Reviewed, UniProtKB/Swiss-Prot Q27562 (PSA1_DICDI)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-1
    EC=3.4.25.1
Alternative name(s):
    Proteasome subunit C2

Gene names

Name:	psmA1
Synonyms:	prtC
ORF Names:	DDB_G0282363

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Proteasome subunit alpha type-1		PRO_0000124067

Sequences

	Sequence		Length	Mass (Da)
	Q27562-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 42C717B37BDA0F3B	FASTA	248	27,988
	10 20 30 40 50 60 MYRNQYDSDI TTFSPSGRIH QIEYAMEAVK QGGAAVALKS KQFAVLVSLK RSSSELGSYQ 70 80 90 100 110 120 KKIFVVDDHV GIAISGLTAD ARMLCNYMRN ECSNYNYVYE SQMRVERLVT KVADKHQVHT 130 140 150 160 170 180 QRYGRRPYGV GLLVAGYDQL GAHIYQTCPS GNFYDYKSIS IGSRSQSAKT YLEKHFESFE 190 200 210 220 230 240 ECTLEQLITH GLRALRETIP NTDDTLNSKN VSIGIVGEGQ PFKIIEEADA QPYLNLLENE EPVEMQTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Developmental signal transduction pathways uncovered by genetic suppressors." Shaulsky G., Escalante R., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 93:15260-15265(1996) [PubMed: 8986798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U60168 Genomic DNA. Translation: AAB03506.1. AAFI02000047 Genomic DNA. Translation: EAL66041.1.
RefSeq	XP_640037.1.
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P40302.
ModBase	Search...
Protein-protein interaction databases
STRING	Q27562.
Protein family/group databases
MEROPS	T01.976.
Genome annotation databases
GeneID	3391327.
GenomeReviews	Gene locus psmA1 in contig CM000152_GR.
KEGG	ddi:DDB_0214956.
Organism-specific databases
dictyBase	DDB_G0282363. psmA1.
Phylogenomic databases
OMA	YAVEAMK.
Enzyme and pathway databases
BRENDA	3.4.25.1. 424.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA1_DICDI

Accession

Primary (citable) accession number: Q27562
Secondary accession number(s): Q54SK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)