ID   GPDA_DROAE              Reviewed;         350 AA.
AC   Q27556;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic;
DE            Short=GPDH-C;
DE            Short=GPD-C;
DE            EC=1.1.1.8;
GN   Name=Gpdh;
OS   Drosophila americana (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila.
OX   NCBI_TaxID=40366;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=96025618; PubMed=8522168; DOI=10.1007/BF01439583;
RA   Tominaga H., Narise S.;
RT   "Sequence evolution of the Gpdh gene in the Drosophila virilis species
RT   group.";
RL   Genetica 96:293-302(1995).
CC   -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(+) = glycerone
CC       phosphate + NADH.
CC   -!- PATHWAY: Phospholipid metabolism; alpha-glycerophosphate cycle.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; D50089; BAA20286.1; -; Genomic_DNA.
DR   FlyBase; FBgn0015636; Dame\Gpdh.
DR   BRENDA; 1.1.1.8; 304551.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase (NAD+) a...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006168; NAD-dep_Gly3P_DH.
DR   InterPro; IPR017751; NAD-dep_Gly3P_DH_euk.
DR   InterPro; IPR011128; NAD-dep_Gly3P_DH_N.
DR   InterPro; IPR006109; NAD_Gly3P_DH_C.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR11728; NAD_Gly3P_DH; 1.
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR   PRINTS; PR00077; GPDHDRGNASE.
DR   ProDom; PD001278; NAD_Gly3P_C; 1.
DR   TIGRFAMs; TIGR03376; glycerol3P_DH; 1.
DR   PROSITE; PS00957; NAD_G3PDH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    350       Glycerol-3-phosphate dehydrogenase
FT                                [NAD+], cytoplasmic.
FT                                /FTId=PRO_0000138072.
FT   NP_BIND      11     16       NAD (By similarity).
FT   REGION      270    271       Substrate binding (By similarity).
FT   ACT_SITE    206    206       Proton acceptor (By similarity).
FT   BINDING      98     98       NAD (By similarity).
FT   BINDING     121    121       NAD; via amide nitrogen (By similarity).
FT   BINDING     121    121       Substrate (By similarity).
FT   BINDING     155    155       NAD; via amide nitrogen (By similarity).
FT   BINDING     270    270       NAD (By similarity).
FT   BINDING     299    299       NAD (By similarity).
SQ   SEQUENCE   350 AA;  38323 MW;  E23908D262F6D660 CRC64;
     MAEKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEEMIDGKK LTEIINETHE
     NVKYLKGHKL PTNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI
     KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR
     DLFQANHFRV VVVEDAEAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF
     VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEDLEK EMLNGQKLQG
     PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC TNQLKPKDLI DCIRNHPEHM
//