Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q27546

- IUNH_CRIFA

UniProt

Q27546 - IUNH_CRIFA

Protein

Inosine-uridine preferring nucleoside hydrolase

Gene

IUNH

Organism
Crithidia fasciculata
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, but has a preference for inosine and uridine as substrates.

    Catalytic activityi

    A purine nucleoside + H2O = D-ribose + a purine base.

    Cofactori

    Calcium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi10 – 101Calcium
    Binding sitei14 – 141Substrate1 Publication
    Metal bindingi15 – 151Calcium
    Metal bindingi126 – 1261Calcium
    Binding sitei160 – 1601Substrate1 Publication
    Binding sitei166 – 1661Substrate1 Publication
    Binding sitei168 – 1681Substrate1 Publication
    Active sitei241 – 2411
    Metal bindingi242 – 2421Calcium

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. nucleotide metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-uridine preferring nucleoside hydrolase (EC:3.2.2.-)
    Short name:
    IU-NH
    Short name:
    IU-nucleoside hydrolase
    Alternative name(s):
    Non-specific nucleoside hydrolase
    Purine nucleosidase
    Gene namesi
    Name:IUNH
    OrganismiCrithidia fasciculata
    Taxonomic identifieri5656 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeCrithidia

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi241 – 2411H → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 315314Inosine-uridine preferring nucleoside hydrolasePRO_0000206810Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    Secondary structure

    1
    315
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Helixi13 – 2412
    Beta strandi28 – 358
    Beta strandi37 – 404
    Helixi42 – 5514
    Beta strandi63 – 653
    Beta strandi71 – 733
    Helixi79 – 824
    Beta strandi84 – 874
    Helixi105 – 11511
    Beta strandi121 – 1255
    Helixi130 – 1389
    Helixi142 – 1454
    Beta strandi148 – 1525
    Beta strandi160 – 1645
    Helixi167 – 1704
    Helixi173 – 1819
    Beta strandi182 – 1843
    Beta strandi186 – 1894
    Helixi191 – 1944
    Helixi201 – 2088
    Helixi213 – 23018
    Beta strandi236 – 2394
    Helixi242 – 2509
    Helixi252 – 2543
    Beta strandi255 – 2595
    Beta strandi262 – 2643
    Turni269 – 2735
    Beta strandi275 – 2773
    Beta strandi288 – 2958
    Helixi297 – 31115

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MASX-ray2.50A/B3-315[»]
    2MASX-ray2.30A/B/C/D3-315[»]
    ProteinModelPortaliQ27546.
    SMRiQ27546. Positions 3-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ27546.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IUNH family.Curated

    Family and domain databases

    Gene3Di3.90.245.10. 1 hit.
    InterProiIPR015910. I/U_nuclsd_hydro_CS.
    IPR023186. Inosine/uridine_hydrolase.
    IPR001910. Inosine/uridine_hydrolase_dom.
    [Graphical view]
    PANTHERiPTHR12304. PTHR12304. 1 hit.
    PfamiPF01156. IU_nuc_hydro. 1 hit.
    [Graphical view]
    SUPFAMiSSF53590. SSF53590. 1 hit.
    PROSITEiPS01247. IUNH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27546-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKKIILDCD PGLDDAVAIL LAHGNPEIEL LAITTVVGNQ TLAKVTRNAQ    50
    LVADIAGITG VPIAAGCDKP LVRKIMTAGH IHGESGMGTV AYPAEFKNKV 100
    DERHAVNLII DLVMSHEPKT ITLVPTGGLT NIAMAARLEP RIVDRVKEVV 150
    LMGGGYHEGN ATSVAEFNII IDPEAAHIVF NESWQVTMVG LDLTHQALAT 200
    PPILQRVKEV DTNPARFMLE IMDYYTKIYQ SNRYMAAAAV HDPCAVAYVI 250
    DPSVMTTERV PVDIELTGKL TLGMTVADFR NPRPEHCHTQ VAVKLDFEKF 300
    WGLVLDALER IGDPQ 315
    Length:315
    Mass (Da):34,326
    Last modified:January 23, 2007 - v3
    Checksum:iB88CF9DA41B53F43
    GO

    Mass spectrometryi

    Molecular mass is 34194±4 Da from positions 2 - 315. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43371 Genomic DNA. Translation: AAC47119.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43371 Genomic DNA. Translation: AAC47119.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MAS X-ray 2.50 A/B 3-315 [» ]
    2MAS X-ray 2.30 A/B/C/D 3-315 [» ]
    ProteinModelPortali Q27546.
    SMRi Q27546. Positions 3-314.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00606 .

    Miscellaneous databases

    EvolutionaryTracei Q27546.

    Family and domain databases

    Gene3Di 3.90.245.10. 1 hit.
    InterProi IPR015910. I/U_nuclsd_hydro_CS.
    IPR023186. Inosine/uridine_hydrolase.
    IPR001910. Inosine/uridine_hydrolase_dom.
    [Graphical view ]
    PANTHERi PTHR12304. PTHR12304. 1 hit.
    Pfami PF01156. IU_nuc_hydro. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53590. SSF53590. 1 hit.
    PROSITEi PS01247. IUNH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inosine-uridine nucleoside hydrolase from Crithidia fasciculata. Genetic characterization, crystallization, and identification of histidine 241 as a catalytic site residue."
      Gopaul D.N., Meyer S.L., Degano M., Sacchettini J.C., Schramm V.L.
      Biochemistry 35:5963-5970(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, MUTAGENESIS.
    2. "Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata."
      Degano M., Gopaul D.N., Scapin G., Schramm V.L., Sacchettini J.C.
      Biochemistry 35:5971-5981(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND CALCIUM IONS.

    Entry informationi

    Entry nameiIUNH_CRIFA
    AccessioniPrimary (citable) accession number: Q27546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 78 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3