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Protein

Inosine-uridine preferring nucleoside hydrolase

Gene

IUNH

Organism
Crithidia fasciculata
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, but has a preference for inosine and uridine as substrates.

Catalytic activityi

A purine nucleoside + H2O = D-ribose + a purine base.

Cofactori

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Calcium1
Binding sitei14Substrate1 Publication1
Metal bindingi15Calcium1
Metal bindingi126Calcium1
Binding sitei160Substrate1 Publication1
Binding sitei166Substrate1 Publication1
Binding sitei168Substrate1 Publication1
Active sitei2411
Metal bindingi242Calcium1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.2.3. 1365.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-uridine preferring nucleoside hydrolase (EC:3.2.2.-)
Short name:
IU-NH
Short name:
IU-nucleoside hydrolase
Alternative name(s):
Non-specific nucleoside hydrolase
Purine nucleosidase
Gene namesi
Name:IUNH
OrganismiCrithidia fasciculata
Taxonomic identifieri5656 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeCrithidia

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi241H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002068102 – 315Inosine-uridine preferring nucleoside hydrolaseAdd BLAST314

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi13 – 24Combined sources12
Beta strandi28 – 35Combined sources8
Beta strandi37 – 40Combined sources4
Helixi42 – 55Combined sources14
Beta strandi63 – 65Combined sources3
Beta strandi71 – 73Combined sources3
Helixi79 – 82Combined sources4
Beta strandi84 – 87Combined sources4
Helixi105 – 115Combined sources11
Beta strandi121 – 125Combined sources5
Helixi130 – 138Combined sources9
Helixi142 – 145Combined sources4
Beta strandi148 – 152Combined sources5
Beta strandi160 – 164Combined sources5
Helixi167 – 170Combined sources4
Helixi173 – 181Combined sources9
Beta strandi182 – 184Combined sources3
Beta strandi186 – 189Combined sources4
Helixi191 – 194Combined sources4
Helixi201 – 208Combined sources8
Helixi213 – 230Combined sources18
Beta strandi236 – 239Combined sources4
Helixi242 – 250Combined sources9
Helixi252 – 254Combined sources3
Beta strandi255 – 259Combined sources5
Beta strandi262 – 264Combined sources3
Turni269 – 273Combined sources5
Beta strandi275 – 277Combined sources3
Beta strandi288 – 295Combined sources8
Helixi297 – 311Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MASX-ray2.50A/B3-315[»]
2MASX-ray2.30A/B/C/D3-315[»]
ProteinModelPortaliQ27546.
SMRiQ27546.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ27546.

Family & Domainsi

Sequence similaritiesi

Belongs to the IUNH family.Curated

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKKIILDCD PGLDDAVAIL LAHGNPEIEL LAITTVVGNQ TLAKVTRNAQ
60 70 80 90 100
LVADIAGITG VPIAAGCDKP LVRKIMTAGH IHGESGMGTV AYPAEFKNKV
110 120 130 140 150
DERHAVNLII DLVMSHEPKT ITLVPTGGLT NIAMAARLEP RIVDRVKEVV
160 170 180 190 200
LMGGGYHEGN ATSVAEFNII IDPEAAHIVF NESWQVTMVG LDLTHQALAT
210 220 230 240 250
PPILQRVKEV DTNPARFMLE IMDYYTKIYQ SNRYMAAAAV HDPCAVAYVI
260 270 280 290 300
DPSVMTTERV PVDIELTGKL TLGMTVADFR NPRPEHCHTQ VAVKLDFEKF
310
WGLVLDALER IGDPQ
Length:315
Mass (Da):34,326
Last modified:January 23, 2007 - v3
Checksum:iB88CF9DA41B53F43
GO

Mass spectrometryi

Molecular mass is 34194±4 Da from positions 2 - 315. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43371 Genomic DNA. Translation: AAC47119.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43371 Genomic DNA. Translation: AAC47119.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MASX-ray2.50A/B3-315[»]
2MASX-ray2.30A/B/C/D3-315[»]
ProteinModelPortaliQ27546.
SMRiQ27546.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00606.
BRENDAi3.2.2.3. 1365.

Miscellaneous databases

EvolutionaryTraceiQ27546.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIUNH_CRIFA
AccessioniPrimary (citable) accession number: Q27546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.