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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

DHFR-TS

Organism
Cryptosporidium parvum
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11NADP; via amide nitrogen and carbonyl oxygenCombined sources1
Binding sitei19NADP; via carbonyl oxygenCombined sources1
Binding sitei24NADP; via amide nitrogen and carbonyl oxygenCombined sources1
Binding sitei92NADP; via carbonyl oxygenCombined sources1
Active sitei402UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 58NADPCombined sources3
Nucleotide bindingi75 – 78NADPCombined sources4
Nucleotide bindingi116 – 117NADPCombined sources2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotation, OxidoreductaseUniRule annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation
LigandNADPCombined sources, Nucleotide-bindingCombined sources

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
Gene namesi
Name:DHFR-TSImported
OrganismiCryptosporidium parvumImported
Taxonomic identifieri5807 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaCryptosporidiidaeCryptosporidium

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2366489.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QZFX-ray2.80A/B/C/D/E1-521[»]
1SEJX-ray2.87A/B/C/D/E1-521[»]
ProteinModelPortaliQ27552.
SMRiQ27552.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ27552.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 177DHFRInterPro annotationAdd BLAST173

Sequence similaritiesi

In the C-terminal section; belongs to the thymidylate synthase family.UniRule annotation
In the N-terminal section; belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG410K5TU. Eukaryota.
KOG0673. Eukaryota.
KOG1324. Eukaryota.
COG0207. LUCA.
COG0262. LUCA.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
3.40.430.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR024072. DHFR-like_dom_sf.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PANTHERiPTHR11548:SF7. PTHR11548:SF7. 1 hit.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKNVSIVV AASVLSSGIG INGQLPWSIS EDLKFFSKIT NNKCDSNKKN
60 70 80 90 100
ALIMGRKTWD SIGRRPLKNR IIVVISSSLP QDEADPNVVV FRNLEDSIEN
110 120 130 140 150
LMNDDSIENI FVCGGESIYR DALKDNFVDR IYLTRVALED IEFDTYFPEI
160 170 180 190 200
PETFLPVYMS QTFCTKNISY DFMIFEKQEK KTLQNCDPAR GQLKSIDDTV
210 220 230 240 250
DLLGEIFGIR KMGNRHKFPK EEIYNTPSIR FGREHYEFQY LDLLSRVLEN
260 270 280 290 300
GAYRENRTGI STYSIFGQMM RFDMRESFPL LTTKKVAIRS IFEELIWFIK
310 320 330 340 350
GDTNGNHLIE KKVYIWSGNG SKEYLERIGL GHREENDLGP IYGFQWRHYN
360 370 380 390 400
GEYKTMHDDY TGVGVDQLAK LIETLKNNPK DRRHILTAWN PSALSQMALP
410 420 430 440 450
PCHVLSQYYV TNDNCLSCNL YQRSCDLGLG SPFNIASYAI LTMMLAQVCG
460 470 480 490 500
YEPGELAIFI GDAHIYENHL TQLKEQLSRT PRPFPQLKFK RKVENIEDFK
510 520
WEDIELIGYY PYPTIKMDMA V
Length:521
Mass (Da):60,192
Last modified:November 1, 1996 - v1
Checksum:i4AFFEEA5C2A9B8E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41366 Genomic DNA. Translation: AAC47230.1.

Similar proteinsi

Entry informationi

Entry nameiQ27552_CRYPV
AccessioniPrimary (citable) accession number: Q27552
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources