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Q27563 (PSA3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha type-3

EC=3.4.25.1
Gene names
Name:psmA3
Synonyms:prtD
ORF Names:DDB_G0267408
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Proteasome subunit alpha type-3
PRO_0000124096

Experimental info

Sequence conflict178 – 1803KIK → EIR in AAB03671. Ref.1
Sequence conflict2211N → D in AAB03671. Ref.1
Sequence conflict2351A → G in AAB03671. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q27563 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: F6BAAE2666948CBB

FASTA24827,238
        10         20         30         40         50         60 
MSSVGSGYDL YVSTYSPDGK LFQVDYANKA VENSGTLVAI KAKDGVVLGV EKLVPSKMLC 

        70         80         90        100        110        120 
SGSNRRVHHI DTHVGVAIAG FLADARQLIT RARSEAKNYK STYGQPIPIK VLSQRIASYN 

       130        140        150        160        170        180 
HMHTLYGSVR PFGCSIAITG IDQYGPQLFL VEPSGSCVGY FGASLGKGKQ AAKNELEKIK 

       190        200        210        220        230        240 
FSEMTCREAI KEVSRIIYSV HDEVKDKDFE LELGWISTET NNVHQIVPKE LHDEAEAYAK 


QSLEDANM 

« Hide

References

[1]"Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome."
Kuspa A., Loomis W.F.
Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U61988 mRNA. Translation: AAB03671.1.
AAFI02000003 Genomic DNA. Translation: EAL73156.1.
RefSeqXP_647103.1. XM_642011.1.

3D structure databases

ProteinModelPortalQ27563.
SMRQ27563. Positions 2-245.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191264.

Proteomic databases

PRIDEQ27563.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191264; DDB0191264; DDB_G0267408.
GeneID8615907.
KEGGddi:DDB_G0267408.

Organism-specific databases

dictyBaseDDB_G0267408. psmA3.

Phylogenomic databases

eggNOGCOG0638.
OMAVPDGRHF.
PhylomeDBQ27563.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ27563.

Entry information

Entry namePSA3_DICDI
AccessionPrimary (citable) accession number: Q27563
Secondary accession number(s): Q55GT1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase