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Q27563

- PSA3_DICDI

UniProt

Q27563 - PSA3_DICDI

Protein

Proteasome subunit alpha type-3

Gene

psmA3

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis Source: dictyBase
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_204617. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_210566. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_212010. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_214682. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_217211. ER-Phagosome pathway.
    REACT_217636. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_221036. Separation of Sister Chromatids.
    REACT_225025. APC/C:Cdc20 mediated degradation of Securin.
    REACT_226311. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_226712. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha type-3 (EC:3.4.25.1)
    Gene namesi
    Name:psmA3
    Synonyms:prtD
    ORF Names:DDB_G0267408
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0267408. psmA3.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. nucleus Source: dictyBase
    3. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Proteasome subunit alpha type-3PRO_0000124096Add
    BLAST

    Proteomic databases

    PRIDEiQ27563.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0191264.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27563.
    SMRiQ27563. Positions 2-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    KOiK02727.
    OMAiVPDGRHF.
    PhylomeDBiQ27563.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27563-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSVGSGYDL YVSTYSPDGK LFQVDYANKA VENSGTLVAI KAKDGVVLGV    50
    EKLVPSKMLC SGSNRRVHHI DTHVGVAIAG FLADARQLIT RARSEAKNYK 100
    STYGQPIPIK VLSQRIASYN HMHTLYGSVR PFGCSIAITG IDQYGPQLFL 150
    VEPSGSCVGY FGASLGKGKQ AAKNELEKIK FSEMTCREAI KEVSRIIYSV 200
    HDEVKDKDFE LELGWISTET NNVHQIVPKE LHDEAEAYAK QSLEDANM 248
    Length:248
    Mass (Da):27,238
    Last modified:December 4, 2007 - v2
    Checksum:iF6BAAE2666948CBB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti178 – 1803KIK → EIR in AAB03671. (PubMed:8643615)Curated
    Sequence conflicti221 – 2211N → D in AAB03671. (PubMed:8643615)Curated
    Sequence conflicti235 – 2351A → G in AAB03671. (PubMed:8643615)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U61988 mRNA. Translation: AAB03671.1.
    AAFI02000003 Genomic DNA. Translation: EAL73156.1.
    RefSeqiXP_647103.1. XM_642011.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191264; DDB0191264; DDB_G0267408.
    GeneIDi8615907.
    KEGGiddi:DDB_G0267408.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U61988 mRNA. Translation: AAB03671.1 .
    AAFI02000003 Genomic DNA. Translation: EAL73156.1 .
    RefSeqi XP_647103.1. XM_642011.1.

    3D structure databases

    ProteinModelPortali Q27563.
    SMRi Q27563. Positions 2-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191264.

    Proteomic databases

    PRIDEi Q27563.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191264 ; DDB0191264 ; DDB_G0267408 .
    GeneIDi 8615907.
    KEGGi ddi:DDB_G0267408.

    Organism-specific databases

    dictyBasei DDB_G0267408. psmA3.

    Phylogenomic databases

    eggNOGi COG0638.
    KOi K02727.
    OMAi VPDGRHF.
    PhylomeDBi Q27563.

    Enzyme and pathway databases

    Reactomei REACT_204617. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_210566. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_212010. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_214682. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_217211. ER-Phagosome pathway.
    REACT_217636. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_221036. Separation of Sister Chromatids.
    REACT_225025. APC/C:Cdc20 mediated degradation of Securin.
    REACT_226311. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_226712. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Miscellaneous databases

    PROi Q27563.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome."
      Kuspa A., Loomis W.F.
      Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPSA3_DICDI
    AccessioniPrimary (citable) accession number: Q27563
    Secondary accession number(s): Q55GT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3