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Protein

Inosine-uridine preferring nucleoside hydrolase

Gene

IUNH

Organism
Crithidia fasciculata
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, but has a preference for inosine and uridine as substrates.

Catalytic activityi

A purine nucleoside + H2O = D-ribose + a purine base.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Calcium
Binding sitei14 – 141Substrate1 Publication
Metal bindingi15 – 151Calcium
Metal bindingi126 – 1261Calcium
Binding sitei160 – 1601Substrate1 Publication
Binding sitei166 – 1661Substrate1 Publication
Binding sitei168 – 1681Substrate1 Publication
Active sitei241 – 2411
Metal bindingi242 – 2421Calcium

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleotide metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-uridine preferring nucleoside hydrolase (EC:3.2.2.-)
Short name:
IU-NH
Short name:
IU-nucleoside hydrolase
Alternative name(s):
Non-specific nucleoside hydrolase
Purine nucleosidase
Gene namesi
Name:IUNH
OrganismiCrithidia fasciculata
Taxonomic identifieri5656 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeCrithidia

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi241 – 2411H → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 315314Inosine-uridine preferring nucleoside hydrolasePRO_0000206810Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1
315
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi13 – 2412Combined sources
Beta strandi28 – 358Combined sources
Beta strandi37 – 404Combined sources
Helixi42 – 5514Combined sources
Beta strandi63 – 653Combined sources
Beta strandi71 – 733Combined sources
Helixi79 – 824Combined sources
Beta strandi84 – 874Combined sources
Helixi105 – 11511Combined sources
Beta strandi121 – 1255Combined sources
Helixi130 – 1389Combined sources
Helixi142 – 1454Combined sources
Beta strandi148 – 1525Combined sources
Beta strandi160 – 1645Combined sources
Helixi167 – 1704Combined sources
Helixi173 – 1819Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi186 – 1894Combined sources
Helixi191 – 1944Combined sources
Helixi201 – 2088Combined sources
Helixi213 – 23018Combined sources
Beta strandi236 – 2394Combined sources
Helixi242 – 2509Combined sources
Helixi252 – 2543Combined sources
Beta strandi255 – 2595Combined sources
Beta strandi262 – 2643Combined sources
Turni269 – 2735Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi288 – 2958Combined sources
Helixi297 – 31115Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MASX-ray2.50A/B3-315[»]
2MASX-ray2.30A/B/C/D3-315[»]
ProteinModelPortaliQ27546.
SMRiQ27546. Positions 3-314.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ27546.

Family & Domainsi

Sequence similaritiesi

Belongs to the IUNH family.Curated

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKKIILDCD PGLDDAVAIL LAHGNPEIEL LAITTVVGNQ TLAKVTRNAQ
60 70 80 90 100
LVADIAGITG VPIAAGCDKP LVRKIMTAGH IHGESGMGTV AYPAEFKNKV
110 120 130 140 150
DERHAVNLII DLVMSHEPKT ITLVPTGGLT NIAMAARLEP RIVDRVKEVV
160 170 180 190 200
LMGGGYHEGN ATSVAEFNII IDPEAAHIVF NESWQVTMVG LDLTHQALAT
210 220 230 240 250
PPILQRVKEV DTNPARFMLE IMDYYTKIYQ SNRYMAAAAV HDPCAVAYVI
260 270 280 290 300
DPSVMTTERV PVDIELTGKL TLGMTVADFR NPRPEHCHTQ VAVKLDFEKF
310
WGLVLDALER IGDPQ
Length:315
Mass (Da):34,326
Last modified:January 23, 2007 - v3
Checksum:iB88CF9DA41B53F43
GO

Mass spectrometryi

Molecular mass is 34194±4 Da from positions 2 - 315. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43371 Genomic DNA. Translation: AAC47119.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43371 Genomic DNA. Translation: AAC47119.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MASX-ray2.50A/B3-315[»]
2MASX-ray2.30A/B/C/D3-315[»]
ProteinModelPortaliQ27546.
SMRiQ27546. Positions 3-314.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00606.

Miscellaneous databases

EvolutionaryTraceiQ27546.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Inosine-uridine nucleoside hydrolase from Crithidia fasciculata. Genetic characterization, crystallization, and identification of histidine 241 as a catalytic site residue."
    Gopaul D.N., Meyer S.L., Degano M., Sacchettini J.C., Schramm V.L.
    Biochemistry 35:5963-5970(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, MUTAGENESIS.
  2. "Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata."
    Degano M., Gopaul D.N., Scapin G., Schramm V.L., Sacchettini J.C.
    Biochemistry 35:5971-5981(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND CALCIUM IONS.

Entry informationi

Entry nameiIUNH_CRIFA
AccessioniPrimary (citable) accession number: Q27546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.