264.

Structurei

3D structure databases

ProteinModelPortaliQ27563.
SMRiQ27563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0184. Eukaryota.
ENOG410XP01. LUCA.
InParanoidiQ27563.
KOiK02727.
OMAiFELEMTW.
PhylomeDBiQ27563.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR023332. Proteasome_alpha-type.
IPR035150. Proteasome_alpha3.
IPR000426. Proteasome_asu_N.
IPR001353. Proteasome_sua/b.
PANTHERiPTHR11599:SF103. PTHR11599:SF103. 1 hit.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
SMARTiView protein in SMART
SM00948. Proteasome_A_N. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVGSGYDL YVSTYSPDGK LFQVDYANKA VENSGTLVAI KAKDGVVLGV
60 70 80 90 100
EKLVPSKMLC SGSNRRVHHI DTHVGVAIAG FLADARQLIT RARSEAKNYK
110 120 130 140 150
STYGQPIPIK VLSQRIASYN HMHTLYGSVR PFGCSIAITG IDQYGPQLFL
160 170 180 190 200
VEPSGSCVGY FGASLGKGKQ AAKNELEKIK FSEMTCREAI KEVSRIIYSV
210 220 230 240
HDEVKDKDFE LELGWISTET NNVHQIVPKE LHDEAEAYAK QSLEDANM
Length:248
Mass (Da):27,238
Last modified:December 4, 2007 - v2
Checksum:iF6BAAE2666948CBB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178 – 180KIK → EIR in AAB03671 (PubMed:8643615).Curated3
Sequence conflicti221N → D in AAB03671 (PubMed:8643615).Curated1
Sequence conflicti235A → G in AAB03671 (PubMed:8643615).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61988 mRNA. Translation: AAB03671.1.
AAFI02000003 Genomic DNA. Translation: EAL73156.1.
RefSeqiXP_647103.1. XM_642011.1.

Genome annotation databases

EnsemblProtistsiEAL73156; EAL73156; DDB_G0267408.
GeneIDi8615907.
KEGGiddi:DDB_G0267408.

Similar proteinsi

Entry informationi

Entry nameiPSA3_DICDI
AccessioniPrimary (citable) accession number: Q27563
Secondary accession number(s): Q55GT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 4, 2007
Last modified: October 25, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families